| procedures for obtaining sectional views of fungal fructifications by scanning electron microscopy. | procedures for sectioning fungal fructifications in host tissues or on artificial media are described, which allow observation of internal structures by scanning electron microscopy. perithecia of ceratocystis fimbriata and phyllachora graminis, and telia of puccinia xanthii showed excellent preservation of exposed structures in sections which were osmium-coated before being dried. while similar preservation was obtained in sectioned acervuli of lecanosticta acicola and marssonia juglandis and i ... | 1976 | 974917 |
| development, comparison, and validation of real-time and conventional pcr tools for the detection of the fungal pathogens causing brown spot and red band needle blights of pine. | dothistroma pini, d. septosporum, and lecanosticta acicola are fungal pathogens that cause severe foliage diseases in conifers. all three pathogens are listed as quarantine organisms in numerous countries throughout the world and, thus, are subject to specific monitoring. detection and identification of these pathogens still often relies on cumbersome and unsatisfactory methods that are based upon the morphological characterization of fungal fruiting bodies and conidia. in this study, we present ... | 2010 | 19968556 |
| dna barcoding of mycosphaerella species of quarantine importance to europe. | the eu 7th framework program provided funds for quarantine barcoding of life (qbol) to develop a quick, reliable and accurate dna barcode-based diagnostic tool for selected species on the european and mediterranean plant protection organization (eppo) a1/a2 quarantine lists. seven nuclear genomic loci were evaluated to determine those best suited for identifying species of mycosphaerella and/or its associated anamorphs. these genes included β-tubulin (btub), internal transcribed spacer regions o ... | 2012 | 23606768 |