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[serodiagnosis of celery mosaic virus in agar-gel double-diffusion test]. 19724633943
a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family.a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ...200111402861
potyviruses, novel and known, in cultivated and wild species of the family apiaceae in australia.three potyviruses were identified by gene sequencing and found to be widespread in species of apiaceae in australia. only celery mosaic virus was found in celery crops and in one of 180 specimens of feral carrot ( daucus carota). another related but distinct novel potyvirus, carrot virus y, was the only virus found in carrot crops and all except one feral carrot. a more distantly related novel potyvirus, apium virus y, was found in plants of sea celery ( apium prostratum), cultivated parsley ( p ...200212376749
biological characterization and complete genomic sequence of apium virus y infecting celery.a celery isolate of apium virus y (apvy-ce) from diseased plants in a commercial field in california was characterized. the experimental host range of the virus included 13 plant species in the families apiaceae, chenopodiaceae and solanaceae. almost all infected plant species showed foliar chlorosis and distortion or severe stunting and systemic chlorosis. apvy-ce was transmitted to all 10 host species in the apiaceae by green peach aphids. it reacted with the potyvirus group antibody and celer ...201120833213
complete genome sequence of celery mosaic virus and its relationship to other members of the genus potyvirus.the complete genomic sequence of celery mosaic virus (cemv) was found to be 9999 nucleotides in length, excluding the 3' poly(a) tail. the genome contains a single large open reading frame encoding a polyprotein of 3181 amino acids. its genomic organization is typical of potyviruses and contains conserved motifs found in members of the genus potyvirus. pairwise comparison of the polyprotein sequences shows that cemv shares 39.0-71.9% sequence identity with other members of the genus potyvirus. p ...201121347842
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