| identification of proteins induced by polycyclic aromatic hydrocarbon in mycobacterium vanbaalenii pyr-1 using two-dimensional polyacrylamide gel electrophoresis and de novo sequencing methods. | protein profiles of mycobacterium vanbaalenii pyr-1 grown in the presence of high-molecular-weight polycyclic aromatic hydrocarbons (hmw pahs) were examined by two-dimensional gel electrophoresis (2-de). cultures of m. vanbaalenii pyr-1 were incubated with pyrene, pyrene-4,5-quinone (pq), phenanthrene, anthracene, and fluoranthene. soluble cellular protein fractions were analyzed and compared, using immobilized ph gradient (ipg) strips. more than 1000 gel-separated proteins were detected using a ... | 2004 | 15540208 |
| complete and integrated pyrene degradation pathway in mycobacterium vanbaalenii pyr-1 based on systems biology. | mycobacterium vanbaalenii pyr-1 was the first bacterium isolated by virtue of its ability to metabolize the high-molecular-weight polycyclic aromatic hydrocarbon (pah) pyrene. we used metabolic, genomic, and proteomic approaches in this investigation to construct a complete and integrated pyrene degradation pathway for m. vanbaalenii pyr-1. genome sequence analyses identified genes involved in the pyrene degradation pathway that we have proposed for this bacterium. to identify proteins involved ... | 2007 | 17085566 |
| diversity of soil mycobacterium isolates from three sites that degrade polycyclic aromatic hydrocarbons. | this paper investigates the diversity of polycyclic aromatic hydrocarbon (pah)-degrading mycobacterium isolates from three different sites within united states: montana, texas and indiana. | 2007 | 17578427 |
| substrate specificity and structural characteristics of the novel rieske nonheme iron aromatic ring-hydroxylating oxygenases nidab and nida3b3 from mycobacterium vanbaalenii pyr-1. | the rieske nonheme iron aromatic ring-hydroxylating oxygenases (rhos) nidab and nida3b3 from mycobacterium vanbaalenii pyr-1 have been implicated in the initial oxidation of high-molecular-weight (hmw) polycyclic aromatic hydrocarbons (pahs), forming cis-dihydrodiols. to clarify how these two rhos are functionally different with respect to the degradation of hmw pahs, we investigated their substrate specificities to 13 representative aromatic substrates (toluene, m-xylene, phthalate, biphenyl, n ... | 2010 | 20714442 |
| identification of novel potential antibiotics for tuberculosis by in silico structure-based drug screening. | the enoyl-acyl carrier protein reductase of mycobacterium tuberculosis (mtb) is a key enzyme of the type ii fatty acid synthesis system. it is involved in the production of mycolic acid and is a known target for isoniazid, an effective antibiotic for tuberculosis treatment. the increasing prevalence of tuberculosis in many areas of the world, which is associated with the rise of drug-resistant mtb strains, presents a major global health threat. in this study, we attempted to identify novel antib ... | 2011 | 21397998 |
| two distinct gene clusters encode pyrene degradation in mycobacterium sp. strain s65. | abstract a pyrene degrading bacterium, identified as mycobacterium sp. strain s65, was isolated from a jet-fuel contaminated site in quebec, canada. strain s65 utilized pyrene, phenanthrene, and fluoranthene as sole carbon and energy sources, but did not mineralize naphthalene, anthracene, or fluorene. pyrene mineralization was enhanced by adding benz[a]anthracene, benzo[a]pyrene, or phenanthrene as co-substrates. southern hybridization using the naphthalene inducible pyrene dioxygenase gene (ni ... | 2004 | 19712404 |
| potential of mycobacterium vanbaalenii as a model organism to study drug transporters of mycobacterium tuberculosis, mycobacterium marinum and mycobacterium ulcerans: homology analysis of m. tuberculosis drug transporters among mycobacterial species. | drug efflux pumps have been one of the important mechanisms of drug resistance in mycobacterium tuberculosis. there is a prerequisite to study the behavior and mechanisms of these drug efflux pumps in detail for being considered in future anti-tb drug designing. the need of a rapid grower non-pathogenic mycobacterium with significant genomic homology for such type of studies is often being felt. during microarray and real-time pcr analysis of drug efflux pump genes of m. tuberculosis, we found 1 ... | 2011 | 22127144 |
| mycobacterium tuberculosis rv2419c, the missing glucosyl-3-phosphoglycerate phosphatase for the second step in methylglucose lipopolysaccharide biosynthesis. | mycobacteria synthesize intracellular methylglucose lipopolysaccharides (mglp) proposed to regulate fatty acid synthesis. although their structures have been elucidated, the identity of most biosynthetic genes remains unknown. the first step in mglp biosynthesis is catalyzed by a glucosyl-3-phosphoglycerate synthase (gpgs, rv1208 in mycobacterium tuberculosis h37rv). however, a typical glucosyl-3-phosphoglycerate phosphatase (gpgp, ec3.1.3.70) for dephosphorylation of glucosyl-3-phosphoglycerate ... | 2011 | 22355692 |
| effect of activated carbon on microbial bioavailability of phenanthrene in soils. | bioavailability is a governing factor that controls the rate of biological degradation of hydrophobic organic contaminants in soil. among the solid phases that can adsorb hydrophobic organic contaminants in soil, black carbon (bc) exerts a particularly significant effect on phase distribution. however, knowledge on the effect of bc on the microbial availability of polycyclic aromatic hydrocarbons in soil is still limited. in the present study, the effect of a coal-derived activated carbon on the ... | 2009 | 19572767 |
| biotransformation of acridine by mycobacterium vanbaalenii. | cultures of mycobacterium vanbaalenii strain pyr-1 in a liquid medium were exposed to the toxic environmental contaminant acridine (260 microm). after incubation for 7 d, the cultures were extracted with ethyl acetate. metabolites were purified using high-performance liquid chromatography and analyzed by mass spectrometry and 1h nuclear magnetic resonance spectroscopy. four metabolites, 9,10-dihydroacridine, 4-hydroxyacridine, acridine cis-1 ,2-dihydrodiol, and acridin-9(10h)-one, were identifie ... | 2009 | 18717621 |
| the draft genome of mycobacterium aurum, a potential model organism for investigating drugs against mycobacterium tuberculosis and mycobacterium leprae. | mycobacterium aurum (m. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. the m. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02mb containing 5684 annotated protein-coding genes. a phylogenetic analysis using whole genome alignments positioned m. aurum close to mycobacterium vaccae and mycobacterium vanbaalenii, within a clade related to fast-gr ... | 2015 | 27649868 |
| genomic analysis of polycyclic aromatic hydrocarbon degradation in mycobacterium vanbaalenii pyr-1. | mycobacterium vanbaalenii pyr-1 is well known for its ability to degrade a wide range of high-molecular-weight (hmw) polycyclic aromatic hydrocarbons (pahs). the genome of this bacterium has recently been sequenced, allowing us to gain insights into the molecular basis for the degradation of pahs. the 6.5 mb genome of pyr-1 contains 194 chromosomally encoded genes likely associated with degradation of aromatic compounds. the most distinctive feature of the genome is the presence of a 150 kb majo ... | 2008 | 18421421 |
| isolation and identification of mycobacteria from soils at an illegal dumping site and landfills in japan. | in order to study the diversity and community of genus mycobacterium in polluted soils, we tried to isolate mycobacteria from 11 soil samples collected from an illegal dumping site and 3 landfills in japan. using culture methods with or without acanthamoeba culbertsoni, a total of 19 isolates of mycobacteria were obtained from 5 soil samples and 3 of them were isolated only by the co-culture method with the amoeba. conventional biochemical tests and sequencing of the hsp65, rpob, and 16s rrna ge ... | 2006 | 16858142 |
| molecular cloning and expression of genes encoding a novel dioxygenase involved in low- and high-molecular-weight polycyclic aromatic hydrocarbon degradation in mycobacterium vanbaalenii pyr-1. | mycobacterium vanbaalenii pyr-1 is able to metabolize a wide range of low- and high-molecular-weight (hmw) polycyclic aromatic hydrocarbons (pahs). a 20-kda protein was upregulated in pah-metabolizing m. vanbaalenii pyr-1 cells compared to control cultures. the differentially expressed protein was identified as a beta subunit of the terminal dioxygenase using mass spectrometry. pcr with degenerate primers designed based on de novo sequenced peptides and a series of plaque hybridizations were don ... | 2006 | 16461648 |
| molecular characterization of cytochrome p450 genes in the polycyclic aromatic hydrocarbon degrading mycobacterium vanbaalenii pyr-1. | mycobacterium vanbaalenii pyr-1 has the ability to degrade low- and high-molecular-weight polycyclic aromatic hydrocarbons (pahs). in addition to dioxygenases, cytochrome p450 monooxygenases have been implicated in pah degradation. three cytochrome p450 genes, cyp151 (pipa), cyp150, and cyp51, were detected and amplified by polymerase chain reaction from m. vanbaalenii pyr-1. the complete sequence of these genes was determined. the translated putative proteins were > or = 80% identical to other ... | 2006 | 16317545 |
| metabolic engineering of arabidopsis for remediation of different polycyclic aromatic hydrocarbons using a hybrid bacterial dioxygenase complex. | the widespread presence of polycyclic aromatic hydrocarbons (pahs) and their potential harm to various organisms has generated interest in efficiently eliminating these compounds from the environment. phytoremediation is an efficient technology for cleaning up pollutants. however, unlike microorganisms, plants lack the catabolic pathway for complete degradation of these dangerous groups of compounds. one way to enhance the potential of plants for remediation of these compounds is by transferring ... | 2014 | 25305469 |
| degradation of benz[a]anthracene by mycobacterium vanbaalenii strain pyr-1. | cultures of mycobacterium vanbaalenii strain pyr-1 grown in mineral salts medium and nutrients in the presence of benz[a]anthracene metabolized 15% of the added benz[a]anthracene after 12 days of incubation. neutral and acidic ethyl acetate extractable metabolites were isolated and characterized by high performance liquid chromatography (hplc) and uv-visible absorption, gas chromatography/mass (gc/ms) and nuclear magnetic resonance (nmr) spectral analysis. trimethylsilylation of the metabolites ... | 2005 | 15865344 |
| effects of ph on the degradation of phenanthrene and pyrene by mycobacterium vanbaalenii pyr-1. | the effects of ph on the growth of mycobacterium vanbaalenii pyr-1 and its degradation of phenanthrene and pyrene were compared at ph 6.5 and ph 7.5. various degradation pathways were proposed in this study, based on the identification of metabolites from mass and nmr spectral analyses. in tryptic soy broth, m. vanbaalenii pyr-1 grew more rapidly at ph 7.5 (mu'=0.058 h(-1)) than at ph 6.5 (mu'=0.028 h(-1)). however, resting cells suspended in phosphate buffers with the same ph values displayed a ... | 2004 | 15592827 |
| evidence for the existence of pah-quinone reductase and catechol-o-methyltransferase in mycobacterium vanbaalenii pyr-1. | polycyclic aromatic hydrocarbon (pah) quinone reductase (pqr) and catechol-o-methyltransferase (comt), from the pah-degrading mycobacterium vanbaalenii pyr-1, were demonstrated to be constitutive enzymes located in the soluble fraction of cell extracts. pqr activities for the reduction of 9,10-phenanthrenequinone and 4,5-pyrene- quinone were 1.40+/-0.13 and 0.12+/-0.01 micromol min(-1) mg-protein(-1), respectively. the exogenous catechols alizarin, anthrarobin, 2,3-dihydroxynaphthalene and escul ... | 2004 | 15549609 |
| mycobacterium pyrenivorans sp. nov., a novel polycyclic-aromatic-hydrocarbon-degrading species. | the taxonomic position of a polycyclic-aromatic-hydrocarbon-degrading bacterium, strain 17a3(t), isolated from contaminated soil was determined using a combination of phenotypic and genotypic properties. the isolate showed phenotypic properties that were diagnostic for species of the genus mycobacterium. comparative 16s rrna gene sequence analysis assigned 17a3(t) to the 16s rrna gene subgroup that contains mycobacterium aurum, mycobacterium austroafricanum, mycobacterium vaccae and mycobacteriu ... | 2004 | 15545477 |
| novel organization of genes in a phthalate degradation operon of mycobacterium vanbaalenii pyr-1. | mycobacterium vanbaalenii pyr-1 is capable of degrading polycyclic aromatic hydrocarbons (pahs) to ring cleavage metabolites. this study identified and characterized a putative phthalate degradation operon in the m. vanbaalenii pyr-1 genome. a putative regulatory protein (phtr) was encoded divergently with five tandem genes: phthalate dioxygenase large subunit (phtaa), small subunit (phtab), phthalate dihydrodiol dehydrogenase (phtb), phthalate dioxygenase ferredoxin subunit (phtac) and phthalat ... | 2004 | 15528661 |
| regio- and stereoselective metabolism of 7,12-dimethylbenz[a]anthracene by mycobacterium vanbaalenii pyr-1. | the degradation of 7,12-dimethylbenz[a]anthracene (dmba), a carcinogenic polycyclic aromatic hydrocarbon, by cultures of mycobacterium vanbaalenii pyr-1 was studied. when m. vanbaalenii pyr-1 was grown in the presence of dmba for 136 h, high-pressure liquid chromatography (hplc) analysis showed the presence of four ethyl acetate-extractable compounds and unutilized substrate. characterization of the metabolites by mass and nuclear magnetic resonance spectrometry indicated initial attack at the c ... | 2003 | 12839762 |
| molecular characterization of dioxygenases from polycyclic aromatic hydrocarbon-degrading mycobacterium spp. | polycyclic aromatic hydrocarbon (pah)-degrading genes nida and nidb that encode the alpha and beta subunits of the aromatic ring-hydroxylating dioxygenase have been cloned and sequenced from mycobacterium vanbaalenii pyr-1 [khan et al., appl. environ microbiol. 67 (2001) 3577-3585]. in this study, the presence of nida and nidb in 12 other mycobacterium or rhodococcus strains was investigated. initially, all strains were screened for their ability to degrade pahs by a spray plate method, and for ... | 2003 | 12829283 |
| classification of a polycyclic aromatic hydrocarbon-metabolizing bacterium, mycobacterium sp. strain pyr-1, as mycobacterium vanbaalenii sp. nov. | a polycyclic aromatic hydrocarbon (pah)-utilizing mycobacterium strain, pyr-1(t), was isolated from petroleum-contaminated estuarine sediments and has been shown by 16s rrna gene sequencing to be closely related to mycobacterium aurum atcc 23366(t) and mycobacterium vaccae atcc 15438(t). in this investigation, the 16s rdna, fatty acid methyl esters, dna-dna hybridization, pfge analysis of restriction-digested total genomic dna and biochemical tests were used to determine the taxonomic relationsh ... | 2002 | 12508859 |
| polycyclic aromatic hydrocarbon metabolic network in mycobacterium vanbaalenii pyr-1. | this study investigated a metabolic network (mn) from mycobacterium vanbaalenii pyr-1 for polycyclic aromatic hydrocarbons (pahs) from the perspective of structure, behavior, and evolution, in which multilayer omics data are integrated. initially, we utilized a high-throughput proteomic analysis to assess the protein expression response of m. vanbaalenii pyr-1 to seven different aromatic compounds. a total of 3,431 proteins (57.38% of the genome-predicted proteins) were identified, which include ... | 2011 | 21725022 |
| microbially mediated o-methylation of bisphenol a results in metabolites with increased toxicity to the developing zebrafish (danio rerio) embryo. | bisphenol a (bpa) is used in the manufacture of plastics, and has been identified in various environmental matrices, including human serum and breast milk. the prevalence of bpa in the environment and the potential exposure to humans underscores the need to more fully understand the fate of bpa in the environment and the resulting effects and toxicity to humans and other organisms. here we demonstrate that mycobacterium species, including mycobacterium vanbaalenii strain pyr-1, are able to o-met ... | 2011 | 21678910 |
| influence of single-walled carbon nanotubes on microbial availability of phenanthrene in sediment. | increasing production and use of single-walled carbon nanotubes (swcnt) will inevitably lead to release of these nanoparticles to aquatic ecosystems. similar to black carbon (bc) particles, swcnt have a high affinity for hydrophobic organic contaminants (hocs) and therefore the presence of swcnt in sediment may lead to altered bioavailability of hocs. we compared swcnt with biochar and charcoal on their effect on the microbial degradability of 0.05 mg kg(-1) (14)c-phenanthrene (phe) by mycobacte ... | 2011 | 21656161 |
| advances in the field of high-molecular-weight polycyclic aromatic hydrocarbon biodegradation by bacteria. | interest in understanding prokaryotic biotransformation of high-molecular-weight polycyclic aromatic hydrocarbons (hmw pahs) has continued to grow and the scientific literature shows that studies in this field are originating from research groups from many different locations throughout the world. in the last 10 years, research in regard to hmw pah biodegradation by bacteria has been further advanced through the documentation of new isolates that represent diverse bacterial types that have been ... | 2010 | 21255317 |
| biodegradation of pyrene in sand, silt and clay fractions of sediment. | microbial degradation is the dominant pathway for natural attenuation of pahs in environmental compartments such as sediments, which in turn depends on the bioavailability of pahs. the bioavailability of pahs has seldom been studied at the sediment particle size scale. we evaluated biodegradation of pyrene by mycobacterium vanbaalenii pyr-1 as a function of sediment particle sizes, and investigated the relationship between the rate of degradation on sand, silt and clay particles with their indiv ... | 2011 | 20714921 |
| pleiotropic and epistatic behavior of a ring-hydroxylating oxygenase system in the polycyclic aromatic hydrocarbon metabolic network from mycobacterium vanbaalenii pyr-1. | despite the considerable knowledge of bacterial high-molecular-weight (hmw) polycyclic aromatic hydrocarbon (pah) metabolism, the key enzyme(s) and its pleiotropic and epistatic behavior(s) responsible for low-molecular-weight (lmw) pahs in hmw pah-metabolic networks remain poorly understood. in this study, a phenotype-based strategy, coupled with a spray plate method, selected a mycobacterium vanbaalenii pyr-1 mutant (6g11) that degrades hmw pahs but not lmw pahs. sequence analysis determined t ... | 2014 | 25070740 |
| prevalence and distribution of non-tuberculous mycobacteria (ntm) in cattle, african buffaloes (syncerus caffer) and their environments in south africa. | it has been hypothesized that a variety of non-tuberculous mycobacteria (ntm) species to which livestock and wildlife species are naturally exposed induce broadly cross-reactive anti-mycobacterial immune responses which interfere with current standard diagnostic assays. non-tuberculous mycobacteria have also been implicated in mycobacterium bovis-specific immune responsiveness, hence potentially the development of tuberculosis. cattle and african buffaloes are both maintenance hosts of bovine tu ... | 2013 | 24171852 |
| characterization of a pyrene-degrading mycobacterium sp. strain ch-2. | mycobacterium sp strain ch-2 was isolated from a manufactured gas plant contaminated with polycyclic aromatic hydrocarbons (pahs) and was identified by analysis of 16s rdna sequences. strain ch-2 was capable of mineralizing 3- and 4- ring pahs, including phenanthrene, pyrene, and fluoranthene. in addition, strain ch-2 could utilize phenanthrene, pyrene and a wide range of alkanes as a sole carbon and energy source. primers based upon the sequences of the polycyclic aromatic hydrocarbon (pah) dio ... | 2008 | 18941994 |
| identification of compounds with potential antibacterial activity against mycobacterium through structure-based drug screening. | to identify novel antibiotics against mycobacterium tuberculosis, we performed a hierarchical structure-based drug screening (sbds) targeting the enoyl-acyl carrier protein reductase (inha) with a compound library of 154,118 chemicals. we then evaluated whether the candidate hit compounds exhibited inhibitory effects on the growth of two model mycobacterial strains: mycobacterium smegmatis and mycobacterium vanbaalenii. two compounds (ke3 and ke4) showed potent inhibitory effects against both mo ... | 2013 | 23600706 |
| identification of novel antimycobacterial chemical agents through the in silico multi-conformational structure-based drug screening of a large-scale chemical library. | the increasing prevalence of drug-resistant tuberculosis, which is resistant to effective multiple antibiotic, presents a major global health threat. the thymidine monophosphate kinase (tmpk) of mycobacterium tuberculosis (m. tuberculosis), which is an essential enzyme for the maintenance of the thymidine triphosphate pools, is considered an attractive target for the development of effective antibiotics against tuberculosis. in this study, we attempted to identify novel chemical compounds that s ... | 2013 | 23314046 |
| a polyomic approach to elucidate the fluoranthene-degradative pathway in mycobacterium vanbaalenii pyr-1. | mycobacterium vanbaalenii pyr-1 is capable of degrading a wide range of high-molecular-weight polycyclic aromatic hydrocarbons (pahs), including fluoranthene. we used a combination of metabolomic, genomic, and proteomic technologies to investigate fluoranthene degradation in this strain. thirty-seven fluoranthene metabolites including potential isomers were isolated from the culture medium and analyzed by high-performance liquid chromatography, gas chromatography-mass spectrometry, and uv-visibl ... | 2007 | 17449607 |
| [polymorphism of the bpha genes in bacteria destructing biphenyl/chlorinated biphenils]. | polychlorinated biphenyls (pcbs) are persistent organic pollutants. biphenyl 2,3-dioxygenase (bdo) is a key enzyme that determines the range of pcbs oxidized by a bacterial strain. bdo subunit α (bpha1) plays an essential role in substrate recognition and binding. the genes for dioxygenases that hydroxylate aromatic rings were screened and analyzed phylogenetically. genes found in biphenyl-oxidizing rhodococcus erythropolis strains g12a, b7b, and b106a proved to be similar to the published nucle ... | 2017 | 26299864 |
| mid-infrared spectroscopic assessment of nanotoxicity in gram-negative vs. gram-positive bacteria. | nanoparticles appear to induce toxic effects through a variety of mechanisms including generation of reactive oxygen species (ros), physical contact with the cell membrane and indirect catalysis due to remnants from manufacture. the development and subsequent increasing usage of nanomaterials has highlighted a growing need to characterize and assess the toxicity of nanoparticles, particularly those that may have detrimental health effects such as carbon-based nanomaterials (cbns). due to interac ... | 2014 | 24162371 |
| simultaneous biodegradation of polyaromatic hydrocarbons by a stenotrophomonas sp: characterization of nid genes and effect of surfactants on degradation. | a polyaromatic hydrocarbon degrading bacterium was isolated from a petroleum contaminated site and designated as stenotrophomonas sp. strain iitr87. it was found to utilize pyrene, phenanthrene and benzo(a)pyrene as sole carbon source, but not anthracene, chrysene and fluoranthene. gas chromatography and mass spectroscopy analysis resulted in identification of pyrene metabolites namely monohydroxypyrene, 4-oxa-pyrene-5-one, dimethoxypyrene and monohydroxyphenanthrene. southern hybridization usin ... | 2017 | 28148980 |
| a comparative intracellular proteomic profiling of pseudomonas aeruginosa strain asp-53 grown on pyrene or glucose as sole source of carbon and identification of some key enzymes of pyrene biodegradation pathway. | pseudomonas aeruginosa strain asp-53, isolated from a petroleum oil-contaminated soil sample, was found to be an efficient degrader of pyrene. pcr amplification of selected hydrocarbon catabolic genes (alkb gene, which encodes for monooxygenase, and the c12o, c23o, and pah-rhdα genes encoding for the dioxygenase enzyme) from the genomic dna of p. aeruginosa strain asp-53 suggested its hydrocarbon degradation potential. the gc-ms analysis demonstrated 30.1% pyrene degradation by p. aeruginosa str ... | 2017 | 28774858 |
| molecular characterization of a phenanthrene degradation pathway in mycobacterium vanbaalenii pyr-1. | mycobacterium vanbaalenii pyr-1 is capable of degrading a number of polycyclic aromatic hydrocarbons (pahs) to ring cleavage metabolites via multiple pathways. genes for the large and small subunits of a pyrene dioxygenase, nida and nidb, respectively, were previously identified in m. vanbaalenii pyr-1 [appl. environ. microbiol. 67 (2001) 3577]. a library of the m. vanbaalenii pyr-1 genome was constructed in a fosmid vector to identify additional genes involved in pah degradation. twelve fosmid ... | 2004 | 15313184 |
| isolation and characterization of polycyclic aromatic hydrocarbon-degrading mycobacterium isolates from soil. | bioremediation of soils contaminated with wood preservatives containing polycyclic aromatic hydrocarbons (pahs) is desired because of their toxic, mutagenic, and carcinogenic properties. creosote wood preservative-contaminated soils at the champion international superfund site in libby, montana currently undergo bioremediation in a prepared-bed land treatment unit (ltu) process. microbes isolated from these ltu soils rapidly mineralized the (14)c-labeled pah pyrene in the ltu soil. gram staining ... | 2004 | 15107954 |
| degradation of benzo[a]pyrene by mycobacterium vanbaalenii pyr-1. | metabolism of the environmental pollutant benzo[a]pyrene in the bacterium mycobacterium vanbaalenii pyr-1 was examined. this organism initially oxidized benzo[a]pyrene with dioxygenases and monooxygenases at c-4,5, c-9,10, and c-11,12. the metabolites were separated by reversed-phase high-performance liquid chromatography (hplc) and characterized by uv-visible, mass, nuclear magnetic resonance, and circular dichroism spectral analyses. the major intermediates of benzo[a]pyrene metabolism that ha ... | 2004 | 14711661 |
| formal description of mycobacterium neglectum sp. nov. and mycobacterium palauense sp. nov., rapidly growing actinobacteria. | the taxonomic positions of two fast growing mycobacteria (cect 8778t and cect 8779t) were established using a polyphasic approach. the strains were shown to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus mycobacterium. multi-locus sequence analyses (mlsa) show that strain cect 8778t forms a well-supported clade together with the type strains of mycobacterium aurum, mycobacterium austroafricanum and mycobacterium vanbaalenii while stra ... | 2018 | 29404824 |
| spectrochemical analyses of growth phase-related bacterial responses to low (environmentally-relevant) concentrations of tetracycline and nanoparticulate silver. | exposure to environmental insults generally occurs at low levels, making it challenging to measure bacterial responses to such interactions. additionally, microbial behaviour and phenotype varies in differing bacterial types or growth phases, likely giving rise to growth- or species-specific responses to environmental stimuli. the present study applied a spectrochemical tool, infrared (ir) spectral interrogation coupled with multivariate analysis, to investigate the growth- and species-specific ... | 2018 | 29327006 |
| microbial transformation of multiwalled carbon nanotubes by mycobacterium vanbaalenii pyr-1. | carbonaceous nanomaterials are widely used in industry and consumer products, but concerns have been raised regarding their release into the environment and subsequent impacts on ecosystems and human health. although many efforts have been devoted to understanding the environmental fate of carbonaceous nanomaterials, information about their microbial transformation is still rare. in this study, we found that within 1 month a polycyclic aromatic hydrocarbon-degrading bacterium, mycobacterium vanb ... | 2017 | 28081361 |
| protein structure. crystal structure of a mycobacterial insig homolog provides insight into how these sensors monitor sterol levels. | insulin-induced gene 1 (insig-1) and insig-2 are endoplasmic reticulum membrane-embedded sterol sensors that regulate the cellular accumulation of sterols. despite their physiological importance, the structural information on insigs remains limited. here we report the high-resolution structures of mvins, an insig homolog from mycobacterium vanbaalenii. mvins exists as a homotrimer. each protomer comprises six transmembrane segments (tms), with tm3 and tm4 contributing to homotrimerization. the s ... | 2015 | 26160948 |
| oil biodegradation and oil-degrading microbial populations in marsh sediments impacted by oil from the deepwater horizon well blowout. | to study hydrocarbon biodegradation in marsh sediments impacted by macondo oil from the deepwater horizon well blowout, we collected sediment cores 18-36 months after the accident at the marshes in bay jimmy (upper barataria bay), louisiana, united states. the highest concentrations of oil were found in the top 2 cm of sediment nearest the waterline at the shorelines known to have been heavily oiled. although petroleum hydrocarbons were detectable, macondo oil could not be identified below 8 cm ... | 2015 | 26091189 |
| dynamic response of mycobacterium vanbaalenii pyr-1 to bp deepwater horizon crude oil. | we investigated the response of the hydrocarbon-degrading mycobacterium vanbaalenii pyr-1 to crude oil from the bp deepwater horizon (dwh) spill, using substrate depletion, genomic, and proteome analyses. m. vanbaalenii pyr-1 cultures were incubated with bp dwh crude oil, and proteomes and degradation of alkanes and polycyclic aromatic hydrocarbons (pahs) were analyzed at four time points over 30 days. gas chromatography-mass spectrometry (gc-ms) analysis showed a chain length-dependent pattern ... | 2015 | 25888169 |
| functional robustness of a polycyclic aromatic hydrocarbon metabolic network examined in a nida aromatic ring-hydroxylating oxygenase mutant of mycobacterium vanbaalenii pyr-1. | in this study, we obtained over 4,000 transposon mutants of mycobacterium vanbaalenii pyr-1 and analyzed one of the mutants, 8f7, which appeared to lose its ability to degrade pyrene while still being able to degrade fluoranthene. this mutant was identified to be defective in nida, encoding an aromatic ring-hydroxylating oxygenase (rho), known to be involved in the initial oxidation step of pyrene degradation. when cultured with pyrene as a sole source of polycyclic aromatic hydrocarbon (pah), h ... | 2012 | 22407691 |