| transmission of lactobacillus jensenii and lactobacillus acidophilus from mother to child at time of delivery. | the presence of lactobacillus jensenii and lactobacillus acidophilus has been studied in specimens from the rectum and vagina of the mother, from the mouth of the infant at the time of delivery, and from the mouth and rectum of infants six days of age. l. jensenii could be differentiated from other species of lactobacilli by the following combination of characteristics: production of only d-lactate, hydrolysis of arginine, and fermentation of cellobiose, galactose, and ribose, but not of lactose ... | 1975 | 809467 |
| hydrogen peroxide production by lactobacillus species: correlation with susceptibility to the spermicidal compound nonoxynol-9. | facultative anaerobic lactobacilli were recovered from the vaginas of 96.8% of 63 nonpregnant, healthy, premenopausal women. the predominant species were lactobacillus jensenii, lactobacillus acidophilus, and lactobacillus casei. of the women, 74.6% had hydrogen peroxide-producing lactobacilli, 22.2% had non-hydrogen peroxide-producing lactobacilli, and 3.2% had no lactobacilli. none of the 68 isolates had catalase activity. some 68.2% of the isolates were inhibited by concentrations of less tha ... | 1992 | 1316413 |
| could bacterial vaginosis be due to the competitive suppression of lactobacilli by aerobic microorganisms? | bacterial vaginosis (bv) is characterized by lack of lactobacilli and predominance of anaerobic species. ciprofloxacin is active against a great number of aerobic bacteria. the effect of ciprofloxacin on the vaginal microbial flora was studied both in healthy subjects and in patients with bv in order to test if bv may be due to competitive suppression of lactobacilli by aerobic species. about half of the patients were relieved of their symptoms. no adverse effects on the microbial profile or col ... | 1992 | 1559624 |
| lactobacillus jensenii prosthetic valve endocarditis. | | 1990 | 2273281 |
| nucleic acids of species of lactobacillus. | nucleic acid composition of 17 cultures representing the type or neotype strains of 15 named lactobacillus species was studied. nucleic acid characterization of these isolates was accompanied by a comparative study of conventional phenotypic reactions. the overall guanine plus cytosine mean deoxyribonucleic acid base composition ranged from 33 to 50% and genome sizes varied between 700 and 1500 x 10(6) daltons. sporolactobacillus inulinus contained 2500 x 10(6) daltons of chromosomal deoxyribonu ... | 1985 | 3939582 |
| lactobacillus jensenii sp.nov., a new representative of the subgenus thermobacterium. | | 1970 | 5493596 |
| identification and plasmid profiles of lactobacillus species from the vagina of 100 healthy women. | it was hypothesized that lactobacillus acidophilus might not be the dominant aerobic or microaerophilic lactobacillus species in the human vagina, and that the dominant isolates are likely to express a variety of properties associated with colonization. to examine the question, vaginal swabs were collected from 100 healthy premenopausal women and cultured to detect the dominant aerobic or microaerophilic isolates of lactobacillus. only eight species were detected, with lactobacillus jensenii bei ... | 1996 | 8871112 |
| adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. | three strains of lactobacillus, identified as lactobacillus acidophilus, lactobacillus gasseri, and lactobacillus jensenii, were selected from among 70 isolates from the vaginas of healthy premenopausal women for properties relevant to mucosal colonization or antagonism. all three self-aggregated and adhered to epithelial vaginal cells, displacing well-known vaginal pathogens, such as g. vaginalis, and inhibiting the growth in vitro of escherichia coli and streptococcus agalactiae. the surface c ... | 1998 | 9573080 |
| differentiation of lactobacillus species by molecular typing. | a total of 64 type, reference, clinical, health food, and stock isolates of microaerophilic lactobacillus species were examined by restriction fragment length polymorphisms. of particular interest were members of six of the eight species most commonly recovered from the vaginas of healthy premenopausal women, namely, lactobacillus jensenii, l. casei, l. rhamnosus, l. acidophilus, l. plantarum, and l. fermentum. six main groupings were identified on the basis of ribotyping. this technique was abl ... | 1998 | 9647809 |
| lactobacillus fornicalis sp. nov., isolated from the posterior fornix of the human vagina. | twelve strains isolated from the posterior fornix fluid of the human vagina were identified as lactobacillus johnsonii, lactobacillus acidophilus, lactobacillus gallinarum and lactobacillus crispatus based on numerical analyses of total soluble cell protein profiles and randomly amplified polymorphic dna (rapd)-pcr banding patterns. five strains grouped with the type strains of lactobacillus gasseri (dsm 20077t) and lactobacillus jensenii (dsm 20557t) at r > or = 0.83 in one protein profile clus ... | 2000 | 10843070 |
| rapid identification of 11 human intestinal lactobacillus species by multiplex pcr assays using group- and species-specific primers derived from the 16s-23s rrna intergenic spacer region and its flanking 23s rrna. | rapid and reliable two-step multiplex polymerase chain reaction (pcr) assays were established to identify human intestinal lactobacilli; a multiplex pcr was used for grouping of lactobacilli with a mixture of group-specific primers followed by four multiplex pcr assays with four sorts of species-specific primer mixtures for identification at the species level. primers used were designed from nucleotide sequences of the 16s-23s rrna intergenic spacer region and its flanking 23s rrna gene of membe ... | 2000 | 10856652 |
| vaginal lactobacillus flora of healthy swedish women. | species of the lactobacillus acidophilus complex are generally considered to constitute most of the vaginal lactobacillus flora, but the flora varies between studies. however, this may be due to difficulties in identifying the closely related species within the l. acidophilus complex by using traditional methods and to variations in the vaginal status of the participants. two hundred two isolates from the vaginal fluids of 23 swedish women without bacterial vaginosis, as defined by the criteria ... | 2002 | 12149323 |
| antimicrobial components of vaginal fluid. | we examined the antimicrobial activity and composition of vaginal fluid. | 2002 | 12237628 |
| inhibition of neisseria gonorrhoeae by lactobacillus species that are commonly isolated from the female genital tract. | epidemiological studies suggest h(2)o(2)-producing lactobacilli protect women against gonorrhea. here we demonstrate that lactobacillus crispatus and lactobacillus jensenii, the most common lactobacilli in the female genital tract, inhibit gonococci in both acidic and neutral ph conditions. inhibition was neutralized by bovine catalase, suggesting that h(2)o(2) is the primary mediator of inhibition. | 2002 | 12438404 |
| identification of randomly selected colonies of lactobacilli from normal vaginal fluid by pyrosequencing of the 16s rdna variable v1 and v3 regions. | the present study aimed to characterize lactobacilli in vaginal fluid from 23 adult healthy women by using high-throughput dna sequencing for identification of a large number of randomly selected colonies appearing on rogosa and blood agar. the typing method was based on broad-range pcr of 16s rrna gene variable regions v1 and v3, pyrosequencing, and classification of the fragments by alignment with ncbi-catalogued sequences and type strain sequences. four major groups of sequences were found am ... | 2002 | 12588421 |
| antimicrobial properties of self-etching primer-bonding systems. | self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ... | 2003 | 12670069 |
| inhibition of hiv infectivity by a natural human isolate of lactobacillus jensenii engineered to express functional two-domain cd4. | the predominant mode of hiv transmission worldwide is via heterosexual contact, with the cervico-vaginal mucosa being the main portal of entry in women. the cervico-vaginal mucosa is naturally colonized with commensal bacteria, primarily lactobacilli. to address the urgent need for female-controlled approaches to block the heterosexual transmission of hiv, we have engineered natural human vaginal isolates of lactobacillus jensenii to secrete two-domain cd4 (2d cd4) proteins. the secreted 2d cd4 ... | 2003 | 12972635 |
| managing embolic myocardial infarction in infective endocarditis: current options. | systemic embolization is common in infective endocarditis and is known to occur in 45-65% of cases. coronary artery embolism has been seen in as many as 60% of cases at necropsy. however, it only rarely has been described as resulting in transmural myocardial infarction. in most cases, coronary embolism is inferred from circumstantial evidence. we present two patients with myocardial infarction in the setting of acute infective endocarditis. current issues regarding the management of myocardial ... | 2004 | 16230184 |
| lactobacillus bacteremia, species identification, and antimicrobial susceptibility of 85 blood isolates. | data regarding antimicrobial susceptibility of clinical lactobacillus isolates are scarce, and appropriate interpretation criteria for susceptibility tests are not available. | 2006 | 16447101 |
| vaginal lactobacillus isolates inhibit uropathogenic escherichia coli. | the purpose of this study was to investigate the antibacterial activities of lactobacillus jensenii ks119.1 and ks121.1, and lactobacillus gasserii ks120.1 and ks124.3 strains isolated from the vaginal microflora of healthy women, against uropathogenic, diffusely adhering afa/dr escherichia coli (afa/dr daec) strains ih11128 and 7372 involved in recurrent cystitis. we observed that some of the lactobacillus isolates inhibited the growth and decreased the viability of e. coli ih11128 and 7372. in ... | 2006 | 16553843 |
| selection and identification of anaerobic lactobacilli producing inhibitory compounds against vaginal pathogens. | two strains of lactobacillus crispatus (15l08 and 21l07) and one strain of lactobacillus jensenii (5l08) were selected from amongst 100 isolates from the vaginas of healthy premenopausal women for properties relevant to mucosal colonization and the production of h2o2 and/or bacteriocin-like compound. all three strains self-aggregated and adhered to vaginal epithelial cells, displacing well-known vaginal pathogens, such as gardnerella vaginalis and candida albicans. lactobacillus crispatus 15l08 ... | 2006 | 16965354 |
| reversible deficiency of antimicrobial polypeptides in bacterial vaginosis. | bacterial vaginosis is a common condition associated with increased risk of sexually transmitted diseases, including human immunodeficiency virus infections. in contrast, vulvovaginal candidiasis has a much weaker association with sexually transmitted diseases. we found that vaginal lavage fluid from women with bacterial vaginosis is deficient in antimicrobial polypeptides and antimicrobial activity compared to fluid from healthy women or women with vulvovaginal candidiasis. effective treatment ... | 2006 | 16988245 |
| engineered vaginal lactobacillus strain for mucosal delivery of the human immunodeficiency virus inhibitor cyanovirin-n. | women are at significant risk of human immunodeficiency virus (hiv) infection, with the cervicovaginal mucosa serving as a major portal for virus entry. female-initiated preventatives, including topical microbicides, are urgently needed to help curtail the hiv/aids pandemic. here we report on the development of a novel, live microbicide that employs a natural vaginal strain of lactobacillus jensenii engineered to deliver the potent hiv inhibitor cyanovirin-n (cv-n). to facilitate efficient expre ... | 2006 | 17005802 |
| lactobacillus strains isolated from the vaginal microbiota of healthy women inhibit prevotella bivia and gardnerella vaginalis in coculture and cell culture. | the purpose of this study was to investigate how human vaginal isolates of lactobacillus acidophilus, lactobacillus jensenii, lactobacillus gasseri and lactobacillus crispatus inhibit the vaginosis-associated pathogens gardnerella vaginalis and prevotella bivia. results show that all the strains in coculture condition reduced the viability of g. vaginalis and p. bivia, but with differing degrees of efficacy. the treatment of g. vaginalis- and p. bivia-infected cultured human cervix epithelial he ... | 2006 | 17059467 |
| development of antioxidant activity in milk whey during fermentation with lactic acid bacteria. | to investigate the production of antioxidant activity during fermentation with commonly used dairy starter cultures. moreover, to study the development of antioxidant activity during fermentation, and the connection to proteolysis and bacterial growth. | 2007 | 17184325 |
| [septicaemia due to lactobacillus jensenii: bacteriological diagnostic orientation]. | septicaemia concerning lactobacillus jensenii is exceptional. this bacteria commensal of the normal human flora is known for its low pathogenicity. we report here the observation of a woman, 50 years old, who was admitted in our service for an obstructive acute renal failure and who has presented a septicaemia due to l. jensenii, with a favourable issue by use of antibiotics (amoxicillin-clavulanic acid). this observation permits to report the clinical and bacteriological characteristics of l. j ... | 2007 | 17502304 |
| vaginal lactobacillus microbiota of healthy women in the late first trimester of pregnancy. | this study was undertaken to characterise the dominant species of lactobacillus colonising the vagina of healthy pregnant women, to examine some of their phenotypic and genotypic properties, and to gain a better understanding of the potential role of species, which might be associated with infection-free status. | 2007 | 17877778 |
| inhibition of neisseria gonorrhoeae epithelial cell interactions by vaginal lactobacillus species. | high levels of lactobacillus, the dominant genus of the healthy human vaginal microbiota, have been epidemiologically linked to a reduced risk of infection following exposure to the sexually transmitted pathogen neisseria gonorrhoeae. in this work, a cell culture model of gonococcal infection was adapted to examine the effects of lactobacilli on gonococcal interactions with endometrial epithelial cells in vitro. precolonization of epithelial cells with lactobacillus jensenii, lactobacillus gasse ... | 2008 | 18411284 |
| gardnerella vaginalis and lactobacillus sp in liquid-based cervical samples in healthy and disturbed vaginal flora using cultivation-independent methods. | our objective was to determine the morphotype of the adherent bacteria in liquid-based cytology (lbc) in smears with healthy and disturbed vaginal flora. and to use pcr technology on the same fixed cell sample to establish dna patterns of the 16s rna genes of the bacteria in the sample. thirty samples were randomly selected from a large group of cervical cell samples suspended in a commercial coagulant fixative "(boonfix)." pcr was used to amplify dna of five bacterial species: lactobacillus aci ... | 2008 | 18418885 |
| bacteremia and pyelonephritis caused by lactobacillus jensenii in a patient with urolithiasis. | | 2008 | 18432039 |
| endocarditis due to lactobacillus jensenii in a salvin's amazon parrot (amazona autumnalis salvini). | a 30-year-old salvin's amazon parrot (amazona autumnalis salvini) with a history of a lifelong poor diet and inappropriate housing was presented in lateral recumbency to a veterinary teaching hospital for further evaluation. radiological and ultrasonographic examination revealed a mild proventricular dilatation, mild hepatomegaly, signs of enteritis and airsacculitis. the main laboratory findings included a mild macrocytic hyperchromic anaemia, hypoglobulinaemia, decreased bile acids and increas ... | 2009 | 19156580 |
| the human vaginal bacterial biota and bacterial vaginosis. | the bacterial biota of the human vagina can have a profound impact on the health of women and their neonates. changes in the vaginal microbiota have been associated with several adverse health outcomes including premature birth, pelvic inflammatory disease, and acquisition of hiv infection. cultivation-independent molecular methods have provided new insights regarding bacterial diversity in this important niche, particularly in women with the common condition bacterial vaginosis (bv). pcr method ... | 2009 | 19282975 |
| a chinese rhesus macaque (macaca mulatta) model for vaginal lactobacillus colonization and live microbicide development. | we sought to establish a nonhuman primate model of vaginal lactobacillus colonization suitable for evaluating live microbial microbicide candidates. | 2009 | 19367737 |
| the superior folding of a rantes analogue expressed in lactobacilli as compared to mammalian cells reveals a promising system to screen new rantes mutants. | development of effective topical microbicides for the prevention of hiv-1 sexual transmission represents a primary goal for the control of the aids pandemic. the viral coreceptor ccr5, used by the vast majority of primary hiv-1 isolates, is considered a primary target molecule. rantes and its derivatives are the most suitable protein-based compounds to fight hiv-1 via ccr5 targeting. yet, receptor activation should be avoided to prevent pro-inflammatory effects and possibly provide anti-inflamma ... | 2009 | 19573605 |
| lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse. | we previously isolated five strains of putative lactobacilli from the faeces of a thoroughbred horse (a 4-year-old male). of the five strains, four were identified as members of existing lactobacillus species; however, sequence analysis of the 16s rrna gene revealed that the fifth isolate, di70(t), showed approximately 97 % identity (1325/1366 bp) with the type strain of lactobacillus delbrueckii. therefore, we considered the possibility that di70(t) represents a novel species of the genus lacto ... | 2010 | 19648335 |
| immunomodulation in gut-associated lymphoid tissue of neonatal chicks by immunobiotic diets. | developmental changes in immunocompetent cells of the gut during the first week posthatch were determined in broiler chicks fed immunobiotic lactic acid bacteria in the form of lactobacillus jensenii tl2937-, lactobacillus gasseri jcm1131(t)-, lactobacillus delbrueckii ssp. bulgaricus niaib6-, or l. gasseri tl2919-supplemented diets. the relative weights of spleen and bursa of fabricius in chicks fed the immunobiotic diets were slightly higher than the control valued at 1 and 3 d of age, with th ... | 2009 | 19903951 |
| the vaginal bacterial communities of japanese women resemble those of women in other racial groups. | to determine whether different racial groups shared common types of vaginal microbiota, we characterized the composition and structure of vaginal bacterial communities in asymptomatic and apparently healthy japanese women in tokyo, japan, and compared them with those of white and black women from north america. the composition of vaginal communities was compared based on community profiles of terminal restriction fragments of 16s rrna genes and phylogenetic analysis of cloned 16s rrna gene seque ... | 2010 | 19912342 |
| in vitro inhibitory activity of human vaginal lactobacilli against pathogenic bacteria associated with bacterial vaginosis in kenyan women. | lactobacilli have been shown to inhibit in vitro growth of many pathogens and have been used as probiotics to treat a broad range of gastrointestinal and/or vaginal disorders. we sought to determine the in vitro inhibitory potential of lactobacilli of vaginal origin to some bacteria associated with bacterial vaginosis (bv), to characterize the inhibitory substances produced by these lactobacilli and to assess h2o2 production. vaginal specimens were obtained by swabbing the lateral vaginal walls ... | 2010 | 19925874 |
| biosynthesis and degradation of h2o2 by vaginal lactobacilli. | hydrogen peroxide production by vaginal lactobacilli represents one of the most important defense mechanisms against vaginal colonization by undesirable microorganisms. to quantify the ability of a collection of 45 vaginal lactobacillus strains to generate h(2)o(2), we first compared three published colorimetric methods. it was found that the use of da-64 as a substrate rendered the highest sensitivity, while tetramethyl-benzidine (tmb) maintained its linearity from nanomolar to millimolar h(2)o ... | 2010 | 19948869 |
| endocarditis caused by lactobacillus jensenii in an immunocompetent patient. | lactobacilli are gram-positive rod-shaped bacteria that inhabit the oral cavity, gastrointestinal tract, vagina and nasal cavity. in this report, a rare case of lactobacillus jensenii endocarditis in a 47-year-old immunocompetent patient is described. blood cultures and a replaced mitral valve were positive for l. jensenii as assessed by 16s rrna gene sequencing. based on susceptibility tests the patient was successfully treated with a mixture of teicoplanin and meropenem antimicrobial therapy. | 2010 | 20133416 |
| quantitative pcr assessments of bacterial species in women with and without bacterial vaginosis. | knowledge of the abundance of bacterial species in vaginal communities will help us to better understand their role in health and disease. however, progress in this field has been limited because quantifying bacteria in natural specimens is an arduous process. we developed quantitative real-time pcr (qpcr) assays to facilitate assessments of bacterial abundance in vaginal specimens and evaluated the utility of these assays by measuring species abundance in patients whose vaginal floras were clin ... | 2010 | 20305015 |
| lactobacillus jensenii surface-associated proteins inhibit neisseria gonorrhoeae adherence to epithelial cells. | high numbers of lactobacilli in the vaginal tract have been correlated with a decreased risk of infection by the sexually transmitted pathogen neisseria gonorrhoeae. we have previously shown that lactobacillus jensenii, one of the most prevalent microorganisms in the healthy human vaginal tract, can inhibit gonococcal adherence to epithelial cells in culture. here we examined the role of the epithelial cells and the components of l. jensenii involved in the inhibition of gonococcal adherence. l. ... | 2010 | 20385752 |
| identification of the bacterial biodiversity in koumiss by denaturing gradient gel electrophoresis and species-specific polymerase chain reaction. | bacterial biodiversity in traditional koumiss fermented milk was studied by denaturing gradient gel electrophoresis (dgge). target dna bands were identified according to the reference species ladder, constructed in this study. comigrating bands present in the dgge profiles were resolved by species-specific pcr. the results revealed a novel bacterial profile and extensive bacterial biodiversity in koumiss. the dominant lactic acid bacteria included lactobacillus acidophilus, lactobacillus helveti ... | 2010 | 20412906 |
| temporal variability of human vaginal bacteria and relationship with bacterial vaginosis. | little is known about short-term bacterial fluctuations in the human vagina. this study used pcr to assess the variability in concentrations of key vaginal bacteria in healthy women and the immediate response to antibiotic treatment in women with bacterial vaginosis (bv). | 2010 | 20419168 |
| engineering of lactobacillus jensenii to secrete rantes and a ccr5 antagonist analogue as live hiv-1 blockers. | the development of effective microbicides for the prevention of hiv-1 sexual transmission represents a primary goal for the control of aids epidemics worldwide. a promising strategy is the use of bacteria belonging to the vaginal microbiota as live microbicides for the topical production of hiv-1 inhibitors. we have engineered a human vaginal isolate of lactobacillus jensenii to secrete the anti-hiv-1 chemokine rantes, as well as c1c5 rantes, a mutated analogue that acts as a ccr5 antagonist and ... | 2010 | 20479208 |
| candidate vaginal microbicides with activity against chlamydia trachomatis and neisseriagonorrhoeae. | vaginal microbicides with activity towards organisms that cause sexually transmitted infections have been proposed as a strategy to reduce transmission. small-molecule inhibitors of chlamydia trachomatis serovar d belonging to the class of salicylidene acylhydrazides (inps) have been shown to work through a mechanism that involves iron restriction. expanding on this work, ten inps were tested against a lymphogranuloma venereum strain of c. trachomatis (serovar l2), neisseria gonorrhoeae, and hyd ... | 2010 | 20605703 |
| expression, purification, crystallization and preliminary x-ray studies of lactobacillus jensenii enolase. | recombinant lactobacillus jensenii enolase fused to a c-terminal noncleavable his tag was expressed in escherichia coli, purified and crystallized by sitting-drop vapor diffusion. a complete data set was collected to 3.25 a resolution. the crystals belonged to space group i4, with unit-cell parameters a=b=145.31, c=99.79 a. there were two protein subunits in the asymmetric unit, which gave a matthews coefficient vm of 2.8 a3 da(-1), corresponding to 55.2% solvent content. | 2010 | 20693674 |
| induction, structural characterization, and genome sequence of lv1, a prophage from a human vaginal lactobacillus jensenii strain. | the prophage lv1, harbored by a vaginal lactobacillus jensenii isolate, was induced by several different anticancer, antimicrobial, and antiseptic agents, suggesting that they contribute to the adverse vaginal effects associated with their therapeutic use. of special interest with respect to its novelty was the inducing effect of nonoxynol-9, a non-ionic detergent commonly used as a spermicide. the lv1 genome consists of a 38,934-bp dsdna molecule with cohesive ends, in which 48 orfs were recogn ... | 2010 | 20890845 |
| identification of predominant culturable vaginal lactobacillus species and associated bacteriophages from women with and without vaginal discharge syndrome in south africa. | lactobacillus jensenii, lactobacillus crispatus, lactobacillus iners, lactobacillus gasseri and lactobacillus vaginalis were identified by 16s rrna gene sequencing as the predominant culturable vaginal lactobacillus species in a group of south african women, comprising 24, 22, 10, 10 and 9 %, respectively. a significant effect of vaginal discharge syndrome (vds) and bacterial vaginosis (bv) on the distribution of predominant lactobacillus species was observed. whilst l. crispatus isolates were a ... | 2010 | 21030503 |
| (gtg)(5)-pcr fingerprinting of lactobacilli isolated from cervix of healthy women. | a group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (gtg)(5)-polymerase chain reaction (pcr) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. obtained fingerprints were compared with profiles available in an in-house database of the ccm bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. selected strains representing individual clusters were further identi ... | 2011 | 21390569 |
| anaerobic bacteria colonizing the lower airways in lung cancer patients. | anaerobes comprise most of the endogenous oropharyngeal microflora, and can cause infections of airways in lung cancer patients who are at high risk for respiratory tract infections. the aim of this study was to determine the frequency and species diversity of anaerobes in specimens from the lower airways of lung cancer patients. sensitivity of the isolates to conventional antimicrobial agents used in anaerobe therapy was assessed. respiratory secretions obtained by bronchoscopy from 30 lung can ... | 2011 | 21744326 |
| prevention of vaginal shiv transmission in macaques by a live recombinant lactobacillus. | most human immunodeficiency virus (hiv) transmissions in women occur through the cervicovaginal mucosa, which is coated by a bacterial biofilm including lactobacillus. this commensal bacterium has a role in maintaining a healthy mucosa and can be genetically engineered to produce antiviral peptides. here, we report a 63% reduction in transmission of a chimeric simian/hiv (shiv(sf162p3)) after repeated vaginal challenges of macaques treated with lactobacillus jensenii expressing the hiv-1 entry i ... | 2011 | 21734653 |
| Demonstration of vaginal colonization with GusA-expressing Lactobacillus jensenii following oral delivery in rhesus macaques. | The vaginal microbiome, which harbors beneficial Lactobacillus strains, is believed to be a major host defense mechanism for preventing infections of the urogenital tract. It has been suggested that the gastrointestinal tract serves as a reservoir for lactobacilli that colonize the vagina. Using rhesus macaques, we examined whether oral delivery of human vaginal Lactobacillus jensenii 1153-1646, a GusA-producing strain, would result in colonization of the rectum and the vagina. Lactobacilli were ... | 2011 | 21907793 |
| [selection and genotyping of lactobacillus with potential preventive effect by repetitive element sequence-based pcr analysis]. | we selected and characterized isolates of lactobacillus crispatus (l. crispatus) for potential preventing infections of the female reproductive tract. we cultured vaginal swabs from healthy volunteers on de man, rogosa and sharpe (mrs) agar and identified the isolates at the species level by 16s rrna sequence and genotyped the isolates of lactobacillus by pcr amplification of repetitive bacterial dna elements (rep-pcr). furthermore, 10 l. crispatus strains were assessed for hydrogen peroxide (h2 ... | 2011 | 21845850 |
| Lactobacillus species shift in distal esophagus of high-fat-diet-fed rats. | To analyze the microbiota shift in the distal esophagus of Sprague-Dawley rats fed a high-fat diet. | 2011 | 21912459 |
| lactobacillus jensenii bacteremia and endocarditis after dilatation and curettage: case report and literature review. | | 2011 | 21866337 |
| antagonistic effect of some starter cultures on enterobacteriaceae (e. coli). | the ability of lactobacillus jensenii and streptococcus 17sb to inbhibit escherichia coli was demonstrated. the practical application of the inhibition was examined. possible roles for several factors in the mechanism of inhibition by lactobacillus jensenii and streptococcus 17sb are briefly discussed. the effects of ph reached and acids produced by starters on the growth of escherichia coli are described. | 1989 | 22056161 |
| Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway. | The effect of Lactobacillus jensenii TL2937 on the inflammatory immune response triggered by enterotoxigenic Escherichia coli (ETEC) and lipopolysaccharide (LPS) in a porcine intestinal epitheliocyte cell line (PIE cells) was evaluated. Challenges with ETEC or LPS elicited Toll-like receptor 4 (TLR4)-mediated inflammatory responses in cultured PIE cells, indicating that our cell line may be useful for studying inflammation in the guts of weaning piglets. In addition, we demonstrated that L. jens ... | 2012 | 22083706 |
| testing of viscous anti-hiv microbicides using lactobacillus. | the development of topical microbicides for intravaginal use to prevent hiv infection requires that the drugs and formulated products be nontoxic to the endogenous vaginal lactobacillus. in 30min exposure tests we found dapivirine, tenofovir and uc781 (reverse transcriptase inhibitor anti-hiv drugs) as pure drugs or formulated as film or gel products were not deleterious to lactobacillus species; however, psc-rantes (a synthetic ccr5 antagonist) killed 2 strains of lactobacillus jensenii. to dem ... | 2011 | 22226641 |
| lactobacillus proteins are associated with the bactericidal activity against e. coli of female genital tract secretions. | female genital tract secretions are bactericidal for escherichia (e.) coli ex vivo. however, the intersubject variability and molecules that contribute to this activity have not been defined. | 2012 | 23185346 |
| phase i randomized safety study of twice daily dosing of acidform vaginal gel: candidate antimicrobial contraceptive. | acidform gel, an acid-buffering product that inactivates spermatozoa, may be an effective topical non-hormonal contraceptive. this study was designed to evaluate the safety of vaginal dosing and effects of acidform on mucosal immune mediators, antimicrobial properties of genital secretions, and vaginal microbiota. | 2012 | 23056520 |
| altered biomarkers of mucosal immunity and reduced vaginal lactobacillus concentrations in sexually active female adolescents. | genital secretions collected from adult women exhibit in vitro activity against herpes simplex virus (hsv) and escherichia coli (e. coli), but prior studies have not investigated this endogenous antimicrobial activity or its mediators in adolescent females. | 2012 | 22808157 |
| extravaginal reservoirs of vaginal bacteria as risk factors for incident bacterial vaginosis. | bacterial vaginosis (bv) represents shifts in microbiota from lactobacillus spp. to diverse anaerobes. although antibiotics relieve symptoms and temporarily eradicate bv-associated bacteria (bvab), bv usually recurs. we investigated the role of extravaginal bvab reservoirs in recurrence. | 2012 | 22448002 |
| relationship of specific bacteria in the cervical and vaginal microbiotas with cervicitis. | cervicitis is an inflammatory condition of the cervix associated with upper genital tract infection and reproductive complications. although cervicitis can be caused by several known pathogens, the etiology frequently remains obscure. here we investigate vaginal bacteria associated with bacterial vaginosis as potential causes of cervicitis. | 2015 | 26267872 |
| metabolic signatures of bacterial vaginosis. | bacterial vaginosis (bv) is characterized by shifts in the vaginal microbiota from lactobacillus dominant to a microbiota with diverse anaerobic bacteria. few studies have linked specific metabolites with bacteria found in the human vagina. here, we report dramatic differences in metabolite compositions and concentrations associated with bv using a global metabolomics approach. we further validated important metabolites using samples from a second cohort of women and a different platform to meas ... | 2015 | 25873373 |
| lactobacillus species as biomarkers and agents that can promote various aspects of vaginal health. | the human body is colonized by a vast number of microorganisms collectively referred to as the human microbiota. one of the main microbiota body sites is the female genital tract, commonly dominated by lactobacillus spp., in approximately 70% of women. each individual species can constitute approximately 99% of the ribotypes observed in any individual woman. the most frequently isolated species are lactobacillus crispatus, lactobacillus gasseri, lactobacillus jensenii and lactobacillus iners. re ... | 2015 | 25859220 |
| antimicrobial and antibiofilm potential of biosurfactants isolated from lactobacilli against multi-drug-resistant pathogens. | biosurfactants (bs) are amphiphilic compounds produced by microbes, either on the cell surface or secreted extracellularly. bs exhibit strong antimicrobial and anti-adhesive properties, making them good candidates for applications used to combat infections. in this study, our goal was to assess the in vitro antimicrobial, anti-adhesive and anti-biofilm abilities of bs produced by lactobacillus jensenii and lactobacillus rhamnosus against clinical multidrug resistant (mdr) strains of acinetobacte ... | 2014 | 25124936 |
| cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity. | persistent infection with oncogenic human papillomavirus (hpv) is necessary for cervical carcinogenesis. although evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of hpv infections, this has yet to be described in women with cervical intra-epithelial neoplasia (cin). we hypothesised that increasing microbiome diversity is associated with increasing cin severity. llumina miseq sequencing of 16s rrna gene amplicons was used to characterise the ... | 2015 | 26574055 |
| identification of vaginal fluid, saliva, and feces using microbial signatures in a han chinese population. | in recent years, forensic scientists have focused on the discrimination of body fluids using microbial signatures. in this study, we performed pcr-based detection of microbial signatures of vaginal fluid, saliva, and feces in a han chinese population. we investigated the 16s rrna genes of lactobacillus crispatus, lactobacillus gasseri, lactobacillus jensenii, lactobacillus iners, and atopobium vaginae in vaginal fluid, the 16s rrna and the glucosyltransferase enzyme genes of streptococcus saliva ... | 2016 | 27570236 |
| a dna tool for early detection of vaginal dysbiosis in african women. | a next-generation diagnostic tool for bacterial vaginosis, consisting of quantitative and/or qualitative molecular criteria, has not yet been identified. the optimal diagnostic tool should not only diagnose bacterial vaginosis in diverse populations, but should also detect early signs of transition to dysbiosis. we evaluated a tool based on log10-transformed qpcr data for lactobacillus crispatus, lactobacillus iners, lactobacillus jensenii, lactobacillus gasseri, lactobacillus vaginalis, lactoba ... | 2016 | 26577657 |
| establishment of a sensitive system for analysis of human vaginal microbiota on the basis of rrna-targeted reverse transcription-quantitative pcr. | ten specific primer sets, for lactobacillus gasseri, lactobacillus crispatus, atopobium vaginae, gardnerella vaginalis, mobiluncus curtisii, chlamydia trachomatis/muridarum, bifidobacterium longum subsp. longum, bifidobacterium longum subsp. infantis, bifidobacterium adolescentis, and bifidobacterium angulatum, were developed for quantitative analysis of vaginal microbiota. rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) analysis of the vaginal samples from 12 healthy japanese vol ... | 2015 | 25661498 |
| effect of sexual debut on vaginal microbiota in a cohort of young women. | bacterial vaginosis is uncommon in women who are virgins. we estimated effects of sexual debut on vaginal bacterial colonization. | 2012 | 23168754 |
| detection of bacterial 16s ribosomal rna genes for forensic identification of vaginal fluid. | to preliminarily evaluate the applicability of bacterial dna as a marker for the forensic identification of vaginal fluid, we developed and performed pcr-based detection of 16s ribosomal rna genes of lactobacillus spp. dominating the vagina and of bacterial vaginosis-related bacteria from dna extracted from body fluids and stains. as a result, 16s ribosomal rna genes of lactobacillus crispatus, lactobacillus jensenii and atopobium vaginae were specifically detected in vaginal fluid and female ur ... | 2012 | 22316585 |
| characterization of the vaginal microbiota of healthy canadian women through the menstrual cycle. | the vaginal microbial community plays a vital role in maintaining women's health. understanding the precise bacterial composition is challenging because of the diverse and difficult-to-culture nature of many bacterial constituents, necessitating culture-independent methodology. during a natural menstrual cycle, physiological changes could have an impact on bacterial growth, colonization, and community structure. the objective of this study was to assess the stability of the vaginal microbiome of ... | 2014 | 25053998 |
| characterisation of probiotic properties in human vaginal lactobacilli strains. | vaginal lactobacilli offer protection against recurrent urinary infections, bacterial vaginosis, and vaginal candidiasis. | 2016 | 27527701 |
| inhibitory effects of seaweed extracts on the growth of the vaginal bacterium gardnerella vaginalis. | of 44 species of seaweed screened for potential anti-gardnerella vaginalis activity, 27 (61.4%) showed antimicrobial activity by the agar disk-diffusion method. among them, the strongest activities against the pathogen were exhibited by chlorophyta, with ulva pertusa producing an 11.3-mm zone of inhibition at 5 mg disk⁻¹. the mic values of u. pertusa extracts against both g. vaginalis kctc 5096 and kctc 5097, the main cause of vaginosis, were 312 μg ml⁻¹, while the mic values against both candid ... | 2014 | 24813010 |
| modulation of porcine intestinal epitheliocytes immunetranscriptome response by lactobacillus jensenii tl2937. | in order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (pie cells). here, transcriptomic studies using pie cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain lactobacillus jensenii tl2937 against intestinal inflammatory damage in pigs. in additio ... | 2016 | 27824278 |
| development of phage lysin lysa2 for use in improved purity assays for live biotherapeutic products. | live biotherapeutic products (lbps), commonly referred to as probiotics, are typically preparations of live bacteria, such as lactobacillus and bifidobacterium species that are considered normal human commensals. popular interest in probiotics has been increasing with general health benefits being attributed to their consumption, but there is also growing interest in evaluating such products for treatment of specific diseases. while over-the-counter probiotics are generally viewed as very safe, ... | 2015 | 26694451 |
| changes in vaginal microbiota and immune mediators in hiv-1-seronegative kenyan women initiating depot medroxyprogesterone acetate. | depot medroxyprogesterone acetate (dmpa) is associated with hiv acquisition. we studied changes in vaginal microbiota and inflammatory milieu after dmpa initiation. | 2016 | 26914908 |
| characteristics of lactobacillus and gardnerella vaginalis from women with or without bacterial vaginosis and their relationships in gnotobiotic mice. | the objectives of the present study were to evaluate in vitro the production of antagonistic compounds against gardnerella vaginalis by lactobacillus strains isolated from women with or without bacterial vaginosis (bv), and to select one of the better lactobacillus producers of such a substance to be tested in vivo using a gnotobiotic animal model challenged with one of the more sensitive g. vaginalis isolates. a total of 24 isolates from women with and without bv were identified as g. vaginalis ... | 2012 | 22539000 |
| the cervicovaginal microbiota and its associations with human papillomavirus detection in hiv-infected and hiv-uninfected women. | bacterial vaginosis (bv) is characterized by low abundance of lactobacillus species, high ph, and immune cell infiltration and has been associated with an increased risk of human papillomavirus (hpv) infection. we molecularly assessed the cervicovaginal microbiota over time in human immunodeficiency virus (hiv)-infected and hiv-uninfected women to more comprehensively study the hpv-microbiota relationship, controlling for immune status. | 2016 | 27521363 |
| expression of human immunodeficiency virus type 1 neutralizing antibody fragments using human vaginal lactobacillus. | eradication of human immunodeficiency virus type 1 (hiv-1) by vaccination with epitopes that produce broadly neutralizing antibodies is the ultimate goal for hiv prevention. however, generating appropriate immune responses has proven difficult. expression of broadly neutralizing antibodies by vaginal colonizing lactobacilli provides an approach to passively target these antibodies to the mucosa. we tested the feasibility of expressing single-chain and single-domain antibodies (dabs) in lactobaci ... | 2017 | 26950606 |
| in vivo evaluation of safety and toxicity of a lactobacillus jensenii producing modified cyanovirin-n in a rhesus macaque vaginal challenge model. | sexual transmission of human immunodeficiency virus type 1 (hiv-1) across the cervicovaginal mucosa in women is influenced by many factors including the microbiota and the presence of underlying inflammation. it is important that potential hiv preventative agents do not alter the mucosal environment in a way that enhances hiv acquisition. we examined the impact of a "live" microbicide on the vaginal mucosal environment in a rhesus macaque repeated vaginal simian-hiv (shivsf162p3) challenge model ... | 2013 | 24265721 |
| predominant lactobacillus species types of vaginal microbiota in pregnant korean women: quantification of the five lactobacillus species and two anaerobes. | to investigate the predominant lactobacillus species types (lsts) of vaginal microbiota in pregnant korean women by quantifying five lactobacillus species and two anaerobes. | 2016 | 27756178 |
| impact of hydroxychloroquine-loaded polyurethane intravaginal rings on lactobacilli. | the use of polymeric devices for controlled sustained delivery of drugs is a promising approach for the prevention of hiv-1 infection. unfortunately, certain microbicides, when topically applied vaginally, may be cytotoxic to vaginal epithelial cells and the protective microflora present within the female genital tract. in this study, we evaluated the impact of hydroxychloroquine (hcq)-loaded, reservoir-type, polyurethane intravaginal rings (ivrs) on the growth of lactobacillus crispatus and lac ... | 2015 | 26416871 |
| comparison of detection methods for vaginal lactobacilli. | vaginal lactobacilli offer protection against microbiota imbalance and genitourinary tract infections. we compared vaginal lactobacilli in 50 estonian women of child-bearing age applying culture-based methods, quantitative pcr and next-generation sequencing (ngs). the culture-based methods found three different lactobacilli: lactobacillus crispatus, lactobacillus jensenii and lactobacillus gasseri. using ngs revealed the presence of l. crispatus in 76%, lactobacillus iners in 52%, l. jensenii in ... | 2015 | 25869280 |
| a simple identification method for vaginal secretions using relative quantification of lactobacillus dna. | in criminal investigations there are some cases in which identifying the presence of vaginal secretions provides crucial evidence in proving sexual assault. however, there are no methods for definitively identifying vaginal secretions. in the present study, we focused on lactobacillus levels in vaginal secretions and developed a novel identification method for vaginal secretions by relative quantification based on real time pcr. we designed a lactobacillus conserved region primer pair (lcp) by a ... | 2014 | 24905338 |
| association between the vaginal microbiota, menopause status, and signs of vulvovaginal atrophy. | the vaginal microbiota helps protect the female genital tract from disease. we sought to describe the composition of the vaginal microbiota in premenopausal, perimenopausal, and postmenopausal women and to explore the association between the microbiota and vulvovaginal atrophy (vva). | 2014 | 24080849 |
| influence of vaginal bacteria and d- and l-lactic acid isomers on vaginal extracellular matrix metalloproteinase inducer: implications for protection against upper genital tract infections. | we evaluated levels of vaginal extracellular matrix metalloproteinase inducer (emmprin) and matrix metalloproteinase (mmp-8) in vaginal secretions in relation to the composition of vaginal bacterial communities and d- and l-lactic acid levels. the composition of vaginal bacterial communities in 46 women was determined by pyrosequencing the v1 to v3 region of 16s rrna genes. lactobacilli were dominant in 71.3% of the women, followed by gardnerella (17.4%), streptococcus (8.7%), and enterococcus ( ... | 2013 | 23919998 |
| [lactobacillus inhibit adhesion of staphylococcus aureus to hela cells]. | to assess the ability of the previously selected human vaginal isolates of lactobacillus crispatus (l. crispatus) t79-3, t90-1 and lactobacillus jensenii (l. jensenii) t118-3, t231-1 to inhibit the growth of staphylococcus aureus and block their adhesion to hela cells. the inhibitory bioactive substances produced by these lactobacillus were also identified. inhibitory substances interaction tests were carried out by using a streak-diffusion method on agar plates. three types of interaction were ... | 2012 | 23016308 |
| behavioral predictors of colonization with lactobacillus crispatus or lactobacillus jensenii after treatment for bacterial vaginosis: a cohort study. | evaluate predictors of vaginal colonization with lactobacilli after treatment for bacterial vaginosis (bv). | 2012 | 22693410 |
| adhesion forces and coaggregation between vaginal staphylococci and lactobacilli. | urogenital infections are the most common ailments afflicting women. they are treated with dated antimicrobials whose efficacy is diminishing. the process of infection involves pathogen adhesion and displacement of indigenous lactobacillus crispatus and lactobacillus jensenii. an alternative therapeutic approach to antimicrobial therapy is to reestablish lactobacilli in this microbiome through probiotic administration. we hypothesized that lactobacilli displaying strong adhesion forces with path ... | 2012 | 22629342 |
| dfast and daga: web-based integrated genome annotation tools and resources. | quality assurance and correct taxonomic affiliation of data submitted to public sequence databases have been an everlasting problem. the ddbj fast annotation and submission tool (dfast) is a newly developed genome annotation pipeline with quality and taxonomy assessment tools. to enable annotation of ready-to-submit quality, we also constructed curated reference protein databases tailored for lactic acid bacteria. dfast was developed so that all the procedures required for ddbj submission could ... | 2016 | 27867804 |
| comparison of culture-dependent and culture-independent molecular methods for characterization of vaginal microflora. | to date, molecular methods that circumvent the limitations of traditional culture methods have not been used to describe the vaginal microflora in india. here, we compared culture and culture-independent molecular methods in characterizing the vaginal microbiota in indian women. | 2017 | 28260585 |
| comparison of culture-dependent and culture-independent molecular methods for characterisation of vaginal microflora. | to date, molecular methods that circumvent the limitations of traditional culture methods have not been used to describe the vaginal microflora in india. here, we compared culture and culture-independent molecular methods in characterising the vaginal microbiota in indian women. culture methods involved traditional cultivation on rogosa and sheep-blood agar, whereas culture-independent methods bypassed a culturing step by performing broad-range 16s rdna pcr on dna isolated directly from vaginal ... | 2016 | 27983477 |
| complete genome sequence of lactobacillus jensenii strain snuv360, a probiotic for treatment of bacterial vaginosis isolated from the vagina of a healthy korean woman. | lactobacillus jensenii snuv360 is a potential probiotic strain that shows antimicrobial activity for the treatment of bacterial vaginosis. here, we present the complete genomic sequence of l. jensenii snuv360, isolated from a vaginal sample from a healthy korean woman. analysis of the sequence may provide insight into its functional activity. | 2017 | 28280032 |
| draft genome sequence of the immunobiotic strain lactobacillus jensenii tl2937. | the genome of the immunomodulatory strain lactobacillus jensenii tl2937 is described here. the draft genome has a total length of 1,678,416 bp, a g+c content of 34.3%, and 1,470 predicted protein-coding sequences. the genome information will be useful for gaining insight into the immunomodulatory properties of the tl2937 strain in the porcine host. | 2017 | 28254966 |
| immunoregulatory effects triggered by immunobiotic lactobacillus jensenii tl2937 strain involve efficient phagocytosis in porcine antigen presenting cells. | immunobiotic lactobacillus jensenii tl2937 modulates porcine mononuclear phagocytes from peyer's patches (ppmps) and induces a differential production of pro- and anti-inflammatory cytokines in response to toll-like receptor (tlr)-4 activation. in view of the important role played by phagocytosis in the activation of antigen presenting cells (apcs), the aim of the present work was to examine the interaction of tl2937 with porcine ppmps focusing on phagocytosis. in addition, this study aimed to i ... | 2016 | 27342653 |
| robust vaginal colonization of macaques with a novel vaginally disintegrating tablet containing a live biotherapeutic product to prevent hiv infection in women. | mucocept is a biotherapeutic for prevention of hiv-1 infection in women and contains a human, vaginal lactobacillus jensenii that has been genetically enhanced to express the hiv-1 entry inhibitor, modified cyanovirin-n (mcv-n). the objective of this study was to develop a solid vaginal dosage form that supports sustained vaginal colonization of the mucocept lactobacillus at levels previously shown, with freshly prepared cultures, to protect macaques from shiv infection and to test this formulat ... | 2015 | 25875100 |
| endocarditis of the native aortic valve caused by lactobacillus jensenii. | lactobacilli are gram-positive anaerobic rods or coccobacilli, commonly found as commensals in human mucosa. rarely, they can cause serious infections such as infective endocarditis (ie), and the most frequently implicated species causing serious infections are l. casei and l. rhamnosus. ie caused by lactobacillus jensenii is very rare, with only six reported cases so far, to the best of our knowledge. we present a case of native aortic valve endocarditis caused by l. jensenii, complicated by ro ... | 2015 | 25750218 |