| phylogenetic position of the diadromus pulchellus ascovirus dna polymerase among viruses with large double-stranded dna genomes. | the ascoviridae: is a family of large double-stranded (ds) dna insect viruses that contains four species, the spodoptera frugiperda (sfav1), trichoplusia ni (tnav2), heliothis virescens (hvav3) and diadromus pulchellus (dpav4) ascoviruses. these are unique among insect viruses in that the primary means of transmission among their lepidopteran hosts is generally by being vectored mechanically by hymenopteran parasitoids. ascoviruses are similar in virion structure, but their relationships with th ... | 2000 | 11086137 |
| characterization of repetitive dna regions and methylated dna in ascovirus genomes. | the accompanying phylogenetic study of large double-stranded dna viruses based on their delta dna polymerase genes suggests that ascoviruses (family ascoviridae:) and iridoviruses (family iridoviridae:) are closely related and may share a common ancestor. this relationship was unexpected because of marked differences between these viruses. iridoviruses produce icosahedral virions and occur broadly among vertebrates and invertebrates, whereas ascoviruses typically produce reniform or bacilliform ... | 2000 | 11086138 |
| evidence for the evolution of ascoviruses from iridoviruses. | ascoviruses (family ascoviridae) are large, enveloped, double-stranded (ds)dna viruses that attack lepidopteran larvae and pupae, and are unusual in that they are transmitted by parasitic wasps during oviposition. previous comparisons of dna polymerase sequences from vertebrate and invertebrate viruses suggested that ascoviruses are closely related to iridoviruses. this relationship was unexpected because these viruses differ markedly in virion symmetry, genome configuration and cellular patholo ... | 2003 | 14573805 |
| sequence and organization of the trichoplusia ni ascovirus 2c (ascoviridae) genome. | the complete trichoplusia ni ascovirus 2c (tnav-2c) genome sequence was determined. the circular genome contains 174,059 bp with 165 open reading frames (orfs) of greater than 180 bp and two major homologous regions (hrs). the genome is quite a+t rich at 64.6%. fifty-four orfs had homologues in other insect viruses, such as ascoviruses, iridoviruses, baculoviruses and entomopoxviruses; 30 orfs showed low identities with those from different parasitic protozoa and 12 orfs were unique to tnav-2c. ... | 2006 | 16876847 |
| genomic sequence of spodoptera frugiperda ascovirus 1a, an enveloped, double-stranded dna insect virus that manipulates apoptosis for viral reproduction. | ascoviruses (family ascoviridae) are double-stranded dna viruses with circular genomes that attack lepidopterans, where they produce large, enveloped virions, 150 by 400 nm, and cause a chronic, fatal disease with a cytopathology resembling that of apoptosis. after infection, host cell dna is degraded, the nucleus fragments, and the cell then cleaves into large virion-containing vesicles. these vesicles and virions circulate in the hemolymph, where they are acquired by parasitic wasps during ovi ... | 2006 | 16987980 |
| p64, a novel major virion dna-binding protein potentially involved in condensing the spodoptera frugiperda ascovirus 1a genome. | we recently identified 21 structural proteins in the virion of spodoptera frugiperda ascovirus 1a (sfav1a), a virus with a large, double-stranded dna genome of 157 kbp, which attacks species of the lepidopteran family noctuidae. the two most abundant virion proteins were the major capsid protein and a novel protein (p64) of 64 kda that contained two distinct domains not known previously to occur together. the amino-terminal half of p64 (residues 1 to 263) contained four repeats (a recently recog ... | 2009 | 19129454 |
| proteomic analysis of the spodoptera frugiperda ascovirus 1a virion reveals 21 proteins. | the spodoptera frugiperda ascovirus 1a (sfav-1a) is a double-stranded dna virus that attacks lepidopteran larvae, in which it produces enveloped virions with complex symmetry which have an average diameter of 130 nm and length of 400 nm. here, we report identification of 21 sfav-1a-encoded proteins that occur in the virion, as determined by nano-liquid chromatography/tandem mass spectrometry. these included a helicase (orf009), nuclease (orf075), atpase (orf047), serine/threonine-like protein ki ... | 2009 | 19141444 |
| ascoviruses: superb manipulators of apoptosis for viral replication and transmission. | ascoviruses are members of a recently described new family (ascoviridae) of large double-stranded dna viruses that attack immature stages of insects belonging to the order lepidoptera, in which they cause a chronic, fatal disease. ascoviruses have several unusual characteristics not found among other viruses, the most novel of which are their transmission by endoparasitic wasps and a unique cytopathology that resembles apoptosis. cell infection induces apoptosis and in some species is associated ... | 2009 | 19216438 |
| symbiotic virus at the evolutionary intersection of three types of large dna viruses; iridoviruses, ascoviruses, and ichnoviruses. | the ascovirus, dpav4a (family ascoviridae), is a symbiotic virus that markedly increases the fitness of its vector, the parasitic ichneumonid wasp, diadromus puchellus, by increasing survival of wasp eggs and larvae in their lepidopteran host, acrolepiopsis assectella. previous phylogenetic studies have indicated that dpav4a is related to the pathogenic ascoviruses, such as the spodoptera frugiperda ascovirus 1a (sfav1a) and the lepidopteran iridovirus (family iridoviridae), chilo iridescent vir ... | 2009 | 19636425 |
| genome analysis of heliothis virescens ascovirus 3h isolated from china. | no ascovirus isolated from china has been sequenced so far. therefore, in this study, we aimed to sequence the genome of heliothis virescens ascovirus 3h (hvav-3h) using the 454 pyrosequencing technology. the genome was found to be 190,519-bp long with a g+c content of 45.5%. we also found that it encodes 185 hypothetical open reading frames (orfs) along with at least 50 amino acids, including 181 orfs found in other ascoviruses and 4 unique orfs. gene-parity plots and phylogenetic analysis reve ... | 2017 | 28382574 |