| nucleoside triphosphate phosphohydrolase associated with cytoplasmic polyhedrosis virus. | nucleoside triphosphate phosphohydrolase ec 3.6.1.15 activity was found to be included in silkworm cytoplasmic polyhedrosis (cp) virus, which synthesizes mrna carrying the 5'-terminal modification. this enzyme releases orthophosphate from the gamma-position in a nucleoside triphosphate, leaving nucleoside diphosphate. the rate of hydrolysis of atp is faster than that of any other ribonucleoside triphosphate. deoxy atp is hydrolyzed rather faster than atp. however, polynucleotides carrying tripho ... | 1977 | 14944 |
| a blocked structure at the 5' terminus of mrna from cytoplasmic polyhedrosis virus. | | 1975 | 163011 |
| the process of formation of the 5' -terminal modified structure in messenger rna of cytoplasmic polyhedrosis virus. | | 1976 | 178543 |
| molecular weights of the rna genome segments of a cytoplasmic polyhedrosis virus determined by a new comparative approach. | | 1977 | 319594 |
| electron microscope observations on the development of cytoplasmic polyhedrosis virus in scotogramma trifolii rott. (lepidoptera, noctuidae). | | 1977 | 329953 |
| [in vivo and in vitro studies of a cytoplasmic polyhedrosis virus isolated from euxoa scandens, riley (noctuidae, agrotinae)(author's transl)]. | a virus isolated from the larvaes of euxoa scandens (noctuidae, agrotinae) was studied by histological and histochemical techniques, and by electron microscopic observation of virus suspensions and ultrathin sections of infected tissues. our studies demonstrated that the virus in icosahedral in shape, measures 70 nm and belongs to the cytoplasmic polyhedrosis virus group. only the midgut cells of the larvae were found to be infected and the virus particles were localized in inclusion bodies most ... | 1977 | 336008 |
| reproducible alteration of cytoplasmic polyhedrosis virus double-stranded rna genome patterns on laboratory passage. | | 1978 | 339515 |
| the multicomponent genome of a different cytoplasmic polyhedrosis virus isolated from heliothis armigera. | the genome of a new cytoplasmic polyhedrosis virus (cpv) isolated from heliothis armigera has been shown to consist of 10 double-stranded rna segments, as in other cpvs, but the genome profile on page bears no resemblance to previously described cpvs isolated from h.armigera. | 1978 | 361635 |
| a new host, simulium argyreatum meig., for the cytoplasmic polyhedrosis virus of blackflies in czechoslovakia. | the cytoplasmic polyhedrosis virus (cpv) from the midgut of blackfly larvae is recorded from a population of simulium argyreatum in czechoslovakia. a 7.7% infection of 2766 sampled larvae was manifested by white spots in their body. 85% of the white-spotted larvae were infected with cpv when sampled in june. in august 9% of 1377 larvae had white spots but only 10.6% of them were with cpv. out of 823 collected pupae, only 3 imperfect pupae had the virus in apparent form. other infections producin ... | 1978 | 369963 |
| the blocked and methylated 5' terminal sequence of a specific cellular messenger: the mrna for silk fibroin of bombyx mori. | the messenger rna for silf fibroin, laveled with 32po4 and methyl-3h l-methionine, was purified to near homogeneity from the posterior silk gland of the sildworm bombyx mori, and the sequence of a methylated, rnaase t2-resistant structure was determined. this sequence is similar structurally to 5' terminal blocked and methylated sequences found on the total populations of polyadenylated eucaryotic cellular and certain viral mrnas. the rnaase t2-resistant oligomer from fibroin mrna was cleaved by ... | 1976 | 947545 |
| a cytoplasmic polyhedrosis virus from the larval blackflies cnephia mutata and prosimulium mixtum (diptera:simuliidae). | | 1975 | 1090682 |
| iridovirus and cytoplasmic polyhedrosis virus in the freshwater daphnid simocephalus expinosus. | | 1975 | 1090849 |
| transmission, diagnosis, and control of cytoplasmic polyhedrosis virus in colonies of heliothis virescens. | | 1975 | 1097538 |
| isolation of a cytoplasmic polyhedrosis virus by physical and immunological techniques. | procedures are described for the separation and purification of cytoplasmic polyhedrosis viruses obtained from multiply infected heliothis armigera larvae. separation was achieved by differential centrifugation and density gradient zone electrophoresis followed by complexing with nuclear polyhedrosis virus specific antibody. the yield of cytoplasmic polyhedrosis virus was increased by passage in larvae reared on synthetic media. | 1975 | 1098037 |
| antibodies ot double-stranded rna: specificity and serum nucleases. | an antiserum was prepared in rabbits to the synthetic double-stranded ribonucleic acid (ds rna) poly ri:rc. using a liquid-phase radioimmunoassay, the antiserum cross-reacted with a natural ds rna isolated from the cytoplasmic polyhedrosis virus of the silkworm, binding 95% of the rna at a 1 : 20 serum dilution. preliminary tests of the specificity of the antiserum showed that it did not bind single-stranded rna (ss rna) or deoxyribonucleic acid (dna), but also revealed that the serum contained ... | 1975 | 1107429 |
| isolation of cdna clones coding for humoral lectin of silkworm, bombyx mori, larvae. | in the hemolymph of the silkworm, bombyx mori, lectin with hemagglutinating activity against sheep red blood cells increases at larval-larval ecdysis and at spinning stage (suzuki and natori, 1983) and is induced by infection with cytoplasmic polyhedrosis virus. a bombyx lectin polypeptide with molecular weight approx 280k is responsible for hemagglutinating activity, since antiserum raised against this polypeptide inhibited hemagglutinating activity. the site of synthesis of bombyx lectin was d ... | 1992 | 1541849 |
| entomopathogens of phlebotomine sand flies: laboratory experiments and natural infections. | the susceptibility of different geographical strains of phlebotomus papatasi to a cytoplasmic polyhedrosis virus (cpv) was determined experimentally by feeding polyhedra to larvae. of the indian p. papatasi, 15.6% became infected, whereas egyptian p. papatasi were mostly refractory. infection rates were not augmented in colony flies from the jordan valley, 23.8% of which were naturally infected with cpv. the infectivity of serratia marcescens and beauvaria bassiana to p. papatasi were determined ... | 1991 | 1783777 |
| x-ray powder pattern analysis of cytoplasmic polyhedrosis virus inclusion bodies. | cytoplasmic polyhedrosis virus is an insect reovirus which is occluded in crystalline inclusion bodies that form in the mid-gut of certain insects. inclusion bodies of cytoplasmic polyhedrosis virus from bombyx mori were isolated and purified. these crystalline bodies, about 1-3 microns in linear size, were compacted in a capillary tube while immersed in buffer. x-ray diffraction photographs showed powder rings, extending to 8.2 a resolution, which could be indexed with a cell measuring a = b = ... | 1991 | 1984644 |
| cytoplasmic polyhedrosis virus classification by electropherotype; validation by serological analyses and agarose gel electrophoresis. | serological analyses of several different cytoplasmic polyhedrosis viruses (cpvs), including two type 1 cpvs from bombyx mori, type 1 cpv from dendrolimus spectabilis, type 12 cpv from autographa gamma, type 2 cpv from inachis io, type 5 cpv from orgyia pseudotsugata and type 5 cpv from heliothis armigera, demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsrna electropherotypes. the dsrnas of these viruses w ... | 1989 | 2499658 |
| nucleotide sequence of the polyhedrin gene of euxoa scandens cytoplasmic polyhedrosis virus (escpv). | the double-stranded rna genome of euxoa scandens cytoplasmic polyhedrosis virus (escpv) was reversely transcribed to the double-stranded dna and cloned into pibi30. the complete nucleotide sequence of cloned genome segment 10, which encodes the virus polyhedrin polypeptide, was determined. the escpv polyhedrin gene consists of 881 bp and possesses an open reading frame that codes for a polypeptide of 269 amino acids (mw 30.1k), consistent with an apparent mw of 30k determined by sds-page for pur ... | 1989 | 2650462 |
| nucleotide sequence of the polyhedrin gene of bombyx mori cytoplasmic polyhedrosis virus a strain with nuclear localization of polyhedra. | bombyx mori cytoplasmic polyhedrosis virus (bmcpv) strain h produces many hexahedral polyhedra (inclusion bodies) in the cytoplasm of insect midgut epithelial cells. the mutant a strain, however, produces polyhedra in the nucleus. we determined cdna sequences of the polyhedrin genes, the smallest of the 10 genome segments, of these two strains. the polyhedrin genes of the h and a strains were 944 bp long, and encoded polypeptides of 248 amino acids (mr 28,500) and 252 amino acids (mr 29,000), re ... | 1989 | 2661724 |
| molecular cloning and characterization of cytoplasmic polyhedrosis virus polyhedrin and a viable deletion mutant gene. | the double-stranded rna genome of bombyx mori cytoplasmic polyhedrosis virus (cpv) was converted to double-stranded dna and cloned into plasmid pbr322. the complete nucleotide sequence of cloned genome segment 10, which encodes virus polyhedrin polypeptide, was determined. the cpv polyhedrin gene consists of 942 based pairs and possesses a long open reading frame that codes for a polypeptide of 248 amino acids (molecular weight, 28,500), consistent with an apparent molecular weight of 28,000 pre ... | 1988 | 3275433 |
| coupled transcription-translation of silkworm cytoplasmic polyhedrosis virus injected into oocytes of the frog, xenopus laevis. | the cytoplasmic polyhedrosis virus of the silkworm, bombyx mori, contains a ten-segmented, double-stranded rna genome and five species of polypeptides, v1 (mr 146,000), v2 (mr 140,000), v3 (mr 128,000)), v4 (mr 62,000), and v5 (mr 32,000). the virus contains an rna-dependent rna polymerase that transcribes the duplex genome rna to form mrna either in the infected animals or under appropriate conditions in vitro. we co-microinjected the virus, [alpha-32p]gtp and actinomycin d into oocytes of the ... | 1988 | 3283114 |
| the protein subunits of the double-stranded rna dependent rna polymerase and methyltransferase of the cytoplasmic polyhedrosis virus of silkworm, bombyx mori. | the particles of cpv of silkworm contain double-stranded rna polymerase and methyltransferase. it was reported in a previous paper that the genome-enzyme complex could be isolated. the genome-enzyme complex shows high enzyme activity of rna polymerase and methyltransferase in spite of the fact that it consists of only 5 percent of the protein. in order to clarify the protein subunits of the rna polymerase and methyltransferase, two methods were adopted. the sds-polyacrylamide gel electrophoretog ... | 1986 | 3299695 |
| a new method for extracting circular and supercoiled genome segments from cytoplasmic polyhedrosis virus. | the genome of a cytoplasmic polyhedrosis virus (cpv) consisting of 10 segments was extracted by a new two-step extraction method. the results from polyacrylamide gel electrophoresis, phenol and diepoxybutane treatment and electron microscopic examination indicated the extracted genome segments to have circular and supercoiled structures as genome-protein(s) complexes. multiple cycles of the transcription of each segmented genome of cpv may take place on the circular structure of the segment. | 1986 | 3539959 |
| a cytoplasmic polyhedrosis virus of triphaena pronuba (l.) (lepidoptera, noctuidae). | | 1970 | 4099856 |
| electron microscopic study on rna of cytoplasmic polyhedrosis virus of the silkworm. | | 1969 | 4308564 |
| enzyme activities associated with cytoplasmic polyhedrosis virus from bombyx mori. ii. comparative studies. | | 1973 | 4366479 |
| 5'-terminal nucleotide sequences of the double-stranded rna of silkworm cytoplasmic polyhedrosis virus. | | 1974 | 4369455 |
| a cytoplasmic polyhedrosis virus in midgut cells of anopheles stephensi and in the sporogonic stages of plasmodium berghei yoelii. | although it has been known for some time that nuclear and cytoplasmic polyhedrosis viruses may infect the larval stages of mosquitos capable of transmitting mammalian malaria this paper reports for the first time the presence of a cytoplasmic polyhedrosis virus in adult anopheles stephensi. the virus was shown to be present in sporogonic stages of plasmodium berghei yoelii with which the mosquitos were infected. it is possible that other viruses may affect both vector and malaria parasites. furt ... | 1972 | 4557906 |
| [rna polymerase associated with double-stranded rna virus, with special reference to rna polymerase associated with cytoplasmic polyhedrosis virus of the silkworm]. | | 1973 | 4567925 |
| transcription of double-stranded rna in cytoplasmic polyhedrosis virus in vtro. | | 1973 | 4574875 |
| single-stranded rna synthesis in vitro by the rna polymerase associated with cytoplasmic polyhedrosis virus containing double-stranded rna. | | 1973 | 4582169 |
| the synthesis of virus-specific single-stranded rna in larvae of bombyx mori infected with a cytoplasmic polyhedrosis virus. | | 1973 | 4589642 |
| replication of a cytoplasmic polyhedrosis virus in an established cell line of trichoplusia in cells. | | 1974 | 4598710 |
| 5'-terminal structure of messenger rna transcribed by rna polymerase of silkworm cytoplasmic polyhedrosis virus containing double-stranded rna. | | 1974 | 4605463 |
| the structural proteins and rna components of a cytoplasmic polyhedrosis virus from nymphalis io (lepidoptera: nymphalidae). | | 1974 | 4612108 |
| sequence homology studies between the double-stranded rna genomes of cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus strains 1, 2 and 3. | | 1974 | 4615076 |
| biochemical properties of polyhedra and virus particles of the cytoplasmic polyhedrosis virus of bombyx mori. | | 1974 | 4619114 |
| a radioimmunoassay for the cytoplasmic polyhedrosis virus from bombyx mori. | | 1974 | 4619115 |
| comparison of the structure and polypeptide composition of three double-stranded ribonucleic acid-containing viruses (diplornaviruses): cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus. | iodination of reovirus, cytoplasmic polyhedrosis virus (cpv), and wound tumor virus (wtv), and their respective subviral forms, followed by analysis of the labeled polypeptides by using polyacrylamide gel electrophoresis, has been used to compare the protein contents of these three diplornaviruses. this approach, when combined with electron microscopy and buoyant density determinations, appears capable of localizing individual polypeptides in some of the viral and subviral forms. cpv (p = 1.435 ... | 1972 | 4636394 |
| comparison of the 3' termini of discrete segments of the double-stranded ribonucleic acid genomes of cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus. | the 3' terminal nucleosides of the isolated components of double-stranded ribonucleic acids of reovirus, wound tumor virus, and cytoplasmic polyhedrosis virus were determined by labeling with tritiated sodium borohydride. all wound tumor virus and cytoplasmic polyhedrosis virus components appear to contain approximately equal amounts of u(oh) and c(oh) termini. reovirus segments have essentially only c(oh) termini. | 1972 | 4636396 |
| surface topography and shapes of polyhedral inclusion bodies of the cytoplasmic polyhedrosis virus. | | 1973 | 4696220 |
| identity of the 3'-terminal sequences in ten genome segments of silkworm cytoplasmic polyhedrosis virus. | | 1973 | 4742779 |
| [studies on the cytoplasmic polyhedrosis virus of silkworms]. | | 1968 | 4881543 |
| cytoplasmic polyhedrosis virus (cpv) of malacosoma disstria: rna polymerase activity in purified free viroin. | | 1972 | 5018698 |
| the 3'-termini of the genome rna segments of silkworm cytoplasmic polyhedrosis virus. | | 1972 | 5022191 |
| amino acid composition of proteins extracted from virions of cytoplasmic-polyhedrosis virus. | | 1971 | 5092822 |
| synthesis of ribonucleic acid resistant to actinomycin d in silkworm midguts infected with the cytoplasmic-polyhedrosis virus. | | 1971 | 5092827 |
| presence of cytoplasmic polyhedrosis virus in the hemolymph of heliothis virescens larvae and adults. | | 1971 | 5106447 |
| hemagglutination with cytoplasmic-polyhedrosis virus of the silkworm, bombyx mori. | | 1969 | 5344296 |
| nuclear-polyhedrosis and cytoplasmic-polyhedrosis virus infections in louisiana mosquitoes. | | 1969 | 5385139 |
| the site of rna synthesis of a cytoplasmic-polyhedrosis virus (cpv) in malacosoma disstria. | | 1970 | 5449739 |
| the isolation of cytoplasmic polyhedrosis virus from the white-marked tussock moth, orgyia leucostigma (smith). | | 1970 | 5484060 |
| rna in midgut of tussock moth, orgyia leucostigma, infected with cytoplasmic-polyhedrosis virus. | | 1970 | 5491276 |
| occluded and free virions in midgut cells of malacosoma disstria infected with cytoplasmic-polyhedrosis virus (cpv). | | 1970 | 5501205 |
| properties of rna from cytoplasmic-polyhedrosis virus of the white-marked tussock moth, orgyia leucostigma. | | 1970 | 5501206 |
| cytoplasmic polyhedrosis virus: rna synthesized in vivo and in vitro in infected midgut. | | 1971 | 5545513 |
| resistance of the alfalfa caterpillar, colias eurytheme, at high temperatures to a cytoplasmic-polyhedrosis virus and thermal inactivation point of the virus. | | 1968 | 5649247 |
| purification of a cytoplasmic-polyhedrosis virus of the silkworm, bombyx mori. | | 1969 | 5777835 |
| ineffectiveness of a microsporidian and a granulosis virus in activating a cytoplasmic polyhedrosis virus in the alfalfa caterpillar, colias eurytheme. | | 1969 | 5777841 |
| electron microscopic study of rna of cytoplasmic polyhedrosis virus of the silk worm. | | 1969 | 5812159 |
| resistance to peroral infection by the cytoplasmic-polyhedrosis virus in the silkworm, bombyx mori (linnaeus). | | 1965 | 5827559 |
| free amino acids in the hemolymph and midgut epithelium of the silkworm, bombyx mori (linnaeus), infected with cytoplasmic-polyhedrosis virus. | | 1965 | 5890108 |
| a cytoplasmic-polyhedrosis virus, smithiavirus pectinophorae sp. n. of the pink bollworm, pectinophora gossypiella (saunders). | | 1966 | 5905538 |
| the development of a cytoplasmic polyhedrosis virus in the spruce budworm, choristoneura fumiferana (clemens). | | 1966 | 5929158 |
| studies of two strains of cytoplasmic-polyhedrosis virus. | | 1966 | 5939382 |
| ribonucleic acid extracted from the cytoplasmic-polyhedrosis virus of the silkwom, bombyx mori. | | 1967 | 6073676 |
| the process of virus assembly in insect virus mixed infections. | interference occurred upon infection of the cabbage moth caterpillars (mamestra brassicae l.) with a mixture of nuclear polyhedrosis virus (npv) and cytoplasmic polyhedrosis virus (cpv), resulting in the impairment of virus assembly, and formation of abnormal nucleocapsids. at the same time protein supercapsids were produced normally, but contained no infectious virions. when insects were infected with related viruses, the virions developed as usual, but the protein supercapsids revealed abnorma ... | 1983 | 6139945 |
| a mode of action of rnase ci toward the various rnas. | a mode of action of rnase ci toward the various native rnas was examined. the mixture of trna from thermus thermophilus hb8 were exhaustively cleaved by rnase ci to produce 90% of acid-soluble fraction. among the minor nucleotides s2t were recognized by this enzyme. rnase ci generates a linear viroid molecule by introducing one single nick into viroid circular molecule. double stranded rna from rna virus, such as cytoplasmic polyhedrosis virus (cpv) from silk worm, was cleaved at some specific s ... | 1982 | 6191284 |
| midgut and viral associated proteases of heliothis armigera. | the role played by the gut juice of insects in the infective process of insect viruses was examined. analysis of larval gut extract of heliothis armigera by sds-page revealed protease activity associated with components of molecular weights 48,000 and 94,000. proteases were found to be associated with occlusion bodies and virions of both nuclear polyhedrosis virus (npv) and cytoplasmic polyhedrosis virus (cpv) infecting h. armigera. cpv occlusion bodies were dissolved by gut juice extract at ph ... | 1983 | 6337972 |
| characterization of a cytoplasmic polyhedrosis virus infecting manduca sexta. | a cytoplasmic polyhedrosis virus (cpv) infecting manduca sexta was characterized. the genome consisted of ten equimolar rna segments ranging in estimated molecular mass from 0.7 to 3.84 mdaltons and with a total estimated molecular mass of 21.75 mdaltons . the sizes of the individual rna segments provisionally placed this cpv within the type 8 classification of payne and rivers. the polyhedrin protein had an estimated molecular mass of 28 kdaltons . six virion-associated proteins were observed, ... | 1984 | 6373659 |
| some physical characteristics of a cytoplasmic polyhedrosis virus of heliothis armigera. | a number of physical properties of a cytoplasmic polyhedrosis virus of heliothis armigera were determined. these included a buoyant density of 1.44 g/cm3, a diffusion coefficient of 0.73 x 10(-7) cm2/sec, a partial specific volume of 0.688 ml/g, and a sedimentation coefficient of 399s. three different methods were used to determine an average molecular weight of 47.4 x 10(6). | 1980 | 6987192 |
| titration of a cytoplasmic polyhedrosis virus by a tissue microculture assay: some applications. | a simple tissue microculture technique was developed for the titration of a cytoplasmic polyhedrosis virus (cpv) from euxoa scandens. the procedure was similar to the 50% tissue culture infectious dose assay, but a single infected cell, detected by the presence of cytoplasmic polyhedra, was scored rather than the degeneration of cell monolayers. the filtration of cpv suspensions resulted in decreased virus titers under certain conditions. this microculture assay was used to determine the effect ... | 1980 | 6987194 |
| a unique class of compound, guanosine-nucleoside tetraphosphate g(5')pppp(5')n, synthesized during the in vitro transcription of cytoplasmic polyhedrosis virus of bombyx mori. structural determination and mechanism of formation. | two structurally different classes of oligonucleotides accumulate in vitro in cytoplasmic polyhedrosis virus (cpv) transcription mixtures in molar excess as compared to the completed rna products. the first class consists of oligonucleotides which correspond to the 5'-terminal sequence of the virus mrnas (referred to as initiator oligonucleotides). the major species of initiator oligonucleotides are (p)ppapg and (p)ppapgpn together with smaller amounts of homologous capped structures (furuichi, ... | 1982 | 7031064 |
| electrophoretic characterization of proteins and rna of cytoplasmic polyhedrosis virus (cpv) from euxoa scandens. | a cytoplasmic polyhedrosis virus (cpv) was isolated from euxoa scandens and propagated in vitro in lymantria dispar cells. the virions and polyhedra were analyzed by polyacrylamide gel electrophoresis. polyhedra produced in vivo and in vitro contained six polypeptides with identical relative mobilities while nonoccluded viral particles produced in vivo were composed of four polypeptides. the rna content of the purified virions extracted from infected midgut was resolved in ten segments with mole ... | 1981 | 7036955 |
| [3h-methyl]-methionine as possible methyl donor for formation of 5'-terminus of in vitro synthesized mrna of cytoplasmic polyhedrosis virus of silkworm bombyx mori. | the mrna of the cytoplasmic polyhedrosis virus of silkworm bombyx mori could be synthesized in vitro through the action of the virion associated rna-polymerase in the presence of [3h-methyl]-methionine instead of s-adenosyl-l-methionine. the 3h-mrna is isolated from the reaction mixture through a column of deae-sephadex a-25 and digested with nuclease p1 and snake venom phosphodiesterase. the results of paper electrophoresis show that the methyl group of methionine is incorporated into the 5'-te ... | 1981 | 7038870 |
| metabolic changes in diseased insects. ii. radioautographic studies on dna and rna synthesis in nuclear-polyhedrosis and cytoplasmic-polyhedrosis virus infections. | | 1968 | 5725007 |
| ultrastructure of a cytoplasmic polyhedrosis virus affecting the monarch butterfly, danaus plexippus. i. development of virus and normal polyhedra in the larva. | | 1968 | 5709882 |
| isolation of genome-enzyme complex from cytoplasmic polyhedrosis virus of silkworm bombyx mori. | the genome-enzyme complex is isolated from the silkworm cytoplasmic polyhedrosis virus with deae-sephadex column chromatography after ultraviolet irradiation. the genome-enzyme complex shows both rna-polymerase and methyltransferase activities while using 3h-utp and [3h-methyl]-s-adenosyle-l-methionine as substrates. like the double-stranded rna genome of cpv, the genome-enzyme complex could be separated into nine segments on polyacrylamide gel electrophoresis. it is demonstrated that the rna po ... | 1982 | 7048527 |
| the 5'-terminal nucleotide sequences of the double-stranded rna of human reovirus. | the 5'-terminal nucleotide sequences of human reovirus double-stranded rna were determined after labeling the rna with [(32)p]phosphate by polynucleotide kinase. the 5' terminal were labeled to only a limited extent prior to sequential oxidation, beta-elimination, and phosphomonoesterase treatment, indicating that the terminal phosphates were in a modified, blocked configuration. each genome segment, after removing the blocking group, contained the same two 5'-terminal sequences: gpapup in one c ... | 1974 | 4530278 |
| a cytoplasmic polyhedrosis virus in the phlebotomine sandfly lutzomyia longipalpis. | | 1995 | 7787233 |
| analysis of the c-polyhedrin genes from different geographical isolates of a type 5 cytoplasmic polyhedrosis virus. | the c-polyhedrin genes of two different geographic isolates of a type 5 cytoplasmic polyhedrosis virus (cpv) were cloned. a cpv infecting orgyia pseudotsugata (opcpv), isolated in the pacific northwest of the u.s.a., and a cpv infecting heliothis armigera, isolated in south africa, were studied. both genes were found to be 883 nucleotides in length and encoded a predicted protein of 246 residues (m(r) of 28,890). comparison of the nucleotide sequences of these two viruses with another type 5 geo ... | 1994 | 8046399 |
| expression in escherichia coli of the cloned polyhedrin gene of bombyx mori cytoplasmic polyhedrosis virus. | cloned cdna of genomic segment 10 of bombyx mori cytoplasmic polyhedrosis virus (cpv) was placed downstream from the lambda pl promoter in expression plasmid prc23 and expressed in escherichia coli cells. a polypeptide of the same molecular weight (28 kda) as natural polyhedrin was synthesized at the level of approximately 10% of total host cell protein. this polypeptide was identified as cpv polyhedrin (r-polyhedrin) after comparative studies. the r-polyhedrin did not form any crystalline struc ... | 1993 | 8286955 |
| isolation of a cytoplasmic polyhedrosis virus by physical and immunological techniques. 1975. | | 1993 | 8367399 |
| expression of bombyx mori cytoplasmic polyhedrosis virus polyhedrin in insect cells by using a baculovirus expression vector, and its assembly into polyhedra. | a cdna encoding the cytoplasmic polyhedrin of bombyx mori cytoplasmic polyhedrosis virus (bmcpv) strain h was introduced into an improved baculovirus expression vector which can be utilized to express foreign genes in the spodoptera frugiperda cell line iplb-sf-21ae (sf21 cells) and the b. mori cell line bmn. a recombinant virus produced large, cubic inclusion body-like structures in infected sf21 and bmn cells. western blot analysis showed that these structures were bmcpv polyhedra. this result ... | 1993 | 8423453 |
| effect of mutations on the intracellular localization of bombyx mori cytoplasmic polyhedrosis virus polyhedrin. | we have already cloned the polyhedrin genes of the wild-type strain h bombyx mori cytoplasmic polyhedrosis virus (bmcpv) and its mutant, strain a. in this work, polyhedrin genes of mutant bmcpv strains c1 and c2 were cloned and their nucleotide sequences were determined. the polyhedrin amino acid sequences of strains c1 and c2 were compared with that of strain h. strains c1 and c2 contained two and three sites of mutation in their polyhedrin genes, respectively. four amino acids (249rllv) were a ... | 1996 | 8558123 |
| characterization of the genome of cytoplasmic polyhedrosis virus. | it is possible to oxidize in situ the 3'-terminals of the double-stranded ribonucleic acid (rna) contained in structurally intact cytoplasmic polyhedrosis virus. the number of 3'-terminals subsequently found by reduction with tritiated sodium borohydride was equivalent to the number observed in rna first isolated from the virus and then oxidized and reduced. the viral rna appears to occur in 10 distinct polynucleotide segments present in equimolar amounts. | 1971 | 4329560 |
| sequence analysis and expression of the polyhedrin gene of choristoneura fumiferana cytoplasmic polyhedrosis virus (cfcpv). | the segmented double-stranded rna genome of choristoneura fumiferana cytoplasmic polyhedrosis virus (cfcpv) was extracted, polyadenylated, reverse-transcribed into cdna and cloned. the cdna clones that hybridized to the smallest genomic segment (segment 10) were identified, and its nucleotide sequence was determined. genome segment 10 of cfcpv was found to be 1171 nucleotides in length with a single open reading frame in one strand capable of coding a predicted protein of 258 residues (mr of 29, ... | 1997 | 9370308 |
| characterizations of natural and induced polyhedrin gene mutants of bombyx mori cytoplasmic polyhedrosis viruses. | bombyx mori cytoplasmic polyhedrosis virus (bmcpv) polyhedrin gene from natural mutant strain b, b2 and p was cloned and the amino acid sequence was compared with that of wild-type virus (strain h). chimeric polyhedrin genes (8 types) containing only one site of mutation within the amino acid sequence were also constructed. fifteen types of polyhedrin genes were introduced into a baculovirus expression vector and hexahedral, acicular, pyramidal, or amorphous polyhedra were formed in infected cel ... | 1998 | 9541610 |
| determination of the nucleotide sequence of bombyx mori cytoplasmic polyhedrosis virus segment 9 and its expression in bmn4 cells. | cloning and sequencing of segment 9 of bombyx mori cytoplasmic polyhedrosis virus (bmcpv) strains h and i were performed. the segment consisted of 1,186 bp harboring 5' and 3' noncoding regions and an open reading frame from positions 75 to 1037, encoding a protein with 320 amino acids, termed ns5. comparison of the nucleotide sequences of ns5 for the two strains indicated 37 point differences resulting in only six amino acid replacements. homology search showed that ns5 has localized similariti ... | 1998 | 9621035 |
| nucleotide sequence of bombyx mori cytoplasmic polyhedrosis virus segment 8. | the segments 8 (s8) of the 10 double-stranded rna genomes from bombyx mori cytoplasmic polyhedrosis virus (bmcpv) strains i and h were converted into cdnas, amplified by pcr, and cloned. the nucleotide sequence analysis of the two full-length s8 cdnas showed that the segments consist of 1328 nucleotides encoding putative proteins (p44) of 390 amino acids with molecular masses of about 44 kda, which have glutamic acid-rich and proline-rich domains in their central regions. they had quite high ide ... | 1998 | 9657006 |
| [cytoplasmic polyhedrosis virus and polyhedrin]. | | 1998 | 9752770 |
| gene assignments of a cytoplasmic polyhedrosis virus (type 2) from nymphalis io. | the 10 double stranded (ds) rnas from cytoplasmic polyhedrosis virus (type 2) from nymphalis io have been separated by polyacrylamide gel electrophoresis and fractionated into separate genes. separate and total dsrna were denatured using methyl mercury, and translated in a rabbit reticulocyte lysate. translation of unfractionated rna generated proteins that comigrated with virion and polyhedral structural proteins and six major non-structural proteins. translation of individual rnas, demonstrate ... | 1985 | 3892233 |
| two concentric protein shell structure with spikes of silkworm bombyx mori cytoplasmic polyhedrosis virus revealed by small-angle neutron scattering using the contrast variation method. | the overall and internal structures of the silkworm bombyx mori cytoplasmic polyhedrosis virus was investigated by small-angle neutron scattering using the contrast variation method. data were collected in aqueous buffer solutions containing 0, 50, 75, and 100% d2o in the q range of 0.002 to 0.0774 a-1 at 5 degrees c. the radius of gyration at infinite contrast was estimated to be 336 a. the contrast matching point of the virus was determined to correspond to about 50% d2o level, evidence that t ... | 1999 | 10220584 |
| electrophoretic separation of the plus and minus strands of rotavirus sa11 double-stranded rnas. | the genome of the rotaviruses consists of eleven segments of completely double-stranded rna (dsrna). to provide a method for separating and identifying the complementary plus and minus strand rnas within these segments, we have characterized their migration patterns under denaturing conditions on agarose-urea gels. virion-derived 3h-labelled dsrnas were resolved by electrophoresis on a polyacrylamide gel and the individual genome segments recovered by electroelution. upon electrophoresis in a lo ... | 1986 | 3016018 |
| "pretranscriptional capping" in the biosynthesis of cytoplasmic polyhedrosis virus mrna. | the in vitro synthesis of cytoplasmic polyhedrosis virus (cpv) mrna was previously shown to be dependent upon the presence of the methyl donor s-adenosylmethionine (adomet). we now find that the competitive inhibitor of methylation, s-adenosylhomocysteine (adohcy), also stimulates cpv mrna synthesis efficiently, resulting in the synthesis of viral mrnas containing 5'-terminal gpppa and ppa, rather than m(7)gpppam as observed with adomet. in addition to adohcy, other adomet analogues, including s ... | 1978 | 349555 |
| nucleotide sequences of genome segments 6 and 7 of bombyx mori cypovirus 1, encoding the viral structural proteins v4 and v5, respectively. | nucleotide sequence analyses of cdnas derived from the double-stranded rna genome segments 6 and 7 (s6 and s7) of bombyx mori cypovirus 1 (bmcpv-1) have revealed that they consist of 1796 and 1501 nucleotides encoding putative proteins of 561 and 448 amino acids with molecular masses of 63604 and 49875 (p64 and p50), respectively. the amino acid sequence of p64, which has a high leucine residue content (10%), contains a leucine zipper motif. antiserum raised against p64 specifically bound to a v ... | 2000 | 10725444 |
| "methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded rna. | s-adenosyl-l-methionine (sam) activated the virus-associated rna polymerase of cytoplasmic polyhedrosis virus in vitro. synthesis of single-stranded viral rna (mrna) proceeded depending on the presence of sam.a methyl residue of sam was incorporated into an rna molecule. a ribose moiety of adenylic acid in the 5'-terminal region of the nascent rna was methylated in the very early stage of the transcription. the dependence of the viral transcription on the presence of sam and the methylation of t ... | 1974 | 10793759 |
| molecular characterization of bombyx mori cytoplasmic polyhedrosis virus genome segment 4. | the complete nucleotide sequence of the genome segment 4 (s4) of bombyx mori cytoplasmic polyhedrosis virus (bmcpv) was determined. the 3,259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kda. western blot analysis showed that s4 encodes bmcpv protein vp3, which is one of the outer components of the bmcpv virion. sequence analysis of the deduced amino acid sequence of ... | 2001 | 11134312 |