| porcine cytomegalovirus (pcmv) in early gestation. | following intranasal exposure to pcmv at or within 48 h of coitus transplacental infection occurred in two groups of gilts. five out of 22 embryos were infected in the first group but only 2 out of 63 in the second. a more rapid immune response as measured by circulating antibody was probably instrumental in abrogating infection in the second group. in the infected embryos the virus localized in leptomeningeal cells, hepatic sinusoidal cells, peritoneal macrophages, periosteal cells and occasion ... | 1988 | 2845635 |
| replication of porcine cytomegalovirus in the 19-pft cell line. | replication of porcine cytomegalovirus was examined in fibroblast- and epithelial-like cell lines of the 19-pft cell line derived from pig fallopian tube. the virus grew well in the fibroblast-like cell line than the epithelial-like cell line. cytomegalic cytopathic effects of the virus were clearly observed under the microscope after dispersion of the infected cell culture by trypsin-versene and it was demonstrated that cytomegalic cytopathic effects could be used for infectivity titration. int ... | 1992 | 1335761 |
| electron microscopy of porcine cytomegalovirus in pig lung macrophage cultures. | | 1979 | 231358 |
| isolation and experimental infection with porcine cytomegalovirus. | | 1977 | 195421 |
| experimental transplacental transmission of porcine cytomegalovirus. | six serologically negative sows were infected by intranasal instillation of porcine cytomegalovirus (pcmv) between 31 and 85 days of pregnacy. four sows showed an afebrile anorexia and lethargy 14-25 days after infection and all 6 developed significant increases in indirect immunofluorescent (iif) antibody titres within 35 days. virus was recovered from nasal and/or cervical swabs from 2 sows during life and from lung macrophage cultures after death. at term the sows were killed and their fetuse ... | 1977 | 191522 |
| cytomegalovirus excretion in gnotobiotic pigs. | germ-free piglets were infected intranasally with porcine cytomegalovirus (pcmv) at 1 day (group a) or 3 weeks of age (group b). viraemia and virus excretion by the nasal, pharyngeal and conjunctival routes was studied up to the time of death or to 12 weeks. virus was also sought in tissues at death or at slaughter, as well as in a few urine samples. viraemia was detected in group a between days 5 and 19 after infection and in group b between days 14 and 16 inclusive. the chief route of virus ex ... | 1976 | 185292 |
| relationship of porcine cytomegalovirus and b bronchiseptica to atrophic rhinitis in gnotobiotic piglets. | both porcine cytomegalovirus (pcmv) and bordetella bronchiseptica produced rhinitis and pneumonia when inoculated intranasally into young gnotobiotic pigs. with pcmv the nasal lesions were confirmed to the lamina propria, while bordetella produced atrophy of the turbinate bones and hyperplasia and degeneration of the nasal epithelium. some exacerbation of the lesions was observed in the nasal mucosa of pigs given both agents, but the degree of bone atrophy was not increased. | 1976 | 176765 |
| the behaviour of porcine cytomegalovirus in commercial pig herds. | a longitudinal, virological and serological study of pigs in two herds with respiratory disease showed that infection by porcine cytomegalovirus (pcmv) was universal in both. virus excretion usually began when piglets were between 3 and 6 weeks of age and reached a maximum between 5 and 8 weeks; it was usually no longer detectable at 11-12 weeks. antibody demonstrable in indirect immunofluorescence (iif) tests was present to moderate or high titre in all piglets at 2-3 weeks. this was presumed t ... | 1976 | 173754 |
| pathologic changes in pigs with prednisolone-induced recrudescence of herpesvirus infection. | in pigs inoculated with aujeszky's disease virus (adv), recrudescence of herpesvirus infection was induced by daily administration of 1,000 mg of prednisolone for 5 days at 2 (group a) or 5 (group b) months after the primary infection. at necropsy in group a pigs, adv was recovered from nasal secretions 3 to 9 days after prednisolone treatment initiation and from the brain cortex 10 days after treatment initiation; adv was not recovered from group b pigs. in pigs of both groups killed 10 days af ... | 1985 | 2992328 |
| superinfection with porcine cytomegalovirus initiating transplacental infection. | four sows with circulating antibody were exposed to porcine cytomegalovirus. virus was detected in 8 of 24 foetuses by immunofluorescence and/or virus isolation from 2 sows with low levels of antibody. in 6 of the infected foetuses, the virus was in capillary endothelium and macrophages of the lung, and was associated with interlobular oedema in 2 of these. virus was also detected in the nasal mucosa, spleen and brain. the majority of the virus-positive foetuses were grossly normal. | 1988 | 2836992 |
| morphologic study of inclusions in tissues from pigs inoculated with cytomegalovirus. | six 5-day-old gnotobiotic pigs inoculated intranasally with the j-1 strain of porcine cytomegalovirus (pcmv) were euthanatized within 9 to 18 days after inoculation. all pigs developed a subclinical form of pcmv infection and had characteristic intranuclear inclusions in all tissues. morphologically, 2 types of inclusions were observed. one was a small herpetic intranuclear inclusion in reticuloendothelial cells, which contained a few nucleocapsids in the nucleus. the other was a typical cytomeg ... | 1987 | 2821852 |
| characterization of the dna polymerase loci of porcine cytomegaloviruses from diverse geographic origins. | porcine cytomegalovirus (pcmv) is an undesired pathogen in pigs intended for use as organ donors in xenotransplantation. in the present work, we characterized the first set of genes of pcmv. from a german isolate, the dna polymerase (dpol) locus was amplified and two complete open reading frames (orf) as well as two partial orfs including the complete dpol gene and the 3'-end of the glycoprotein gb gene were sequenced. the deduced amino acid sequences showed the highest identities with the respe ... | 2000 | 11129643 |
| xenotransplantation and the potential risk of xenogeneic transmission of porcine viruses. | the clinical success of allotransplantation and the shortage of donor organs have led to a proposal for the use of animal organs as alternative therapeutic materials for humans. in that regard, swine are preferable to non-human primates as a source of donor organs. while applications for clinical trials for xenotransplantation have not yet been received in canada, several trials have already been authorized in the united states. a major concern, however, is the potential for xenogeneic transmiss ... | 2000 | 11041495 |
| analysis of porcine cytomegalovirus dna polymerase by consensus primer pcr. | we used a consensus primer pcr method to amplify a region of herpesviral dna-directed dna polymerase gene using degenerate primers for initial characterization of the porcine cytomegalovirus (pcmv) genome. the sequence of the pcr product from pcmv dna template and its alignment with other herpesvirus dna polymerase counterparts showed that both conserved amino acid residues and conservative amino acid substitutions are in parallel. phylogenetic analysis revealed that pcmv should be included in t ... | 1999 | 10593586 |
| pcr assay for detecting porcine cytomegalovirus. | this is the first published report of a pcr assay for detecting porcine cytomegalovirus (pcmv), the causative agent of inclusion body rhinitis in pigs. the dna to be tested was extracted directly from lungs and nasal scrapings of pigs with various clinical syndromes. fifty-nine percent (74 of 126) of tested pigs with various clinical syndromes were found to be pcr positive for pcmv. it is hoped that veterinary diagnostic laboratories will benefit by using this pcr assay for routine testing and s ... | 1999 | 10523598 |
| development of a pcr system for porcine cytomegalovirus detection and determination of the putative partial sequence of its dna polymerase gene. | after pcr amplification with conservative cytomegalovirus primers, a 520 nucleotide putative partial sequence of the dna polymerase gene of porcine cytomegalovirus (pcmv) was determined. sequence comparison revealed homology to dna polymerase genes from various beta herpes viruses and a dendrogram was constructed depicting the relationship of pcmv to other members of the herpesviridae family. the dendrogram indicates that pcmv is indeed a beta herpes virus that is more closely related to human h ... | 1999 | 10487654 |
| detection of two novel porcine herpesviruses with high similarity to gammaherpesviruses. | evidence for the existence of porcine gammaherpesviruses was obtained by pcr and sequence analysis. initially, samples of peripheral blood mononuclear cells (pbmc), spleens, lungs, kidneys and livers of pigs from germany and spain were tested with a pcr assay which targets conserved regions of the herpesvirus dna polymerase gene with degenerate and deoxyinosine-substituted primers. amplicons of identical sequence were obtained from one spleen and two pbmc samples. this sequence showed a high per ... | 1999 | 10211967 |
| evaluation of porcine cytomegalovirus as a potential zoonotic agent in xenotransplantation. | | 1999 | 10083402 |
| histochemical and lectinhistochemical studies on nasal mucosa of pigs with or without respiratory diseases. | histochemical and lectinhistochemical examinations were carried out on nasal mucosa of pigs with or without respiratory diseases. as the results, both acid and neutral mucins coexisted in nasal mucosa of normal pigs while acid sialomucins were mainly observed in nasal mucosa of pigs infected with bordetella bronchiseptica and/or pasteurella multocida. lectinhistochemistry revealed that the nasal epithelial cells of normal pigs were rich in n-acetylgalactosamine, fucose and n-acetyl-glucosamine r ... | 1998 | 9795904 |
| serological relationship among porcine cytomegalovirus japanese isolates and a uk isolate. | we isolated porcine cytomegaloviruses (pcmvs) from lung samples of fattening pigs collected in the slaughter houses of 4 prefectures of japan. seven isolates were obtained and used for a comparison of serological characteristics by elisa. j1, the first field isolate in japan, and b6 which was isolated in the uk were also used in the study. the serological relationships between the isolates were analysed by the method of archetti and horsfall. of1 showed serological differences with chiba2 and hi ... | 1998 | 9492369 |
| herpesviral abortion in domestic animals. | abortion or neonatal disease may follow infection with several alpha, beta and gamma-herpesviruses. the alpha-herpesvirus, equid herpesvirus-1 (ehv-1), causes single or epizootic abortions or neonatal deaths in equids, and the closely related virus ehv-4 causes sporadic equine abortions. in cattle, the alpha-herpesviruses, bovine herpesvirus-1 (infectious bovine rhinotracheitis virus) and bovine herpesvirus-5 (bovine encephalitis virus), and a gamma-herpesvirus, bovine herpesvirus-4, have all be ... | 1997 | 9232116 |
| influence of 12-o-tetradecanoylphorbol 13-acetate on replication of porcine cytomegalovirus in the 19-pft-f cell line. | severe cytopathic effects, formation of swollen cells and small syncitia, appeared in 19-pft-f cell cultures at 2 days when a large amount of the of1 strain of porcine cytomegalovirus (pcmv) was inoculated and cultured in media containing 5, 10, 30 and 60 ng per ml of 12-o-tetradecanoylphorbol 13-acetate (tpa). characteristic focal clusters of swollen cells appeared on the 19-pft-f monolayers 3 to 4 days after inoculation with a small amount of the virus. cells in the foci contained specific flu ... | 1996 | 8777236 |
| application of enzyme-linked immunosorbent assay for the seroepizootiological survey of antibodies against porcine cytomegalovirus. | enzyme-linked immunosorbent assay (elisa) for the detection of antibodies against porcine cytomegalovirus (pcmv) was developed using the of-1 strain of pcmv as an antigen. results of the elisa were compared to those of indirect fluorescent antibody (ifa) and serum neutralization (sn) tests. elisa and ifa test were found to be extremely sensitive more than sn test. all of 11 tested sera were highly reactive in both elisa and ifa test, but 6 of them were antibody-negative in the sn test. the retro ... | 1993 | 8395226 |
| detection of the antibodies against porcine cytomegalovirus from whole blood collected on the blood sampling paper. | the usefulness of blood sampling paper for the detection of antibodies against porcine cytomegalovirus (pcmv) by enzyme-linked immunosorbent assay (elisa) was examined. the pcmv antibody titer of whole blood collected on the paper agreed with that of serum and the absorbances of the corresponding samples were well correlated (r = 0.88). blood was collected from 26 piglets in the field on the blood sampling paper and elisa was performed. the maternal pcmv antibody of these piglets decreased durin ... | 1994 | 8204754 |
| enzyme linked immunosorbent assay (elisa) for the detection of antibodies to porcine cytomegalovirus. | the elisa and indirect immunofluorescence test were compared on 56 porcine sera which were tested for antibodies to porcine cytomegalovirus. viral antigens were prepared in cells of a pig fallopian tube line. the elisa was found to be a sensitive reproducible and practical test to measure specific antibodies to this infection. | 1982 | 6284328 |
| electron microscopy of porcine cytomegalovirus. viral crystalline arrays and viral forms in the nasal mucosa of piglets. | | 1973 | 4354237 |
| quantitation of porcine cytomegalovirus in pig tissues by pcr. | a quantitative-competitive pcr for the quantification of porcine cytomegalovirus (pcmv) was developed. the virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from <10 to 97 genome copies/microg of dna. this assay will have significant utility for studying the activation and replication of pcmv and in swine models for allo- and xenotransplantation. | 2001 | 11230447 |
| identification of the porcine cytomegalovirus major capsid protein gene. | a major capsid protein (mcp) gene homologue of porcine cytomegalovirus (pcmv) was identified. sequence analysis indicated that the pcmv mcp gene is 4,026 nucleotides in length encoding a protein of 1,341 amino acid residues. the predicted molecular weight of the pcmv mcp is 151,456 da, equivalent to those of other herpesvirus mcp counterparts. phylogenetic analysis using herpesviral mcp gene sequences confirmed that pcmv is a betaherpesvirus with higher homology with human herpesvirus-6 and -7 t ... | 2001 | 11459006 |
| identification and sequence analysis of the glycoprotein b gene of porcine cytomegalovirus. | porcine cytomegalovirus (pcmv) is one of the pathogens that should be eliminated from pigs intended for use as organ donors in xenotransplantation. for this purpose, reliable diagnostic test systems are needed. to provide a basis for this goal and to analyse the evolutionary relationships of pcmv within the herpesvirus family, the putative glycoprotein b (gb) gene of pcmv was identified by assuming gene colinearity and a relative conservation of nucleotide sequences in comparison with closely re ... | 2001 | 11778702 |
| correlation of biochemical and hematological changes with graft failure following pig heart and kidney transplantation in baboons. | we have explored biochemical and hematologic parameters that might indicate acute humoral xenograft rejection (ahxr) following pig organ transplantation in baboons. baboons (n = 15) received an immunosuppressive regimen, and underwent a miniature swine or hdaf kidney (group 1, n = 6) or heart (group 2, n = 7) transplantation. control baboons (group 3, n = 2) received the immunosuppressive regimen without organ transplantation. blood chemistry and hematologic parameters were measured daily. baboo ... | 2003 | 14629281 |
| novel herpesviruses of suidae: indicators for a second genogroup of artiodactyl gammaherpesviruses. | five novel herpesviruses were identified in suid species from africa (common warthog, phacochoerus africanus) and south-east asia (bearded pig, sus barbatus; babirusa, babyrousa babyrussa) by detection and analysis of their dna polymerase genes. three of the novel species, p. africanus cytomegalovirus 1, p. africanus lymphotropic herpesvirus 1 (pafrlhv-1) and s. barbatus lymphotropic herpesvirus 1 (sbarlhv-1), were closely related to known beta- (porcine cytomegalovirus) and gammaherpesviruses [ ... | 2004 | 15039528 |
| reactivation of porcine cytomegalovirus through allogeneic stimulation. | reactivation of latent porcine cytomegalovirus after coculture of peripheral blood mononuclear cells (pbmcs) from pigs with different genetic backgrounds was investigated. nine of 10 allogeneic coculture pairs were pcr (dna) positive, whereas 7 coculture pairs had porcine cytomegalovirus (pcmv) rna, an indication of virus replication. the cell subpopulations harboring pcmv were monocytes and cd8+ t cells. | 2004 | 15071041 |
| activation of porcine cytomegalovirus, but not porcine lymphotropic herpesvirus, in pig-to-baboon xenotransplantation. | tissue-invasive disease due to porcine cytomegalovirus (pcmv) has been demonstrated after pig-to-baboon solid-organ xenotransplantation. porcine lymphotropic herpesvirus (plhv)-1 is associated with b cell proliferation and posttransplant lymphoproliferative disorder after allogeneic bone marrow transplantation in swine but has not been observed in pig-to-primate xenotransplantation. activation of pcmv and plhv-1 was investigated in 22 pig-to-baboon xenotransplants by use of quantitative polymera ... | 2004 | 15116299 |
| susceptibility of porcine cytomegalovirus to antiviral drugs. | re-activation of porcine cytomegalovirus (pcmv) in the xenograft has been reported in pig-to-baboon models of xenotransplantation and is associated with invasive disease and consumptive coagulopathy. if xenotransplantation of porcine organs into human recipients is to proceed, donor organs will have to be free from a wide range of infectious agents including pcmv. however, it is prudent to characterize the antiviral susceptibility of this virus. we therefore investigated the effect of selected a ... | 2004 | 15117919 |
| xenotransplantation: infectious risk revisited. | xenotransplantation is a possible solution for the shortage of tissues for human transplantation. multiple hurdles exist to clinical xenotransplantation, including immunologic barriers, metabolic differences between pigs--the source species most commonly considered--and humans, and ethical concerns. since clinical trials were first proposed almost 10 years ago, the degree of risk for infection transmitted from the xenograft donor to the recipient has been extensively investigated. a number of po ... | 2004 | 15307825 |
| herpesvirus infections in xenotransplantation: pathogenesis and approaches. | infectious risk remains an important consideration in the clinical application of xenotransplantation. vascularized xenografts create unique immunological niches in which bidirectional transmission of pathogens between donor and recipient may occur. enhanced replication of many pathogens is stimulated by the immune responses induced by transplantation and by the immune suppression used to prevent graft rejection. herpesviruses are the prototype viruses that are activated during immunosuppression ... | 2004 | 15479457 |
| early weaning of piglets fails to exclude porcine lymphotropic herpesvirus. | xenotransplantation using pigs as source species carries a risk for the activation of latent herpesviruses from the porcine donor and potential transmission to the recipient. in pig-to-baboon xenotransplantation, activation of porcine cytomegalovirus (pcmv) has been associated with xenograft injury and an increased incidence of consumptive coagulopathy and graft loss. activation of porcine lymphotropic herpesvirus (plhv)-1 was not observed in pig-to-baboon solid organ xenotransplantation, but wa ... | 2005 | 15598274 |
| reduction of consumptive coagulopathy using porcine cytomegalovirus-free cardiac porcine grafts in pig-to-primate xenotransplantation. | xenotransplantation using pigs as the source species for organs carries a potential risk for transmission and activation of porcine herpesviruses. activation of porcine cytomegalovirus (pcmv) in pig-to-baboon xenotransplantation is associated with xenograft injury and possibly an increased incidence of consumptive coagulopathy (cc). | 2004 | 15599308 |
| multiplex pcr for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs. | the use of porcine organs is being developed as a means to alleviate the shortage of human organs for transplantation. recommendations have been published for the microbiological specifications of organ-source pigs to reduce the possibility of a microorganism from pigs being inadvertently transferred to the recipient of the xenograft. the pseudorabies virus (prv), porcine cytomegalovirus (pcmv), and porcine circovirus (pcv) are infectious agents in pigs that are considered to be of significance ... | 2007 | 17034871 |
| [monitoring of infectious diseases among wild boars]. | sixty-seven serum samples and 43 pathological material samples from wild boars, taken in 5 regions of russia and in the kharkov region of the ukraine in 2002 to 2005 were studied. wild boars in some regions of russia were shown to be carriers of aujeszky's disease virus, porcine parvovirus, porcine circovirus type 2, lymphotropic herpesvirus-1, porcine cytomegalovirus, mycoplasma hyopneumoniae and pasteurella multocida. the classical swine fever (csf) virus genome was detected by polymerase chai ... | 2007 | 17601049 |
| characterization of porcine ul16-binding protein 1 endothelial cell surface expression. | natural killer (nk) cells participate in the immune response against solid organ allo- and xenografts and are tightly regulated through signals mediated by inhibiting and activating receptors expressed on their cell surface. human nk cytotoxicity against porcine endothelial cells (pec) is mediated by the interaction of the activating human nk receptor hnkg2d and its corresponding ligand on pec, porcine ul-16 binding protein 1 (pulbp1). the aim of the present study was to characterize the regulat ... | 2008 | 18447887 |
| human fibroblasts are permissive for porcine cytomegalovirus in vitro. | xenotransplantation with pig organs is being considered to alleviate donor organ shortages; however, the risk of introducing porcine viruses into humans is heightened in this setting. the goal of this study was to determine the infectious potential of porcine cytomegalovirus (pcmv), a xenozoonotic virus of interest, in human fibroblasts in vitro. | 2008 | 18622293 |
| absence of transmission of potentially xenotic viruses in a prospective pig to primate islet xenotransplantation study. | shortage of human donor organs for transplantation has prompted usage of animals as an alternative donor source. pigs are the most acceptable candidate animals but issues of xenozoonoses remain. despite careful monitoring of designated pathogen free pigs there is still a risk that their tissues may carry infectious agents. thus xenotransplantation requires extensive pre-clinical study on safety of the graft especially for those viruses that are either potentially oncogenic and/or immunosuppressi ... | 2008 | 18814261 |
| absence of replication of porcine endogenous retrovirus and porcine lymphotropic herpesvirus type 1 with prolonged pig cell microchimerism after pig-to-baboon xenotransplantation. | porcine endogenous retrovirus (perv), porcine cytomegalovirus (pcmv), and porcine lymphotropic herpesvirus (plhv) are common porcine viruses that may be activated with immunosuppression for xenotransplantation. studies of viral replication or transmission are possible due to prolonged survival of xenografts in baboon recipients from human decay-accelerating factor transgenic or alpha-1,3-galactosyltransferase gene knockout miniature swine. ten baboons underwent xenotransplantation with transgeni ... | 2008 | 18829759 |
| reduced efficacy of ganciclovir against porcine and baboon cytomegalovirus in pig-to-baboon xenotransplantation. | in pig-to-baboon xenotransplantation, porcine cytomegalovirus (pcmv) causes viremia, consumptive coagulopathy, and tissue-invasive disease. baboon cytomegalovirus (bcmv) is associated with invasive disease in xenograft recipients. the efficacy of prophylaxis with intravenous ganciclovir (gcv) was studied for prevention of pcmv and bcmv infections in pig-to baboon xenotransplantation. gcv prophylaxis did not alter the incidence of bcmv activation in recipients, but reduced the amount of virus in ... | 2003 | 12919084 |
| exogenous porcine viruses. | porcine organs, cells and tissues provide a viable source of transplants in humans, though there is some concern of public health risk from adaptation of swine infectious agents in humans. limited information is available on the public health risk of many exogenous swine viruses, and reliable and rapid diagnostic tests are available for only a few of these. the ability of several porcine viruses to cause transplacental fetal infection (parvoviruses, circoviruses, and arteriviruses), emergence or ... | 2003 | 12934944 |
| xenogeneic transplantation of porcine islets: an overview. | the extreme demand for human organs or tissues for transplantation has driven the search for viable alternatives. pigs are considered a possible source of tissue for a number of reasons including shared physiology, plentiful supply, short gestation, and, more recently, the generation of transgenic animals. porcine islets show promise as a source of islets for the treatment of type 1 diabetes mellitus. porcine islets regulate glucose levels in the same physiologic range as humans, and porcine ins ... | 2003 | 14508349 |
| the production of transgenic pigs for potential use in clinical xenotransplantation: microbiological evaluation. | debate over the infection hazards of pig-to-human xenotransplantation has focused mainly on the porcine endogenous retroviruses (perv). however, hazards of exogenous infectious agents possibly associated with the xenograft have also been evaluated (xenotransplantation 2000; 7: 143). we report the results of a health monitoring program demonstrating the exclusion of more than 80 potential pathogens from nine cohorts of pigs reared in a high welfare bioexclusion facility as potential xenograft sou ... | 2002 | 11983017 |
| porcine cytomegalovirus in pigs being bred for xenograft organs: progress towards control. | in human medicine, human cytomegalovirus (hcmv) is readily transmitted by organ transplant causing end-organ disease and triggering graft rejection in recipients. because of a chronic shortage of human organs, pigs transgenic for human complement control proteins are being considered as potential donors. such xenotransplantation raises concerns about the potential zoonotic transmission of viruses including porcine cytomegalovirus (pcmv), an endemic infection of pigs. similar to hcmv and pcmv tra ... | 2003 | 12588647 |
| approaching virus safety in xenotransplantation: a search for unrecognized herpesviruses in pigs. | the identification of porcine viruses so far unrecognized is required to minimize virus-related risks associated with xenotransplantation. we used a pan-herpes consensus polymerase chain reaction assay to search for unrecognized porcine species of the herpesviridae. the assay targets conserved regions of the herpesvirus dna polymerase (dpol) gene, using primers that were modified to diminish the assay's recognition capacity for the highly prevalent porcine lymphotropic herpesviruses 1, 2 and 3 ( ... | 2003 | 12795684 |
| porcine cytomegalovirus and coagulopathy in pig-to-primate xenotransplantation. | a rapidly progressive disorder termed consumptive coagulopathy (cc) has been observed frequently in pig-to-baboon renal xenotransplantation. cc may be initiated by endothelial activation and induction of procoagulant factors after immunologic injury or infection, or by molecular incompatibilities between porcine coagulation proteins and primate clotting factors. the activation of porcine (p) cytomegalovirus (pcmv) and baboon (b) cmv infections has been documented in pig-to-primate xenotransplant ... | 2003 | 12811243 |
| in situ hybridization and immunohistochemistry for the detection of porcine cytomegalovirus. | to establish in situ hybridization and immunohistochemistry based-assays for the detection of porcine cytomegalovirus, routinely processed renal tissue sections from 34 diseased piglets suspected of having the infection were obtained and examined. using hematoxylin and eosin, porcine cytomegalovirus inclusion bodies were found in the nucleus of renal epithelial cells and capillary endothelial cells in the renal medulla in 30 cases. inclusion bodies corresponding to porcine cytomegalovirus mrna a ... | 2012 | 22008295 |
| glycoprotein b gene-based phylogenetic analysis of porcine cytomegalovirus isolates. | | 2011 | 22149503 |
| Seroprevalence of porcine cytomegalovirus and sapovirus infection in pigs in Hunan province, China. | The seroprevalence of porcine cytomegalovirus (PCMV) and sapovirus (SaV) infections in pigs was investigated in Hunan province, China, between May 2005 and October 2010. A total of 500 pig serum samples collected from 10 representative administrative regions in Hunan province were evaluated for antibodies against PCMV and SaV using enzyme-linked immunosorbent assay (ELISA). The overall seroprevalence of porcine cytomegalovirus and sapovirus in pigs was 96.40% (482/500) and 63.40% (317/500), and ... | 2011 | 22167251 |
| diagnostic investigation of porcine periweaning failure-to-thrive syndrome: lack of compelling evidence linking to common porcine pathogens. | porcine periweaning failure-to-thrive syndrome (pfts), an increasingly recognized syndrome in the swine industry of north america, is characterized by the anorexia of nursery pigs noticeable within 1 week of weaning, and progressive loss of body condition and lethargy during the next 1-2 weeks. morbidity caused by pfts is moderate, but case fatality is high. the etiology of pfts is presently unknown and may include infectious agent(s), noninfectious factors, or both. pfts was identified in a hig ... | 2011 | 22362939 |
| development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions. | porcine cytomegalovirus (pcmv) induces silent infection in adult pigs but more frequently causes fatal, generalized infection in newborn piglets. this study aimed to develop a new loop-mediated isothermal amplification (lamp) method for the sensitive, rapid, and inexpensive detection of pcmv under field conditions. | 2012 | 23272902 |
| xenotransplantation of solid organs in the pig-to-primate model. | xenotransplantation using pig organs could solve the significant increasing shortage of donor organs for allotransplantation. in the last two decades, major progress has been made in understanding the xenoimmunobiology of pig-to-nonhuman primate transplantation, and today we are close to clinical trials. the ability to genetically engineer pigs, such as human decay-accelerating factor (hdaf), cd46 (membrane cofactor protein), or alpha1,3-galactosyltransferase gene-knockout (gt-ko), has been a si ... | 2009 | 18955143 |
| an investigation of the pathology and pathogens associated with porcine respiratory disease complex in denmark. | respiratory infections are among the most important diseases of growing pigs. in order to elucidate the multifactorial aetiology of porcine respiratory disease complex (prdc) in denmark, lungs from 148 finishing pigs with cranioventral bronchopneumonia (case group) and 60 pigs without lung lesions (control group) were collected from abattoirs. the pathogens involved in prdc and their interactions were identified and linked to the histopathological diagnosis. the lung samples were cultured for ba ... | 2010 | 20181357 |
| microbiological safety of porcine islets: comparison with source pig. | pig islet donors intended for clinical xenotransplantation for the treatment of diabetes must meet stringent conditions. among others, viruses with the potential to cross the species barrier should be excluded from the herd: this list includes encephalomyocarditis virus (emcv), hepatitis e virus (hev), porcine cytomegalovirus (pcmv) and porcine γ-lymphotropic herpesvirus (plhv). as an islet product is isolated from the pancreas and then subjected to culture before implantation, the question is r ... | 2011 | 21496116 |