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sinorhizobium medicae sp. nov., isolated from annual medicago spp.the taxonomic position of isolates of a new genomic species (designated genomic species 2) obtained from several annual medicago species and originating from different geographical locations was established through the results of phenotypic tests (including the results of auxanographic and biochemical tests and symbiotic properties) and 16s rrna phylogenetic inferences. a comparison of the complete 16s rrna sequence of a representative of genomic species 2 (strain a 321t [t = type strain]) with ...19968863426
sinorhizobium teranga bv. acaciae ors1073 and rhizobium sp. strain ors1001, two distantly related acacia-nodulating strains, produce similar nod factors that are o carbamoylated, n methylated, and mainly sulfated.we have determined the structures of nod factors produced by strains representative of sinorhizobium teranga bv. acaciae and the so-called cluster u from the rhizobium loti branch, two genetically different symbionts of particular acacia species. compounds from both strains were found to be similar, i.e., mainly sulfated, o carbamoylated, and n methylated, indicating a close relationship between host specificity and nod factor structure, regardless of the taxonomy of the bacterial symbiont.19979139935
three phylogenetic groups of noda and nifh genes in sinorhizobium and mesorhizobium isolates from leguminous trees growing in africa and latin america.the diversity and phylogeny of noda and nifh genes were studied by using 52 rhizobial isolates from acacia senegal, prosopis chilensis, and related leguminous trees growing in africa and latin america. all of the strains had similar host ranges and belonged to the genera sinorhizobium and mesorhizobium, as previously determined by 16s rrna gene sequence analysis. the restriction patterns and a sequence analysis of the noda and nifh genes divided the strains into the following three distinct grou ...19989464375
characterization of tropical tree rhizobia and description of mesorhizobium plurifarium sp. nov.a collection of strains isolated from root nodules of acacia species in senegal was analysed previously by electrophoresis of total cell protein, auxanographic tests, rrna-dna hydridization, 16s rrna gene sequencing, dna base composition and dna-dna hybridization [de lajudie, p., willems, a., pot, b. & 7 other authors (1994). int j syst bacteriol 44, 715-733]. strains from acacia were shown to belong to two groups, sinorhizobium terangae, and a so-called gel electrophoretic cluster u, which also ...19989731275
sinorhizobium arboris sp. nov. and sinorhizobium kostiense sp. nov., isolated from leguminous trees in sudan and kenya.sds-page of total bacterial proteins was applied to the classification of 25 sudanese and five kenyan strains isolated from the root nodules of acacia senegal and prosopis chilensis. twenty strains were also studied by multilocus enzyme electrophoresis (mlee) and the whole 16s rrna gene was sequenced from two strains representing the two major clusters. these results, together with the previously reported numerical taxonomy analysis, pulsed-field gel electrophoresis studies, dna-dna dot-blot hyb ...199910555313
analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system.previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ...200010758890
the diversity of rhizobia nodulating beans in northwest argentina as a source of more efficient inoculant strains.the common bean (phaseolus vulgaris l.) is cultivated widely in central and south america and particularly in the northwest of argentina. in order to describe the diversity of the common bean nodulating rhizobial population from the bean producing area in northwest argentina (nwa), a collection of about 400 isolates of common beans recovered from nodules and soil samples from nwa were characterized by using nifh-pcr, analysis of genes coding for 16s rrna and nodc, and rep-fingerprinting, respect ...200111566389
arbuscular mycorrhizal symbiosis changes the colonization pattern of acacia tortilis spp. raddiana rhizosphere by two strains of rhizobia.the aim of the study was to assess the effect of the mycorrhizosphere of a. tortillis spp. raddiana mycorrhized with glomus intraradices on the root nodulation by sinorhizobium terangae (ors 1009) and/or mesorhizobium plurifarium (ors 1096) in two different culture substrates (sandy soil and sand). the endomycorrhizal fungus only stimulated plant growth in the sandy soil. moreover, arbuscular mycorrhizal infection enhanced the nodulation process in both culture substrates. beside the stimulatory ...200312545310
the genus name ensifer casida 1982 takes priority over sinorhizobium chen et al. 1988, and sinorhizobium morelense wang et al. 2002 is a later synonym of ensifer adhaerens casida 1982. is the combination "sinorhizobium adhaerens" (casida 1982) willems et al. 2003 legitimate? request for an opinion.the synonymy of the genera ensifer and sinorhizobium was recently reported, but it was proposed that the later-named genus, sinorhizobium, take priority in nomenclature. there is no justification in the international code of nomenclature of bacteria (prokaryotes) for this step; ensifer is the correct name of the genus, with ensifer adhaerens as the type species. species previously allocated to sinorhizobium are here proposed as the new combinations ensifer arboris, ensifer fredii, ensifer kostie ...200314657154
the sesbania root symbionts sinorhizobium saheli and s. teranga bv. sesbaniae can form stem nodules on sesbania rostrata, although they are less adapted to stem nodulation than azorhizobium caulinodans.sesbania species can establish symbiotic interactions with rhizobia from two taxonomically distant genera, including the sesbania rostrata stem-nodulating azorhizobium sp. and azorhizobium caulinodans and the newly described sinorhizobium saheli and sinorhizobium teranga bv. sesbaniae, isolated from the roots of various sesbania species. a collection of strains from both groups were analyzed for their symbiotic properties with different sesbania species. s. saheli and s. teranga bv. sesbaniae st ...199716535538
ensifer mexicanus sp. nov. a new species nodulating acacia angustissima (mill.) kuntze in mexico.a new lineage of ensifer nodulating the american legume acacia angustissima in the tropical forest of chiapas and morelos, mexico is described. bacteria were identified as ensifer with ssb or nolr specific primers. phylogenetic analysis with partial sequences of the five chromosomal genes gyra, nolr, reca, rpob and rrs revealed that this new lineage is related to african ensifer terangae. the results of total dna-dna hybridization and selected phenotypic tests among the a. angustissima strains a ...200717293074
rhizobia with different symbiotic efficiencies nodulate acaciella angustissima in mexico, including sinorhizobium chiapanecum sp. nov. which has common symbiotic genes with sinorhizobium mexicanum.bacteria from nodules of the legume acaciella angustissima native to the south of mexico were characterized genetically and their nodulation and competitiveness were evaluated. phylogenetic studies derived from rpob gene sequences indicated that a. angustissima is nodulated by sinorhizobium mexicanum, rhizobium tropici, mesorhizobium plurifarium and agrobacterium tumefaciens and by bacteria related to sinorhizobium americanum, sinorhizobium terangae, rhizobium etli and rhizobium gallicum. a new ...200919120461
phenotypic, molecular and symbiotic characterization of the rhizobial symbionts of desmanthus paspalaceus (lindm.) burkart that grow in the province of santa fe, argentina.desmanthus paspalaceus (lindm.) burkart belongs to the d. virgatus complex, subfamily mimosoidae. the known potential as livestock fodder of several of these legumes prompted us to undertake a phenotypic, molecular, and symbiotic characterization of the d. paspalaceus symbionts in the santa fe province, argentina. the rhizobia collected--containing isolates with different abiotic-stress tolerances--showed a remarkable genetic diversity by pcr fingerprinting, with 11 different amplification profi ...201425153989
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