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[on the taxonomy of aeromonas salmonicida subsp. achromogenes (smith 1963) schubert 1967 and aeromonas salmonicida subsp. masoucida kimura 1969]. 19695386249
enterotoxigenicity of aeromonas strains in suckling mice.the enterotoxigenicity of 170 aeromonas strains isolated from different sources (food poisoning, random food sampling, water, faeces) was examined by the suckling mouse test. the strains were grown on syncaye culture medium covered with sterilized membrane for kiil-kidney. the culture supernatants were inoculated orally. ileal loop dilatation was compared to that produced by the international standard enterotoxic escherichia coli b7a (o148 : h28) and b2c (o6 : h16) strains. of the 87 aeromonas h ...19807415872
three beta-lactamases isolated from aeromonas salmonicida, including a carbapenemase not detectable by conventional methods.the beta-lactamases of seven strains of aeromonas salmonicida subsp. achromogenes resistant to amoxicillin (mic > 1024 mg/l) and responsible for furunculosis in farmed atlantic salmon in scotland were examined to establish the mechanisms of beta-lactam resistance. separation of a cell-free extract on an isoelectric focusing gel stained with the chromogenic cephalosporin nitrocefin showed the presence of two beta-lactamases, one with a pi of 7.9 and the other with a pi of 6.0. hydrolysis assays o ...19947889949
experimental infection of turbot, scophthalmus maximus (l.), by aeromonas salmonicida subsp. achromogenes and evaluation of cross protection induced by a furunculosis vaccine.turbot was shown to be sensitive to injection challenges by aeromonas salmonicida subsp. achromogenes (asa). a systemic disease was induced and the bacterium was isolated from various internal organs. histopathological changes involved haemorrhages, necrosis and degeneration in skin and muscle, haemorrhages and necrosis in kidney, degeneration in the heart muscle, and fusion of the secondary gill lamellae. a polyvalent commercial salmon vaccine, containing a. salmonicida subsp. salmonicida as on ...200515752279
isolation of putative probionts from cod rearing environment.survival problems are encountered at early stages of intensive fish rearing and antibiotics are widely used to remedy the situation. probiotics may provide a potential alternative method to protect larvae from opportunistic and pathogenic bacteria and promote a balanced environment. this study was designed to search for new probiotics to target this critical period in cod rearing. potential probionts were selected from the natural microbiota of cod aquacultural environment. the selection was bas ...200818599226
the asap1 peptidase of aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family m35) and a major virulence factor.infections by the bacterium aeromonas salmonicida subsp. achromogenes cause significant disease in a number of fish species. in this study, we showed that asap1, a toxic 19-kda metallopeptidase produced by a. salmonicida subsp. achromogenes, belongs to the group of extracellular peptidases (aeromonas type) (merops id m35.003) of the deuterolysin family of zinc-dependent aspzincin endopeptidases. the structural gene of asap1 was sequenced and found to be highly conserved among gram-negative bacte ...200918952802
crystallization and preliminary x-ray diffraction studies of asap1_e294a and asap1_e294q, two inactive mutants of the toxic zinc metallopeptidase asap1 from aeromonas salmonicida subsp. achromogenes.two mutants of the toxic extracellular zinc endopeptidase asap1 (asap1_e294q and asap1_e294a) of aeromonas salmonicida subsp. achromogenes were expressed in escherichia coli and crystallized by the vapour-diffusion method. crystals were obtained using several precipitants and different protein concentrations. protein crystals were found in a monoclinic (c2) as well as an orthorhombic (p2(1)2(1)2(1)) space group. the crystals belonging to the monoclinic space group c2 had unit-cell parameters a = ...200919574642
quorum sensing in aeromonas salmonicida subsp. achromogenes and the effect of the autoinducer synthase asai on bacterial virulence.the gram-negative fish pathogenic bacterium aeromonas salmonicida possesses the luxir-type quorum sensing (qs) system, termed asair. in this study the role of qs in a. salmonicida subsp. achromogenes virulence and pigment production was investigated. five wild-type asa strains induced the n-acyl-homoserinelactone (ahl) monitor bacteria. hplc-hr-ms analysis identified only one type of ahl, n-butanoyl-l-homoserine lactone (c4-hsl). a knock out mutant of asai, constructed by allelic exchange, did n ...201120708354
construction of aeromonas salmonicida subsp. achromogenes asap1-toxoid strains and study of their ability to induce immunity in arctic char, salvelinus alpinus l.the metalloendopeptidase asap1 is one of the major extracellular virulence factors of a. salmonicida subsp. achromogenes, expressed as a 37-kda pre-pro-peptide and processed to a 19-kda active peptide. the aim of this study was to construct mutant strains secreting an asap1-toxoid instead of asap1-wt, to study virulence of these strains and to test the potency of the asap1-toxoid bacterin and the recombinant asap1-toxoids to induce protective immunity in arctic char. two a. salmonicida mutants w ...201425271952
draft genome sequence of aeromonas salmonicida subsp. achromogenes as03, an atypical strain isolated from crucian carp (carassius carassius) in the republic of korea.we present the draft genome sequence of aeromonas salmonicida subsp. achromogenes strain as03, an atypical a. salmonicida strain that causes erythrodermatitis in crucian carp (carassius carassius). this is the first genome sequence report of a. salmonicida subsp. achromogenes, one of the four subspecies of atypical a. salmonicida.201324092786
innate and adaptive immune responses of arctic charr (salvelinus alpinus, l.) during infection with aeromonas salmonicida subsp. achromogenes and the effect of the asap1 toxin.aeromonas salmonicida subsp. achromogenes, the causative agent of atypical furunculosis in many fish species, secretes the toxic metalloendopeptidase asap1. this study aimed to analyze innate and adaptive immune parameters induced in arctic charr (salvelinus alpinus, l.) infected with wild type (wt) a. salmonicida subsp. achromogenes and its isogenic asap1 deletion mutant (asap1-deficient). head-kidney, liver and spleen were obtained from i.p. infected charr (wt, asap1-deficient), during a time ...201323811350
structural evidence of intramolecular propeptide inhibition of the aspzincin metalloendopeptidase asap1.the gram-negative bacterium aeromonas salmonicida is a fish pathogen for various fish species worldwide. aeromonas salmonicida subsp. achromogenes produces the extracellular, toxic zinc endopeptidase asap1. crystal structure analyses at 2.0 å resolution of two proteolytically inactive asap1 variants show the polypeptide folding of the protease domain and the propeptide domain. these first crystal structure analyses of a precursor of a deuterolysin-like aspzincin protease provide insights into pr ...201627528449
induction of inflammatory cytokines by extracellular products and lps of the fish pathogen aeromonas salmonicida ssp. achromogenes in mice and mouse cell cultures.balb/c mice, injected i.p. with extracellular products (ecp) of aeromonas salmonicida subsp. achromogenes (asa), displayed symptoms similar to toxic shock syndrome. the ld(50) observed was between 1.5 and 2.0 microg g(-1) and the mice died within 19 h. four inflammatory cytokines were measured in mice receiving sublethal ecp doses. tnf-alpha and il-6 showed a sharp peak in the serum while il-1 beta and il-2 were not detected. when peritoneal macrophages were cultivated in the presence of ecp, as ...200111498248
monoclonal antibodies against asap1, a major exotoxin of the fish pathogen aeromonas salmonicida subsp. achromogenes, and their application in elisa.two monoclonal antibodies (mabs) binding to a toxic extracellular metallo-proteinase of aeromonas salmonicida subsp. achromogenes, asap1, were produced. both reacted with common epitopes of the native enzyme and recognized this 20 kda antigen on western blots. one of these mabs had an inhibitory effect on the caseinase activity of the exotoxin. a mab-based elisa was set up and evaluated for serological detection of asap1 in bacterial culture filtrates. the exotoxin was identified serologically i ...199910583691
a comparison of total and specific immunoglobulin levels in healthy atlantic salmon (salmo salar l.) and in salmon naturally infected with aeromonas salmonicida subsp. achromogenes.healthy atlantic salmon and salmon with a history of chronic natural aeromonas salmonicida subsp. achromogenes infection were compared with respect to total serum protein and the concentration and specificity of serum immunoglobulin. the immunoglobulin level was measured using competitive elisa and the specific antibody activity against aeromonas salmonicida subsp. achromogenes was measured using double sandwich elisa. significant elevation of serum protein and immunoglobulin concentration was o ...19921604799
toxoid construction of asap1, a lethal toxic aspzincin metalloendopeptidase of aeromonas salmonicida subsp. achromogenes, and studies of its activity and processing.asap1 is a toxic aspzincin metalloendopeptidase secreted by the fish pathogen aeromonas salmonicida subsp. achromogenes. the protease is highly immunogenic and antibodies against asap1 evoke a passive protection against infection with a. salmonicida subsp. achromogenes. the protease is expressed as 37 kda pre-pro-protein and processed to an active enzyme of 19kda in a. salmonicida subsp. achromogenes. recombinant expression of asap1(rec) in e. coli results in a protease of 22 kda that is not sec ...201323031341
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