| a classification of the tobamoviruses based on comparisons among their 126k proteins. | the products of partial proteolysis of the mr 126,000 in vitro translation products of the rna of eight tobamoviruses were separated by sds-polyacrylamide gel electrophoresis. the peptide patterns obtained were compared using a computer program designed to establish phylogenetic relationships. the resulting most-parsimonious phylogenetic trees grouped the tobamoviruses into clusters i (tobacco mosaic virus, tomato mosaic virus, tobacco mild green mosaic virus, pepper mild mottle virus) and ii (s ... | 1990 | 2230728 |
| taxonomy of cucurbit-infecting tobamoviruses as determined by serological and molecular hybridization analyses. | a number of cucurbit-infecting tobamoviruses have been reported in the past but there is confusion about their identity and relationships. cucumber viruses 3 (cv3) and 4 (cv4) were originally described in the united kingdom whereas the watermelon (w) and cucumber (c) 'isolates' of cucumber green mottle mosaic virus (cgmmv) came from japan. the results of serological studies and rna-cdna analyses have shown that cv3, cv4 and cgmmv-w are very closely related, whereas cgmmv-c is quite different. it ... | 1986 | 3583663 |
| the genome structure of kyuri green mottle mosaic tobamovirus and its comparison with that of cucumber green mottle mosaic tobamovirus. | the genome of the y strain of kyuri green mottle mosaic virus (kgmmv-y) has been completely sequenced. its genomic structure and sequence show it to be a typical tobamovirus, that is closest to, but distinct from, that of cucumber green mottle mosaic tobamovirus (cgmmv). the genomic sequence of kgmmv-y was compared in detail with that of the sh strain of cgmmv. the sequences of their 5'- and 3'-untranslated regions were 74% and 63% identical. the amino acid sequences of the shorter and longer (r ... | 2000 | 10948983 |
| zucchini green mottle mosaic virus is a new tobamovirus; comparison of its coat protein gene with that of kyuri green mottle mosaic virus. | a novel virus we call zucchini green mottle mosaic virus (zgmmv) was isolated from zucchini squash and its properties were determined. the size and shape of its virions, and other properties suggest that the virus is a tobamovirus. the coat protein (cp) genes of zgmmv and kyuri green mottle mosaic virus (kgmmv), which also infects zucchini squash plants, were cloned and their nucleotides sequences were determined. the cp genes of zgmmv and kgmmv are composed of 161 amino acid residues, and they ... | 2000 | 11205120 |
| completion of nucleotide sequence and generation of highly infectious transcripts to cucurbits from full-length cdna clone of kyuri green mottle mosaic virus. | the nucleotide sequence of the genome of the type strain of kyuri green mottle mosaic virus (kgmmv-c1) has been completely determined. the genome structure and sequence of the virus were compared to those of yodo strain of kgmmv (kgmmv-y). the genome of kgmmv-c1 is 6,514 nucleotides long consisting of 5' and 3' nontranslated regions (ntrs) and four open reading frames coding for 131 kda and 189 kda viral replicases, 28 kda movement protein and 17 kda coat protein. the nucleotide and amino acid s ... | 2001 | 11765913 |
| plant virus cdna chip hybridization for detection and differentiation of four cucurbit-infecting tobamoviruses. | a plant virus cdna chip was developed by using viral cdna clones and microarray technology. the cdna chip was designed for detection and differentiation of the four species of selected cucurbit-infecting tobamoviruses [target viruses: cucumber green mottle mosaic virus (cgmmv); cucumber fruit mottle mosaic virus (cfmmv); kyuri green mottle mosaic virus (kgmmv); and zucchini green mottle mosaic virus (zgmmv)]. the chip consisted of cdna clones of the four cucurbit-infecting tobamoviruses, two tar ... | 2003 | 12757916 |
| molecular analysis of quasispecies of kyuri green mottle mosaic virus. | the population diversity of progeny viruses of kyuri green mottle mosaic virus (kgmmv) in consecutive serial passages in two systemic hosts, zucchini squash and cucumber plants, established from genetically identical viral rna, was examined in this study. an initial plant was inoculated with in vitro transcripts from a full-length cdna clone of kgmmv. the initial viral population (passage 0) was transferred five times in parallel populations in the same hosts species for analysis of progenies of ... | 2005 | 15845267 |
| molecular evidence supporting the confirmation of maracuja mosaic virus as a species of the genus tobamovirus and production of an infectious cdna transcript. | the complete genome sequence of maracuja mosaic virus (marmv) was determined and analyzed. the full marmv genome consisted of 6794 nucleotides, and this is the largest genome size among known tobamoviruses. the marmv genome rna contained four open reading frames (orfs) coding for proteins of m(r) 126, 181, 34 and 18 kda from the 5' to 3' end, respectively. the lengths of the 5' nontranslated region (ntr) and the 3' ntr were 54 and 177 nucleotides, respectively. phylogenetic tree analysis reveale ... | 2006 | 16862384 |
| genome structure and production of biologically active in vitro transcripts of cucurbit-infecting zucchini green mottle mosaic virus. | abstract the complete nucleotide sequence of the zucchini green mottle mosaic virus (zgmmv), a new member of the genus tobamovirus, has been determined. the genome of zgmmv is 6,513 nucleotides long and contains four open reading frames coding for proteins of 131, 189, 28, and 17 kda from the 5' to 3' end, respectively. the 5'- and 3'-non-translated regions consist of 59 and 163 residues, respectively. the sequences of the viral proteins exhibit high identity to the proteins of the members of th ... | 2002 | 18943088 |
| biological and molecular characterization of a new cucurbit-infecting tobamovirus. | abstract an uncharacterized virus was isolated from greenhouse-grown cucumber plants. biological and serological data described in the present study indicated that the virus belonged in the genus tobamovirus. the host range of the virus included several plant species within the family cucurbitaceae. the virus designated cucumber fruit mottle mosaic virus (cfmmv) causes severe mottling or mosaic on cucumber fruits, and its fast spread within greenhouses could lead to significant economic losses i ... | 2001 | 18943945 |
| [complete genomic sequence of a watermelon isolate of cucumber green mottle mosaic virus in northern china]. | the complete genomic sequence of a watermelon isolate of cucumber green mottle mosaic virus (cgmmv-ln) in liaoning province was determined and compared with other cucurbit-infecting tobamoviruses. the genomic rna of cgmmv-ln comprised 6422 nt, and 5'- and 3'- noncoding regions consisted of 59 nt and 175 nt, respectively. the encoded four proteins were two replicase proteins of 186 kd and 129 kd, move protein of 29 kd and coat protein of 17.4 kd. the alignment results of complete nucleotide seque ... | 2009 | 19437890 |
| complete nucleotide sequences and construction of full-length infectious cdna clones of cucumber green mottle mosaic virus (cgmmv) in a versatile newly developed binary vector including both 35s and t7 promoters. | seed-transmitted viruses have caused significant damage to watermelon crops in korea in recent years, with cucumber green mottle mosaic virus (cgmmv) infection widespread as a result of infected seed lots. to determine the likely origin of cgmmv infection, we collected cgmmv isolates from watermelon and melon fields and generated full-length infectious cdna clones. the full-length cdnas were cloned into newly constructed binary vector pjy, which includes both the 35s and t7 promoters for versati ... | 2017 | 27913980 |
| characterisation and diagnosis of frangipani mosaic virus from india. | frangipani mosaic virus (frmv) is known to infect frangipani tree (plumeria rubra f. acutifolia) in india but the virus has not been characterized at genomic level and diagnosis is not available. in the present study, an isolate of frmv (frmv-ind-1) showing greenish mosaic and vein-banding symptoms in p. rubra f. acutifolia in new delhi was characterized based on host reactions, serology and genome sequence. the virus isolate induced local symptoms on several new experimental host species: capsi ... | 2015 | 26239043 |
| simultaneous multiplex pcr detection of seven cucurbit-infecting viruses. | two multiplex polymerase chain reaction (pcr) systems using dual priming oligonucleotide (dpo) primers were developed for the simultaneous detection of seven cucurbit-infecting viruses. one system allows for the detection of papaya ringspot virus, watermelon mosaic virus, and zucchini yellow mosaic virus, whereas the other permits the detection of cucumber green mottle mosaic virus, cucumber fruit mottle mosaic virus, kyuri green mottle mosaic virus, and zucchini green mottle mosaic virus. viral ... | 2014 | 24937806 |