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schineria larvae gen. nov., sp. nov., isolated from the 1st and 2nd larval stages of wohlfahrtia magnifica (diptera: sarcophagidae).four bacterial strains were isolated from the fly larvae of an obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae). these isolates were characterized by a polyphasic approach and represent a new lineage of gamma-proteobacteria as their closest relative is xylella fastidiosa (87.1% 16s rdna similarity). the four strains are identical at the 16s rdna level, the level of similarity between them, based on dna-dna hybridization, is high (97.8-102.5%) and they are similar in their p ...200111321085
analysis of the bacterial community inhabiting an aerobic thermophilic sequencing batch reactor (at-sbr) treating swine waste.the microflora of a self-heating aerobic thermophilic sequencing batch reactor (at-sbr) treating swine waste was investigated by a combination of culture and culture-independent techniques. the temperature increased quickly in the first hours of the treatment cycles and values up to 72 degrees c were reached. denaturing gradient gel electrophoresis of the pcr-amplified v3 region of 16s rdna (pcr-dgge) revealed important changes in the bacterial community during 3-day cycles. a clone library was ...200415549286
bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae).wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ...200616382282
proposal to replace the illegitimate genus name schineria toth et al. 2001 with the genus name ignatzschineria gen. nov. and to replace the illegitimate combination schineria larvae toth et al. 2001 with ignatzschineria larvae comb. nov.the prokaryotic, generic name schineria tóth et al. 2001 is illegitimate owing to the prior existence of the name schineria for a genus within the diptera [principle 2, rule 51b(4) of the bacteriological code (1990 revision)]. therefore, a new genus name, ignatzschineria gen. nov., is proposed for this taxon. as a result, a new combination is required for the type species, ignatzschineria larvae comb. nov., to replace the illegitimate combination schineria larvae tóth et al. 2001.200717220462
human infection with schineria iarvae. 200717561570
isolation of schineria sp. from a man. 200717561571
wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from wohlfahrtia magnifica (diptera: sarcophagidae).new gammaproteobacteria were isolated from 3rd stage fly larvae of the parasitic fly wohlfahrtia magnifica. phylogenetic analysis of the new isolates showed that these bacteria belong to a distinct lineage close to ignatzschineria larvae, which was originally isolated from the same species of fly. the low similarity values in 16s rrna gene sequences (93.8-94.8 %), and differences in fatty acid profiles, riboprint patterns, maldi-tof mass spectra of cell extracts, and physiological and biochemica ...200818398205
bacterial taxa associated with the hematophagous mite dermanyssus gallinae detected by 16s rrna pcr amplification and ttge fingerprinting.dermanyssus gallinae (arthropoda, mesostigmata) is suspected to be involved in the transmission of a wide variety of pathogens, but nothing is known about its associated non-pathogenic bacterial community. to address this question, we examined the composition of bacterial communities in d. gallinae collected from standard poultry farms in brittany, france. genetic fingerprints of bacterial communities were generated by temporal temperature gradient gel electrophoresis (ttge) separation of indivi ...200919027065
endosymbiotic bacteria living inside the poultry red mite (dermanyssus gallinae).this study investigated the endosymbiotic bacteria living inside the poultry red mite collected from five samples of one commercial farm from the uk and 16 farms from france using genus-specific pcr, pcr-ttge and dna sequencing. endosymbiotic bacteria are intracellular obligate organisms that can cause several phenotypic and reproductive anomalies to their host and they are found widespread living inside arthropods. the farm sampled from the uk was positive for bacteria of the genera cardinium s ...200919145467
ignatzschineria indica sp. nov. and ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (diptera: sarcophagidae).two gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains ffa1(t) and ffa3(t), and belonging to the class gammaproteobacteria were isolated from the gastrointestinal tract of adult flesh flies (diptera: sarcophagidae). phylogenetic analysis of 16s rrna gene sequence data placed these two strains within the genus ignatzschineria with similarities of 98.6 % (ffa1(t)) and 99.35 % (ffa3(t)) to ignatzschineria larvae l1/68(t). the level of gene sequence similarity betwe ...201020584814
bacterial community analysis of swine manure treated with autothermal thermophilic aerobic digestion.due to the environmental problems associated with disposal of livestock sludge, many stabilization studies emphasizing on the sludge volume reduction were performed. however, little is known about the microbial risk present in sludge and its stabilized products. this study microbiologically explored the effects of anaerobic lagoon fermentation (alf) and autothermal thermophilic aerobic digestion (atad) on pathogen-related risk of raw swine manure by using culture-independent 16s rdna cloning and ...201020922382
comparison of bacterial diversity in wheat bran and in the gut of larvae and newly emerged adult of musca domestica (diptera: muscidae) by use of ethidium monoazide reveals bacterial colonization.the objective of the current study is to investigate the bacterial colonization within the gut of the house fly, musca domestica l. (diptera: muscidae), at the larval stage and the bacterial community of the gut of the house fly at the newly emerged adult stage. after using ethidium monoazide to inhibit recovery of nucleic acids from dead bacteria, three polymerase chain reaction (pcr)-amplified 16s rdna libraries from wheat bran, larvae, and newly emerged adults was constructed, analyzed, and c ...201021061987
novel genotypes of anaplasma bovis, "candidatus midichloria" sp. and ignatzschineria sp. in the rocky mountain wood tick, dermacentor andersoni.bovine anaplasmosis, caused by anaplasma marginale, is a vector-borne disease that is enzootic in many parts of the usa. although dermacentor andersoni, a major vector of a. marginale, occurs in canada, the canadian cattle herds are currently considered free of bovine anaplasmosis. there have been two outbreaks of the disease in the province of saskatchewan, but these have been linked to the importation of infected animals. however, the distribution of bovine anaplasmosis may alter with range ex ...201121334146
16s rdna-based phylogeny of non-symbiotic bacteria of entorno-pathogenic nematodes from infected insect cadavers.using 16s rdna gene sequencing technique, three different species of non-symbiotic bacteria of entomopatho-genic nematodes (epns) (steinernema sp. and heterorhabditis sp.) were isolated and identified from infected insect cadavers {galleria mellonella larvae) after 48-hour post infections. sequence similarity analysis revealed that the strains srk3, srk4 and srk5 belong to ochrobactrum cytisi, schineria larvae and ochrobactrum anthropi, respectively. the isolates o. anthropi and s. larvae were f ...201121802047
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