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[experimental infection of biomphalaria tenagophila from argentina with strains of schistosoma mansoni from brazil]. 1979262315
[the susceptibility of the biomphalaria tenagophila (d'orbigny 1835) from itajubá, minas gerais, to infection by the le strain of schistosoma mansoni sambon 1907 from belo horizonte, brazil]. 1979462114
[susceptibility of biomphalaria tenagophila and biomphalaria glabrata to 2 strains of schistosoma mansoni]. 1979482771
[susceptibility to schistosoma mansoni from hybrids of biomphalaria tenagophila of taim, rs, cabo frio, rj and belo horizonte, mg]. 1979550285
[selection in strains of biomphalaria tenagophila and biomphalaria glabrata for improving susceptibility to schistosoma mansoni]. 1978675140
differential susceptibility of biomphalaria tenagophila populations to infection with a strain of schistosoma mansoni.experiments to determine the susceptibility of biomphalaria tenagophila from 20 localities over the species range exposed to a strain of schistosoma mansoni showed widely varying infection rates, from 0% to 91.5%, this latter having been observed in a sample from an area where the natural infection rate of b. tenagophila has been the highest in the country. comparison with similar studies involving b. glabrata seems to indicate that a process of adaptation between s. mansoni and b. tenagophila i ...1978722455
snail control in urban sites in brazil with slow-release hexabutyldistannoxane and pentachlorophenol.slow release formulations of hexabutyldistannoxane (tbto) and pentachlorophenol (pcp) were tested for the control of biomphalaria tenagophila in 52 urban sites in rio de janeiro. tbto acted faster and lasted longer than pcp and at 15 g/m(2) it eliminated snails from 76% of the treated sites for 1 year. water pollution and rate of flow had no significant influence on the molluscicidal properties of either compound, but alkalinity lowered the activity of tbto. failure to control snail populations ...19761088356
[parasitological survey, with special reference to schistosomiasis mansoni, conducted in the region of jacupiranga (ribeira valley, state of são paulo). survey of the location and distribution of planorbidian snails in the state of são paulo. vii].the results of a parasitological survey, emphasizing particularly schistosomiasis mansoni, are presented. the area under study was the district of cajati, situated in the "município" of jacupiranga (state of são paulo, brazil) near schistosomiasis-endemic areas in vale do ribeira. though the presence of parasitized people by schistosoma mansoni has been reported and focuses of biomphalaria tenagophila, the species responsible for the spreading of the parasites through great areas of the state, h ...19751241922
evaluation of temporal, seasonal and geographic stability of the molluscicidal property of euphorbia splendens latex.laboratory tests with aqueous solutions of euphorbia splendens var. hislopii latex have demonstrated seasonal stability of the molluscacidal principle, with ld90 values of 1.14 ppm (spring), 1.02 ppm (fall), 1.09 ppm (winter), and 1.07 ppm (summer) that have been determined against biomphalaria tenagophila in the field. assays on latex collected in belo horizonte and recife yielded ld90 values similar to those obtained with the reference substance collected in rio de janeiro (ilha do governador) ...19921342068
biological and morphological characteristics of schistosoma mansoni from ribeira valley, state of são paulo, brazil i--susceptibility of biomphalaria tenagophila snail to sympatric s. mansoni strain.in the são paulo state, brazil, where the biomphalaria tenagophila is the intermediate host, the ribeira valley is an important endemic schistosomiasis mansoni area. during last eleven years there has been intense control measures focusing on schistosomiasis. the efforts have been concentrated in the municipalities of pedro de toledo and itariri. we determined the susceptibility of b. tenagophila to sympatric strain of s. mansoni, both recently isolated from itariri field. in 1988, this strain w ...19921342070
natural and experimental infection of planorbids from the island of santa catarina (brazil).studies of eight localities on the island of santa catarina revealed the presence of three species of the molluscan family planorbidae: biomphalaria tenagophila, drepanotrema cimex and biomphalaria oligoza, the first one being naturally infected by cercaria ocellifera, a furcocercaria with morphological characteristics of cercaria caratinguensis, and by an unknown furcocercaria. drepanotrema cimex was infected by a furcocercaria with characteristics of c. caratinguensis and by c. macrogranulosa. ...19921342085
[control of schistosomiasis mansoni in an area of low transmission].the schistosomiasis is transmitted by biomphalaria tenagophila in our study area (pedro de toledo, são paulo, brazil). from 1980 to 1990 epidemiological surveys in a population of 4,000 inhabitants, has shown that: prevalences by kato-katz (kkt), immunofluorescence (ft) and intradermal (idt) techniques were 22.8%, 55.5% and 51.8%, respectively; intensity of infection was low, 58.5 eggs per gram of faeces (epg); there were no symptomatic cases; prevalences were higher in mates, children and rural ...19921343901
[miracidial attraction by biomphalaria tenagophila genetically selected for susceptibility].the attraction of schistosoma mansoni miracidea by snails genetically selected for susceptibility to infection is considered. miracidea were exposed to snails or to snail conditioned water in a specially devised three-chamber glass apparatus. ten miracidea were used for each test and were deposited in the middle chamber (a passage); after 15 minutes, them in each of the number of the three chambers were recorded. in one of the chambers, chosen randomly at each trial, either snails or scw were us ...19911784970
[relationship between the pathogenicity of schistosoma mansoni in mice and the susceptibility of the vector mollusc. i. cercariae infectivity and worm burden].the possible influence of the immune mechanisms of the molluscs on the development of the sporocysts and the resultant behavior of the adult worm in the vertebrate host were studied. two strains of schistosoma mansoni (bh and sj) were used. these were genetically selected for their susceptibility and maintained, respectively, in biomphalaria glabrata and biomphalaria tenagophila, the natural invertebrate hosts. experimental groups of mice infected with cercariae proceeding from the two species o ...19911820625
schistosomiasis mansoni in an area of low transmission. i. impact of control measures.this work was undertaken in the municipality of pedro de toledo (são paulo state, brazil) in 1987, to clarify aspects related to the transmission levels of schistosoma mansoni in a human population where the snail host is biomphalaria tenagophila. since 1980 a control programme has been undertaken in this municipality. urban and rural populations (4,719 subjects) were submitted to faecal examinations (kato-katz method). the overall prevalence rate was 4.8% being higher in males (6.2%) and also i ...19911844388
biological control of biomphalaria tenagophila (mollusca, planorbidae), a schistosomiasis vector, using the fish geophagus brasiliensis (pisces, cichlidae) in the laboratory or in a seminatural environment.in order to investigate a possible method of biological control of schistosomiasis, we used the fish geophagus brasiliensis (quoy & gaimard, 1824) which is widely distributed throughout brazil, to interrupt the life cycle of the snail biomphalaria tenagophila (orbigny, 1835), an intermediate host of schistosoma mansoni. in the laboratory, predation eliminated 97.6% of the smaller snails (3-8 mm shell diameter) and 9.2% of the larger ones (12-14 mm shell diameter). very promising results were als ...19902215231
schistosomiasis mansoni in the municipality of pedro de toledo (são paulo, brazil) where the biomphalaria tenagophila is the snail host. i--prevalence in human population.due to the scarce information about the epidemiological features of schistosomiasis in which the vector is biomphalaria tenagophila, an investigation was carried in pedro de toledo in 1980 where such peculiarity is observed. stool examinations (kato-katz method) were performed in 4,741 individuals (22.8% positive to schistosoma mansoni eggs) of this 583 had previously received chemotherapy and 4,158 remainders, untreated. the schistosomiasis prevalence in those two groups where respectively 31.7 ...19892513633
[distribution of biomphalaria tenagophila and b. occidentalis in the state of são paulo (brazil)].the geographical distribution of biomphalaria tenagophila (d'orbigny, 1835) and b. occidentalis paraense, 1981 in s. paulo state, brazil, is described. the first species being the most important intermediate host of schistosoma mansoni sambon, 1907. the second species is unsusceptible to transmission. the two species inhabit the limnic environments of great areas of s. paulo, occasionally in simpatria. the 3,160 samples of mollusks collected between september 1981 and march 1986 were examined. b ...19892515586
a potential vector of schistosoma mansoni in uruguay.susceptibility experiments were carried out with a biomphalaria straminea-like planorbid snail (biomphalaria aff. straminea, species inquirenda) from espinillar, near salto (uruguay), in the area of the salto grande reservoir, exposed individually to 5 miracidia of schistosoma mansoni (sj2 and bh2 strains). of 130 snails exposed to the sj2 strain, originally infective to biomphalaria tenagophila, 30 became infected (23%). the prepatent (precercarial) period ranged from 35 to 65 days. the cercari ...19892520826
study of a population of biomphalaria tenagophila (orbigny, 1835) and of schistosomiasis transmission in "alto da boa vista", rio de janeiro.the present study was performed using data from a biomphalaria tenagophila population located in a watercress garden in the alto da boa vista region representing an isolated focal point of schistosomiasis in the city of rio de janeiro. the density and age structure of this b. tenagophila population and its rate of infection by schistosoma mansoni were studied for a period of 15 months. the snail population showed seasonal variation in density, with a decrease in number of individuals at the begi ...19892520827
[cercarian chaetotaxy of schistosoma mansoni from naturally infected biomphalaria tenagophila]. 19863103354
the epidemiology and control of schistosomiasis mansoni where biomphalaria tenagophila is the snail host. 19883150615
[verification of antagonism between larvae of schistosoma mansoni and other digenea in biomphalaria tenagophila, a planorbid mollusc from a natural breeding in the region of campinas, sp, brazil]. 19883150870
[potential of biomphalaria tenagophila from pampulha lake, belo horizonte, mg, as a host of schistosoma mansoni].biomphalaria tenagophila snails, from a population originally obtained from "pampulha" lake, belo horizonte, minas gerais state, brazil, were exposed to miracidia from four strains of schistosoma mansoni: "le" and "hk" from belo horizonte, "al" from alagoas and "sj" from são josé dos campos, são paulo. the "le", "al" and "sj" strains are maintained in the laboratory and the "hk" strain was obtained from feces of a patient residing near to "pampulha" lake. infection rates were of 4% ("le" strain) ...19873507567
population dynamics of aquatic snails in pampulha reservoir.an attempt was made to determine more accurately the density of molluskan populations in the pampulha reservoir, using the quadrate method, intending to detect the fluctuation of the populations density, the habitat conditions and the possible competitive interactions among biomphalaria tenagophila, melanoides tuberculata, pomacea haustrum and biomphalaria glabrata, through the analysis of populational parameters. among the most significative facts observed in the reservoir it has to be mentione ...19873509186
[1st finding of biomphalaria tenagophila (d'orbigny, 1835) naturally infected with schistosoma mansoni, in itajubá, in the south of the state of minas gerais, brazil]. 19853936162
on the finding of biomphalaria tenagophila naturally infected with schistosoma mansoni in the state of minas gerais, brazil. 19853937209
[susceptibility of biomphalaria tenagophila hybrids to the le (bh) strain of schistosoma mansoni]. 19854035208
[biomphalaria tenagophila naturally infected with schistosomias mansoni in cubatão, state of são paulo, brazil]. 19675629112
[susceptibility of biomphalaria tenagophila from the state of são paulo to infection by schistosoma mansoni strains from the baixada maranhense (maranhão, brazil]. 19846531638
[susceptibility of biomphalaria tenagophila from belo horizonte and vicinities to infection with 3 strains of schistosoma mansoni]. 19836658299
[evolution of spopocysts of schistosoma mansoni sambon 1907 in biomphalaria glabrata (say 1818) and biomphalaria tenagophila (d'orbigny 1835)]. 19817336119
[susceptibility to schistosoma mansoni infection of biomphalaria glabrata and biomphalaria tenagophila in the federal district, brazil]. 19817345560
[biological cycle of paraibatrema inesperata n.g.,n. sp. (trematoda, paramphistomidae) beginning with metacercariae developing biomphalaria tenagophila (d'orbigny, 1835) (mollusca, planorbidae)]. 19817348766
[biological competition between biomphalaria glabrata (say, 1818) and biomphalaria tenagophila (d'orbigny, 1835), in natural breeding areas in the municipality of ourinhos, s.p. (brazil)]. 19807433874
[susceptibility of biomphalaria tenagophila and biomphalaria glabrata from a same region to 2 schistosoma mansoni strains].b. tenagophila snails from ouro branco, mg, showed positivity for s. mansoni, with infection rates of 5%, 10%, (sj strain), and 1% (le strain) using a pool of miracidia. the mollusks were found to be susceptive from the 3rd generation reared in laboratory onwards. the b. tenagophila (ob, mg) when individually exposed to 10 miracidia, showed infection rate of 2% for le strain. b. glabrata snails from gagé, mg, showed a positivity rate of 58% for s. mansoni (le strain), under experimental conditio ...19947569607
[malacological survey of the soledade lake hydrographic basin, in ouro branco (minas gerais, brazil)].a malacological survey was carried out at the soledade lake, in ouro branco, state of minas gerais, for the period 1986-1991. a total amount of 46,579 mollusks was collected, and among them seven species corresponding to five families could be found, as follows: 39,176 specimens of biomphalaria tenagophila; 1,296 b. glabrata; 7 drepanotrema cimex; 2,527 physa sp; 417 lymnaea sp; 92 pomacea hastrum, and 3,064 specimens of melanoides tuberculata (melanniidae = thiaridae) were collected from march/ ...19947569611
[relationship between the pathogenicity of schistosoma mansoni in mice and the susceptibility of the mollusk vector. ii. number of eggs in feces and number and size of granuloma in visceras].the influence of the susceptibility of the vector snails of s. mansoni on the development of the pathogenicity of the worm to the host was studied. off-spring of snails biomphalaria glabrata and biomphalaria tenagophila were used, selected with regard to the susceptibility to s. mansoni of the strains bh and sj trait, respectively. parenteral, f1, f2, f3 and f4 generation cercariae were used for the infection of swiss mice, which were killed eight weeks after infection. the number of eggs in the ...19937997811
[immunologic and parasitologic aspects of biomphalaria tenagophila infected by schistosoma mansoni and other digenea].the behavior of biomphalaria tenagophila amoebocytes was studied in infections produced by schistosoma mansoni and other digenea. the resistance to superinfection was also verified in mixed infections. data on amoebocyte phagocytic activity, on the number of amoebocytes in hemolymph, and on amoebocyte tissue reactions were obtained and electrophoretic and immunodiffusion examinations of the hemolymph were carried out. it was concluded that the snails infected with digenea show resistance to supe ...19937997812
factors conditioning the habitat and the density of biomphalaria tenagophila (orbigny, 1835) in an isolated schistosomiasis focus in rio de janeiro city.the present work was carried out in a watercress garden in alto da boa vista, in the city of rio de janeiro, brazil. the investigation was carried out in two phases. the first one (1985-86) involved the sampling of biomphalaria tenegophila in two areas to determine its relative populational densities. the results showed that the populations presented similar densities and dynamics. the second phase (1988-89) involved the study of the influence of some environmental factors on the establishment o ...19938107608
schistosomiasis mansoni in an area of low transmission. ii. risk factors for infection.risk factors for schistosoma mansoni infection were identified using a 1:1 matched case-control design. the work was conducted in the municipality of pedro de toledo, são paulo state, brazil, an area where the snail host is biomphalaria tenagophila. information on water contact patterns, knowledge, attitudes and practices (kap), socioeconomic and sanitary conditions were obtained by mean of questionnaires. the crude odds ratio estimates and the adjusted odds ratio estimates using the logistic re ...19938115792
morphological characterization of the hemocytes of the pulmonate snail biomphalaria tenagophila.the blood cells of the pulmonate snail biomphalaria tenagophila, an important transmitter of the trematode schistosoma mansoni in brazil, were examined by light and transmission electron microscopy (tem). two hemocyte types were identified: hyalinocytes and granulocytes. hyalinocytes are small young (immature), poorly spreading cells, which have a high nucleocytoplasmic ratio and are especially rich in free ribosomes. they do not appear to contain lysosome-like bodies and represent less than 10% ...19938246758
schistosomiasis mansoni in the region of the triângulo mineiro, state of minas gerais, brazil.in order to reevaluate the possible presence of schistosomiasis mansoni in the triângulo mineiro, one of the areas of the state of minas gerais where this parasite is not commonly found, malacological survey and fecal examinations were undertaken in the region between october 1990 and june 1992. a sample of 7,032 1st grade school children from 29 counties had their feces examined using the kato-katz method. amongst the children examined, two from planura and one from each countie of capinópolis, ...19948524054
degree of host-parasite compatibility between schistosoma mansoni and their intermediate molluscan hosts in brazil.the compatibility of biomphalaria tenagophila, b. straminea and b. glabrata from minas gerais with different strains of schistosoma mansoni was evaluated using the method of frandsen (1979b) in standardized experiments. one hundred and fifty of each species of snail were individually exposed in the laboratory to 50 miracidia of s. mansoni lines le, sj and al. the cercariae from the infected snails were counted and used to calculate tcp/100 indices, which were compared with those of frandsen (197 ...19958524084
development of schistosoma mansoni in biomphalaria tenagophila, biomphalaria straminea and biomphalaria glabrata.a comparative study of the development of schistosoma mansoni during the intra-molluscan phase was made by means of histological sections of biomphalaria tenagophila, b. straminea and b. glabrata from brazil. two hundred snails of each species were individually exposed to 50 miracidia of the s. mansoni, al line. no larvae were observed in the snails fixed 72 h after exposure. in specimens shedding cercariae, 31 days after exposure tissue reactions encapsulating the larvae were seen in b. tenagop ...19958525264
use of isozyme patterns in the identification of biomphalaria tenagophila (d'orbigny, 1835) and b. occidentalis (paraense, 1981) (gastropoda: planorbidae).two sibling species of biomphalaria, b. tenagophila and b. occidentalis were identified using isozyme patterns obtained by horizontal gel electrophoresis. six diagnostic enzymatic loci were identified in digestive gland homogenates. the results enable us to distinguish the species, calculate the nei's coefficient of genetic similarity, and provide a basis for making inferences about the pattern of these two planorbid species colonization and distribution.19958544740
schistosomiasis in a low prevalence area: incomplete urbanization increasing risk of infection in paracambi, rj, brazil.the risk of schistosomiasis infection and heavy infection in the locality of sabugo was evaluated in relation to housing in areas with different urbanization development and to residential supply with snail-infested water. critical sanitary conditions were found in areas of incomplete urbanization, where healthy water supply sources were scarce, and draining of sewage, without previous treatment, was made directly to the water-bodies used for domestic and leisure activities, despite being biomph ...19958551949
[the effect of hemolymph inoculation on the mechanism of defense of biomphalaria tenagophila (orbigny, 1835)].the resistance of b. tenagophila snails to infection by schistosoma mansoni was studied. these snails had been previously inoculated with hemolymph talsen from other snails infected by s. mansoni of sj strain or by other trematodes. the findings suggest that two types--cellular and humoral--are responsible for the resistance to infection by s. mansoni. the cellular response occurred when the snails were inoculated with hemolymph from snails infected by s. mansoni, b. tenagophila inoculate with h ...19958729274
[geographic distribution of planorbidae in santa catarina, brazil].a revision of the malacological surveys carried out in santa catarina, brazil, by the national health foundation in 52 counties of 8 microregions between 1981 and 1994 was made. fifty of them were positive for biomphalaria tenagophila, the only schistosome vector identified in the 94,535 specimens collected. of 1,358 districts, 617 (45.4%) were positive to the vector, varying from 4.3% to 89.4% per municipality. the percentages of water bodies with planorbids varied from 0.2% to 26.3% and, of th ...19968966305
[susceptibility of biomphalaria tenagophila from the paraná and uruguay river basins to schistosoma mansoni].to study the possibility that schistosomiasis might be able to spread into the basins of the paraná and uruguay rivers, 1,711 snails were experimentally exposed to infection with schistosoma mansoni. these snails were laboratory-bred progeny of biomphalaria tenagophila collected from 15 populations in the geographic area under consideration. three strains of s. mansoni were used: bh2, adapted to b. glabrata, and sj and sj2 adapted to b. tenagophila. none of the 543 snails exposed to the bh2 stra ...19979162585
occurrence of biomphalaria tenagophila (d'orbigny, 1835) in the municipality of ilhabela, at the seacoast of são paulo, brazil. 19969163989
[behavior of hemolymph amebocytes from planorbidae in the presence of schistosoma mansoni larvae parasitism, after inoculation of indian ink or fracture of the shell].the behavior of the hemolymph amebocytes of biomphalaria glabrata and biomphalaria tenagophila was studied by means of the infection by the schistosoma mansoni, using inoculation with indian ink and fracture of the shell. a differential count of the amebocytes in the hemolymph was made with special attention given to the types of cell found in each sample. histopathological evaluation of the molluscs exposed to the schistosoma mansoni miracidia and analyses of the amebocytes were made. correlati ...19969201894
occurrence of biomphalaria tenagophila and disappearance of biomphalaria straminea in paracambi, rj, brazil. 19979302412
[relationship between pathogenicity of schistosoma mansoni in mice and the susceptibility of the vector mollusk. iv--infectiousness of miracidia].the infection ability of miracidia of bh and sj strains of s. mansoni, obtained from mice infected with cercariae taken from biomphalaria glabrata and biomphalaria tenagophila, genetically selected for susceptibility is compared with the infection ability of miracidia obtained from mice infected with larvae from non-selected mollusks.19979629726
quantification of the population and phagocytary activity of hemocytes of resistant and susceptible strains of biomphalaria glabrata and biomphalaria tenagophila infected with schistosoma mansoni.among the determinant factors in the resistance and susceptibility of biomphalaria to schistosoma mansoni, hemocytes play an important role. aiming at studying s. mansoni/biomphalaria interactions related to hemocytes, the first step is certainly connected with the standardization of this cell population in uninfected biomphalaria. in this way, quantification of this cell population in hemolymph, as well as its phagocitary capacity, have been determined for the first time. furthermore, using sus ...19979640781
detection of igm antibodies to schistosoma mansoni gut-associated antigens for the study of the dynamics of schistosomiasis transmission in an endemic area with low worm burden.for a period of 2 years, five follow-up measures of prevalence and incidence rates were estimated in a prospective study of s. mansoni infection in a group of schoolchildren who were living in a rural area of the municipality of itariri (são paulo, brazil), where schistosomiasis is transmitted by biomphalaria tenagophila. infection was determined by the examination of three kato-katz stool slides, and the parasitological findings were analyzed in comparison to serological data. in the five surve ...19989876435
schistosoma mansoni: continuous variation in susceptibility of the vector snail of schistosomiasis, biomphalaria tenagophila i. self-fertilization-lineage.mascara, d., kawano, t., magnanelli, a. c., silva, r. p. s., sant' anna, o. a., and morgante, j. s. 1999. schistosoma mansoni: continuous variation in susceptibility of the vector snail of schistosomiasis, biomphalaria tenagophila i. self-fertilization-lineage. experimental parasitology 93, 133-141. artificial selection of biomphalaria tenagophila snails for susceptibility to infection by schistosoma mansoni (brazilian sj strain) was carried out from natural populations. after five self-fertiliz ...199910529355
genetic variation between susceptible and non-susceptible snails to schistosoma infection using random amplified polymorphic dna analysis (rapds).susceptibility of snails to infection by certain trematodes and their suitability as hosts for continued development has been a bewildering problem in host-parasite relationships. the present work emphasizes our interest in snail genetics to determine what genes or gene products are specifically responsible for susceptibility of snails to infection. high molecular weight dna was extracted from both susceptible and non-susceptible snails within the same species biomphalaria tenagophila. rapd was ...199910602543
toxicity of euphorbia milii latex and niclosamide to snails and nontarget aquatic species.the toxicity of euphorbia milii molluscicidal latex and niclosamide (ncl) to target snails (biomphalaria glabrata and biomphalaria tenagophila) and nontarget aquatic organisms is evaluated. planorbidae snails were killed by very low concentrations of lyophilized latex (48-h lc(50), mg/l: b. glabrata, 0.12; b. tenagophila, 0.09; helisoma duryi, 0.10). latex was less toxic (48-h lc(50) or ec(50), mg/l) to oligochaeta (tubifex tubifex, 0.31), planktonic crustacea (daphnia similis, 0.38; c. dubia, 1 ...200010903832
susceptibility of biomphalaria tenagophila and biomphalaria straminea to schistosoma mansoni infection detected by low stringency polymerase chain reaction.in order to determine schistosoma mansoni infection rates in biomphalaria tenagophila and b. straminea, low stringency polymerase chain reaction (ls-pcr) technique was used as a complementary method to light exposure technique. ls-pcr has already been standardized in our laboratory to detect the trematode dna in b. glabrata. higher s. mansoni infection rates were detected using conventional method and ls-pcr. the parasite dna profile was detected in both species after 7-day exposure to miracidia ...200011058941
genetic markers from biomphalaria tenagophila (gastropoda: pulmonata: planorbidae) obtained by the double stringency polymerase chain reaction technique.biomphalaria tenagophila, one of the intermediate hosts of the trematoda schistosoma mansoni, is a simultaneous hermafrodite snail species. in order to analyse the genetic structure of these populations, we performed a double-stringency pcr technique to obtain genetic markers with microsatellites and arbitrary primers in a single reaction.200111313657
[freshwater snails of the campus of manguinhos, oswaldo cruz foundation, rio de janeiro, rj].a survey of freshwater gastropods of the campus of manguinhos, fundação oswaldo cruz, rio de janeiro, was carried out during the last two years aiming to compare the current species with those found at the beginning of this century. among 18 breeding sites in 880,000m2 of the surveyed area, 13 showed the following species: antillorbis nordestensis; biomphalaria glabrata; biomphalaria straminea; lymnaea columella; melanoides tuberculatus; physa cubensis; pomacea glauca and pomacea lineata. notabl ...200111460215
effects of the desiccation on biomphalaria tenagophila (orbigny, 1835) (mollusca) infected by schistosoma mansoni sambon, 1907.specimens of biomphalaria tenagophila exposed to miracidia of schistosoma mansoni were submitted to different desiccation periods as follows: group i: 24 h after exposure, desiccated for 28 days; group ii: after cercariae elimination, desiccated for 7 days; group iii: 21 days after exposure, desiccated for 7 days; group iv: 14 days after exposure, desiccated for 14 days; group v: 7 days after exposure, desiccated for 21 days. from the obtained data it was verified that desiccation was not capabl ...200111500782
freshwater snails and schistosomiasis mansoni in the state of rio de janeiro, brazil: i-- metropolitan mesoregion.in order to elaborate a planorbid chart of the state of rio de janeiro a survey of freshwater gastropods in the metropolitan mesoregion of this state was performed and revealed the occurrence of 20 species: antillorbis nordestensis (lucena, 1954); biomphalaria glabrata (say, 1818); biomphalaria schrammi (crosse, 1864); biomphalaria straminea (dunker, 1848); biomphalaria tenagophila (orbigny, 1835); burnupia sp.; drepanotrema anatinum (orbigny, 1835); drepanotrema cimex (moricand, 1839); drepanot ...200111586447
[search of natural occurrence of xiphidiocercariae (trematoda) in fresh water snails of nine countries from são paulo state, brazil].xiphidiocercariae, aquatic larval stages of some trematodes are considered a potential instrument for biological control of mosquitoes. in this study we evaluated its natural occurrence in campinas region and two places in vale do ribeira (registro and miracatu), são paulo state. snails were obtained from fresh water collections from september 1996 to february 1999. the species collected were lymnaea columella, physa marmorata, biomphalaria tenagophila, biomphalaria sp., drepanotrema cimex, d. l ...200112058671
biomphalaria tenagophila (orbigny, 1835) (mollusca): adaptation to desiccation and susceptibility to infection with schistosoma mansoni sambon, 1907.experiments were carried out to test the susceptibility of biomphalaria tenagophila to the infection with strain sj of schistosoma mansoni in the f1, f2 and non-selected parental generation. the potential adaptation of b. tenagophila to desiccation, in healthy mollusks and those exposed to the larvae of s. mansoni of the f1, f2 and non-selected parental generations was also studied. the presence of mucus and soil, at the shell opening, protected the snails against desiccation, favoring survival. ...200212219110
freshwater snails and schistosomiasis mansoni in the state of rio de janeiro, brazil: ii--centro fluminense mesoregion.during the course of a survey carried out from 2000 to 2001 in the centro fluminense mesoregion of the state of rio de janeiro 22 molluscan species were found. many of the records are new due to the dearth of previous studies. concerning the snail hosts of schistosoma mansoni, the most frequently encountered species was biomphalaria tenagophila, as it occurred in all the surveyed municipalities. there are new records of biomphalaria straminea and biomphalaria peregrina which is regarded as a pot ...200212219122
[schistosomiasis mansoni and distribution of freshwater mollusks in natural bodies of water in niterói, rio de janeiro state, brazil].the authors report on the distribution of freshwater mollusks in niterói, rio de janeiro state, brazil, and notification of schistosomiasis cases in this municipality from 1995 to 2000. all breeding sites favorable to freshwater mollusks were surveyed, showing the following species: antillorbis nordestensis (lucena, 1954), biomphalaria straminea (dunker, 1848), biomphalaria tenagophila (orbigny, 1835), drepanotrema anatinum (orbigny, 1835), lymnaea columella say, 1817, melanoides tuberculatus (m ...200212244379
schistosomiasis mansoni in bananal (state of são paulo, brazil): ii. intermediate hosts.we conducted monthly snail captures in bananal, state of são paulo, brazil, between march 1998 and february 2001, to identify schistosoma mansoni vectors, estimate seasonal population changes, and delimit foci. we also evaluated the impact of improvements in city water supply and basic sanitation facilities. we identified 28,651 vector specimens, 28,438 as biomphalaria tenagophila, 49 of them (0.2%) infected with s. mansoni, and 213 as b. straminea, none of the latter infected. vectors predomina ...200212426592
freshwater snails and schistosomiasis mansoni in the state of rio de janeiro, brazil: iii--baixadas mesoregion.in this paper, the third of a series dealing with the survey of freshwater gastropods of the state of rio de janeiro, the results of collections carried out in the mesoregion baixadas from 2000 to 2002 are presented. twenty-two species, belonging to seven families, were found. as to the snail intermediate hosts of schistosoma mansoni, the most frequent species was biomphalaria tenagophila besides some new findings of biomphalaria straminea. no specimens were found harboring larval forms of s. ma ...200212426593
effect of gamma radiation on the activity of hemocytes and on the course of schistosoma mansoni infection in resistant biomphalaria tenagophila snails.high doses of gamma radiation (10 krad) in biomphalaria tenagophila snails (taim strain), which have been found to be resistant to schistosoma mansoni, were not sufficient to impair their resistance to the parasite. the number of hemocytes, as well as their phagocytic activity, were not affected by irradiation, thus showing resemblance with mammal macrophages, which are resistant to gamma irradiation also.200312700864
silica treatment increases the susceptibility of the cabo frio strain of biomphalaria tenagophila to schistosoma mansoni infection but does not alter the natural resistance of the taim strain.the present work demonstrates that silica treatment represents a suitable in vivo method to evaluate the role of host phagocytic hemocytes in the mechanisms of resistance to parasitic infection. silica inoculation into biomphalaria tenagophila snail induced a significant reduction in the circulating hemolymph granulocytes in both strains tested (taim and cabo frio). the granulocyte reduction was accompanied by a significant increase in the number of circulating dead cells. in b. tenagophila cabo ...200314530969
activity of euphorbia splendens var. hislopii n.e.b. (euphorbiaceae) latex against lymnaea columella (say, 1817) (pulmonata: lymnaeidae), intermediate host of fasciola hepatica, linnaeus, 1758 (trematoda: fasciolidae). 2: limited field-testing.the molluscicidal evaluation of euphorbia splendens var. hislopii (crown of thorns) against lymnaea columella snails, intermediate host of fasciola hepatica, in irrigation ditches of the pisciculture station at universidade federal rural do rio de janeiro, was studied under limited field conditions. an aqueous solution of the latex at 5 mg/l was tested in two irrigation ditches (experimental and control ditches), after initial sampling of the snail population present. twenty-four hours after app ...200414762529
[occurrence of schistosomiasis mansoni vectors on the northern coast of the state of são paulo, brazil].this article comments on the detection of biomphalaria tenagophila (d'orbigny, 1835) infected with schistosoma mansoni sambon, 1907 in caraguatatuba and the introduction of biomphalaria straminea (dunker, 1848) in ilha bela, two municipalities on the northern coast of the state of são paulo, brazil. infected snails were collected from a ditch located in the olaria district. b. straminea and b. tenagophila were living in syntopy in a stream situated in barra velha. such epidemiological findings i ...200414999356
[epidemiology of schistosomiasis mansoni in a low endemic area].we discuss the epidemiological patterns of schistosomiasis mansoni in areas with low transmission in brazil. we define as areas of low endemicity those where the prevalence is less than 10%, the number schistosoma mansoni eggs per gram of feces (epg) is less than 96, and carriers are asymptomatic. data are from the county of pedro de toledo in the ribeira valley (são paulo state) and were collected randomly according to the aggregate pattern of s. mansoni within the hosts. we suggest the replace ...200415042215
dominant character of the molecular marker of a biomphalaria tenagophila strain (mollusca: planorbidae) resistant to schistosoma mansoni.biomphalaria tenagophila population from taim (state of rio grande do sul, brazil) is totally resistant to schistosoma mansoni, and presents a molecular marker of 350 bp by polymerase chain reaction and restriction fragment length polymorphism of the entire rdna internal transcriber spacer. the scope of this work was to determine the heritage pattern of this marker. a series of cross-breedings between b. tenagophila from taim (resistant) and b. tenagophila from joinville, state of santa catarina ...200415057353
biomphalaria tenagophila: genetic variability within intermediate snail hosts susceptible and resistant to schistosoma mansoni infection.dna analysis by molecular techniques has significantly expanded the perspectives of the study and understanding of genetic variability in molluscs that are vectors of schistosomiasis. in the present study, the genetic variability of susceptible and resistant b. lenagophila strains to s. mansoni infection was investigated using amplification of their genomic dna by rapd-pcr. the products were analyzed by page and stained with silver. the results showed polymorphism between tested strains with fou ...200415071826
freshwater snails and schistosomiasis mansoni in the state of rio de janeiro, brazil: iv - sul fluminense mesoregion.in this paper, the forth of a series dealing with the survey of freshwater gastropods of the state of rio de janeiro, the results of collections carried out in the sul fluminense mesoregion from 2000 to 2002 are presented and revealed the occurrence of 18 species: antillorbis nordestensis; biomphalaria glabrata; biomphalaria peregrina; biomphalaria straminea; biomphalaria tenagophila; drepanotrema anatinum; drepanotrema cimex; drepanotrema lucidum; ferrissia sp.; gundlachia ticaga; gundlachia sp ...200415273799
polymerase chain reaction and restriction fragment length polymorphism analysis of the its2 region for differentiation of brazilian biomphalaria intermediate hosts of the schistosoma mansoni.we sequenced the internal transcribed spacer 2 of the ribosomal dna (its2-dnar) from the three schistosoma mansoni intermediate hosts in brazil: biomphalaria glabrata, biomphalaria tenagophila and biomphalaria straminea. analysis of a restriction map from those sequences allowed us to select putative restriction enzymes able to identify the snail species under study. four restriction enzymes were used and hpaii provided simple species-specific profiles easily visualized in polyacrylamide gels. t ...200415334271
freshwater snails and schistosomiasis mansoni in the state of rio de janeiro, brazil: v -- norte fluminense mesoregion.in this paper, the fifth of a series dealing with the survey of freshwater gastropods of the state of rio de janeiro, the results of collections carried out in the norte fluminense mesoregion from 2002 to 2003 are presented and revealed the occurrence of 19 species: antillorbis nordestensis; burnupia sp.; biomphalaria tenagophila; drepanotrema anatinum; drepanotrema cimex; drepanotrema depressissimum; drepanotrema lucidum; ferrissia sp.; gundlachia ticaga; gundlachia sp.; heleobia sp.; hebetancy ...200415486644
biomphalaria tenagophila/schistosoma mansoni interaction: premises for a new approach to biological control of schistosomiasis.biomphalaria tenagophila is very important for schistosomiasis transmission in brazil. however its mechanisms of interaction with schistosoma mansoni are still scantly studied. since this snail displays strains highly susceptible or completely resistant to the parasite infection, the knowledge of that would be a useful tool to understand the mechanism of snail resistance. particularly, the taim strain consistently shows absolute resistance against the trematode, and this resistance is a dominant ...200415486646
a neotropical snail host of schistosoma mansoni introduced into africa and consequences for the schistosomiasis transmission biomphalaria tenagophila in kinshasa (democratic republic of congo).malacological surveys carried out in the early 1970s in water bodies of the kinshasa area, lower zaire (democratic republic of congo), showed the appearance of a biomphalaria species which was identified as biomphalaria camerunensis. in 1976, other surveys confirmed the presence of the species in several sites and showed numerous infected snails with schistosoma mansoni, demonstrating for the first time an active transmission of the parasite responsible of the intestinal schistosomiasis in this ...200415652333
biomphalaria tenagophila: dominant character of the resistance to schistosoma mansoni in descendants of crossbreedings between resistant (taim, rs) and susceptible (joinville, sc) strains.the aim of the present work was to study parasitological, molecular, and genetic aspects in descendants of crossbreedings between a totally resistant biomphalaria tenagophila strain (taim, rs) and another one highly susceptible (joinville, sc) to schistosoma mansoni. descendants f1 and f2 were submitted to s. mansoni infection (le strain). the susceptibility rates for individuals from group f1 were 0 to 0.6%, and from group f2 was 7.2%. the susceptible individuals from group f2 discharged a lowe ...200515867958
influence of saccharose on the development of cercariae from schistosoma mansoni strains bh and sj.the development of cercariae from schistosoma mansoni strains bh and sj in biomphalaria glabrata and biomphalaria tenagophila treated with saccharose was studied. the molluscs were maintained in dechlorinated tap water containing 0.01% saccharose. after one week of treatment with saccharose, b. glabrata and b. tenagophila were exposed to ten s. mansoni miracidia, from bh and sj strains respectively. control snails of both species were maintained in dechlorinated tap water without saccharose and ...200516025902
[genetic selection of biomphalaria glabrata and biomphalaria tenagophila seeking the alteration of the susceptibility and resistance to schistosoma mansoni].generations of biomphalaria glabrata and biomphalaria tenagophila selected genetically for resistance and susceptibility to schistosoma mansoni of strains bh and sj were used in a study of the trematode adaptation to the intermediate host. descendants of the planorbids were obtained by self-fertilization of the mollusks that became susceptible or resistant after exposure to the miracidia of schistosoma mansoni. for biomphalaria glabrata they were obtained from the following generations: parental ...200516172752
[geographic distribution of schistosoma mansoni transmitter snail species in state of são paulo].a thorough knowledge of the geographic distribution of schistosoma mansoni vector snails is indispensable for the control of schistosoma mansoni schistosomiasis and its epidemiologic surveillance. from the water masses of the state of são paulo (brazil) 8,771 lots of snails--total of 108,244 individuals of the genus biomphalaria--were captured between 1982 and 2002. these specimens are now part of the malacological collection of (superintendência de controle de endemias--são paulo). according to ...200516172761
resistance to schistosoma mansoni by transplantation of apo biomphalaria tenagophila.transplantation of the haematopoietic organ from biomphalaria tenagophila (taim strain, rs, brazil), resistant to schistosoma mansoni, to a highly susceptible strain (cabo frio, rj, brazil) of the same species, showed in the recipient snails resistance against the trematode, when a successful transplant occurred. the success of transplantation could be confirmed by a typical molecular marker of the taim strain in haemocytes of the recipients (350 bp detected by pcr-rflp). the recipient snails wh ...200616629706
compatibility of biomphalaria tenagophila with schistosoma mansoni: a study of homologous plasma transference.this study aims to investigate the importance of the serum factors present in the plasma of resistant biomphalaria tenagophila snails, when transferred to susceptible conspecific. susceptible b. tenagophila (cf) received plasma from resistant b. tenagophila (taim), and both were later infected with schistosoma mansoni. we noticed that the plasma transfer showed an increase on the resistance of susceptible snails of about 86% when compared to the non-immunized group (p < 0.001).200616699720
behavior of angiostrongylus costaricensis in planorbids.some terrestrial mollusks are natural hosts of angiostrongylus costaricensis. in the laboratory, this nematode can be maintained in certain planorbids, which are aquatic mollusks and intermediate hosts of schistosoma mansoni. mollusks can be infected with angiostrongylus costaricensis by ingestion of or active penetration by the first-stage larvae. in this work we assessed the ability of biomphalaria glabrata to attract first-stage larvae of a. costaricensis. movement of the nematode larvae towa ...200616710513
effect of angiostrongylus vasorum infection on biomphalaria tenagophila susceptibility to schistosoma mansoni.concomitant infection with different parasites may be a helpful laboratorial strategy leading to the better understanding of the mechanisms used by the internal defense system (ids) of gastropoda to deal with helminth infection, such as schistosoma mansoni. this work reports the effect of co-infection of angiostrongylus vasorum and s. mansoni in hemocyte activity and in the outcome of infection. the simultaneous infection resulted in an increase of snail susceptibility to s. mansoni. in contrast ...200616750811
differential lectin labelling of circulating hemocytes from biomphalaria glabrata and biomphalaria tenagophila resistant or susceptible to schistosoma mansoni infection.lectins/carbohydrate binding can be involved in the schistosoma mansoni recognition and activation of the biomphalaria hemocytes. therefore, expression of lectin ligands on biomphalaria hemocytes would be associated with snail resistance against s. mansoni infection. to test this hypothesis, circulating hemocytes were isolated from b. glabrata bh (snail strain highy susceptible to s. mansoni), b. tenagophila cabo frio (moderate susceptibility), and b. tenagophila taim (completely resistant strai ...200617308768
intermediate and definitive hosts of schistosoma mansoni in corrientes province, argentina.corrientes province is located in the humid subtropical region of argentina northeast on the left riverbank of paraná river in the border with the south of brazil. this is a region without schistosomiasis but planorbid and rodents that would serve as host of the life cycle of schistosoma mansoni inhabit here. the objective of this work is to know the role of rodent as definitive host of schistosomiasis. biomphalaria tenagophila (4 to 8 mm ø) from maloyas, exposed each to 10 miracidia of sj2 stra ...200617308774
biomphalaria tenagophila: dynamics of populations of resistant and susceptible strains to schistosoma mansoni, with or without pressure of the parasite.resistant (taim, rs) and susceptible albino (joinville, sc) biomphalaria tenagophila populations were kept together, at different proportions, throughout a 18-month-period. some of the snail groups were submitted to schistosoma mansoni infection. the targets of this study were (a) to analyze the populational dynamics among resistant and susceptible individuals to s. mansoni; (b) to study the resistance phenotype in descendants of cross-breeding; (c) to observe whether the parasite could exert an ...200617308777
biomphalaria tenagophila potencial vector of schistosoma mansoni in the paraná river basin (argentina and paraguay).susceptibility and compatibility experiments were carried out with 700 biomphalaria tenagophila from the paraná river basin exposed to infection with schistosoma mansoni. individual infection was performed with 10 miracidia of sj2 strain from the paraiba valley (brazil) originally infective to b. tenagophila. these snails were laboratory-breed progeny of b. tenagophila collected from six localities of argentina and one from paraguay. from argentina: rincón de vences (7%) and posadas (11%) became ...200717426884
proteome analysis of the cardiac and pericardial tissue of biomphalaria tenagophila populations susceptible and resistant to schistosoma mansoni infection.for a better comprehension of the parasite-host interaction, proteins expressed by the cardiac and pericardial tissues were compared between susceptible (cabo frio) and resistant (taim) biomphalaria tenagophila populations, challenged (c) and non-challenged (nc) with schistosoma mansoni. proteins were separated by two-dimensional gel electrophoresis (2de) and stained with coomassie blue. a total of 146 and 135 spots were observed in cabo frio (cfnc) and in taim (tnc) non-challenged populations, ...200818262165
transmission control of schistosomiasis mansoni by introduction of a resistant strain of biomphalaria tenagophila in areas where transmission is maintained by this species.biomphalaria tenagophila taim lineage has proved to be consistently resistant to schistosoma mansoni. several published works have shown that this resistance is due to the innate defence system of that strain, and in cross-breedings with susceptible strains the taim lineage presents dominant character. these findings led to the hypothesis that, introducing this strain in areas where transmission of schistosomiasis is maintained by this species, the introduced lineage would perform cross-breeding ...200818598664
establishment of biomphalaria tenagophila snails in europe. 200818976582
participation of cell-free haemolymph of biomphalaria tenagophila in the defence mechanism against schistosoma mansoni sporocysts.biomphalaria tenagophila of taim strain is able to completely destroy schistosoma mansoni sporocyst few hours after parasite penetration, although the mechanism is still not well known. in this experimental work we show that passive transference of cell-free haemolymph, especially from b. tenagophila taim, resulted in higher resistance of b. tenagophila cabo frio to s. mansoni infection. this effect was demonstrated in vivo, by the reduction in the infection rate, and the significantly lower pro ...200819067842
flow cytometry analysis of the circulating haemocytes from biomphalaria glabrata and biomphalaria tenagophila following schistosoma mansoni infection.aiming to further characterize the haemocyte subsets in biomphalaria snails, we have performed a detailed flow cytometric analysis of whole haemolymph cellular components using a multiparametric dual colour labelling procedure. ethidium bromide/acridine orange fluorescence features were used to first select viable haemocytes followed by flow cytometric morphometric analysis based on the laser scatter properties (forward scatter-fsc and side scatter-ssc). our findings demonstrated that b. glabrat ...200919126270
schistosoma mansoni and other larval trematodes in biomphalaria tenagophila (planorbidae) from guarulhos, são paulo state, brazil.a total of 909 biomphalaria tenagophila were collected from two areas in guarulhos (metropolitan area of são paulo, são paulo state, brazil) to assess larval trematode infections. in all collection sites, only this species was found and 183 (20.13%) harbored trematode infections. in these collections, four morphologically distinguishable types of cercariae were identified by confocal microscopy. xiphidiocercaria (cercaria lutzi) was the most common type of cercaria recovered, contributing 76.5% ...200919390735
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