| inhibitory effects of physalin b and physalin f on various human leukemia cells in vitro. | physalins b and f were isolated and characterized from the ethanolic extract of the whole plant of physalis angulata l. (solanaceae). both physalin b and physalin f inhibited the growth of several human leukemia cells: k562 (erythroleukemia), apm1840 (acute t lymphoid leukemia), hl-60 (acute promyelocytic leukemia), kg-1 (acute myeloid leukemia), ctv1 (acute monocytic leukemia) and b cell (acute b lymphoid leukemia). physalin f showed a stronger activity against these leukemia cells than physali ... | 1992 | 1503404 |
| antitumor agent, physalin f from physalis angulata l. | physalin f and physalin d were isolated and characterized from the ethanolic extract of the whole plant of physalis angulata l. (solanaceae). systematic fractionation of the ethanolic extract of the plant led to characterization of physalin f from the fraction paiv-2 as an active ingredient which showed cytotoxicity in vitro by dea and mtt assays on 8 cancer cell lines, five human cancer cell lines: ha22t(hepatoma), hela(cervix uteri), kb(nasopharynx), colo-205(colon) and calu-1(lung); and three ... | 1992 | 1622143 |
| induction of heat-shock response and alterations of protein phosphorylation by a novel topoisomerase ii inhibitor, withangulatin a, in 9l rat brain tumor cells. | withangulatin a is a newly identified in vitro topoisomerase ii inhibitor isolated from the chinese antitumor herb physalis angulata. in vivo, it was found to be cytotoxic, capable of suppressing general protein synthesis and of inducing the synthesis of a small set of proteins including those generated by heat-shock treatment. the 70 kda protein generated by withangulatin a was unequivocally identified as the heat-shock protein 70 (hsp70) since both proteins migrated to the same position on two ... | 1991 | 1658010 |
| trypanosomatid protozoa in fruit of solanaceae in southeastern brazil. | fruits of cultivated and indigenous solanaceae from southeastern brazil have been examined for the presence of trypanosomatid flagellates. the 14 species found infected were: capsicum annuum, c. praetermissum, lycopersicon esculentum, nicandra physaloides, physalis angulata, solanum sp., s. americanum, s. concinnum, s. diflorum, s. erianthum, s. gilo, s. robustum, s. variable and s. viarum. the pentatomid hemipteran arvelius albopunctatus experimentally transmitted flagellates to fruits of some ... | 1990 | 2152193 |
| a new compound, withangulatin a, promotes type ii dna topoisomerase-mediated dna damage. | withangulatin a, a new compound with a known chemical structure and from the antitumor chinese herb physalis angulata l, was found to act on topoisomerase ii to induce topoisomerase ii-mediated dna damage in vitro. it has two effective dosage ranges of approximate 0.5 and 20 microm, with about one-third the activity of 20 microm vm-26. | 1989 | 2539141 |
| antigonorrhoeal activity of plants used in guatemala for the treatment of sexually transmitted diseases. | plants popularly used in guatemala for the treatment of gonorrhoea were macerated in 50% alcohol and the tincture tested for in vitro activity against neisseria gonorrhoeae using strains isolated from symptomatic patients and confirmed by standard bacteriological procedures. from 46 plants investigated, 13 (28.3%) showed evident inhibition zones (> 9 mm), seven (15.2%) showed small activity (6.1-8.9 mm) and 26 (56.5%) showed no activity; nine of these plants inhibited five strains of n. gonorrho ... | 1995 | 8583798 |
| evaluation of african medicinal plants for their in vitro trypanocidal activity. | petroleum ether, dichloromethane, methanol and water extracts from 24 plants, belonging to 19 families, which are reported in the literature as traditional remedies for sleeping sickness (human african trypanosomiasis) were screened for in vitro activity against trypanosoma brucei rhodesiense, as well as fro cytotoxicity for a human fibroblast cell-line (wi-38). the trypanocidal activity of the natural compounds berberine and harmane, both documented as being trypanocidal, was also evaluated. pr ... | 1996 | 9121161 |
| in vitro antimycobacterial activities of physalis angulata l. | the hiv-tuberculosis co-infection has caused an impact on tuberculosis epidemiology all over the world and the efficacies of the therapeutic schemes traditionally prescribed in the treatment of tuberculosis, such as isoniazid, rifampicin and pyrazinamide, have decreased due to the appearance of multidrug-resistant m. tuberculosis strains (mdr). this work is part of research on natural antimicrobial agents from plant extracts through bioassay-guided fractionation, by in vitro determination of the ... | 2000 | 10969728 |
| antimycobacterial physalins from physalis angulata l. (solanaceae). | crude extracts and fractions from aerial parts of physalis angulata have been bioassayed for antimycobacterial activity. fraction a1-29-12 containing physalins b, f and d exhibited a minimum inhibitory concentration value (mic) against mycobacterium tuberculosis h(37)rv strain of 32 microg/ml. purified physalin b and physalin d were also tested showing mic values against mycobacterium tuberculosis h(37)rv strain of > 128 microg/ml and 32 microg/ml respectively, suggesting that physalin d plays a ... | 2002 | 12203265 |
| evaluation of efficacy and safety of a herbal medicine used for the treatment of malaria. | resistance of plasmodium falciparum to chloroquine has been reported in several countries. other anti-malarial drugs in use are expensive and not readily accessible to most people in malaria endemic countries. this has led to renewed interest in the development of herbal medicines that have the potential to treat malaria with little or no side effects. this study obtained a preliminary information on the safety and effectiveness of a plant decoction (am-1), used in treating malaria. the am-1 is ... | 2003 | 12820245 |
| trypanocidal constituents in plants 4. withanolides from the aerial parts of physalis angulata. | the constituents of the aerial parts of physalis angulata (solanaceae) were investigated based on the plant's trypanocidal activity against epimastigotes of trypanosoma cruzi, the etiologic agent for chagas' disease. four new withanolides were isolated, along with six known ones, from the active fraction. their structures were determined by spectroscopic analysis. trypanocidal activity against trypomastigotes, an infectious form of t. cruzi, was also estimated, as well as cytotoxic activity agai ... | 2004 | 14758032 |
| antihepatoma activity of physalis angulata and p. peruviana extracts and their effects on apoptosis in human hep g2 cells. | physalis angulata and p. peruviana are herbs widely used in folk medicine. in this study, the aqueous and ethanol extracts prepared from the whole plant of these species were evaluated for their antihepatoma activity. using xtt assay, three human hepatoma cells, namely hep g2, hep 3b and plc/prf/5 were tested. the results showed that ethanol extract of p. peruviana (eepp) possessed the lowest ic50 value against the hep g2 cells. interestingly, all extracts showed no cytotoxic effect on normal mo ... | 2004 | 14967200 |
| biochemical crypsis in the avoidance of natural enemies by an insect herbivore. | plant-herbivore interactions provide well studied examples of coevolution, but little is known about how such interactions are influenced by the third trophic level. here we show that larvae of the specialized lepidopteran herbivore heliothis subflexa reduce their vulnerability to natural enemies through adaptation to a remarkable and previously unknown feature of their host plant, physalis angulata: the fruits of this plant lack linolenic acid (la), which is required for the development of most ... | 2004 | 15184664 |
| anticariogenic activity of some tropical medicinal plants against streptococcus mutans. | the methanol extracts of five tropical plants, baeckea frutescens, glycyrrhiza glabra, kaempferia pandurata, physalis angulata and quercus infectoria, exhibited potent antibacterial activity against the cariogenic bacterium streptococcus mutans. in particular, g. glabra, k. pandurata and p. angulata conferred fast killing bactericidal effect against s. mutans in 2 min at 50 microg/ml of extract concentration. | 2004 | 15351117 |
| mechanisms of the anti-inflammatory effects of the natural secosteroids physalins in a model of intestinal ischaemia and reperfusion injury. | reperfusion of an ischaemic tissue is associated with an intense inflammatory response and inflammation-mediated tissue injury. physalins, a group of substances with secosteroidal chemical structure, are found in physalis angulata stems and leaves. here, we assessed the effects of physalins on the local, remote and systemic injuries following intestinal ischaemia and reperfusion (i/r) in mice and compared with the effects of dexamethasone. following i/r injury, dexamethasone (10 mg kg(-1)) or ph ... | 2005 | 16025143 |
| effect of some medicinal plants on plasma antioxidant system and lipid levels in rats. | several inflammatory diseases are thought to be related to oxidative injury and free oxygen radicals have been proposed as important causative agents of heart disease and aging. to investigate the effects of daily intake of medicinal plants on antioxidant enzymes, lipid peroxidation and lipid profiles in rat, 28 rats were randomly divided into four groups and administered with three plant extracts (0.2 g/kg body weight): piper cubeba (fruit), physalis angulata (flower), rosa hybrida (flower) and ... | 2005 | 16106392 |
| antinociceptive effect of the aqueous extract obtained from roots of physalis angulata l. on mice. | in this study, we attempted to identify the possible antinociceptive action of aqueous extract (ae) obtained from roots of physalis angulata, known in brazil as "camapu", used to treat various pain-related physiological conditions. the ae of physalis angulata (10-30 mg/kg) given by i.p. or p.o. route, 0.5 and 1h prior, produced significant inhibition of abdominal constrictions caused by acetic acid, with id(50) values of 18.5 (17.4-19.8) and 21.5 (18.9-24.4)mg/kg and inhibitions of 83+/-8 and 66 ... | 2006 | 16169699 |
| trypanosoma rangeli: effects of physalin b on the immune reactions of the infected larvae of rhodnius prolixus. | physalins are seco-steroids obtained from plants of the family solanaceae. herein, we tested physalis angulata l purified physalin b as an immunomodulatory compound in 5th-instar larvae of rhodnius prolixus, which were systemically infected with the h14 trypanosoma rangeli strain protozoan. in uninfected insects, the effective concentration of physalin b, which inhibited 50% of the blood ingested (ed(50)) volume, was 15.2+/-1.6 microg/ml of the meal. ecdysis processes and mortality in uninfected ... | 2006 | 16271717 |
| studies on antimicrobial activity, in vitro, of physalis angulata l. (solanaceae) fraction and physalin b bringing out the importance of assay determination. | complex physalin metabolites present in the capsules of the fruit of physalis angulata l. have been isolated and submitted to a series of assays of antimicrobial activity against pseudomonas aeruginosa atcc 27853, staphylococcus aureus atcc 29213, s. aureus atcc 25923, s. aureus atcc 6538p, neisseria gonorrhoeae atcc 49226, escherichia coli atcc 8739; e. coli atcc 25922, candida albicans atcc 10231 applying different methodologies such as: bioautography, dilution broth, dilution agar, and agar d ... | 2006 | 16410969 |
| physalis angulata induced g2/m phase arrest in human breast cancer cells. | physalis angulata (pa) is employed in herbal medicine around the world. it is used to treat diabetes, hepatitis, asthma and malaria in taiwan. we have evaluated pa as a cancer chemopreventive agent in vitro by studying the role of pa in regulation of proliferation, cell cycle and apoptosis in human breast cancer cell lines. pa inhibited cell proliferation and induced g2/m arrest and apoptosis in human breast cancer mad-mb 231 and mcf-7 cell lines. in this study, under treatment with various conc ... | 2006 | 16427178 |
| physalins b, f and g, seco-steroids purified from physalis angulata l., inhibit lymphocyte function and allogeneic transplant rejection. | physalis angulata is a solanaceae widely used in folk medicine in various tropical countries in the world. we have previously described that seco-steroids (physalins) purified from p. angulata are potent inhibitors of macrophage activation, blocking the production of pro-inflammatory cytokines and lps-induced lethality. herein we investigated the immunomodulatory activities of these substances in lymphocyte proliferation and cytokine production and in transplantation. the addition of physalins b ... | 2006 | 16428076 |
| in-vitro and in-vivo antitumour activity of physalins b and d from physalis angulata. | we have evaluated the in-vitro and in-vivo antitumour activity of physalin b and physalin d isolated from the aerial parts of physalis angulata. in-vitro, both compounds displayed considerable cytotoxicity against several cancer cell lines, showing ic50 values in the range of 0.58 to 15.18 microg ml(-1) for physalin b, and 0.28 to 2.43 microg ml(-1) for physalin d. the antitumour activity of both compounds was confirmed in-vivo using mice bearing sarcoma 180 tumour cells. the in-vivo antitumour ... | 2006 | 16451752 |
| trypanocidal constituents in plants 6. 1) minor withanolides from the aerial parts of physalis angulata. | further study of the methanol extract of the aerial parts of physalis angulata (solanaceae) resulted in the isolation of new withanolides, designated physagulins l, m and n, together with known withanolide, physagulin d and flavonol glycoside, quercetin 3-o-rhamnosyl-(1-->6)-galactoside. the chemical structures of these new withanolides were elucidated by detailed spectroscopic analyses to be (20r,22r)-15alpha-acetoxy-5alpha,6beta,14beta,17beta,27-pentahydroxy-1-oxo-witha-2, 24-dienolide, (20s,2 ... | 2006 | 16880678 |
| high-throughput bioluminescence screening of ubiquitin-proteasome pathway inhibitors from chemical and natural sources. | to discover original inhibitors of the ubiquitin-proteasome pathway, the authors have developed a cell-based bioluminescent assay and used it to screen collections of plant extracts and chemical compounds. they first established a dld-1 human colon cancer cell line that stably expresses a 4ubiquitin-luciferase (4ub-luc) reporter protein, efficiently targeted to the ubiquitin-proteasome degradation pathway. the assay was then adapted to 96- and 384-well plate formats and calibrated with reference ... | 2007 | 17175525 |
| cytotoxic withanolides from physalis angulata l. | four new withanolides, physagulins l-o (1-4), were isolated from the meoh extract of the aerial parts of physalis angulata l. (solanaceae), together with seven known withanolides, compounds 5-11. their structures were determined by spectroscopic techniques, including 1h-, 13c-nmr (dept), and 2d-nmr (hmbc, hmqc, 1h,1h-cosy, noesy) experiments, as well as by hr-ms. all eleven compounds were tested for their antiproliferative activities towards human colorectal-carcinoma (hct-116) and human non-sma ... | 2007 | 17372946 |
| isolation, structures, and structure - cytotoxic activity relationships of withanolides and physalins from physalis angulata. | phytochemical investigation of physalis angulata was initiated following primary biological screening. fractionation of chcl3 and n-buoh solubles of the meoh extract from the whole plant was guided by in vitro cytotoxic activity assay using cultured hone-1 and nugc cells and led to the isolation of seven new withanolides, withangulatins b-h (1-7), and a new minor physalin, physalin w (8), along with 14 known compounds, including physaprun a, withaphysanolide, dihydrowithanolide e, physanolide a, ... | 2007 | 17580910 |
| trypanocidal activity of a new pterocarpan and other secondary metabolites of plants from northeastern brazil flora. | two hundred fifteen compounds isolated from plants of northeastern brazil flora have been assayed against epimastigote forms of trypanosoma cruzi, using the tetrazolium salt mtt as an alternative method. eight compounds belonging to four different species: harpalyce brasiliana (fabaceae), acnistus arborescens and physalis angulata (solanaceae), and cordia globosa (boraginaceae) showed significant activity. among them, a novel and a known pterocarpan, a chalcone, four withasteroids, and a meroter ... | 2008 | 18053728 |
| immune depression in rhodnius prolixus by seco-steroids, physalins. | a comparative study of the effects of physalins, seco-steroidal substances of physalis angulata (solanaceae), on the immune reactions of r. prolixus was carried out. ecdysis and mortality were not affected by treatment with physalins b, d, f or g (1-10 microg/ml of blood meal). r. prolixus larvae fed with blood containing physalins and inoculated with 1 microl of enterobacter cloacae beta12 (5 x 10(3)/insect) exhibited mortality rates three times higher than controls. the insects treated with ph ... | 2008 | 18234209 |
| withangulatin i, a new cytotoxic withanolide from physalis angulata. | a new withanolide, withangulatin i (2), was isolated from the whole plant of physalis angulata. its structure was established as (20s,22r)-15alpha-acetoxy-5beta,6beta-epoxy-14alpha-hydroxy-1,4-dioxo-witha-2,16,24-trienolide on the basis of chemical and spectroscopic methods including 2d-nmr and circular dichroism (cd) experiments. withangulatin a (1) and withangulatin i (2) were tested for their cytotoxic activities against two human cancer cell lines, colorectal carcinoma colo 205 and gastric c ... | 2008 | 18239318 |
| physalis angulata extract exerts anti-inflammatory effects in rats by inhibiting different pathways. | physalis angulata is a popular medicine used in brazil due to its anti-inflammatory effects, but the pharmacological mechanisms underlying these actions remain to be better understood. in the present work, lyophilized aqueous extract from the roots of physalis angulata linneu (aepa) was used to control the inflammatory response induced by the injection of 1% carrageenan into subcutaneous rat's air pouches. adenosine deaminase (ada) activity, nitrite level, and prostaglandin e(2) (pge(2)) level w ... | 2008 | 18513903 |
| genotoxic effect of physalis angulata l. (solanaceae) extract on human lymphocytes treated in vitro. | physalis angulata l (solanaceae) is a medicinal plant from north of brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. however, the genotoxic effects of p. angulata on human cells is not well known. the main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of p. angulata using the comet assay and the micronucleus assay in human lymphocyte ... | 2008 | 18825913 |
| oxidative stress involvement in physalis angulata-induced apoptosis in human oral cancer cells. | in this report, we investigated the role of oxidative stress in physalis angulata-induced apoptosis of human oral cancer cells. p. angulata-induced apoptosis was characterized by nuclear morphological changes, membrane blebbing and activation of caspase-9. exposure of hsc-3 cells to p. angulata caused production of reactive oxygen species and up-regulation of oxidative stress markers heme oxygenase-1 (ho-1), superoxide dismutase (sod), heat shock protein 70 (hsp70) and caspase-4. down-regulation ... | 2009 | 19138722 |
| physalin b inhibits rhodnius prolixus hemocyte phagocytosis and microaggregation by the activation of endogenous paf-acetyl hydrolase activities. | the effects of physalin b (a natural secosteroidal chemical from physalis angulata, solanaceae) on phagocytosis and microaggregation by hemocytes of 5th-instar larvae of rhodnius prolixus were investigated. in this insect, hemocyte phagocytosis and microaggregation are known to be induced by the platelet-activating factor (paf) or arachidonic acid (aa) and regulated by phospholipase a(2) (pla(2)) and paf-acetyl hydrolase (paf-ah) activities. phagocytic activity and formation of hemocyte microagg ... | 2009 | 19232405 |
| patents of drugs extracted from brazilian medicinal plants. | plants synthesise a vast repertoire of chemicals with various biological activities. brazilian enormous botanical diversity facilitates the development of novel ethical drugs for the treatment of diseases in humans. | 2009 | 19441926 |
| activity of physalins purified from physalis angulata in in vitro and in vivo models of cutaneous leishmaniasis. | we have previously demonstrated the immunomodulatory effects of physalins, secosteroids purified from physalis angulata. here we investigate the antileishmanial activity of physalins in vitro and in vivo in a model of cutaneous leishmaniasis. | 2009 | 19454526 |
| anti-inflammatory function of withangulatin a by targeted inhibiting cox-2 expression via mapk and nf-kappab pathways. | withangulatin a (wa), an active component isolated from physalis angulata l., has been reported to possess anti-tumor and trypanocidal activities in model systems via multiple biochemical mechanisms. the aim of this study is to investigate its anti-inflammatory potential and the possible underlying mechanisms. in the current study, wa significantly suppressed mice t lymphocytes proliferation stimulated with lps in a dose- and time-dependent manner and inhibited pro-inflammation cytokines (il-2, ... | 2010 | 19950196 |
| investigation of the immunosuppressive activity of physalin h on t lymphocytes. | physalis angulata is an annual herb widely used in folk medicine. it is mainly used for treating rheumatoid arthritis (ra). following bioactivity-guided isolation, a representative immunosuppressive compound, physalin h was been identified from this herb medicine. the purpose of this work was to assess the immunosuppressive activity of physalin h on t cells and to explore its potential mode of action. the results showed that physalin h in a dose-dependent manner significantly inhibited the proli ... | 2010 | 19951747 |
| topical anti-inflammatory potential of physalin e from physalis angulata on experimental dermatitis in mice. | the anti-inflammatory effect of physalin e, a seco-steroid isolated from physalis angulata l. was evaluated on acute and chronic models of dermatitis induced by 12-o-tetradecanoyl-phorbol-13-acetate (tpa) and oxazolone, respectively, in mouse ear. the changes in ear edema/thickness, production of pro-inflammatory cytokines (tnf-alpha and ifn-gamma), myeloperoxidase (mpo) activity, and histological and immunohistochemical findings were analysed, as indicators of dermal inflammation. similar to de ... | 2010 | 20149612 |
| in vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine. | the in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five congolese plants were evaluated. the plants were selected following an ethnobotanical survey conducted in d.r. congo and focusing on plants used traditionally to treat malaria. the in vivo antimalarial activity of aqueous and methanolic extracts active in vitro was also determined in mice infected by plasmodium berghei berghei. | 2010 | 20430094 |
| activity of physalin f in a collagen-induced arthritis model. | the effects of physalin f (1), a steroid derivative purified from physalis angulata, were investigated in models of collagen-induced arthritis in dba/1 mice and allergic airway inflammation in balb/c mice. oral treatment with 1 or dexamethasone caused a marked decrease in paw edema and joint inflammation when compared to vehicle-treated arthritic mice. in contrast, treatment with 1 had no effect in mice with allergic airway inflammation caused by ovalbumin immunization, whereas dexamethasone sig ... | 2010 | 20681573 |
| plants used by native amazonian groups from the nanay river (peru) for the treatment of malaria. | in order to evaluate the antimalarial potential of traditional remedies used in peru, indigenous and mestizo populations from the river nanay in loreto were interviewed about traditional medication for the treatment of malaria. | 2010 | 21040768 |
| inhibitory effects of physalis angulata on tumor metastasis and angiogenesis. | ethnopharmacological relavence: physalis angulata is well-known in traditional chinese medicine as a ingredient for various herbal formulation; also, it has been shown to exhibit anti-cancer and anti-inflammatory effects. in this study, the ability of p. angulata to inhibit tumor metastasis and angiogenesis was investigated. | 2011 | 21515352 |
| amelioration of systemic lupus erythematosus by withangulatin a in mrl/lpr mice. | we have previously reported the anti-inflammatory potential and the possible underlying mechanisms of withangulatin a (wa), which is an active component isolated from physalis angulata l. here, we demonstrated that wa might improve the life quality, as well as reduced the accumulation of proteinuria symptoms and levels of anti-double-stranded dna antibodies in mrl/lpr mice. moreover, wa could improve renal histopathologic characteristics of mrl/lpr mice. intriguingly, expression of b cell-activa ... | 2011 | 21520246 |
| antimicrobial activity of essential oils of physalis angulata. l. | the need for a reduction in drug resistance led to the investigation of argemone mexicana l. as an agent against bacillus subtilis, klebsiella pneumoniae, staphylococcus aureus, pseudomonas aeruginosa, candida albicans, candida stellatoidea and candida torulopsis, using well diffusion and minimum inhibitory concentrations methods. the sensitivity of bacillus subtilis, klebsiella pneumoniae, pseudomonas aeruginosa and staphylococcus aureus to the essential oils of both the aerial and root parts w ... | 2010 | 21731161 |
| antimalarial activity of physalins b, d, f, and g. | the antimalarial activities of physalins b, d, f, and g (1-4), isolated from physalis angulata, were investigated. in silico analysis using the similarity ensemble approach (sea) database predicted the antimalarial activity of each of these compounds, which were shown using an in vitro assay against plasmodium falciparum. however, treatment of p. berghei-infected mice with 3 increased parasitemia levels and mortality, whereas treatment with 2 was protective, causing a parasitemia reduction and a ... | 2011 | 21954931 |
| physalin b from physalis angulata triggers the noxa-related apoptosis pathway of human melanoma a375 cells. | melanoma is a lethal form of skin cancer that can metastasize rapidly. while surgery and radiation therapy provide palliative therapy for local tumor growth, systemic therapy is the mainstay of treatment for metastatic melanoma. however, limited chemotherapeutic agents are available for melanoma treatment. in this study, we investigated the anti-melanoma effect of physalin b, the major active compound from a widely used herb medicine, physalis angulata l. this study demonstrated that physalin b ... | 2012 | 22245079 |
| isolation and characterization of a bactericidal withanolide from physalis virginiana. | physalis virginiana (virginia groundcherry) is a member of the family solenaceae. several species of the physalis genus have been used traditionally by american indians as medicinal treatments. | 2012 | 22438659 |
| physalin f, a seco-steroid from physalis angulata l., has immunosuppressive activity in peripheral blood mononuclear cells from patients with htlv1-associated myelopathy. | human t-lymphotropic virus type 1 (htlv-1) induces a strong activation of the immune system, especially in individuals with htlv-1-associated myelopathy/tropical spastic paraparesis (ham/tsp). physalin f is a secosteroid with potent anti-inflammatory and immunomodulatory activities. the present study aimed to investigate the effects of physalin f on peripheral blood mononuclear cells (pbmc) of ham/tsp subjects. a concentration-dependent inhibition of spontaneous proliferation of pbmc from ham/ts ... | 2016 | 27044821 |
| physalis angulata induces death of promastigotes and amastigotes of leishmania (leishmania) amazonensis via the generation of reactive oxygen species. | leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus leishmania. the therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. natural products from plants have exhibited activities against leishmania in various experimental models. physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. however, its mechanis ... | 2016 | 26765293 |
| in vitro biological action of aqueous extract from roots of physalis angulata against leishmania (leishmania) amazonensis. | leishmaniasis is an infectious disease caused by various species of the protozoan parasites of the leishmania genus and transmitted by phlebotomine sandflies. the protozoa multiply in phagocytic cells, mainly macrophages, which play an important role defending the organism from pathogens. the most effective treatment for leishmaniasis is the chemotherapy and besides the high cost, these drugs are toxic and require a long period of treatment. currently, some herbal products are considered an impo ... | 2015 | 26205771 |
| genotoxicity and antileishmanial activity evaluation of physalis angulata concentrated ethanolic extract. | antileishmanial in vitro tests, as well as ames and micronucleus assays were performed with a concentrated ethanolic extract of physalis angulata (eepa) results: eepa did not present mutagenic effect in salmonella typhimurium strains at concentration reaching 3000 μg/plate and did not induce mutagenic effects after two oral administrations with a 24h interval at a dose level of 2000 mg/kg. eepa presented antileishmanial activity and presented an ic₅₀ value of 5.35 ± 2.50 μg/ml and 4.50 ± 1.17 μg ... | 2013 | 24231691 |
| in vitro and in vivo antiparasitic activity of physalis angulata l. concentrated ethanolic extract against trypanosoma cruzi. | the current treatment of chagas disease, endemic in latin america and emerging in several countries, is limited by the frequent side effects and variable efficacy of benznidazole. natural products are an important source for the search for new drugs. | 2015 | 26407938 |
| physalins b and f, seco-steroids isolated from physalis angulata l., strongly inhibit proliferation, ultrastructure and infectivity of trypanosoma cruzi. | we previously observed that physalins have immunomodulatory properties, as well as antileishmanial and antiplasmodial activities. here, we investigated the anti-trypanosoma cruzi activity of physalins b, d, f and g. we found that physalins b and f were the most potent compounds against trypomastigote and epimastigote forms of t. cruzi. electron microscopy of trypomastigotes incubated with physalin b showed disruption of kinetoplast, alterations in golgi apparatus and endoplasmic reticulum, follo ... | 2013 | 24001147 |
| physalin b inhibits trypanosoma cruzi infection in the gut of rhodnius prolixus by affecting the immune system and microbiota. | physalin b is a natural secosteroidal, extracted from the solanaceae plant, physalis angulata, and it presents immune-modulator effects on the bloodsucking bug, rhodnius prolixus. in this work, r. prolixus was treated with physalin b at a concentration of 1 mg/ml of blood meal (oral application), or 20 ng/insect (applied topically) or 57 ng/cm(2) of filter paper (contact treatment), and infected with trypanosoma cruzi dm28c clone (2×10(6) epimastigotes/insect). the three types of applications si ... | 2012 | 23085484 |
| identification and quantification of carotenoids, by hplc-pda-ms/ms, from amazonian fruits. | the major and minor carotenoids from six fruits, buriti (mauritia vinifera), mamey (mammea americana), marimari (geoffrola striata), peach palm (bactrys gasipaes), physalis (physalis angulata), and tucuma (astrocaryum aculeatum), all native to the amazonia region, were determined by high-performance liquid chromatography-photodiode array detector-mass spectrometry detector (hplc-pda-ms/ms), fulfilling the recommended criteria for identification. a total of 60 different carotenoids were separated ... | 2007 | 17530774 |
| molluscicidal activity of physalis angulata l. extracts and fractions on biomphalaria tenagophila (d'orbigny, 1835) under laboratory conditions. | the main objective of this research is to evaluate the molluscicide activity of physalis angulata l. biomphalaria tenagophila specimens under laboratory conditions. extracts and fractions were supplied by the laborat rio de qu mica de produtos naturais, farmanguinhos-fiocruz. experiments were performed according to the methodology described by the world health organization for molluscicide tests using the concentrations from 0.1 to 500 mg/l of the extracts, fractions and of a pool of physalins m ... | 2003 | 12886428 |
| antimalarial plants used by indigenous people of the upper rio negro in amazonas, brazil. | this is the first intercultural report of antimalarial plants in this region. the aim of this study was to document the medicinal plants used against malaria by indigenous people in the upper rio negro region and to review the literature on antimalarial activity and traditional use of the cited species. | 2016 | 26656535 |
| ovipositional response of threeheliothis species (lepidoptera: noctuidae) to allelochemicals from cultivated and wild host plants. | the role of plant allelochemicals on the oviposition behavior ofheliothis virescens (f.),h. subflexa (guenee), andh. zea (boddie) was investigated in the laboratory using a "choice" bioassay system. fresh young leaves of tobacco,desmodium tortuosum (swartz) de candolle, groundcherry (physalis angulata l.), and cotton (gossypium hirsutum l.) squares (flower buds) were washed in methylene chloride or methanol, concentrated to 1 g equivalent of washed material, and applied to a cloth oviposition su ... | 1990 | 24263986 |
| target discovery of cytotoxic withanolides from physalis angulata var. villosa via reactivity-based screening. | the reactivity-based screening (rbs) was developed for directed discovery of cytotoxic withanolides. in this study, a thiol probe, 4-chlorobenzenethiol, was used to selectively attack cytotoxic withanolides containing potential pharmacophore, 2(3)-en-1-one in ring a (aeo) and 5β,6β-epoxy in ring b (be), from the plant extract of physalis angulata var. villosa. the screening was performed based on the potential mechanism of 4-chlorobenzenethiol nucleophilic addition to aeo, followed by detection ... | 2018 | 29367159 |
| the genetic architecture of ecological adaptation: intraspecific variation in host plant use by the lepidopteran crop pest chloridea virescens. | intraspecific variation in ecologically important traits is a cornerstone of darwin's theory of evolution by natural selection. the evolution and maintenance of this variation depends on genetic architecture, which in turn determines responses to natural selection. some models suggest that traits with complex architectures are less likely to respond to selection than those with simple architectures, yet rapid divergence has been observed in such traits. the simultaneous evolutionary lability and ... | 2017 | 29238078 |
| germination biology of two invasive physalis species and implications for their management in arid and semi-arid regions. | two solanaceae invasive plant species (physalis angulata l. and p. philadelphica lam. var. immaculata waterfall) infest several arable crops and natural habitats in southeastern anatolia region, turkey. however, almost no information is available regarding germination biology of both species. we performed several experiments to infer the effects of environmental factors on seed germination and seedling emergence of different populations of both species collected from various locations with diffe ... | 2017 | 29208989 |
| unprecedented 22,26-seco physalins from physalis angulata and their anti-inflammatory potential. | two novel physalins, including a 22,26-seco physalin, physalin x (1), and a 11,15-cyclo-9(10),14(17),22(26)-triseco physalin with an unprecedented aromatic ring, aromaphysalin b (2), were isolated from physalis angulata l. their structures were determined by ir, uv, hresims, and 2d nmr spectra as well as theoretical calculations. compounds 1 and 2 exhibited inhibitory activities on no production with ic50 values of 68.50 and 29.69 μm, respectively. a plausible biosynthetic pathway for 2 is also ... | 2017 | 28991309 |
| downregulation of tigar sensitizes the antitumor effect of physapubenolide through increasing intracellular ros levels to trigger apoptosis and autophagosome formation in human breast carcinoma cells. | physapubenolide (pb) is a cytotoxic withanolide isolated from physalis angulata that was used as a traditional chinese medicine. in this study, we investigated the role of tigar and ros in pb-induced apoptosis and autophagosome formation in human breast carcinoma mda-mb-231 and mcf-7 cells. pb induced apoptosis by decreasing mitochondrial membrane potential and elevating the bax/bcl-2 protein expression ratio in mda-mb-231 and mcf-7 cells. caspase inhibitor z-vad-fmk treatment partly blocked pb ... | 2017 | 28774732 |
| intestinal anti-inflammatory activity of ground cherry (physalis angulata l.) standardized co2 phytopharmaceutical preparation. | to investigate the effects of ground cherry (physalis angulata l.) standardized supercritical co2 extract in trinitrobenzenesulphonic acid (tnbs) model of rat intestinal inflammation. | 2017 | 28706419 |
| physalins v-ix, 16,24-cyclo-13,14-seco withanolides from physalis angulata and their antiproliferative and anti-inflammatory activities. | five new physalins, including a novel 1,10-seco one, physalin v (1), a tricarboxylic acid cycle one, physalin viii (5), a rare 11,15-cyclo one, physalin ix (6), and two new ones, physalins vi (2) and vii (4) were isolated from stems and leaves of physalis angulata together with eleven known analogues (3 and 7-16). their structures were established by ms, ir, uv, and nmr spectroscopic analysis, together with the x-ray diffraction analysis of neophysalin, physalin p (12), and the structure of phys ... | 2017 | 28642618 |
| cytotoxic withanolides from physalis angulata. | a new withanolide (1), physagulide p, together with five known withanolides (2-6), was isolated from the aerial parts of physalis angulata l. the structure of new compound was elucidated on the basis of extensive spectroscopic techniques, including 1d, 2d nmr and hresims. the activity screening indicated that compound 1 showed significant cytotoxicities against the human osteosarcoma cell line mg-63, hepg-2 hepatoma cells and breast cancer cells mda-mb-231 with the ic50 value of 3.50, 4.22 and 1 ... | 2018 | 28617049 |
| anti-inflammatory effects of physalin e from physalis angulata on lipopolysaccharide-stimulated raw 264.7 cells through inhibition of nf-κb pathway. | physalin e is a naturally occurring seco-steroid isolated from the stems and aerial parts of physalis angulata l. (solanaceae). this study was aimed to explore the anti-inflammatory effects of physalin e on raw 264.7 mouse macrophages stimulated by lipopolysaccharide (lps) and the potential underlying mechanisms. the results showed that physalin e significantly inhibited lps-induced tumor necrosis factor-α (tnf-α) and interleukin-6 (il-6) expression and secretion in a dose-dependent manner. unli ... | 2017 | 28152630 |
| a novel withanolide with an unprecedented carbon skeleton from physalis angulata. | a novel withanolide, aromaphysalin a (1), possessing an exceptional c(11)-c(15) bond and an unprecedented 4,9-cyclized aromatic ring (ring a), is isolated from stems and leaves of physalis angulata l. its structure was determined by a combination of hresims, 2d nmr spectra, and theoretical calculations. compound 1 exhibited inhibitory activity on no production with an ic50 value of 51.64 μm. a plausible biosynthetic pathway for 1 is also discussed. | 2017 | 28098317 |
| a new phenol glycoside from physalis angulata. | a new phenol glycoside, physanguloside a (1), was isolated from physalis angulata together with four known compounds. we report herein, for the first time, the presence of compounds 2-5 in the genus physalis. the structures of all the compounds were established by nmr, ir, uv and hresims spectroscopic analyses, and comparison with the literature data. all isolated compounds were assayed for inhibitory activity on nitric oxide production by lps-induced in raw 264.7 macrophages. | 2017 | 28033720 |
| antiproliferative and anti-inflammatory withanolides from physalis angulata. | sixteen new withanolides, physangulatins a-n (1-14) and withaphysalins y and z (15 and 16), as well as 12 known analogues, were isolated from the stems and leaves of physalis angulata l. their structures were established using extensive spectroscopic data analyses. the absolute configurations of 1 and 9 were assigned via x-ray crystallography. the isolated compounds were tested for their antiproliferative effects against human prostate cancer cells (c4-2b and 22rvl), human renal carcinoma cells ... | 2016 | 27295506 |
| effect of osmopriming on germination and initial growth of physalis angulata l. under salt stress and on expression of associated genes. | this study aimed to evaluate the effects of priming on seed germination under salt stress and gene expression in seeds and seedlings of p. angulata l. after priming for 10 days, seed germination was tested in plastic trays containing 15 ml of water (0 ds m-1 - control) or 15 ml of nacl solution (2, 4, 6, 8, 10, 12, 14 and 16 ds m-1). fresh and dry weight of shoots and roots of seedlings were evaluated at 0, 2, 4, 6, 8 ds m-1. total rna was extracted from whole seeds and seedlings followed by rt- ... | 2016 | 27142542 |
| anti-oxidative and reno-restorative effects of physalis angulata (whole plant extract) in alloxan-induced diabetic male wistar rats. | backgroiound: hyperglycemia has been reported to increase protein glycation and generation of free radicals which predispose to diabetic renal dysfunction. physalis ahgulata has been shown to have hypoglycacmic and anti-lipidemic properties but there is dearth of information regarding its effect on kidney functions in diabetes. this study investigated the anti-oxidative and reno-restorative effects of methanol extract of whole plant of physalis angulata (mepa) in alloxan-induced diabetic rats. | 2016 | 28686833 |
| screening of korean natural products for anti-adipogenesis properties and isolation of kaempferol-3-o-rutinoside as a potent anti-adipogenetic compound from solidago virgaurea. | in this study, the anti-adipogenetic activity of 300 plant extracts was investigated using an oil red o staining assay in a 3t3-l1 cell line. our results indicate that three plants, including the stem and leaf of physalis angulata, the whole grass of solidago virgaurea, and the root of dioscorea nipponica, produced over 90% inhibition of adipogenesis. kaempferol-3-o-rutinoside, which demonstrated a 48.2% inhibitory effect on adipogenesis without cytotoxicity, was isolated from the butanol layer ... | 2016 | 26901177 |
| three new cytotoxic withanolides from the chinese folk medicine physalis angulata. | physagulides m-o, three new withanolides (1-3), were isolated from the aerial parts of physalis angulata l. their structures were elucidated through extensive spectroscopic techniques, including 1d and 2d nmr, and hresims. the absolute configurations (22-r) of these new compounds were determined by cd analysis. compounds 1 and 3 showed significant selective cytotoxic activities on the mg-63 cell line, with ic50 values of 4.28 and 5.44 μm, respectively. | 2015 | 26882664 |
| cytotoxic 20,24-epoxywithanolides from physalis angulata. | a new withanolide, physangulide b (1), was isolated from calyxes of physalis angulata. this compound was also present in the aerial parts along with the known physangulide (4), which was isolated as the acetonide 3, and 24,25-epoxywithanolide d (6). structures of these compounds were determined by analysis of their spectroscopic data, which indicated the presence of a 20,24-epoxy group in both physangulides. the structures of compounds 1 and 6 were confirmed by x-ray analysis of their correspond ... | 2015 | 26335153 |
| antinociceptive properties of physalins from physalis angulata. | pain is the most common reason a patient sees a physician. nevertheless, the use of typical painkillers is not completely effective in controlling all pain syndromes; therefore further attempts have been made to develop improved analgesic drugs. the present study was undertaken to evaluate the antinociceptive properties of physalins b (1), d (2), f (3), and g (4) isolated from physalis angulata in inflammatory and centrally mediated pain tests in mice. systemic pretreatment with 1-4 produced dos ... | 2014 | 25396337 |
| physalis angulata induces in vitro differentiation of murine bone marrow cells into macrophages. | the bone marrow is a hematopoietic tissue that, in the presence of cytokines and growth factors, generates all of the circulating blood cells. these cells are important for protecting the organism against pathogens and for establishing an effective immune response. previous studies have shown immunomodulatory effects of different products isolated from plant extracts. this study aimed to evaluate the immunomodulatory properties of aqueous physalis angulata (aepa) extract on the differentiation o ... | 2014 | 25281406 |
| unprecedent aminophysalin from physalis angulata. | the 95% ethanol extract of the whole plant of physalis angulata linn. afforded one new skeletal physalin named aminophysalin a (1) and one new naturally occurring 5β-hydroxy-6a-chloro-5,6-dihydrophysalin b (2), together with five known physalins (3-7). their structures were elucidated through ms, ir, nmr spectroscopy analyses and x-ray crystallography. aminophysalin a (1) had an absolutely unusual structural feature in the chemistry of physalins with a nitrogen atom. compounds 1-7 were evaluated ... | 2014 | 24973634 |
| induction of quinone reductase (qr) by withanolides isolated from physalis angulata l. var. villosa bonati (solanaceae). | in the present study, the etoac extract of the persistent calyx of physalis angulata l. var. villosa bonati (pa) was tested for its potential quinone reductase (qr) inducing activity with glutathione (gsh) as the substrate using an uplc-esi-ms method. the result revealed that the pa had electrophiles that could induce quinone reductase (qr) activity, which might be attributed to the modification of the highly reactive cysteine residues in keap1. herein, three new withanolides, compounds 3, 6 and ... | 2014 | 24793335 |
| isolation, pharmacological activity and structure determination of physalin b and 5β,6β-epoxyphysalin b isolated from congolese physalis angulata l. | physalis angulata l., an annual herb from the solanaceae family, is widely used in popular medicine in tropical countries to treat a variety of diseases. two products, (x) and (y), were isolated from a crude ch2cl2 extract of dried congolese physalis angulata l. plants and crystallized from acetone for structure elucidation. compound (x) corresponds to a physalin b dimer acetone solvate hydrate (2c28h30o9·c3h6o·0.22h2o), while compound (y) crystallizes as a mixed crystal containing two physalin ... | 2013 | 24311512 |
| physangulidine a, a withanolide from physalis angulata, perturbs the cell cycle and induces cell death by apoptosis in prostate cancer cells. | recently, our group reported the discovery of three new withanolides, physangulidines a-c, from physalis angulata. in this study, the biological effects of physangulidine a (1), which was the most active and abundant of the three new constituents, are described. it was found that 1 significantly reduces survival in clonogenic assays for two hormone-independent prostate cancer cell lines. flow cytometry and confocal microscopy studies in du145 human prostate cancer cells indicated that 1 induces ... | 2013 | 23270478 |
| physalin f induces cell apoptosis in human renal carcinoma cells by targeting nf-kappab and generating reactive oxygen species. | the aim of this study was to determine the molecular mechanisms of physalin f, an effective purified extract of physalis angulata l. (solanacae), in renal carcinoma a498 cells. | 2012 | 22815798 |
| physangulidines a, b, and c: three new antiproliferative withanolides from physalis angulata l. | bioassay-directed fractionation of the whole plant of physalis angulata l. afforded three new antiproliferative withanolides with an unusual carbon framework: physangulidines a (1), b (2), and c (3). structures of the three isomeric withanolides were determined by a combination of hrms, nmr spectroscopic, and x-ray crystallographic methods. each has shown significant antiproliferative activity against du145 prostate cancer cells. physangulidine a (1) was further tested against a wide range of ad ... | 2012 | 22329497 |
| physanolide a, a novel skeleton steroid, and other cytotoxic principles from physalis angulata. | [reaction: see text] a novel withasteroid, physanolide a (1), with an unprecedented skeleton containing a seven-membered ring, and two new physalins, physalins u (2) and v (3), were isolated from physalis angulata. the structures were elucidated from spectroscopic analysis, and plausible biosynthetic pathways were postulated. physalins b (4), d (5), and f (6) showed strong cytotoxicity against multiple tumor cell lines, including kb, a431, hct-8, pc-3, and zr751, with ec(50) values less than 4 m ... | 2006 | 16805525 |
| investigations of anti-inflammatory and antinociceptive activities of piper cubeba, physalis angulata and rosa hybrida. | the anti-inflammatory activities of piper cubeba (fruit), physalis angulata (flower) and rosa hybrida (flower) were determined by carrageenan-induced paw edema, arachidonic acid-induced ear edema and formaldehyde-induced arthritis in mice. the anti-allergic and analgesic activities of these plants were also studied by using 2,4-dinitrofluorobenzene (dnfb)-induced contact hypersensitivity reaction (type iv) and hot plate test in mice, respectively. these plant extracts clearly exhibited inhibitor ... | 2003 | 14522451 |
| inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from physalis angulata l. | physalis angulata l. is an annual herb widely used in popular medicine for the treatment of a variety of pathologies. here, we tested immunomodulatory activities of physalins, seco-steroids purified from p. angulata extracts. addition of physalins b, f or g, but not d, caused a reduction in nitric oxide production by macrophages stimulated with lipopolysaccaride and interferon-gamma. in the presence of physalin b, macrophages stimulated with lipopolysaccaride, alone or in combination with interf ... | 2003 | 12505539 |
| a novel cytotoxic flavonoid glycoside from physalis angulata. | a new flavonol glycoside, myricetin 3-o-neohesperidoside (1) was isolated from a cytotoxic meoh extract of the leaves of physalis angulata. compound 1 showed remarkable cytotoxicity in vitro against murine leukemia cell line p-388, epidermoid carcinoma of the nasopharynx kb-16 cells, and lung adenocarcinoma a-549 with ed(50) values of 0.048, 0.50 and 0.55 microg ml(-1), respectively. | 2001 | 11543968 |
| immunomodulatory activity of various fractions derived from physalis angulata l extract. | the immunomodulatory effects of physalis angulata l. extract fraction vii (pa-vii), pa-vii-a, pa-vii-b and pa-vii-c were investigated in this study. the results showed that pa-vii and pa-vii-c strongly enhanced blastogenesis response, pa-vii-b had moderate activity, and pa-vii-a exerted only slight effect on cell proliferation. a synergistic effect was observed when the suboptimal dosage of phytohemagglutinin (pha) or lipopolysaccharide (lps) was added to the culture. furthermore, pa-vii and pa- ... | 1992 | 1471607 |
| flight response ofheliothis subflexa (gn.) females (lepidoptera: noctuidae) to an attractant from groundcherry,physalis angulata l. | mated femaleheliothis subflexa (gn.) (hs) moths 1-7 days old responded positively in a plexiglas flight tunnel to an attractant extracted with methanol from fresh whole-leaf washes of groundcherry,physalis angulata l. response to the groundcherry extract, as indicated by plume-tracking (i.e., upwind flight toward the odor source) and contact with the chemical dispenser did not change significantly during the first 5 hr of scotophase. overall, ca. 50% of the responding moths also landed on the ch ... | 1989 | 24271437 |
| heliothis subflexa (gn.) (lepidoptera: noctuidae): demonstration of oviposition stimulant from groundcherry using novel bioassay. | methanol extracts of homogenized groundcherry,physalis angulata, leaves increased egg deposition byheliothis subflexa (gn.) (hs) on treated tobacco plants (a nonhost) 8.5-fold over untreated controls. in doseresponse tests using whole-leaf washes of groundcherry leaves, the threshold of positive response vs. no response to the chemical stimulant was within one log dose unit when compared to the controls. this response was consistent whether the chemical was evaluated on plants in greenhouse-cage ... | 1987 | 24302394 |