| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| coaggregation studies of the eubacterium species. | eubacterium species are gram-positive anaerobic rods that are frequently isolated from subgingival plaque of periodontal pockets. five eubacterium species were tested for their ability to coaggregate with 33 oral bacterial strains. using visual and turbidimetric assays, coaggregation was observed among eubacterium brachy, eubacterium nodatum, eubacterium alactolyticum and eubacterium limosum strains only when tested with fusobacterium nucleatum strains; eubacterium saburreum displayed only weak ... | 1992 | 1494452 |
| reactivity of igg from explant cultures of periapical lesions with implicated microorganisms. | the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ... | 1991 | 1940739 |
| a comparison of the reactivity of eubacterium species with localized and serum immunoglobulins from rapidly progressive and adult periodontitis patients. | immunoglobulins in sera and in supernatant fluids of explant cultures of diseased gingival tissues from 20 rapidly progressive and 20 adult periodontitis sites were tested by an elisa assay for reactivity with typed strains of eubacterium alactolyticum, e. brachy, e. limosum and e. nodatum. immunoglobulins present in tissue culture fluids from both rapidly progressive and adult periodontitis samples reactive with e. brachy and e. nodatum were significantly greater (p less than 0.05) than those r ... | 1988 | 3422290 |
| neutrophil chemotaxis induced by anaerobic bacteria isolated from necrotic dental pulps. | anaerobic bacteria belonging to the species bacteroides melaninogenicus, bacteroides asaccharolyticus, fusobacterium nucleatum, peptostreptococcus anaerobius and eubacterium alactolyticum, isolated from necrotic dental pulps, were tested for their capacity to induce neutrophil leukocyte chemotaxis. generation of chemotactic factors in serum by whole bacterial cells and chemotaxis induced by bacterial extracts were studied in vitro by the boyden technique. subcutaneously implanted wound chambers ... | 1980 | 6103573 |
| bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. | the presence of bacteria in 17 single-rooted teeth, with periapical lesions, was studied throughout a whole period of treatment. the root canals were irrigated with physiologic saline solution during instrumentation. no antibacterial solutions or dressings were used. bacteria were found in all initial specimens from the teeth (median number of bacterial cells 4 x 10(5), range 10(2) - 10(7)) and the number of strains in the specimens ranged from 1 to 10.88% of the strains were anaerobic. the most ... | 1981 | 6947391 |
| profile of subgingival microbiota in children with primary dentition. | eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ... | 2000 | 10791707 |
| profile of subgingival microbiota in children with mixed dentition. | a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ... | 2000 | 11155173 |
| an immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions. | we immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. the bacteria in the specimens were detected by brown-brenn stain and the labeled-streptavidin-biotin method with specific antisera for 16-bacteria. seventy percent of the examined teeth showed bacteria in ... | 2003 | 12669880 |
| [research on the pathogenicity of ramibacterium pleuriticum]. | | 1958 | 13583645 |
| polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. | in this study, we aimed to investigate the occurrence of several microbial species in cases of failed endodontic therapy by means of the polymerase chain reaction (pcr). study design root canal samples were taken from 22 root-filled teeth with persistent periradicular lesions selected for re-treatment. dna was extracted from the samples and analyzed for the presence of 19 microbial taxa by using the polymerase chain reaction. | 2004 | 14716262 |
| cultivable bacteria in infected root canals as identified by 16s rrna gene sequencing. | introduction: traditionally, cultivable bacteria isolated from infected root canals have been identified by phenotype-based methods. because 16s ribosomal rna (rrna) gene sequencing has emerged as a more accurate and reliable tool for bacterial identification, the present study applied this approach to identify bacterial isolates recovered from the root canals of teeth with chronic apical periodontitis. methods: anaerobic techniques were used for culturing; identification of the isolates was car ... | 2007 | 17600539 |
| bacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography. | the aim of this study was to examine the diversity of bacterial species in the infected root canals of teeth associated with endodontic abscesses by cloning and sequencing techniques in concert with denaturing high-performance liquid chromatography. | 2007 | 17949344 |
| bacterial profiles of root caries in elderly patients. | culture-based studies have shown that streptococcus mutans and lactobacilli are associated with root caries (rc). the purpose of the present study was to assess the bacterial diversity of rc in elderly patients by use of culture-independent molecular techniques and to determine the associations of specific bacterial species or bacterial communities with healthy and carious roots. plaque was collected from root surfaces of 10 control subjects with no rc and from 11 subjects with rc. the bacterial ... | 2008 | 18385433 |
| diversity of the resident microbiota in a thermophilic municipal biogas plant. | biogas plants continuously convert biological wastes mainly into a mixture of methane, co2 and h2o-a conversion that is carried out by a consortium of bacteria and archaea. especially in the municipal plants dedicated towards waste treatment, the reactor feed may vary considerably, exposing the resident microbiota to a changing variety of substrates. to evaluate how and if such changes influence the microbiology, an established biogas plant (6,600 m3, up to 600 m3 biogas per h) was followed over ... | 2008 | 18820906 |
| bacteria in the apical root canal of teeth with primary apical periodontitis. | bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. this study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis. | 2009 | 19426923 |
| starvation response and growth in serum of fusobacterium nucleatum, peptostreptococcus anaerobius, prevotella intermedia, and pseudoramibacter alactolyticus. | the microbiota inhabiting the untreated root canal differ markedly from those found in post-treatment disease, yet there is limited information on the microbial characteristics distinguishing the different infections. we hypothesized that starvation survival is a key microbial property in species selection. this study analyzed starvation-survival behavior over 60 days of species representative of the untreated root canal infection: fusobacterium nucleatum, peptostreptococcus anaerobius, prevotel ... | 2009 | 19540450 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| analysis of microbiota associated with peri-implantitis using 16s rrna gene clone library. | peri-implantitis (pi) is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. the subgingival microbiota appears to be responsible for peri-implant lesions and although the complexity of the microbiota has been reported in pi, the microbiota responsible for pi has not been identified. | 2010 | 21523229 |
| new bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings. | the aim of the present study was to analyze the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings. | 2014 | 24767562 |
| isolation of alkaline-tolerant bacteria from primary infected root canals. | alkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. the aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (s1), after chemomechanical root canal preparation (s2), and after calcium hydroxide dressing (s3). | 2015 | 25638530 |
| total and specific bacterial levels in the apical root canal system of teeth with post-treatment apical periodontitis. | most studies of the microbiota in root canal-treated teeth focused only on the main canal, not distinguishing regions nor incorporating the intricate anatomy in the analysis. moreover, most of them provided only prevalence data. this study was designed to evaluate the total bacterial counts and the presence, levels, and relative abundance of candidate endodontic pathogens exclusively in the apical root canal system associated with post-treatment apical periodontitis. | 2015 | 25892512 |
| characterization of microbiota of root canal-treated teeth with posttreatment disease. | this study evaluated the microbiota of root canals undergoing retreatment. the most prevalent taxa detected by checkerboard included propionibacterium species, fusobacterium nucleatum, streptococci, and pseudoramibacter alactolyticus. quantitative real-time pcr detected enterococcus faecalis and streptococci in 38% and 41% of the cases, comprising 9.76% and 65.78% of the total bacterial counts, respectively. the findings call into question the status of e. faecalis as the main pathogen and sugge ... | 2012 | 22403423 |
| advanced caries microbiota in teeth with irreversible pulpitis. | bacterial taxa in the forefront of caries biofilms are candidate pathogens for irreversible pulpitis and are possibly the first ones to invade the pulp and initiate endodontic infection. this study examined the microbiota of the most advanced layers of dentinal caries in teeth with irreversible pulpitis. | 2015 | 26187422 |
| molecular identification of cultivable bacteria from infected root canals associated with acute apical abscess. | the objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16s rrna) of cultivable bacteria. two hundred and twenty strains isolated by culture from 20 root canals were subjected to dna extraction and amplification of the 16s rrna gene (pcr), followed by sequencing. the resulting nucleotide sequences were compared to the genbank database from the national center of biotechnology inform ... | 2017 | 27224567 |
| analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16s rdna gene sequence. | to analyze and characterize the predominant bacterial flora associated with peri-implantitis by using culture techniques under obligate anaerobic conditions and 16s rdna gene sequences. | 2013 | 24278920 |
| impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray. | this study compares the changes to the subgingival microbiota of individuals with "refractory" periodontitis (rp) or treatable periodontitis (good responders [gr]) before and after periodontal therapy by using the human oral microbe identification microarray (homim) analysis. | 2012 | 22324467 |
| exploring bacterial diversity of endodontic microbiota by cloning and sequencing 16s rrna. | the characterization of microbial communities infecting the endodontic system in each clinical condition may help on the establishment of a correct prognosis and distinct strategies of treatment. the purpose of this study was to determine the bacterial diversity in primary endodontic infections by 16s ribosomal-rna (rrna) sequence analysis. | 2011 | 21689545 |
| [protease activity of microflora in the oral cavity of patients with periodontitis]. | microbial spectrum and non-specific as well as specific iga1 protease activity of isolated microorganisms were investigated in gingival liquid of patients with periodontitis. microorganisms from the gingival liqud of these patients belonged to conditional-pathogenic obligate and facultatively anaerobic bacteria. 24 strains of microorganisms have been identified. nonspecific proteolytic activity was found in the following microorganisms: actinomyces israelii, actinomyces naeslundii, aerococcus vi ... | 2008 | 19205430 |
| microarray analysis of the microflora of root caries in elderly. | the present study used a new 16s rrna-based microarray with probes for over 300 bacterial species to better define the bacterial profiles of healthy root surfaces and root caries (rc) in the elderly. supragingival plaque was collected from 20 healthy subjects (controls) and from healthy and carious roots and carious dentin from 21 rc subjects (patients). collectively, 179 bacterial species and species groups were detected. a higher bacterial diversity was observed in controls than in patients. l ... | 2009 | 19039610 |
| molecular analysis of microbial diversity in advanced caries. | real-time pcr analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. this suggests that an unresolved complexity exists in bacteria associated with advanced caries. in this report, the profile of the microflora of carious dentine was explored by using dna extracted from 10 lesions selected on the basis of comparable total microbial load and on the relative abundance of prevotella spp. using universal primers for t ... | 2005 | 15695690 |
| comparison of type 1 and type 2 cytokine production by mononuclear cells cultured with streptococcus mutans and selected other caries bacteria. | a feature of pulpal immune responses is the predominance of type 1 cytokine mrna under shallow caries and a mixed (type 1/type 2) profile under deep caries. these results prompted an examination of the cytokine profiles induced by bacteria in shallow caries (streptococcus mutans and actinomyces viscosus) and deep caries (lactobacillus casei, pseudoramibacter alactolyticus, and prevotella intermedia). all isolates induced interferon-gamma and interleukin-10, whereas interleukin-4 and interleukin- ... | 2004 | 15107645 |
| pseudoramibacter alactolyticus in primary endodontic infections. | a nested polymerase chain reaction (pcr)-based method was used to directly survey samples taken from primary endodontic infections for the occurrence of pseudoramibacter alactolyticus. identification by nested pcr was performed in root-canal samples from teeth associated with asymptomatic periradicular lesions or acute apical periodontitis, and in pus samples from acute periradicular abscesses. dna was extracted from the samples and initially amplified using universal 16s rdna primers. a second ... | 2003 | 14651280 |
| [studies on 25 strains of nonsporular anaerobic bacteria, ramibacterium pleuriticum]. | | 1953 | 13268931 |
| characterisation of eubacterium-like strains isolated from oral infections. | the genus eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. as a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remains indifferent. in this study, 105 isolates from odontogenic infections, infections associated with dental implants or saliva from healthy subjects and provisionally assigned to the ... | 2001 | 11699590 |
| anaerovorax odorimutans gen. nov., sp. nov., a putrescine-fermenting, strictly anaerobic bacterium. | the strictly anaerobic, gram-positive, non-spore-forming bacterium strain norput1t ferments putrescine to acetate, butyrate, molecular hydrogen and ammonia. it also utilizes 4-aminobutyrate and 4-hydroxybutyrate as growth substrates. comparative 16s rdna sequence analysis confirmed a phylogenetic affiliation of this strain to the phylum of gram-positive bacteria with low dna g+c content. together with its closest relative, 'clostridium aminobutyricum' (dsm 2634), and several eubacterium species, ... | 2000 | 10939665 |
| subgingival microflora associated with nifedipine-induced gingival overgrowth. | the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ... | 1998 | 9660335 |
| phylogenetic relationships of the genera acetobacterium and eubacterium sensu stricto and reclassification of eubacterium alactolyticum as pseudoramibacter alactolyticus gen. nov., comb. nov. | 16s rrna gene sequences of the type strains of the seven previously described acetobacterium species were determined. the acetobacterium species were found to form a tight phylogenetic cluster within the clostridium subphylum of the gram-positive bacteria. within this subphylum these organisms belong to cluster xv as defined by collins et al. (m.d. collins, p.a. lawson, a. willems, j.j. cordoba, j. fernandez-garayzabal, p. garcia, j. cai, h. hippe, and j. a. e. farrow, int. j. syst. bacteriol. 4 ... | 1996 | 8863439 |
| chemical composition of eubacterium alactolyticum cell wall peptidoglycan. | the mechanism of lysis of eubacterium alactolyticum cell walls by streptomyces albus g enzyme was studied. the analysis of the peptide terminal groups and peptide subunits isolated from the cell wall digest, released during solubilization of the cell walls, revealed that lytic action of s. albus g enzyme was mainly due to d-alanyl-a2pm endopeptidase, n-acetylmuramyl-l-alanine amidase, n-acetylmuramidase and n-acetylglucosaminidase. e. alactolyticum cell wall peptidoglycan is composed mainly of g ... | 1989 | 2742450 |
| antibodies in normal and diseased pulps reactive with microorganisms isolated from deep caries. | immunoglobulin molecules in the supernatant fluids (sf) from pulpal explant cultures have been observed to react with microorganisms implicated in infections of root canals. in this study, the reactivity of immunoglobulin molecules in the sf from normal and irreversible pulpitis pulps to six strains of predominant microorganisms isolated from the immediate layer of carious lesions above the pulps used for explant cultures was investigated using an enzyme-linked immunosorbent assay. two atcc stra ... | 1992 | 1402561 |
| preservation of bacterial dna by human dentin. | the capacity of dentin and collagen to bind dna and protect against spontaneous and nuclease-induced degradation was evaluated individually and by the incubation of dna with nuclease-producing bacteria in a mixed culture. | 2014 | 24461411 |
| selective persistence of propionibacterium species fma5 following sealing of infected dentinal matrix. | the objective of the present study was to test the hypothesis that nutrient deprivation by effective isolation should inactivate causative saccharolytic bacteria occupying carious lesions. vital maxillary third molar teeth were prepared by removing only the superficial necrotic material, leaving behind infected dentinal matrix, before the cavity was sealed with glass ionomer cement (gic). before sealing, lesions were biopsied to provide reference bacterial dna for microbial analysis. after an in ... | 2016 | 28013413 |
| evaluation of bacteriological profile in the apical root segment of the patients with primary apical periodontitis. | apical periodontitis usually results from bacterial accumulation and contamination occurring in the root-canal system, and extending beyond the apical foramen to involve the periapical tissues. literature has a paucity of the studies that stress on the division and analysis of the pulp canal segments. the reason for this disparity might be the technique used for collecting the samples from the pulp canals. hence, we carried out the present study to evaluate the microbial flora in the apical part ... | 2017 | 28050984 |