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schineria larvae gen. nov., sp. nov., isolated from the 1st and 2nd larval stages of wohlfahrtia magnifica (diptera: sarcophagidae).four bacterial strains were isolated from the fly larvae of an obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae). these isolates were characterized by a polyphasic approach and represent a new lineage of gamma-proteobacteria as their closest relative is xylella fastidiosa (87.1% 16s rdna similarity). the four strains are identical at the 16s rdna level, the level of similarity between them, based on dna-dna hybridization, is high (97.8-102.5%) and they are similar in their p ...200111321085
analysis of the bacterial community inhabiting an aerobic thermophilic sequencing batch reactor (at-sbr) treating swine waste.the microflora of a self-heating aerobic thermophilic sequencing batch reactor (at-sbr) treating swine waste was investigated by a combination of culture and culture-independent techniques. the temperature increased quickly in the first hours of the treatment cycles and values up to 72 degrees c were reached. denaturing gradient gel electrophoresis of the pcr-amplified v3 region of 16s rdna (pcr-dgge) revealed important changes in the bacterial community during 3-day cycles. a clone library was ...200415549286
proposal to replace the illegitimate genus name schineria toth et al. 2001 with the genus name ignatzschineria gen. nov. and to replace the illegitimate combination schineria larvae toth et al. 2001 with ignatzschineria larvae comb. nov.the prokaryotic, generic name schineria tóth et al. 2001 is illegitimate owing to the prior existence of the name schineria for a genus within the diptera [principle 2, rule 51b(4) of the bacteriological code (1990 revision)]. therefore, a new genus name, ignatzschineria gen. nov., is proposed for this taxon. as a result, a new combination is required for the type species, ignatzschineria larvae comb. nov., to replace the illegitimate combination schineria larvae tóth et al. 2001.200717220462
wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from wohlfahrtia magnifica (diptera: sarcophagidae).new gammaproteobacteria were isolated from 3rd stage fly larvae of the parasitic fly wohlfahrtia magnifica. phylogenetic analysis of the new isolates showed that these bacteria belong to a distinct lineage close to ignatzschineria larvae, which was originally isolated from the same species of fly. the low similarity values in 16s rrna gene sequences (93.8-94.8 %), and differences in fatty acid profiles, riboprint patterns, maldi-tof mass spectra of cell extracts, and physiological and biochemica ...200818398205
ignatzschineria indica sp. nov. and ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (diptera: sarcophagidae).two gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains ffa1(t) and ffa3(t), and belonging to the class gammaproteobacteria were isolated from the gastrointestinal tract of adult flesh flies (diptera: sarcophagidae). phylogenetic analysis of 16s rrna gene sequence data placed these two strains within the genus ignatzschineria with similarities of 98.6 % (ffa1(t)) and 99.35 % (ffa3(t)) to ignatzschineria larvae l1/68(t). the level of gene sequence similarity betwe ...201020584814
comparison of bacterial diversity in wheat bran and in the gut of larvae and newly emerged adult of musca domestica (diptera: muscidae) by use of ethidium monoazide reveals bacterial colonization.the objective of the current study is to investigate the bacterial colonization within the gut of the house fly, musca domestica l. (diptera: muscidae), at the larval stage and the bacterial community of the gut of the house fly at the newly emerged adult stage. after using ethidium monoazide to inhibit recovery of nucleic acids from dead bacteria, three polymerase chain reaction (pcr)-amplified 16s rdna libraries from wheat bran, larvae, and newly emerged adults was constructed, analyzed, and c ...201021061987
novel genotypes of anaplasma bovis, "candidatus midichloria" sp. and ignatzschineria sp. in the rocky mountain wood tick, dermacentor andersoni.bovine anaplasmosis, caused by anaplasma marginale, is a vector-borne disease that is enzootic in many parts of the usa. although dermacentor andersoni, a major vector of a. marginale, occurs in canada, the canadian cattle herds are currently considered free of bovine anaplasmosis. there have been two outbreaks of the disease in the province of saskatchewan, but these have been linked to the importation of infected animals. however, the distribution of bovine anaplasmosis may alter with range ex ...201121334146
16s rdna-based phylogeny of non-symbiotic bacteria of entorno-pathogenic nematodes from infected insect cadavers.using 16s rdna gene sequencing technique, three different species of non-symbiotic bacteria of entomopatho-genic nematodes (epns) (steinernema sp. and heterorhabditis sp.) were isolated and identified from infected insect cadavers {galleria mellonella larvae) after 48-hour post infections. sequence similarity analysis revealed that the strains srk3, srk4 and srk5 belong to ochrobactrum cytisi, schineria larvae and ochrobactrum anthropi, respectively. the isolates o. anthropi and s. larvae were f ...201121802047
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