| comparative serological studies with mammalian adenoviruses. | nine serotypes of bovine adenovirus, five serotypes of ovine adenovirus, and four serotypes of porcine adenovirus were compared in reciprocal cross-neutralisation tests, to determine if viruses isolated from different species were indeed distinct serotypes. in addition, the above serotypes were tested for possible antigenic relationships with 28 of 32 human adenoviruses, by one-way cross neutralisation tests with human adenovirus antisera. the results indicated that all viruses tested were disti ... | 1976 | 61747 |
| porcine adenovirus isolated from pigs with toxoplasmosis. | | 1978 | 216930 |
| the inactivation of viruses in cattle and pig slurry by aeration or treatment with calcium hydroxide. | porcine enterovirus type 2 or porcine adenovirus type 3 were seeded into samples of pig slurry, and a bovine enterovirus was seeded into cattle slurry, and samples of the slurry were aerated in the laboratory for 21 days. the viruses were inactivated more rapidly in the aerated slurry than in control slurry which was not aerated. the difference in inactivation rate was greatest for the porcine adenovirus and least for the bovine enterovirus. inactivation of the porcine enterovirus in aerated dis ... | 1979 | 219109 |
| a seroepizootiologic study of five viruses in a swine-evaluation station. | this serologic study was done to gain information on the spread, maintenance, and effect upon performance of five porcine viruses. blood samples were taken from two groups of 8- to 11-week-old pigs from a large number of indiana swine herds in a performance-testing station 1 week after entry, 7 weeks after entry (one group only), and at slaughter. the sera were tested by indirect fluorescent antibody tests for antibodies to transmissible gastroenteritis virus (tgev), swine influenza virus (siv), ... | 1979 | 230762 |
| molecular cloning and physical mapping of the dna of bovine adenovirus serotype 4; study of the dna homology among bovine, human and porcine adenoviruses. | the dna of bovine adenovirus (bav) serotype 4, a member of the subgroup 2 bavs, has been cloned and mapped with 11 restriction enzymes. southern blot hybridizations probed by a clone containing about 50% of the bav-4 genome revealed a very strong and extended dna sequence homology amongst the members of subgroup 2, but no homology was detectable to the subgroup 1 bovine, or any of those human (hav) and porcine adenovirus serotypes examined. these findings were strengthened by reciprocal hybridiz ... | 1990 | 2307965 |
| isolation of porcine adenovirus from the respiratory tract of pigs in japan. | | 1990 | 2348602 |
| the interferon sensitivity of selected porcine viruses. | the objective of this study was to compare the sensitivity of 11 porcine viruses to the antiviral effects of porcine interferon-alpha in serum from piglets which had been infected 19 h previously with transmissible gastroenteritis virus, and of porcine interferon-beta prepared in pk-15 cells by induction with polyinosinic:polycytidylic acid, in yield reduction assays in pig kidney cells which were treated with interferon before virus challenge, and both before and after virus challenge. the most ... | 1989 | 2492445 |
| genome typing of three serotypes of porcine adenovirus. | porcine enteric adenovirus serotypes 1-3, previously shown to be distinct antigenically, were purified by density gradient centrifugation, and the dna was extracted. fragments generated by digestion with restriction endonucleases bamhi, ecori, hindiii, and paer7 were separated by electrophoresis and compared with human adenovirus type 2 as a control. the results demonstrated that the three porcine viruses were closely related to each other, although distinguishable, and distinct by restriction e ... | 1989 | 2550389 |
| pathologic changes caused by transplacental infection with an adenovirus-like agent in pigs. | five hysterectomy-derived colostrum-deprived pigs housed in individual cages with positive ventilation developed severe skin cyanosis and edema of the subcutaneous tissues in the submandibular, thoracic, and abdominal regions. the 5 pigs were killed 7 to 10 days after birth. eosinophilic intranuclear inclusion bodies were found in endothelial cells of capillary and small blood vessels throughout the body. ultrastructurally, nuclei of these affected endothelial cells contained small crystalline a ... | 1985 | 2988380 |
| intranuclear inclusion bodies in the intestinal epithelium of pig foetuses experimentally infected with porcine adenovirus. | | 1967 | 4291505 |
| experimental infection of pigs with 2 strains of porcine adenovirus. | | 1967 | 4291587 |
| distribution of a porcine adenovirus after inoculation of experimental animals. | | 1968 | 4314368 |
| the activity of some chemical disinfectants against talfan virus and porcine adenovirus type 2. | | 1971 | 4323383 |
| intrapulmonary lymphoid tissue of pigs exposed to aerosols of haemolytic streptococcus group l and porcine adenovirus. | | 1971 | 4326294 |
| meningo-encephalitis in gnotobiotic pigs inoculated intransally and orally with porcine adenovirus 4. | | 1972 | 4339608 |
| the isolation of a porcine adenovirus in queensland. | | 1971 | 5102654 |
| ultrastructural alterations of tissue cultures infected with a pathogenic porcine adenovirus. | | 1968 | 5692209 |
| effects of a pathogenic porcine adenovirus on porcine kidney cell culture. | | 1969 | 5780385 |
| replication of porcine enteric adenoviruses in vivo. | the replication of an enteropathogenic porcine adenovirus in the intestinal epithelial cells of naturally and experimentally piglets has been studied by transmission electron microscopy (tem) and by immunoperoxidase (ip) staining of paraffin sections. three types of intranuclear inclusion bodies were observed. viral particles appeared to be assembled from electron dense crescents which seemed to originate from type ii intranuclear inclusion bodies. virus accumulated in the nucleus of infected ce ... | 1981 | 6271102 |
| sequential pathological study of experimental porcine adenovirus enteritis. | the sequential enteric lesions of experimental porcine adenovirus strain 6618 infection were studied in 18 hysterotomy-derived, colostrum-deprived piglets, by histology, transmission electron microscopy, and an immunoperoxidase technique. viral particles could be seen in altered epithelial cells of the lower small intestine from 24 hours until 16 days after inoculation. with the immunoperoxidase technique, viral antigen could be found in epithelial cells at 45 days after challenge. destruction o ... | 1982 | 6280363 |
| cultivation of a porcine adenovirus in porcine thyroid cell cultures. | the porcine adenovirus type 4 was adapted to grow in porcine thyroid cell cultures. a readily recognizable cytopathic effect appeared in these cells as soon as the first passage of the virus and complete degeneration of the monolayers was obtained after only 72 hours post-infection at the fourth passage. a viral yield of 10(6.0) tcid50/ml was calculated after the third passage. the virus was purified by cscl density gradient centrifugation and was shown to possess a buoyant density of 1.33 g/ml. ... | 1984 | 6375960 |
| prevalence of antibodies to porcine adenovirus in swine by indirect fluorescent antibody test. | an indirect fluorescent antibody test was developed for routine identification of a porcine adenovirus and its specific antibody. two specific-pathogen-free young pigs were inoculated with the viral antigen prepared in continuous porcine kidney cell cultures, and their sera were used as an antibody reagent to standardize the test. sera of adult pigs with respiratory problems, obtained from pig farms in quebec, were tested for antibodies to this virus; 83 of 540 sera tested (15.2%) were found to ... | 1984 | 6388434 |
| [further studies on the etiology of atrophic rhinitis in pigs. vi. investigations on virus isolation from nasal mucous and the prevalence of antibodies against porcine adenovirus serotypes in pig sera]. | | 1980 | 7406836 |
| restriction endonuclease analysis and physical mapping of the genome of porcine adenovirus type 5. | the hnf61 and hnf70 isolates of porcine adenovirus type 5 (pav-5) were cultivated in pk-15 cells, and viral dna was extracted from the infected cells by a modified hirt procedure. the dnas were digested by each of 9 restriction endonucleases, and fragments representing the entire genomes were cloned. based on the sizes of the restriction enzyme fragments, the genome of each isolate was estimated to be 33.2 kb. physical maps for the 9 restriction endonucleases were constructed. the physical maps ... | 1995 | 7483821 |
| isolation of porcine adenovirus as a candidate of 5th serotype. | a strain of cytopathic virus, named strain tg/k79, was isolated from the brain of a newborn piglet, pure hampshire breed, which died shortly after birth. the physicochemical property of virus was considered to be that of the family adenoviridae. a significant difference between our isolated and 4 reference porcine adenoviruses was demonstrated by cross-seroneutralization test. differences between tg/k79 and other porcine adenoviruses were also seen in electrophoretic patterns of viral dna in aga ... | 1995 | 7608865 |
| sequence analysis of putative pviii, e3 and fibre regions of porcine adenovirus type 3. | sequence analysis of a region of the genome of porcine adenovirus type 3 from map unit 79.5 to map unit 92 was performed. homology studies revealed genes coding for the hexon-associated protein pviii on the left and for the fibre protein on the right of the sequenced region. by analogy with the genomic organization of other adenoviruses, the 1179 bp sequence between the pviii and fibre open reading frames, extending from map unit 81.3 to map unit 84.7, was identified as the equivalent of the e3 ... | 1995 | 7625129 |
| molecular cloning and physical mapping of porcine adenovirus types 1 and 2. | the 25r strain of porcine adenovirus type 1 (pav-1) and the a47 strain of pav-2 were propagated in st cells, and dna was extracted from the infected cells by a modified hirt method. the dna of each virus was digested by each of nine restriction endonucleases, and restriction enzyme fragments representing the entire genome were cloned. the genomic size of each virus was approximately 33 kb. physical maps for the nine restriction endonucleases were constructed from the results of double digestion ... | 1995 | 7646345 |
| identification and sequence analysis of the e1 genomic region of a porcine adenovirus. | the complete nucleotide sequence of the putative early transcriptional region 1 (e1) of the genome of nadc-1, a porcine adenovirus, was determined. the e1 region of nadc-1 was found to be 3658 bp and located between 0 and 11.2 map units. twelve potential open reading frames (orfs) and five polyadenylation signals were identified in the r strand. the nucleotide sequence and each predicted amino acid sequence were compared to sequences available on a number of databases by a blast search and compa ... | 1995 | 7653103 |
| comparison of the inverted terminal repetition sequences from five porcine adenovirus serotypes. | the nucleotide sequences of the region of inverted terminal repetition from representative strains of all five porcine adenovirus (pav) serotypes were determined and analyzed. the first 17 nucleotides of this region were identical in pav-1 to 3 and pav-5, and 10 bp of identical sequence was found in all the pavs. the closest relationships were among pav-1 to 3, which shared more common sequences than the other serotypes. pav-4 had the longest inverted terminal repeat reported for any adenovirus. ... | 1995 | 7676637 |
| genomic cloning and restriction site mapping of a porcine adenovirus isolate: demonstration of genomic stability in porcine adenovirus. | restriction endonuclease maps were constructed for the genome of a porcine adenovirus (pav), nadc-1, which was isolated in 1972 from an adult swine. genomic dna libraries of nadc-1 bam hi, eco ri/bam hi, and sph i fragments were cloned into puc-18. using the cloned nadc-1 bam hi and eco ri/bam hi fragments as probes, southern blot hybridizations were performed to human adenovirus 2 (ad-2) restriction fragments to determine the left-to-right orientation of the bam hi and eco ri/bam hi fragments. ... | 1993 | 7903036 |
| restriction endonuclease analysis and molecular cloning of porcine adenovirus type 3. | the 6618 strain of porcine adenovirus type 3 was cultivated in swine testis cells, and viral dna was extracted from the infected cells by a modified hirt procedure. the dna was digested by each of 13 restriction endonucleases, and the number of cleavage sites which were identified varied from 1 to 17. the size of the porcine adenovirus type 3 genome, estimated from the sizes of the restriction enzyme fragments, was approximately 35 kb. fragments representing the entire genome were cloned. physic ... | 1993 | 8150597 |
| sequence analysis of putative e3, pviii, and fiber genomic regions of a porcine adenovirus. | the complete nucleotide sequence of the putative early transcriptional region 3 (e3), plus the hexon-associated polypeptide viii (pviii) gene, and the n-terminus of the fiber protein gene of a porcine adenovirus isolate, nadc-1, was determined. the e3 region of nadc-1 was found to be 1879 bp and located between 80 and 85.8 map units. eight open reading frames (orfs) and three polyadenylation signals were identified in the r strand. the amino acid sequences predicted to be encoded by orfs 1 and 8 ... | 1994 | 8165866 |
| potential viral vectors for the stimulation of mucosal antibody responses against enteric viral antigens in pigs. | four viruses were compared for their ability to induce an intestinal antibody response in piglets. antibodies were not detected in response to oral vaccination with either fowlpox virus or a baculovirus (bv). simultaneous oral dosing and parenteral inoculation with high concentrations of bv in an oil emulsion adjuvant induced high levels of circulating virus neutralising (vn) antibodies, and also low levels of intestinal antibodies when booster doses of virus were given. in response to oral vacc ... | 1993 | 8393209 |
| bovine adenovirus type 10: properties of viruses isolated from cases of bovine haemorrhagic enterocolitis. | the isolation, serological classification and growth properties of adenoviruses isolated from fatal cases of haemorrhagic enterocolitis in calves are described. four viruses, from different submissions, were isolated in cultures of calf testis cells and were identified as adenoviruses by electron microscopy. the four isolates were serologically identical and were classified as bovine adenovirus type 10 in cross-neutralisation tests with other bovine, ovine and porcine adenovirus species. their g ... | 1996 | 8734506 |
| genomic location and nucleotide sequence of a porcine adenovirus penton base gene. | the putative penton base gene of a porcine adenovirus serotype 3 (pav3) has been identified, cloned and sequenced. the genomic location of the pav3 penton base was deduced by probing a southern blot with a polymerase chain reaction generated product containing the human adenovirus type 2 (hav2) penton base gene. sequencing revealed an open reading frame (orf) of 1527 nucleotides coding for a polypeptide of 509 amino acids. however, cdna analysis indicated an acceptor splice site one nucleotide u ... | 1996 | 8774695 |
| sequence analysis of the fiber genomic region of a porcine adenovirus predicts a novel fiber protein. | the complete nucleotide sequence of the putative fiber protein of a porcine adenovirus isolate, nadc-1, was determined. the coding sequence for the fiber protein was found to be 2112 nucleotides, predicting a 703 amino acid protein with a calculated molecular mass of 76,681 da. the coding sequence is located between 86 and 92.5 map units. a polyadenylation signal was found 44 bases downstream of the stop codon. northern hybridization analysis identified a band at 2.4 kb, which likely includes th ... | 1995 | 8837892 |
| porcine adenoviruses types 1, 2 and 3 have short and simple early e-3 regions. | the nucleotide sequence of the e-3 region genes, the hexon associated protein pviii genes, and part of the fiber genes coding for the n-terminal tail regions, of porcine adenovirus (pav) types 1 and 2 were determined. the sizes of the e-3 regions were found to be 1162 and 1222 bp, respectively. the five open reading frames (orf) encoded within the sequenced regions of pav types 1 and 2 shared a high degree of homology with the published sequences of the corresponding orfs of pav-3. the e-3 regio ... | 1996 | 8864200 |
| detection of homologous dna sequences in animal adenoviruses by polymerase chain reaction. | a primer pair originally designed for the universal detection of human adenovirus (hav) serotypes of all subgenera was modified then tested and found feasible for the detection of different bovine, ovine, and porcine adenovirus (bav, oav, and pav, respectively) serotypes. apparently, in the examined viruses, parts of the dna sequence coding for the basal part of the hexon protein are conserved enough for being applicable in polymerase chain reaction (pcr) as primers. positive amplification could ... | 1996 | 8908748 |
| nucleotide and amino acid sequence analysis of the porcine adenovirus 23k protein. | the genomic location of the viral encoded protease (23k) of porcine adenovirus serotype 3 (pav3) was determined and the appropriate fragment cloned and sequenced. an open reading frame (orf) coding for a polypeptide of 203 amino acids and a calculated molecular weight of 23.3 kda was found. the orf was situated in a position similar to that of the human adenovirus 23k, that is, between a putative stop codon for the hexon gene and the polyadenylation signal, aataaa, for the late region 3. amino a ... | 1996 | 8912929 |
| beneficial use of inactivated porcine adenovirus vaccine and antibody response of young pigs. | an inactivated adenovirus vaccine experimentally prepared was inoculated into young pigs against the adenovirus infection newly appeared in japan. sero-neutralizing titers of the paired sera collected from 50 pigs in the interval of 3 weeks was measured. the mean of antibody titers of post-sera was 45 times higher than that of pre-sera. | 1997 | 9037675 |
| characterization of the early region 4 of porcine adenovirus type 3. | the nucleotide sequence of a 3028 bp dna segment, located between map co-ordinates 100 and 92 in the genome of porcine adenovirus type 3 (pav-3), was determined. the segment includes the entire early region 4 (e-4) and the right inverted terminal repeat sequences. there were two tata boxes and one canonical polyadenylation signal on the 1 strand. homology searches of the genbank data base for the predicted amino acid sequences revealed that, of the eight open reading frames (orfs) on the 1 stran ... | 1997 | 9354276 |
| completion of the dna sequence of mouse adenovirus type 1: sequence of e2b, l1, and l2 (18-51 map units). | the dna sequence of 9991 nt, corresponding to 18-51 map units of mouse adenovirus type 1 (mav-1), was determined, completing the sequence of the larsen strain of mav-1. the length of the complete mav-1 genome is 30,946 nucleotides, consistent with previous experimental estimates. the 18-51 map unit region encodes early region 2b proteins necessary for adenoviral replication as well as late region l1 and l2 structural and packaging proteins. sequence comparison in this region with human adenoviru ... | 1997 | 9381795 |
| nucleotide and amino acid sequence analysis of the 100k protein of a serotype 3 porcine adenovirus. | the genomic region between map units 69 and 78 of a type 3 porcine adenovirus (pav3) was sequenced and analysed. an open reading frame (orf) of 2514 nucleotides encoding a polypeptide of 838 amino acids and approximately 94.1 kda was found. the size and location of the orf suggested it was the pav3 homologue of the 100k gene and this was confirmed by nucleotide sequence comparison with the 100k of human adenovirus type 2. amino acid sequence alignment of the predicted polypeptide with the sequen ... | 1997 | 9522122 |
| nucleotide sequence and transcription map of porcine adenovirus type 3. | the complete nucleotide sequence of porcine adenovirus type 3 was determined and a transcriptional map for the genome was constructed. the size of the genome is 34094 bp in length with an unusually high g + c content (63.7%), the highest thus far reported for any adenovirus. overall organization of the genome is similar to that for previously sequenced adenoviral dnas, but there also were distinct differences. the late regions genes are organized into six families, instead of five as they are in ... | 1998 | 9837805 |
| sequence and transcription map analysis of early region-1 of porcine adenovirus type-3. | the nucleotide sequence of a region of the genome of porcine adenovirus-3 (pav-3) between map units 1 and 12.2 was determined. the sequenced region included four major open reading frames, and several transcription control elements. homology studies, using the deduced amino acid sequences of the open reading frames, revealed genes coding for the e1a, e1b 202r, e1b 474r and pix proteins. the region was characterized by northern blot analysis and sequencing of cdna clones. in pav-3, the e1a region ... | 1998 | 9879766 |
| development of porcine adenovirus-3 as an expression vector. | porcine adenovirus-3 (pav-3) was developed as an expression vector using homologous recombination in escherichia coli bj 5183. as a prerequisite, the complete genome of pav-3 was first introduced as a paci restriction fragment into a bacterial plasmid. the plasmid, when paci restricted and transfected into swine testicular cells, produces an infectious virus. the potential of this procedure was demonstrated by the construction of several pav-3 recombinants. part of the e3 region, which is noness ... | 1999 | 10091994 |
| genomic location and nucleotide sequence of a serotype 3 porcine adenovirus hexon gene. | the putative hexon gene of a porcine adenovirus serotype 3 (pav3) has been identified, cloned and the nucleotide sequence determined. the genomic location of the pav3 hexon gene was determined and an open reading frame (orf) encoding a polypeptide of 939 amino acids identified. comparison of the nucleotide sequence of the putative pav3 hexon gene with the sequence of the hav2 hexon gene returned an overall identity of approximately 63%. a stop codon 144 nucleotides upstream and a start codon 18 ... | 1999 | 10446655 |
| porcine adenovirus-3 as a helper-dependent expression vector. | porcine adenovirus has been proposed as a potential vector for generating novel and effective vaccines for pigs. as a prerequisite for the generation of helper-dependent porcine adenovirus-3 (pav-3) vectors, two e1-complementing porcine cell lines expressing e1 proteins of human adenovirus-5 (hav-5) were made. these cell lines could be efficiently transfected with dna and allowed the rescue and propagation of a pav-3 recombinant, pav201, containing a 0.597 kb e3 deletion and a 0.803 kb e1a delet ... | 1999 | 10580052 |
| vaccination with a single dose of a recombinant porcine adenovirus expressing the classical swine fever virus gp55 (e2) gene protects pigs against classical swine fever. | a recombinant porcine adenovirus (rpav) with the gp55 (e2) gene from the classical swine fever virus (csfv) 'weybridge' strain inserted into the right hand end of the pav serotype 3 (pav3) genome was constructed. expression of gp55 was directed by the major late promoter and tri-partite leader sequences located and cloned from pav3. no compensatory deletions of pav dna sequences were made. vaccination of outbred pigs with a single dose of the recombinant virus (rpav-gp55) resulted in complete pr ... | 2000 | 10590324 |
| sequence analysis of porcine adenovirus type 3 e1 region, pix and piva2 genes, and two novel open reading frames. | the porcine adenovirus type 3 (pad3) genome between map units 0 and 13.7 was sequenced and compared with similar regions of other adenoviruses. this region consists of the left inverted terminal repeat sequences involved in dna packaging, the entire early region 1 (e1) and the protein ix (pix) transcription unit. the lower strand contains the c-terminal end of iva2 of the e2a transcriptional unit and two novel open reading frames (orfs). the e1 transcription unit consists of orfs for proteins ho ... | 2000 | 10773731 |
| circumvention of vector-specific neutralizing antibody response by alternating use of human and non-human adenoviruses: implications in gene therapy. | to determine whether non-human adenovirus-specific antibodies are cross-neutralizing, rabbit and mouse anti-human adenovirus type 5 (had5), anti-bovine adenovirus type 3 (bad3), and anti-porcine adenovirus type 3 (pad3) sera were used in cross-virus neutralization assays. adenovirus neutralizing antibodies were found to be virus-specific, suggesting that virus neutralizing epitope differs significantly in had5, bad3, and pad3. to further investigate whether immunity to an had5-derived vector cou ... | 2000 | 10873758 |
| sequence analysis and deletion of porcine adenovirus serotype 5 e3 region. | a 3000 basepair (bp) region corresponding to the e3 region, the flanking pviii and part of the fiber protein genes, of the two prototype strains (hnf-61 and hnf-70) of porcine adenovirus serotype five (padv-5) was sequenced. a potential e3 promoter and poly-a signals were identified. the size of the e3 region was 2039 (strain hnf-61) and 2020 bp (strain hnf-70) the largest e3 so far reported among padvs. three open reading frames (orf2-4) were identified within the e3 region. based on the predic ... | 2000 | 10958982 |
| construction and characterization of recombinant porcine adenovirus serotype 5 expressing the transmissible gastroenteritis virus spike gene. | five recombinant porcine adenoviruses of serotype 5 (padv-5) carrying the full-length or the 5' 2.2 kb half of the transmissible gastroenteritis virus (tgev) spike (s) gene were generated by homologous recombination in e. coli strain bj5183 cells and subsequent transfection of swine testicle cells. the foreign genes were inserted into the e3 region of padv-5. one recombinant virus had no deletion in the e3 region, whereas a 1.2 kb fragment was removed from the e3 region in the remainder of the r ... | 2001 | 11125171 |
| the complete nucleotide sequence of porcine adenovirus serotype 5. | the complete nucleotide sequence of porcine adenovirus serotype 5 (padv-5) has been determined and the putative genomic map was constructed. the size of the genome was found to be 32621 nucleotides. twenty-eight putative orfs were identified by their homology to other adenovirus or other virus and eukaryotic genes. several special protein sequence motifs were identified by their homology to similar protein motifs. the putative promoter regions, polyadenylation and splice sites were predicted and ... | 2001 | 11172093 |
| a prime-boost vaccination strategy using naked dna followed by recombinant porcine adenovirus protects pigs from classical swine fever. | weaned pigs (6-week-old) and 7-day-old pre-weaned piglets were vaccinated with naked plasmid dna expressing the gp55/e2 gene from classical swine fever virus (csfv). both groups of pigs were then given a booster dose of recombinant porcine adenovirus expressing the gp55 gene (rpav-gp55). following challenge with csfv, 100% of weaned pigs and 75% pre-weaned piglets were protected from disease. weaned pigs given a single dose of rpav-gp55 were also protected, but showed a slight increase in temper ... | 2001 | 11295331 |
| vaccination of pigs with a recombinant porcine adenovirus expressing the gd gene from pseudorabies virus. | five week old, commercially available large white pigs were vaccinated with either a single dose or two doses of a recombinant porcine adenovirus expressing the glycoprotein d gene from pseudorabies virus (prv). pigs were monitored for the development of serum neutralizing antibodies to prv and challenged 3 weeks after final vaccination. prior to challenge, pigs given 2 doses of the vaccine demonstrated boosted levels of antibody compared with those given a single dose, and all surviving pigs ha ... | 2001 | 11395210 |
| oral and sub-cutaneous vaccination of commercial pigs with a recombinant porcine adenovirus expressing the classical swine fever virus gp55 gene. | a recombinant porcine adenovirus expressing the classical swine fever virus (csfv) gp55/e2 gene was administered to commercially available pigs via oral or subcutaneous routes and their susceptibility to oral and subcutaneous challenge with csfv was determined. 100% of animals vaccinated and challenged subcutaneously were protected. in the groups of pigs vaccinated either orally or subcutaneously and then challenged orally, 60% of animals were protected. before challenge, neutralising antibodies ... | 2001 | 11699963 |
| characterization of dna binding protein of porcine adenovirus type 3. | to identify and characterize the protein encoded by the e2a region of porcine adenovirus (pav)-3, dna sequence coding for a portion (amino acids 102-457) of the dna binding protein (dbp) open reaching frame was cloned and expressed in escherichia coli as a fusion protein with glutathione s-transferase protein of schistosoma japonica. the affinity-purified fusion protein was used to immunize rabbits. immunoprecipitation/western blot analysis demonstrated that the antisera specifically recognized ... | 2001 | 11805441 |
| analysis of early region 1 of porcine adenovirus type 3. | to identify the proteins encoded by the porcine adenovirus 3 (pav-3) e1 region, rabbit antisera were prepared using a bacterial fusion protein encoding e1a, e1b(small), or e1b(large) protein. sera against e1a, e1b(small), and e1b(large) immunoprecipitated a protein of 35, 23, and 53 kda, respectively, in in vitro translated and transcribed mrna and pav-3 infected cells. to determine the role of e1 proteins in pav-3 replication, we constructed vectors with a deletion(s) in the e1 region. mutant p ... | 2001 | 11878877 |
| sequence analysis of porcine adenovirus serotype 5 fibre gene: evidence for recombination. | the nucleic acid and deduced amino acid sequence of the fibre gene of the hnf-61 strain of porcine adenovirus serotype 5 (padv-5) was determined and compared to that of the hnf-70 strain of the same serotype (nagy et al., j gen virol 82, 525-529, 2001) and also to adenovirus fibre genes from the genera mastadenovirus and atadenovirus. the putative hnf-61 and hnf-70 proteins were similar to each other, with 90% amino acid identity. conserved amino acid sequences described for mastadenovirus fibre ... | 2002 | 12018710 |
| continuous porcine cell lines developed from alveolar macrophages: partial characterization and virus susceptibility. | porcine monomyeloid cell lines were established following transfection of primary porcine alveolar macrophage cultures with plasmid psv3neo, carrying genes for neomycin resistance and sv40 large t antigen. the parental clone 3d4 exhibited a relatively rapid doubling time (25.5 h), high plating efficiency and mixed phenotype with respect to growth on a solid support. single cell cloning of the 3d4 parent resulted in establishment of several cell lines; three of them designated 3d4/2, 3d4/21 and 3 ... | 2002 | 12088830 |
| a recombinant e1-deleted porcine adenovirus-3 as an expression vector. | replication-defective e1-deleted porcine adenoviruses (pavs) are attractive vectors for vaccination. as a prerequisite for generating pav-3 vectors containing complete deletion of e1, we transfected vido r1 cells (fetal porcine retina cells transformed with e1 region of human adenovirus 5) with a construct containing pav-3 e1b(large) coding sequences under the control of hcmv promoter. a cell line named vr1bl could be isolated that expressed e1b(large) of pav-3 and also complemented pav214 (e1a+ ... | 2003 | 12954206 |
| demonstration of a porcine adenovirus by electron microscopy. | | 1965 | 14277070 |
| electron microscopy of a porcine adenovirus. | | 1965 | 14344269 |
| characterization of cis-acting sequences involved in packaging porcine adenovirus type 3. | encapsidation of adenovirus dna involves specific interactions between cis-acting genomic dna sequences and trans-acting proteins. the cis-acting packaging domain located near the left inverted terminal repeat is composed of a series of redundant but not functionally equivalent motifs. such motifs are made up of the consensus sequence 5'-tttgn(8)cg-3' and 5'-tttg/a-3' in human adenovirus 5 (hav-5) and canine adenovirus-2 (cav-2), respectively. to gain comparative insight into adenovirus encapsid ... | 2003 | 14554092 |
| protection of pigs against 'in contact' challenge with classical swine fever following oral or subcutaneous vaccination with a recombinant porcine adenovirus. | a recombinant porcine adenovirus expressing the classical swine fever virus (csfv) gp55 gene (rpadv-gp55) was administered to commercially available outbred pigs via the subcutaneous or oral route and their susceptibility to 'in contact' challenge with classical swine fever determined. animals vaccinated subcutaneously with a single dose of recombinant vaccine and challenged by 'in contact' exposure were protected from disease, whereas pigs given an equivalent single oral dose did not survive ch ... | 2003 | 14602208 |
| porcine adenovirus type 3 e1 transcriptional control region contains a bifunctional regulatory element. | we identified a bifunctional regulatory element located between nt 374 and 431 upstream of tata box of porcine adenovirus (pav) 3 e1a promoter. deletion of the element dramatically reduced the steady-state level of e1a mrna, but increased that of e1b, which lies immediately downstream of e1a. the mutant virus displayed defective replication at early times of infection, but replicated nearly as efficiently as wild-type pav-3 at late times of infection. this defect was complemented with coinfectin ... | 2004 | 14972533 |
| viral rnas detected in virions of porcine adenovirus type 3. | it has been demonstrated that cellular and viral rnas were packaged in the virions of human cytomegalovirus (cmv) and herpes simplex virus 1 (hsv 1), members of the herpesviridae family, both of which are enveloped double-stranded dna viruses. here, we provide evidence suggesting that rnas are packaged in the virions of porcine adenovirus type 3 (padv-3), which is a member of the adenoviridae family, a non-enveloped double-stranded dna virus. the rnas packaged in padv-3 virions were enriched in ... | 2004 | 15051396 |
| analysis of early region 4 of porcine adenovirus type 3. | the early region 4 (e4) of porcine adenovirus (padv)-3, located at the right-hand end of the genome is transcribed in a leftward direction and has the potential to encode seven (p1-p7) open reading frames (orfs). to determine the role of each protein in viral replication, we constructed full-length padv-3 genomic clones containing deletions of individual e4 orf or combined deletions of the neighboring orfs. transfection of swine testicular (st) cells with individual e4 mutant plasmid dnas genera ... | 2004 | 15246655 |
| cis-acting packaging motifs of porcine adenovirus type 3. | the cis-acting packaging domain is required for selective encapsidation of adenovirus dna into preformed empty capsids late in the viral life cycle. earlier, it was demonstrated that the cis-acting packaging domain of porcine adenovirus type (padv)-3 is located between nucleotide position (nt) 212 and 531 at the left end genome which contains six at/gc rich motifs. removal of packaging domain from left end to the right end of the genome produced a viable mutant virus suggesting that the identifi ... | 2004 | 15246658 |
| porcine adenoviral vectors evade preexisting humoral immunity to adenoviruses and efficiently infect both human and murine cells in culture. | preexisting immunity against human adenoviruses (had) limits the efficiency of transduction of had vectors in humans. in addition, development of a vector-specific immune response after the first inoculation with a had vector further lowers vector uptake following readministration. we investigated the usefulness of porcine adenovirus serotype 3 (pad3)-based vectors as a supplement to had vectors. here we demonstrate that preexisting had-specific neutralizing antibodies in humans do not cross-neu ... | 2004 | 15351486 |
| porcine adenovirus serotype 3 internalization is independent of car and alphavbeta3 or alphavbeta5 integrin. | nonhuman adenoviruses including porcine adenovirus serotype 3 (pad3) are emerging vectors for gene delivery. pad3 efficiently transduces human and murine cells in culture, and circumvents preexisting humoral immunity in humans. the coxsackievirus-adenovirus receptor (car) serves as a primary receptor and alphavbeta3 or alphavbeta5 integrin as a secondary receptor for several human adenovirus (had) subtypes including had5. in this study, we deduced the role of car, alphavbeta3 or alphavbeta5 inte ... | 2005 | 15661148 |
| porcine adenovirus as a delivery system for swine vaccines and immunotherapeutics. | porcine adenovirus (padv) has many qualities which make it an ideal choice for use as a delivery vector in swine. it is a low grade pathogen, present almost world-wide in a number of serotypes varying in their virulence and tissue tropism, which may allow for serotype specific vaccine targeting. padv is species specific having only been isolated from swine, reducing the possibility of its spread to other animals or man following administration. when engineered to contain a foreign gene, recombin ... | 2005 | 15683761 |
| promoter activity of left inverted terminal repeat and downstream sequences of porcine adenovirus type 3. | early region 1 (e1) of porcine adenovirus type 3 (padv-3) consists of e1a and e1b transcription units. the authentic promoter region of e1a contains a tata box at nucleotide position (nt) 449 and a bifunctional regulatory element between nt 374 and 431, which enhances the transcription of e1a, but represses that of e1b. here, we investigated the role of the left inverted terminal repeat (itr) and its downstream sequences (between nt 151 and 312) in the transcription of early viral genes, and vir ... | 2004 | 15826912 |
| a newly identified interaction between iva2 and pviii proteins during porcine adenovirus type 3 infection. | the adenovirus iva2 is an intermediate viral gene product that appears to perform multiple essential roles in viral infection. using iva2 as bait in the yeast two-hybrid system, we screened selected open reading frames (orfs) of porcine adenovirus (padv)-3 for potential interaction with iva2. interestingly, pviii showed specific interaction with iva2. the yeast two-hybrid findings were validated by gst pull-down assays, in vitro binding studies employing cell-free coupled transcription-translati ... | 2005 | 15866071 |
| bovine adenovirus type 3 internalization is independent of primary receptors of human adenovirus type 5 and porcine adenovirus type 3. | usefulness of adenoviral vectors derived from human adenovirus (had) type 5 (had5) is mainly limited by wide prevalence of preexisting anti-had5 immunity as well as non-specific tissue tropism of these vectors. as an alternative, non-human adenoviral vectors including bovine adenovirus type 3 (bad3) are currently being investigated. non-prevalence of bad3 in humans and its ability to evade preexisting had immunity are some of the features that make bad3 a promising vector for human gene delivery ... | 2005 | 15883040 |
| transcription mapping and characterization of proteins produced from early region 4 of porcine adenovirus type 3. | the early region 4 (e4) of porcine adenovirus 3 (padv-3) was characterized by northern blot, rapid amplification of cdna ends (race), rt-pcr and cdna sequence analysis. northern blot analysis revealed three different classes of transcripts, which appeared and peaked at different times post-infection. the rt-pcr, race and cdna sequence analysis identified nine major e4 transcripts, all of which shared a 107-bp 5' leader sequence and a 126-bp 3' terminus. these transcripts have one to three intron ... | 2007 | 17122893 |
| prevalence of antibodies to swine influenza virus, porcine adenovirus type 4 and haemophilus pleuropneumoniae in quebec pig farms with respiratory problems. | the prevalence of antibodies to three etiological agents involved in swine pneumonia was determined in quebec pig farms which had experienced problems of mild to severe respiratory problems. of the 350 sera collected from adults pigs, 67.0%, 18.3% and 46.6% had antibody titers to haemophilus pleuropneumoniae type 1 and/or 5, to porcine adenovirus type 4 and to swine influenza virus, respectively. comparatively, the serological prevalence rates for haemophilus pleuropneumoniae, porcine adenovirus ... | 1985 | 17422542 |
| porcine adenovirus type 3 e1b large protein downregulates the induction of il-8. | replication-defective (e1-e3 deleted) adenovirus vector based gene delivery results in the induction of cytokines including il-8, which may contribute to the development of inflammatory immune responses. like other adenoviruses, e1 + e3 deleted porcine adenovirus (padv) 3 induces the production of il-8 in infected cells. in contrast, no il-8 production could be detected in cells infected with wild-type or mutant padv-3s containing deletion in e1a + e3 (pav211) or e1bsmall + e3 (pav212). expressi ... | 2007 | 17565687 |
| packaging of viral rnas in virions of adenoviruses. | earlier, we detected viral rnas packaged in the porcine adenovirus (padv) -3 virions. using southern blot analysis, we further demonstrated that the viral rnas were predominantly packaged in cscl purified mature capsids (containing viral genome) than empty/intermediate capsids. some of the packaged viral rnas appear to be polyadenylated. real-time reverse transcription (rt)-pcr analysis indicated that the copy number of the tested viral mrnas encoding e1bsmall and fiber proteins was less than on ... | 2009 | 19196470 |
| comparative analysis of vector biodistribution, persistence and gene expression following intravenous delivery of bovine, porcine and human adenoviral vectors in a mouse model. | nonhuman adenoviruses including bovine adenovirus serotype 3 (bad3) and porcine adenovirus serotype 3 (pad3) can circumvent pre-existing immunity against human adenovirus serotype 5 (had5) and are being developed as alternative vectors for gene delivery. to assess the usefulness of these vectors for in vivo gene delivery, we compared biodistribution, persistence, state of vector genome, and transgene and vector gene expression by replication-defective bad3 and pad3 vectors with those of had5 vec ... | 2009 | 19211122 |
| development of a qpcr assay for the quantification of porcine adenoviruses as an mst tool for swine fecal contamination in the environment. | the adenoviridae family comprises a wide diversity of viruses that may be excreted for long periods in feces or urine. previous studies have suggested that the detection of human and animal adenoviruses as well as human and animal polyomaviruses by pcr could be used as an index of fecal contamination of human and animal origin. in this study, quantitative pcr assays targeting specifically porcine adenoviruses have been developed and applied to fecal and environmental samples, including pig slurr ... | 2009 | 19428581 |
| bovine adenovirus serotype 3 utilizes sialic acid as a cellular receptor for virus entry. | bovine adenovirus serotype 3 (bad3) and porcine adenovirus serotype 3 (pad3) entry into the host cells is independent of coxsackievirus adenovirus receptor and integrins. the role of sialic acid in bad3 and pad3 entry was investigated. removal of sialic acid by neuraminidase, or blocking sialic acid by wheat germ agglutinin lectin significantly inhibited bad3, but not pad3, transduction of madin-darby bovine kidney cells. maackia amurensis agglutinin or sambucus nigra (elder) agglutinin treatmen ... | 2009 | 19646729 |
| crystallization of the head and galectin-like domains of porcine adenovirus isolate nadc-1 fibre. | the porcine adenovirus nadc-1 isolate, a strain of porcine adenovirus type 4, has a fibre with an atypical architecture. in addition to a classical virus attachment region, shaft and head domains, it contains an additional galectin like domain c-terminal to the head domain and connected to the head domain by a long rgd-containing loop. the galectin-like domain contains two putative carbohydrate-recognition domains. the head and galectin-like domains have been independently crystallized. diffract ... | 2009 | 19923738 |
| viral multiplex quantitative pcr assays for tracking sources of fecal contamination. | human and animal fecal pollution of the environment presents a risk to human health because of the presence of pathogenic viruses and bacteria. to distinguish between human and animal sources of pollution, we designed specific real-time reverse transcription (rt)-pcr assays for human and animal enteric viruses, including norovirus genogroups i, ii, and iii; porcine adenovirus types 3 and 5; ovine adenovirus; atadenovirus; and human adenovirus species c and f, which are excreted by infected human ... | 2010 | 20061455 |
| evaluation of cross-reactive cell-mediated immune responses among human, bovine and porcine adenoviruses. | the absence of preexisting immunity against porcine adenovirus (ad) serotype 3 (pad3) and bovine ad serotype 3 (bad3) in humans makes them attractive alternatives to human ad serotype 5 (had5) vectors. to determine whether there is significant cross-reactivity among had5, bad3 and pad3 at the level of cell-mediated immune responses, balb/c mice were inoculated intraperitoneally with wild-type (wt) or replication-defective (rd) had5, bad3 or pad3. after 35 days of the first inoculation, cross-rea ... | 2010 | 20164856 |
| crystallographic structure of porcine adenovirus type 4 fiber head and galectin domains. | adenovirus isolate nadc-1, a strain of porcine adenovirus type 4, has a fiber containing an n-terminal virus attachment region, shaft and head domains, and a c-terminal galectin domain connected to the head by an rgd-containing sequence. the crystal structure of the head domain is similar to previously solved adenovirus fiber head domains, but specific residues for binding the coxsackievirus and adenovirus receptor (car), cd46, or sialic acid are not conserved. the structure of the galectin doma ... | 2010 | 20686025 |
| a porcine adenovirus with low human seroprevalence is a promising alternative vaccine vector to human adenovirus 5 in an h5n1 virus disease model. | human adenovirus 5 (adhu5) vectors are robust vaccine platforms however the presence of naturally-acquired neutralizing antibodies may reduce vector efficacy and potential for re-administration. this study evaluates immune responses and protection following vaccination with a replication-incompetent porcine adenovirus 3 (pav3) vector as an alternative vaccine to adhu5 using an avian influenza h5n1 disease model. vaccine efficacy was evaluated in balb/c mice following vaccination with different d ... | 2010 | 21179494 |
| design and application of nucleic acid standards for quantitative detection of enteric viruses by real-time pcr. | synthetic multiple-target rna and dna oligonucleotides were constructed for use as quantification standards for nucleic acid amplification assays for human norovirus genogroup i and ii, hepatitis e virus, murine norovirus, human adenovirus, porcine adenovirus and bovine polyomavirus. this approach overcomes the problems related to the difficulty of obtaining practical quantities of viral rna and dna from these viruses. the quantification capacity of assays using the standards was excellent in ea ... | 2011 | 21765877 |
| detection of circoviruses and porcine adenoviruses in water samples collected from swine manure treatment systems. | samples collected from two swine manure treatment systems including: swine manure treatment system and demonstrative unit (smts and du), were analyzed by qpcr to quantify the amount of porcine adenovirus (padv) and porcine circovirus (pcv2) present. positive samples were tested for virus integrity using dnase assay. fifty-six water samples were collected monthly from march 2009 to may 2010. padv genome was found 66% of the samples in the smts and in 78% of the samples in the du system. pcv2 was ... | 2011 | 21872287 |
| genes indicative of zoonotic and swine pathogens are persistent in stream water and sediment following a swine manure spill. | manure spills into streams are relatively frequent, but no studies have characterized stream contamination with zoonotic and veterinary pathogens, or fecal chemicals, following a spill. we tested stream water and sediment over 25 days and downstream for 7.6 km for the following: fecal indicator bacteria (fib), the fecal indicator chemicals cholesterol and coprostanol, 20 genes for zoonotic and swine-specific bacterial pathogens by presence/absence pcr for viable cells, one swine-specific escheri ... | 2015 | 25769829 |
| hepatitis e virus in pork production chain in czech republic, italy, and spain, 2010. | we evaluated the prevalence of hepatitis e virus (hev) in the pork production chain in czech republic, italy, and spain during 2010. a total of 337 fecal, liver, and meat samples from animals at slaughterhouses were tested for hev by real-time quantitative pcr. overall, hev was higher in italy (53%) and spain (39%) than in czech republic (7.5%). hev was detected most frequently in feces in italy (41%) and spain (39%) and in liver (5%) and meat (2.5%) in czech republic. of 313 sausages sampled at ... | 0 | 22840221 |
| a novel strain of porcine adenovirus detected in urinary bladder urothelial cell culture. | contamination of cell cultures is the most common problem encountered in cell culture laboratories. besides the secondary cell contaminations often occurring in the cell laboratories, the contaminations originating from donor animal or human tissue are equally as common, but usually harder to recognize and as such require special attention. the present study describes the detection of porcine adenovirus (padv), strain padv-svn1 in cultures of normal porcine urothelial (npu) cells isolated from u ... | 2014 | 24960273 |
| presence of human and animal viruses in surface waters in vojvodina province of serbia. | for the first time in serbia, a small surveillance study was conducted in order to estimate the presence and frequency of occurrence of selected human [adenoviruses (hadv), noroviruses, (nov gi, nov gii) and hepatitis a virus (hav)], animal [porcine adenovirus (padv) and bovine polyomavirus (bpyv)] and zoonotic [hepatitis e virus (hev)] viruses in selected surface waters. in total, 60 surface water samples were collected in two sampling occasions at 30 locations, with each sampling time being se ... | 2015 | 25687987 |
| detection of hepatitis e virus in pork liver sausages. | hepatitis e infection is regarded as an emerging public-health concern. the disease is normally self-limiting (mortality rate 1%), but chronic infections have recently been observed in transplanted patients. the etiological agent hev is a small rna virus infecting both humans and animals. in humans, the disease may be food-borne and pig is a main reservoir for zoonotic strains. in the present study, we evaluated the presence of hev and swine fecal cross-contamination in pork liver sausages sold ... | 2015 | 25462920 |
| tracing enteric viruses in the european berry fruit supply chain. | in recent years, numerous foodborne outbreaks due to consumption of berry fruit contaminated by human enteric viruses have been reported. this european multinational study investigated possible contamination routes by monitoring the entire food chain for a panel of human and animal enteric viruses. a total of 785 samples were collected throughout the food production chain of four european countries (czech republic, finland, poland and serbia) during two growing seasons. samples were taken during ... | 2013 | 24135674 |
| high occurrence of hepatitis e virus in samples from wastewater treatment plants in switzerland and comparison with other enteric viruses. | hepatitis e virus (hev) is responsible for many enterically transmitted viral hepatitides around the world. it is currently one of the waterborne diseases of global concern. in industrialized countries, hev appears to be more common than previously thought, even if it is rarely virulent. in switzerland, seroprevalence studies revealed that hev is endemic, but no information was available on its environmental spread. the aim of this study was to investigate -using qpcr- the occurrence and concent ... | 2013 | 23866141 |
| propidium monoazide coupled with pcr predicts infectivity of enteric viruses in swine manure and biofertilized soil. | the use of propidium monoazide (pma) coupled with real-time pcr (rt-qpcr or qpcr for rna or dna viruses, respectively) was assessed to discriminate infectious enteric viruses in swine raw manure, swine effluent from anaerobic biodigester (ab) and biofertilized soils. those samples were spiked either with infectious and heat-inactivated human adenovirus-2 (hadv-2) or mengovirus (vmc0), and pma-qpcr/rt-qpcr allowed discriminating inactivated viruses from the infective particles, with significant r ... | 2016 | 26742766 |
| a novel and simple method for rapid generation of recombinant porcine adenoviral vectors for transgene expression. | many human (different serotypes) and nonhuman adenovirus vectors are being used for gene delivery. however, the current system for isolating recombinant adenoviral vectors is either time-consuming or expensive, especially for the generation of recombinant non-human adenoviral vectors. we herein report a new and simple cloning approach for the rapid generation of a porcine adenovirus (padv-3) vector which shows promise for gene transfer to human cells and evasion of human adenovirus type 5 (hadv- ... | 2015 | 26011074 |
| f-coliphages, porcine adenovirus and porcine teschovirus as potential indicator viruses of fecal contamination for pork carcass processing. | there are concerns about the zoonotic transmission of viruses through undercooked pork products. there is a lack of information on suitable indicator viruses for fecal contamination with pathogenic enteric viruses in the meat processing chain. the study compared the incidence and levels of contamination of hog carcasses with f-coliphages, porcine teschovirus (ptv), and porcine adenovirus (padv) at different stages of the dressing process to assess their potential as indicator viruses of fecal co ... | 2017 | 27810445 |