| [diversity of siderophore-producing endophytic bacteria of cymbidium goeringii roots]. | in order to obtain the effective plant growth-promoting bacteria, we studied the diversity of siderophore-producing endophytic bacteria in the roots of cymbidium goeringii. | 2011 | 21574379 |
| development of polymorphic microsatellite markers for cymbidium goeringii (orchidaceae). | microsatellite markers were developed in cymbidium goeringii to investigate its genetic diversity and population genetic structure. | 2012 | 22539509 |
| functional analysis of flowering locus t orthologs from spring orchid (cymbidium goeringii rchb. f.) that regulates the vegetative to reproductive transition. | the flowering locus t (ft) gene plays crucial roles in regulating the vegetative-to-reproductive phase transition. the ft-like gene of spring orchid (cymbidium goeringii rchb. f.), cgft, was isolated and characterized. cgft mrna was detected in leaves, pseudobulb, and flowers. in flowers, cgft was expressed more in young flower buds than in mature flowers, and was predominantly expressed in young ovary and sheath. seasonal expression analysis in leaves of a three-year-old spring orchid showed th ... | 2012 | 22796899 |
| chitinophaga cymbidii sp. nov., isolated from cymbidium goeringii roots. | two gram-staining-negative, aerobic, non-spore-forming rod-shaped, non-motile bacteria, designated strains r156-2(t) and t58-2 were isolated from the roots of cymbidium goeringii. the colonies were yellow-pigmented. on the basis of 16s rrna gene sequence similarity, strains r156-2(t) and t58-2 were shown to be members of the genus chitinophaga. strains r156-2(t) and t58-2 showed the greatest level of sequence similarity with chitinophaga niabensis (96.0-96.3 %). the major menaquinone was mk-7. t ... | 2013 | 22962335 |
| mycorrhizas alter nitrogen acquisition by the terrestrial orchid cymbidium goeringii. | orchid mycorrhizas exhibit a unique type of mycorrhizal symbiosis that occurs between fungi and plants of the family orchidaceae. in general, the roots of orchids are typically coarse compared with those of other plant species, leading to a considerably low surface area to volume ratio. as a result, orchids are often ill-adapted for direct nutrient acquisition from the soil and so mycorrhizal associations are important. however, the role of the fungal partners in the acquisition of inorganic and ... | 2013 | 23532045 |
| molecular characterization and functional analysis of a flowering locus t homolog gene from a phalaenopsis orchid. | warm day and cool night conditions significantly induce reproductive spike formation in phalaenopsis plants; hence, determining the flowering mechanism regulating the reproductive transition is important. flowering locus t (ft) plays important roles in flowering induction in several plants. to explore spike induction by warm days and cool nights in phalaenopsis orchids, we isolated the ft (phft) from phalaenopsis hybrid fortune saltzman. the cdna of phft was 809-bp long and contained a 531-bp op ... | 2014 | 25117357 |
| cymbidium chlorotic mosaic virus, a new sobemovirus isolated from a spring orchid (cymbidium goeringii) in japan. | cymbidium chlorotic mosaic virus (cycmv), isolated from a spring orchid (cymbidium goeringii), was characterized molecularly. cycmv isometric virions comprise a single, positive-strand rna genome of 4,083 nucleotides and 30-kda coat protein. the virus genome contains five overlapping open reading frames with a genomic organization similar to that of sobemoviruses. blast searches and phylogenetic analysis revealed that cycmv is most closely related to papaya lethal yellowing virus, a proposed dic ... | 2015 | 26025156 |
| molecular cloning and functional analysis of three flowering locus t (ft) homologous genes from chinese cymbidium. | the flowering locus t (ft) gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. to understand the molecular mechanism of reproduction, three homologous ft genes were isolated and characterized from cymbidium sinense "qi jian bai mo", cymbidium goeringii and cymbidium ensifolium "jin si ma wei". the three genes contained 618-bp nucleotides with a 531-bp open reading frame (orf) of encoding 176 amino acids (aas). alignment of the aa sequences revealed th ... | 2012 | 23109860 |
| isolation and characterization of a flowering locus t homolog from pineapple (ananas comosus (l.) merr). | flowering locus t (ft)-like genes are crucial regulators of flowering in angiosperms. a homolog of ft, designated as acft (genbank id: hq343233), was isolated from pineapple cultivar comte de paris by reverse transcriptase polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the cdna sequence of acft is 915 bp in length and contains an orf of 534 bp, which encodes a protein of 177 aa. molecular weight was 19.9 kda and isoelectric point was 6.96. the deduced protein seq ... | 2012 | 22710136 |
| Significant demographic and fine-scale genetic structure in expanding and senescing populations of the terrestrial orchid Cymbidium goeringii (Orchidaceae). | • Premise of the study: Fine-scale genetic structure (FSGS) in plants is influenced by variation in spatial and temporal demographic processes. To determine how demographic structure and FSGS change with stages of population succession, we studied replicate expanding and senescing populations of the Asian terrestrial orchid Cymbidium goeringii. • Methods: We used spatial autocorrelation methods (O-ring and kinship statistics) to quantify spatial demographic structure and FSGS in two expanding an ... | 2011 | 22106436 |
| isolation and characterization of 15 microsatellite markers from the spring orchid (cymbidium goeringii) (orchidaceae). | • premise of the study: microsatellite primers were developed for the important and popular ornamental spring orchid, cymbidium goeringii, to investigate its genetic diversity and population genetic structure. • methods and results: using the fast isolation by aflp of sequences containing (fiasco) repeats protocol, 15 primer sets were identified in two wild populations. the number of alleles per locus ranged from 2 to 5, with a mean of 3.3. the observed and expected heterozygosities varied from ... | 2011 | 21613151 |
| molecular diversity and relationships among cymbidium goeringii cultivars based on inter-simple sequence repeat (issr) markers. | spring orchid (cymbidium goeringii) is a popular flowering plant species. there have been few molecular studies of the genetic diversity and conservation genetics on this species. an assessment of the level of genetic diversity in cultivated spring orchid would facilitate development of the future germplasm conservation for cultivar improvement. in the present study, dna markers of intersimple sequence repeats (issr) were identified and the issr fingerprinting technique was used to evaluate gene ... | 2009 | 19085060 |
| structure of cymbidine a, a monomeric peptidoglycan-related compound with hypotensive and diuretic activities, isolated from a higher plant, cymbidium goeringii (orchidaceae). | the structure of a new monomeric peptidoglycan-related compound with hypotensive and diuretic activities, cymbidine a (1) isolated from the orchid cymbidium goeringii, was elucidated mainly by spectroscopic analysis. the structure of 1 was shown to involve four amino acids (d-alanin, meso-diaminopimelic acid, d-gultamic acid, and l-valine) and two amino sugars (n-acetylglucosamine and 1,6-anhydro-n-acetylmuramic acid). the sequence of the amino acids and amino sugars was determined by the analys ... | 2007 | 17473468 |
| gigantol isolated from the whole plants of cymbidium goeringii inhibits the lps-induced inos and cox-2 expression via nf-kappab inactivation in raw 264.7 macrophages cells. | during our efforts to find bioactive natural products with anti-inflammatory activity, we isolated gigantol from the whole plants of cymbidium goeringii (orchidaceae) by activity-guided chromatographic fractionation. gigantol was found to have potent inhibitory effects on lps-induced nitric oxide (no) and prostaglandin e (2) (pge (2)) production in raw 264.7 cells. consistent with these findings, gigantol suppressed the expression of inducible nitric oxide synthase (inos) and cyclooxygenase-2 (c ... | 2006 | 16924582 |
| [in vitro flowering of cultures from a hybrid of cymbidium goeringii and c. hybridium]. | wild-type female spring orchid (cymbidium goeringii) was crossed with male cymbidium hybridium. over eight hundred protocorm clones were obtained from hybrid offsprings. among them, one protocorm clone was identified to differentiate visible floral buds two months after subculture in vitro (plate i). the protocorms and shoots derived from this clone were further used in studying the effects of abscisic acid (aba) and paclobutrazol (pp333) pretreatment as well as different concentrations of 6-ben ... | 2006 | 16775400 |