| microbial transformations of steroids--iv. 6,7-dehydrogenation; a new class of fungal steroid transformation product. | microbial steroid dehydrogenation is quite common. the reaction seems to occur mainly in bacteria and usually results in hydrogen abstraction from positions c(1)-c(2) and/or c(4)-c(5) with occasional aromatisation of ring a. we have screened large numbers of fungal cultures for their ability to monohydroxylate steroids at unusual sites and in the course of our investigations we have identified seven fungal strains capable of dehydrogenating ring b of progesterone and androstenedione at positions ... | 1989 | 2770300 |
| the production of aphidicolin by nigrospora sphaerica. | | 1974 | 4822057 |
| activity against plant pathogenic fungi of phomalactone isolated from nigrospora sphaerica. | phomalactone, 5,6-dihydro-5-hydroxy-6-prop-2-enyl-2h-pyran-2-one, produced by the fungus nigrospora sphaerica, was tested in vitro against nine plant pathogenic fungi, and specifically inhibited the mycelial growth of phytophthora infestans, with an mic value of 2.5 mg litre-1. its inhibitory activities against sporangium and zoospore germination of p infestans were similar to those against phytophthora capsici. in vivo, at 100 and 500 mg litre-1, it reduced the development of tomato late blight ... | 2001 | 11407033 |
| growth factor requirements of six fungi associated with fruit decay. | esposito, r. g. (united fruit co., norwood, mass.), h. greenwood, and a. m. fletcher. growth factor requirements of six fungi associated with fruit decay. j. bacteriol. 83:-250-255. 1962.-a study of the growth factor requirements of representatives of six genera of fruit-rotting fungi has been made. gloeosporium musarum, nigrospora sphaerica, thielaviopsis paradoxa, and verticillium theobromae all required biotin for growth. under certain conditions in nitrate medium, fusarium roseum appeared to ... | 1962 | 16561927 |
| endophytic fungi found in association with smallanthus sonchifolius (asteraceae) as resourceful producers of cytotoxic bioactive natural products. | smallanthus sonchifolius is a traditional andean plant which has been cultured mainly in brazil, japan and new zealand due to its medicinal properties. a study of the endophytic fungi associated to the plant was carried out in order to characterize new cytotoxic agents. thirty two fungal strains were isolated and submitted to cultivation and extraction producing 186 extracts. of these, 12% displayed moderate to high cytotoxic activities and were considered promising anticancer compound sources. ... | 2009 | 18798172 |
| onychomycosis caused by nigrospora sphaerica in an immunocompetent man. | | 2009 | 19451523 |
| two new compounds from the marine nigrospora sphaerica. | two new compounds, 1-(5-oxotetrahydrofuran-2-yl)ethyl 2-phenylacetate (1) and 3-hydroxybutan-2-yl 2-hydroxy-3-phenylpropanoate (2), along with three known compounds, harzialactone a (3), benzeneethanol 4-hydroxy-1-acetate (4), and 1,4-dioxane-2,5-dione-3,6-bis(penylmethyl)-homopolymer (5), have been isolated from the fungus nigrospora sphaerica. their structures were determined on the basis of chemical and spectroscopic methods. | 2009 | 20183261 |
| lc-ms-ms determination of ibuprofen, 2-hydroxyibuprofen enantiomers, and carboxyibuprofen stereoisomers for application in biotransformation studies employing endophytic fungi. | the purpose of this study was the development and validation of an lc-ms-ms method for simultaneous analysis of ibuprofen (ibp), 2-hydroxyibuprofen (2-oh-ibp) enantiomers, and carboxyibuprofen (cooh-ibp) stereoisomers in fungi culture medium, to investigate the ability of some endophytic fungi to biotransform the chiral drug ibp into its metabolites. resolution of ibp and the stereoisomers of its main metabolites was achieved by use of a chiralpak as-h column (150 × 4.6 mm, 5 μm particle size), ... | 2010 | 21079932 |
| assessment of the stereoselective fungal biotransformation of albendazole and its analysis by hplc in polar organic mode. | a high-performance liquid chromatographic method using polar organic mode was developed to analyze albendazole (abz), albendazole sulfone (abzso(2)) and the chiral and active metabolite albendazole sulfoxide (abzsox, ricobendazole) that was further applied in stereoselective fungal biotransformation studies. the chromatographic separation was performed on a chiralpak as column using acetonitrile:ethanol (97:3, v/v) plus 0.2% triethylamine and 0.2% acetic acid as the mobile phase at a flow rate o ... | 2012 | 22230802 |
| a pair of new polyketide enantiomers from three endolichenic fungal strains nigrospora sphaerica, alternaria alternata, and phialophora sp. | a pair of new enantiomeric polyketides, (-)- and (+)-nigrosporaol a (1a and 1b), along with one related known compound, (+)-alternarienoic acid (2), were isolated from three endolichenic fungal strains, nigrospora sphaerica (no.83-1-1-2), alternaria alternata (no.58-8-4-1), and phialophora sp.(no.96-1-8-1). their structures, including the absolute configurations, were elucidated on the basis of spectroscopic methods, x-ray diffraction analysis, and the modified mosher's method. | 2016 | 27534128 |
| heptaketides with antiviral activity from three endolichenic fungal strains nigrospora sp., alternaria sp. and phialophora sp. | two new heptaketides, (+)-(2s,3s,4as)-altenuene (1a) and (-)-(2s,3s,4ar)-isoaltenuene (2a), together with six known compounds, (-)-(2r,3r,4ar)-altenuene (1b), (+)-(2r,3r,4as)-isoaltenuene (2b), 5'-methoxy-6-methyl-biphenyl-3,4,3'-triol (3), alternariol (4), alternariol-9-methyl ether (5), and 4-hydroxyalternariol-9-methyl ether (6) were isolated from the etoac extract of an endolichenic fungal strain nigrospora sphaerica (no.83-1-1-2). compounds 1a and 1b were separated from enantiomers 1 by chi ... | 2012 | 22613072 |
| synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi. | an antifungal dispersion system was prepared by oleoyl-chitosan (o-chitosan) nanoparticles, and the antifungal activity against several plant pathogenic fungi was investigated. under scanning electron microscopy, the nanoparticles formulation appeared to be uniform with almost spherical shape. the particle size of nanoparticles was around 296.962 nm. transmission electron microscopy observation showed that nanoparticles could be well distributed in potato dextrose agar medium. mycelium growth ex ... | 2016 | 26434526 |
| a mixed culture of endophytic fungi increases production of antifungal polyketides. | secondary metabolites produced by endophytic microorganisms can provide benefits to host plants, such as stimulating growth and enhancing the plant's resistance toward biotic and abiotic factors. during its life, a host plant may be inhabited by many species of endophytes within a restrictive environment. this condition can stimulate secondary metabolite production that improves microbial competition and may consequently affect both the neighboring microorganisms and the host plant. the interact ... | 2013 | 24114180 |
| nigrosphaerin a a new isochromene derivative from the endophytic fungus nigrospora sphaerica. | nigrosphaerin a, a new isochromene derivative (1), was isolated from the endophytic fungus nigrospora sphaerica and chemically identified as 3-(3,4-dihydroxyphenyl)-4,6,8-trihydroxy-1h-isochromen-1-one-6-o-β-d-glucopyranoside. in addition nineteen known compounds (2-20) were isolated from the same fungus and chemically identified. compounds (1-3, 5, and 7-16) were isolated for the first time from this fungus. in vitro antileukemic, antileishmanial, antifungal, antibacterial and antimalarial acti ... | 2014 | 27708743 |
| mycosynthesis and characterization of silver nanoparticles and their activity against some human pathogenic bacteria. | the aim of this study was to biosynthesis silver nanoparticles from the fungus nigrospora sphaerica isolated from soil samples and to examine their activity against five human pathogenic strains of bacteria viz. escherichia coli, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi and staphylococcus aureus using disc diffusion method. the synergistic effect of silver nanoparticles in combination with commonly used antibiotic gentamycin against the selected bacteria was also examined. the ... | 2014 | 24627178 |
| endophytic filamentous fungi from a catharanthus roseus: identification and its hydrolytic enzymes. | this paper reported on the various filamentous fungi strains that were isolated from a wild grown catharanthus roseus. based on the morphological characteristics and molecular technique through a polymerase chain reaction and dna sequencing method using internal transcribed spacer (its), these fungi had been identified as a colletotrichum sp., macrophomina phaseolina, nigrospora sphaerica and fusarium solani. the ultrastructures of spores and hyphae were observed under a scanning electron micros ... | 2016 | 27275114 |
| banana peel culture as an indigenous medium for easy identification of late-sporulation human fungal pathogens. | fungi are increasing in incidence as human pathogens and newer and rarer species are continuously being encountered. identifying these species from growth on regular culture media may be challenging due to the absence of typical features. an indigenous and cheap medium, similar to the natural substrate of these fungi, was standardised in our laboratory as an aid to species identification in a conventional laboratory setting. | 2017 | 27934823 |
| importance of producing economic compounds to combat cancer. | the manuscript published by microb biotechnol, volume 10, highlights the relevance of the fungus nigrospora sphaerica, an endophyte isolated from catharanthus roseus, as an alternative source to obtain vinblastine, a compound used in chemotherapy schemes to treat several types of cancer. authors showed that purification of vinblastine from extracts of the fungus has higher activity and yield in comparison with that obtained from the plant catharanthus roseus. this work represents a biotechnologi ... | 2017 | 28127869 |
| phomalactone from a phytopathogenic fungus infecting zinnia elegans (asteraceae) leaves. | zinnia elegans jacq. plants are infected by a fungus that causes dark red spots with necrosis on leaves, particularly in late spring to the middle of summer in the mid-south of the united states. this fungal disease causes the leaves to wilt and eventually kills the plant. the fungus was isolated, cultured in potato dextrose broth, and identified as nigrospora sphaerica by molecular techniques. two major lactone metabolites (phomalactone and catenioblin a) were isolated from liquid culture of n. ... | 2015 | 26133676 |
| antibacterial activity of endophytic fungi from leaves of indigofera suffruticosa miller (fabaceae). | endophytic fungi were isolated from healthy leaves of indigofera suffruticosa miller, a medicinal plant found in brazil which is used in folk medicine to treat various diseases. among 65 endophytic fungi isolated, 18 fungi showed activity against at least one tested microorganism in preliminary screening, and the best results were obtained with nigrospora sphaerica (urm-6060) and pestalotiopsis maculans (urm-6061). after fermentation in liquid media and in semisolid media, only n. sphaerica demo ... | 2015 | 25999918 |
| two new secondary metabolites from the marine-derived fungus nigrospora sphaerica. | two new secondary metabolites, (2s)-5-acetamidopentyl-2-hydroxypropanoate (1) and 2, 5, 7-trihydroxy-4-(3'-methylbut-2'-en-1'-yl) oxy-2h-naphtho [1, 8-b c] furan-9-one (2) were isolated from the marine-derived fungus nigrospora sphaerica. the structures were established on the basis of their spectroscopic data, including 1d nmr and 2d nmr, hr-tof-ms, and the absolute configuration of compound 1 was determined by the mosher method. | 2015 | 25798885 |
| the anti-promyelocytic leukemia mode of action of two endophytic secondary metabolites unveiled by a proteomic approach. | as a result of a program to find antitumor compounds of endophytes from medicinal asteraceae, the steroid (22e,24r)-8,14-epoxyergosta-4,22-diene-3,6-dione (a) and the diterpene aphidicolin (b) were isolated from the filamentous fungi papulaspora immersa and nigrospora sphaerica, respectively, and exhibited strong cytotoxicity against hl-60 cells. a proteomic approach was used in an attempt to identify the drugs' molecular targets and their respective antiproliferative mode of action. results sug ... | 2014 | 24710897 |
| laboratory study of fungal bioreceptivity of different fractions of composite flooring tiles showing efflorescence. | fungi can grow in extreme habitats, such as natural stone and mineral building materials, sometimes causing deterioration. efflorescence-concentrated salt deposits-results from water movement through building material; it can damage masonry materials and other bricks. fungal isolate kur1, capable of growth on, and dissolution of stone chips composing terrazzo-type floor tiles, was isolated from such tiles showing fiber-like crystalline efflorescence. the isolate's ribosomal dna sequences were 10 ... | 2014 | 24652060 |