| effect of ph change on the performance and microbial community of enhanced biological phosphate removal process. | an acetate-rich wastewater, containing 170 mg/l of total organic carbon (toc), 13 mg/l of n, and 15 mg/l of p, was treated using the enhanced biological phosphate removal (ebpr) process operated in a sequencing batch reactor. a slight change of ph of the mixed liquor from 7.0 to 6.5 led to a complete loss of phosphate-removing capability and a drastic change of microbial populations. the process steadily removed 94% of toc and 99.9% of p from the wastewater at ph 7.0, but only 93% toc and 17% of ... | 2005 | 15962340 |
| identification and occurrence of tetrad-forming alphaproteobacteria in anaerobic-aerobic activated sludge processes. | in an acetate-fed anaerobic-aerobic membrane bioreactor, a deteriorated enhanced biological phosphorus removal (ebpr) community was developed (as determined based on the chemical profiles of organic substrate, soluble phosphate, and intracellular carbohydrate and polyhydroxyalkanote (pha) concentrations). microscopic observations revealed the dominance of tetrad-forming organisms (tfos), of which the majority stained positively for pha under anaerobic conditions. fluorescence in situ hybridizati ... | 2004 | 15528660 |
| structure and functional analysis of the microbial community in an aerobic: anaerobic sequencing batch reactor (sbr) with no phosphorus removal. | the bacterial community of an aerobic:anaerobic non-p removing sbr biomass fed a mixture of acetate and glucose was analysed using several 16s rrna based methods. populations responsible for anaerobic glucose and acetate assimilation were determined with fluorescent in situ hybridization (fish) in combination with microautoradiography (fish/mar). at 'steady state' this community consisted of alpha-proteobacteria (26%) and gamma-proteobacteria (14%), mainly appearing as large cocci in tetrads (i. ... | 2001 | 11876367 |
| pyrosequencing-based assessment of bacterial community structure along different management types in german forest and grassland soils. | soil bacteria are important drivers for nearly all biogeochemical cycles in terrestrial ecosystems and participate in most nutrient transformations in soil. in contrast to the importance of soil bacteria for ecosystem functioning, we understand little how different management types affect the soil bacterial community composition. | 2011 | 21359220 |
| filamentous members of cluster iii defluviicoccus have the in situ phenotype expected of a glycogen-accumulating organism in activated sludge. | the in situ ecophysiology of alphaproteobacterial filamentous cluster iii defluviicoccus present in enhanced biological phosphorus removal (ebpr)-activated sludge systems was evaluated using fish-mar and histochemical staining methods. these organisms, sharing the nostocoida limicola morphotype, are known to be responsible for serious episodes of activated sludge bulking. the data presented here also demonstrate an ability to assimilate short-chain fatty acids and synthesize poly-β-hydroxyalkano ... | 2010 | 20633046 |
| modelling the population dynamics and metabolic diversity of organisms relevant in anaerobic/anoxic/aerobic enhanced biological phosphorus removal processes. | in this study, enhanced biological phosphorus removal (ebpr) metabolic models are expanded in order to incorporate the competition between polyphosphate accumulating organisms (paos) and glycogen accumulating organisms (gaos) under sequential anaerobic/anoxic/aerobic conditions, which are representative of most full-scale ebpr plants. since paos and gaos display different denitrification tendencies, which is dependent on the phylogenetic identity of the organism, the model was separated into six ... | 2010 | 20580055 |
| candidatus monilibacter spp., common bulking filaments in activated sludge, are members of cluster iii defluviicoccus. | two alphaproteobacterial neisser negative 'nostocoida limicola' morphotypes differing slightly in their trichome diameter and filament regularity were dominant populations in the bendigo, victoria, australia activated sludge community removing phosphorus (p). neither responded to the fish probes available for any of the other alphaproteobacterial 'n. limicola' morphotypes. instead both fluoresced with the df988 fish probe designed originally to target alphaproteobacterial cluster ii defluviicocc ... | 2009 | 19679419 |
| induction of membrane permeability in escherichia coli mediated by lysis protein of the cole7 operon. | a glycogen nonpolyphosphate-accumulating organism (gao) enrichment culture dominated by the alphaproteobacteria cluster 1 defluviicoccus was investigated to determine the metabolic pathways involved in the anaerobic formation of polyhydroxyalkanoates, carbon storage polymers important for the proliferation of microorganisms in enhanced biological phosphorus removal processes. fish-microautoradiography and post-fish fluorescent chemical staining confirmed acetate assimilation as polyhydroxyalkano ... | 2009 | 19673051 |
| anaerobic central metabolic pathways active during polyhydroxyalkanoate production in uncultured cluster 1 defluviicoccus enriched in activated sludge communities. | a glycogen nonpolyphosphate-accumulating organism (gao) enrichment culture dominated by the alphaproteobacteria cluster 1 defluviicoccus was investigated to determine the metabolic pathways involved in the anaerobic formation of polyhydroxyalkanoates, carbon storage polymers important for the proliferation of microorganisms in enhanced biological phosphorus removal processes. fish-microautoradiography and post-fish fluorescent chemical staining confirmed acetate assimilation as polyhydroxyalkano ... | 2009 | 19622073 |
| community structure evolution and enrichment of glycogen-accumulating organisms producing polyhydroxyalkanoates from fermented molasses. | an open mixed culture was enriched with glycogen-accumulating organisms (gaos) by using a sequencing batch reactor and treating an agroindustrial waste (sugar cane molasses) under cyclic anaerobic-aerobic conditions. over a 1-year operating period, the culture exhibited a very stable gao phenotype with an average polyhydroxyalkanoate (pha) content of 17% total suspended solids. however, the gao microbial community evolved over the course of operation to a culture exhibiting unusual characteristi ... | 2009 | 19465533 |
| kinetic and metabolic aspects of defluviicoccus vanus-related organisms as competitors in ebpr systems. | a reactor was successfully enriched (90% as shown by fluorescence in situ hybridization) in defluviicoccus vanus-related organisms presenting a glycogen accumulating organisms (gao) phenotype. initial batch tests were performed using anaerobic/aerobic conditions to assess the capacity of different carbon sources utilization frequently abundant in wastewater: acetate, propionate, butyrate, valerate and glucose. acetate and propionate were totally consumed in the anaerobic phase as well as butyrat ... | 2008 | 19001727 |
| production of polyhydroxyalkanoates by glycogen accumulating organisms treating a paper mill wastewater. | a process for production of polyhydroxyalkanoates (pha) by activated sludge treating a paper mill wastewater was investigated. the applied strategy was to select for glycogen accumulating organisms (gaos) by alternating anaerobic/aerobic conditions. acidogenic fermentation was used as pretreatment to convert various organic compounds to volatile fatty acids which are preferable substrates for pha production. enrichment resulted in a culture dominated by gaos related to defluviicoccus vanus (56%) ... | 2008 | 18701781 |
| ecophysiology of polyphosphate-accumulating organisms and glycogen-accumulating organisms in a continuously aerated enhanced biological phosphorus removal process. | to investigate the ecophysiology of populations of polyphosphate-accumulating organisms (pao) and glycogen-accumulating organisms (gao) in communities of a novel acetate fed process removing phosphate from wastewater. attempts were made to see if acetate could be replaced by an alternative carbon source which did not support the growth of the gao. | 2008 | 18557960 |
| facs enrichment and identification of floc-associated alphaproteobacterial tetrad-forming organisms in an activated sludge community. | a precise phylogenetic identity of the defluviicoccus-related glycogen-accumulating organisms (gao) observed after fish probing in a novel activated sludge process removing phosphorus was sought with the aim of exploring the phylogenetic diversity of this important group. these organisms, whose sequences were not revealed in previously generated community wide 16s rrna gene clone libraries, were identified using flow cytometry cell sorting of fish-positive cells. sequencing of a 16s rrna gene cl ... | 2008 | 18557782 |
| characterisation of polyhydroxyalkanoate copolymers with controllable four-monomer composition. | polyhydroxyalkanoate (pha) copolymers comprising the four monomers 3-hydroxybutyrate (3hb), 3-hydroxyvalerate (3hv), 3-hydroxy-2-methylvalerate (3hmv) and 3-hydroxy-2-methylbutyrate (3hmb) were generated using the recently discovered defluviicoccus vanus-related glycogen accumulating organisms (dvgaos) under anaerobic conditions without applying any nutrient limitations. the composition could be manipulated in a defined range by modifying the ratio of propionate and acetate provided in the feed ... | 2008 | 18313162 |
| factors affecting the microbial populations at full-scale enhanced biological phosphorus removal (ebpr) wastewater treatment plants in the netherlands. | the influence of operating and environmental conditions on the microbial populations of the enhanced biological phosphorus removal (ebpr) process at seven full-scale municipal activated sludge wastewater treatment plants (wwtps) in the netherlands was studied. data from the selected wwtps concerning process configuration, operating and environmental conditions were compiled. the ebpr activity from each plant was determined by execution of anaerobic-anoxic-aerobic batch tests using fresh activate ... | 2008 | 18272198 |
| bioenergetic models for acetate and phosphate transport in bacteria important in enhanced biological phosphorus removal. | most of our understanding of the physiology of microorganisms is the result of investigations in pure culture. however, in order to understand complex environmental processes, there is a need to investigate mixed microbial communities. this is true for enhanced biological phosphorus removal (ebpr), an environmental process that results in the enrichment of the polyphosphate-accumulating organism accumulibacter spp. and the glycogen non-polyphosphate accumulating organism defluviicoccus spp. we i ... | 2008 | 18211269 |
| the denitrification capability of cluster 1 defluviicoccus vanus-related glycogen-accumulating organisms. | the denitrification capability of cluster 1 defluviicoccus vanus-related glycogen-accumulating organisms (dvgaos) is investigated. a sequencing batch reactor (sbr) fed with acetate as the sole carbon source was operated under alternating anaerobic-aerobic conditions to enrich cluster 1 dvgaos. fluorescence in situ hybridization (fish) showed that more than 85% of the bacterial population present in the reactor bound to the probes previously designed for cluster 1 dvgaos. a series of batch tests ... | 2008 | 18023040 |
| ecophysiology of defluviicoccus-related tetrad-forming organisms in an anaerobic-aerobic activated sludge process. | a group of uncultured tetrad-forming organisms (tfos) was enriched in an acetate-fed anaerobic-aerobic sequencing membrane bioreactor showing deteriorated enhanced biological phosphorus removal capacity. based on 16s rrna gene clone library and fluorescence in situ hybridization (fish) analyses, these tfos were identified as novel members of the defluviicoccus cluster in the alphaproteobacteria, accounting for 90 +/- 5% of the eubmix fish-detectable bacterial cell area in the reactor biomass. mi ... | 2007 | 17504486 |
| anaerobic metabolism of defluviicoccus vanus related glycogen accumulating organisms (gaos) with acetate and propionate as carbon sources. | the anaerobic uptake of acetate and propionate as single and dual carbon sources by the putative defluviicoccus vanus related glycogen accumulating organisms (dvgaos) is investigated. a high enrichment of dvgaos, representing 95+/-3% of the bacterial community bound to the eubmix probes, was achieved in a lab-scale reactor operated under alternating anaerobic and aerobic conditions with acetate as the sole carbon source. the culture is able to take up both acetate and propionate under anaerobic ... | 2007 | 17368713 |
| ecology of the microbial community removing phosphate from wastewater under continuously aerobic conditions in a sequencing batch reactor. | all activated sludge systems for removing phosphate microbiologically are configured so the biomass is cycled continuously through alternating anaerobic and aerobic zones. this paper describes a novel aerobic process capable of decreasing the amount of phosphate from 10 to 12 mg p liter(-1) to less than 0.1 mg p liter(-1) (when expressed as phosphorus) over an extended period from two wastewaters with low chemical oxygen demand. one wastewater was synthetic, and the other was a clarified effluen ... | 2007 | 17293509 |
| abundance and ecophysiology of defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes. | the activity of glycogen-accumulating organisms (gaos) in enhanced biological phosphorus removal (ebpr) wastewater treatment plants has been proposed as one cause of deterioration of ebpr. putative gaos from the alphaproteobacteria, defluviicoccus spp. (including d. vanus), were studied in full-scale ebpr plants to determine their distribution, abundance and ecophysiology. fluorescence in situ hybridization (fish) demonstrated that defluviicoccus spp. were generally low in abundance; however, in ... | 2007 | 17185546 |
| anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source. | in the microbial competition observed in enhanced biological phosphorus removal (ebpr) systems, an undesirable group of micro-organisms known as glycogen-accumulating organisms (gaos) compete for carbon in the anaerobic period with the desired polyphosphate-accumulating organisms (paos). some studies have suggested that a propionate carbon source provides paos with a competitive advantage over gaos in ebpr systems; however, the metabolism of gaos with this carbon source has not been previously i ... | 2006 | 16946271 |
| microbial succession of glycogen accumulating organisms in an anaerobic-aerobic membrane bioreactor with no phosphorus removal. | the succession of glycogen accumulating organisms (gaos) has been observed in an acetate-fed, anaerobic-aerobic sequencing membrane bioreactor (mbr) operated for 260 days without enhanced biological phosphorus removal (ebpr) activity. semi-quantitative fluorescence in situ hybridization results showed that a gammaproteobacterial lineage gb frequently observed in ebpr processes was initially the numerically dominant species (50-66% of total cells) of the gao in the mbr from day 1 to day 38. durin ... | 2006 | 16898134 |
| putative glycogen-accumulating organisms belonging to the alphaproteobacteria identified through rrna-based stable isotope probing. | deterioration of enhanced biological phosphorus removal (ebpr) has been linked to the proliferation of glycogen-accumulating organisms (gaos), but few organisms possessing the gao metabolic phenotype have been identified. an unidentified gao was highly enriched in a laboratory-scale bioreactor and attempts to identify this organism using conventional 16s rrna gene cloning had failed. therefore, rrna-based stable isotope probing followed by full-cycle rrna analysis was used to specifically identi ... | 2006 | 16436430 |
| defluvicoccus vanus gen. nov., sp. nov., a novel gram-negative coccus/coccobacillus in the 'alphaproteobacteria' from activated sludge. | a novel gram-negative coccus/coccobacillus, strain ben 114(t), growing in tetrads, clusters or aggregates, was isolated from activated sludge by micromanipulation. 16s rrna gene sequence analysis revealed that it belonged to the 'alphaproteobacteria', with no close relatives among cultured bacterial isolates. on the basis of phylogenetic data, this organism is considered to belong to a new genus, defluvicoccus, represented by the species defluvicoccus vanus sp. nov., a name chosen because of the ... | 2005 | 16166717 |
| In situ profiling of microbial communities in full-scale aerobic sequencing batch reactors treating winery waste in australia. | On-site aerobic sequencing batch reactor (SBR) treatment plants are implemented in many Australian wineries to treat the large volumes of associated wastewater they generate. Yet very little is known about their microbiology. This paper represents the first attempt to analyze the communities of three such systems sampled during both vintage and nonvintage operational periods using molecular methods. Alphaproteobacterial tetrad forming organisms (TFO) related to members of the genus Defluviicoccu ... | 2011 | 21875070 |