| zucchini green mottle mosaic virus is a new tobamovirus; comparison of its coat protein gene with that of kyuri green mottle mosaic virus. | a novel virus we call zucchini green mottle mosaic virus (zgmmv) was isolated from zucchini squash and its properties were determined. the size and shape of its virions, and other properties suggest that the virus is a tobamovirus. the coat protein (cp) genes of zgmmv and kyuri green mottle mosaic virus (kgmmv), which also infects zucchini squash plants, were cloned and their nucleotides sequences were determined. the cp genes of zgmmv and kgmmv are composed of 161 amino acid residues, and they ... | 2000 | 11205120 |
| plant virus cdna chip hybridization for detection and differentiation of four cucurbit-infecting tobamoviruses. | a plant virus cdna chip was developed by using viral cdna clones and microarray technology. the cdna chip was designed for detection and differentiation of the four species of selected cucurbit-infecting tobamoviruses [target viruses: cucumber green mottle mosaic virus (cgmmv); cucumber fruit mottle mosaic virus (cfmmv); kyuri green mottle mosaic virus (kgmmv); and zucchini green mottle mosaic virus (zgmmv)]. the chip consisted of cdna clones of the four cucurbit-infecting tobamoviruses, two tar ... | 2003 | 12757916 |
| genome structure and production of biologically active in vitro transcripts of cucurbit-infecting zucchini green mottle mosaic virus. | abstract the complete nucleotide sequence of the zucchini green mottle mosaic virus (zgmmv), a new member of the genus tobamovirus, has been determined. the genome of zgmmv is 6,513 nucleotides long and contains four open reading frames coding for proteins of 131, 189, 28, and 17 kda from the 5' to 3' end, respectively. the 5'- and 3'-non-translated regions consist of 59 and 163 residues, respectively. the sequences of the viral proteins exhibit high identity to the proteins of the members of th ... | 2002 | 18943088 |
| [complete genomic sequence of a watermelon isolate of cucumber green mottle mosaic virus in northern china]. | the complete genomic sequence of a watermelon isolate of cucumber green mottle mosaic virus (cgmmv-ln) in liaoning province was determined and compared with other cucurbit-infecting tobamoviruses. the genomic rna of cgmmv-ln comprised 6422 nt, and 5'- and 3'- noncoding regions consisted of 59 nt and 175 nt, respectively. the encoded four proteins were two replicase proteins of 186 kd and 129 kd, move protein of 29 kd and coat protein of 17.4 kd. the alignment results of complete nucleotide seque ... | 2009 | 19437890 |
| complete nucleotide sequences and construction of full-length infectious cdna clones of cucumber green mottle mosaic virus (cgmmv) in a versatile newly developed binary vector including both 35s and t7 promoters. | seed-transmitted viruses have caused significant damage to watermelon crops in korea in recent years, with cucumber green mottle mosaic virus (cgmmv) infection widespread as a result of infected seed lots. to determine the likely origin of cgmmv infection, we collected cgmmv isolates from watermelon and melon fields and generated full-length infectious cdna clones. the full-length cdnas were cloned into newly constructed binary vector pjy, which includes both the 35s and t7 promoters for versati ... | 2017 | 27913980 |
| simultaneous multiplex pcr detection of seven cucurbit-infecting viruses. | two multiplex polymerase chain reaction (pcr) systems using dual priming oligonucleotide (dpo) primers were developed for the simultaneous detection of seven cucurbit-infecting viruses. one system allows for the detection of papaya ringspot virus, watermelon mosaic virus, and zucchini yellow mosaic virus, whereas the other permits the detection of cucumber green mottle mosaic virus, cucumber fruit mottle mosaic virus, kyuri green mottle mosaic virus, and zucchini green mottle mosaic virus. viral ... | 2014 | 24937806 |