| exploring bacterial diversity of endodontic microbiota by cloning and sequencing 16s rrna. | the characterization of microbial communities infecting the endodontic system in each clinical condition may help on the establishment of a correct prognosis and distinct strategies of treatment. the purpose of this study was to determine the bacterial diversity in primary endodontic infections by 16s ribosomal-rna (rrna) sequence analysis. | 2011 | 21689545 |
| analysis of microbiota associated with peri-implantitis using 16s rrna gene clone library. | peri-implantitis (pi) is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. the subgingival microbiota appears to be responsible for peri-implant lesions and although the complexity of the microbiota has been reported in pi, the microbiota responsible for pi has not been identified. | 2010 | 21523229 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| starvation response and growth in serum of fusobacterium nucleatum, peptostreptococcus anaerobius, prevotella intermedia, and pseudoramibacter alactolyticus. | the microbiota inhabiting the untreated root canal differ markedly from those found in post-treatment disease, yet there is limited information on the microbial characteristics distinguishing the different infections. we hypothesized that starvation survival is a key microbial property in species selection. this study analyzed starvation-survival behavior over 60 days of species representative of the untreated root canal infection: fusobacterium nucleatum, peptostreptococcus anaerobius, prevotel ... | 2009 | 19540450 |
| microarray analysis of the microflora of root caries in elderly. | the present study used a new 16s rrna-based microarray with probes for over 300 bacterial species to better define the bacterial profiles of healthy root surfaces and root caries (rc) in the elderly. supragingival plaque was collected from 20 healthy subjects (controls) and from healthy and carious roots and carious dentin from 21 rc subjects (patients). collectively, 179 bacterial species and species groups were detected. a higher bacterial diversity was observed in controls than in patients. l ... | 2009 | 19039610 |
| diversity of the resident microbiota in a thermophilic municipal biogas plant. | biogas plants continuously convert biological wastes mainly into a mixture of methane, co2 and h2o-a conversion that is carried out by a consortium of bacteria and archaea. especially in the municipal plants dedicated towards waste treatment, the reactor feed may vary considerably, exposing the resident microbiota to a changing variety of substrates. to evaluate how and if such changes influence the microbiology, an established biogas plant (6,600 m3, up to 600 m3 biogas per h) was followed over ... | 2008 | 18820906 |
| bacterial profiles of root caries in elderly patients. | culture-based studies have shown that streptococcus mutans and lactobacilli are associated with root caries (rc). the purpose of the present study was to assess the bacterial diversity of rc in elderly patients by use of culture-independent molecular techniques and to determine the associations of specific bacterial species or bacterial communities with healthy and carious roots. plaque was collected from root surfaces of 10 control subjects with no rc and from 11 subjects with rc. the bacterial ... | 2008 | 18385433 |
| bacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography. | the aim of this study was to examine the diversity of bacterial species in the infected root canals of teeth associated with endodontic abscesses by cloning and sequencing techniques in concert with denaturing high-performance liquid chromatography. | 2007 | 17949344 |
| cultivable bacteria in infected root canals as identified by 16s rrna gene sequencing. | introduction: traditionally, cultivable bacteria isolated from infected root canals have been identified by phenotype-based methods. because 16s ribosomal rna (rrna) gene sequencing has emerged as a more accurate and reliable tool for bacterial identification, the present study applied this approach to identify bacterial isolates recovered from the root canals of teeth with chronic apical periodontitis. methods: anaerobic techniques were used for culturing; identification of the isolates was car ... | 2007 | 17600539 |
| anaerofustis stercorihominis gen. nov., sp. nov., from human feces. | phenotypic and phylogenetic studies were performed on an unidentified gram-positive, strictly anaerobic, non-spore-forming, rod-shaped bacterium isolated from human feces. the organism was catalase-negative, resistant to 20% bile, produced acetic and butyric acids as end products of glucose metabolism, and possessed a g+c content of approximately 70 mol%. comparative 16s rrna gene sequencing demonstrated that the unidentified bacterium was a member of the clostridium sub-phylum of the gram-posit ... | 2004 | 16701499 |
| molecular analysis of microbial diversity in advanced caries. | real-time pcr analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. this suggests that an unresolved complexity exists in bacteria associated with advanced caries. in this report, the profile of the microflora of carious dentine was explored by using dna extracted from 10 lesions selected on the basis of comparable total microbial load and on the relative abundance of prevotella spp. using universal primers for t ... | 2005 | 15695690 |
| comparison of type 1 and type 2 cytokine production by mononuclear cells cultured with streptococcus mutans and selected other caries bacteria. | a feature of pulpal immune responses is the predominance of type 1 cytokine mrna under shallow caries and a mixed (type 1/type 2) profile under deep caries. these results prompted an examination of the cytokine profiles induced by bacteria in shallow caries (streptococcus mutans and actinomyces viscosus) and deep caries (lactobacillus casei, pseudoramibacter alactolyticus, and prevotella intermedia). all isolates induced interferon-gamma and interleukin-10, whereas interleukin-4 and interleukin- ... | 2004 | 15107645 |
| polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. | in this study, we aimed to investigate the occurrence of several microbial species in cases of failed endodontic therapy by means of the polymerase chain reaction (pcr). study design root canal samples were taken from 22 root-filled teeth with persistent periradicular lesions selected for re-treatment. dna was extracted from the samples and analyzed for the presence of 19 microbial taxa by using the polymerase chain reaction. | 2004 | 14716262 |
| pseudoramibacter alactolyticus in primary endodontic infections. | a nested polymerase chain reaction (pcr)-based method was used to directly survey samples taken from primary endodontic infections for the occurrence of pseudoramibacter alactolyticus. identification by nested pcr was performed in root-canal samples from teeth associated with asymptomatic periradicular lesions or acute apical periodontitis, and in pus samples from acute periradicular abscesses. dna was extracted from the samples and initially amplified using universal 16s rdna primers. a second ... | 2003 | 14651280 |
| characterisation of eubacterium-like strains isolated from oral infections. | the genus eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. as a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remains indifferent. in this study, 105 isolates from odontogenic infections, infections associated with dental implants or saliva from healthy subjects and provisionally assigned to the ... | 2001 | 11699590 |
| profile of subgingival microbiota in children with mixed dentition. | a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ... | 2000 | 11155173 |
| anaerovorax odorimutans gen. nov., sp. nov., a putrescine-fermenting, strictly anaerobic bacterium. | the strictly anaerobic, gram-positive, non-spore-forming bacterium strain norput1t ferments putrescine to acetate, butyrate, molecular hydrogen and ammonia. it also utilizes 4-aminobutyrate and 4-hydroxybutyrate as growth substrates. comparative 16s rdna sequence analysis confirmed a phylogenetic affiliation of this strain to the phylum of gram-positive bacteria with low dna g+c content. together with its closest relative, 'clostridium aminobutyricum' (dsm 2634), and several eubacterium species, ... | 2000 | 10939665 |
| profile of subgingival microbiota in children with primary dentition. | eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ... | 2000 | 10791707 |
| recently described clinically important anaerobic bacteria: taxonomic aspects and update. | a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ... | 1997 | 9310640 |
| phylogenetic relationships of the genera acetobacterium and eubacterium sensu stricto and reclassification of eubacterium alactolyticum as pseudoramibacter alactolyticus gen. nov., comb. nov. | 16s rrna gene sequences of the type strains of the seven previously described acetobacterium species were determined. the acetobacterium species were found to form a tight phylogenetic cluster within the clostridium subphylum of the gram-positive bacteria. within this subphylum these organisms belong to cluster xv as defined by collins et al. (m.d. collins, p.a. lawson, a. willems, j.j. cordoba, j. fernandez-garayzabal, p. garcia, j. cai, h. hippe, and j. a. e. farrow, int. j. syst. bacteriol. 4 ... | 1996 | 8863439 |
| bacteria in the apical root canal of teeth with primary apical periodontitis. | bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. this study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis. | 2009 | 19426923 |