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filariasis and its control in fujian, china.epidemiological survey of filariasis in fujian province, china showed that malayan filariasis, transmitted by anopheles lesteri anthropophagus was mainly distributed in the northwest part and bancroftian filariasis with culex quinquefasciatus as vector, in middle and south coastal regions. both species of filariae showed typical nocturnal periodicity. involvement of the extremities was not uncommon in malayan filariasis. in contrast, hydrocele was often present in bancroftian filariasis, in whic ...19911948271
effectiveness of soap formulations containing deet and permethrin as personal protection against outdoor mosquitoes in malaysia.two soap formulations, both containing 20% deet and one each containing permethrin at 0.5 and 1.0%, respectively, were applied to exposed arms and legs of volunteers as personal protection against outdoor human biting mosquitoes in six locations on penang island, malaysia. the predominant mosquito species collected from these locations were aedes albopictus, mansonia uniformis, culex gelidus, anopheles lesteri and armigeres subalbatus. efficacy and residual effects up to 4 hours indicated good p ...19862906963
evaluation of permethrin-impregnated mosquito-nets against mosquitoes in china.in yishan county, guangxi province, china, where malaria and brugian filariasis are transmitted by vectors belonging to the anopheles hyrcanus pallas group, a study was conducted from june to november 1986 to evaluate the effectiveness of permethrin-impregnated mosquito bednets to reduce man/vector contact. in three experimental villages a total of 246 mosquito nets were treated with permethrin ec at a dosage of 0.5 g/m2. control bioassays (1 h exposure) of treated nets gave up to 100% mortality ...19882908784
[quantitative study on the role of anopheles lesteri anthropophagus in malaria transmission]. 19863539390
[distribution and ecology of anopheles lesteri, a human blood-sucking subspecies and its role in malaria transmission]. 19853912062
[studies on ddt residual spraying for controlling anopheles lesteri anthropophagus and plasmodium falciparum malaria]. 19846394169
[the role of anopheles lesteri anthropophagus in malaria transmission in jianghuai region, anhui]. 19846518629
[on the taxonomic status of anopheles anthropophagus and anopheles lesteri from china (diptera: culicidae)]. 200516296620
molecular evidence supports that anopheles anthropophagus from china and anopheles lesteri from japan are the same species.the hyrcanus group of anopheles consists of many related species, of which an. sinensis, an. lesteri, and an. anthropophagus are known as malaria vector species. it is not possible to identify these species morphologically in the adult and larval stages. nucleotide sequence alignment of 2nd internal transcribed spacer (its2) regions from 4 specimens of an. lesteri collected in japan, 2 specimens of an. anthropophagus collected in china, and 1 specimen of an. sinensis collected in korea were sequ ...200616878417
a locally acquired falciparum malaria via nosocomial transmission in korea.a 57-year old man who was admitted to an emergency room of a tertiary hospital with hemoptysis developed malarial fever 19 days later and then died from severe falciparum malaria 2 days later. he had not traveled outside of korea for over 30 years. through intensive interviews and epidemiological surveys, we found that a foreign patient with a recent history of travel to africa was transferred to the same hospital with severe falciparum malaria. we confirmed through molecular genotyping of the m ...200919724701
molecular epidemiological investigation of plasmodium knowlesi in humans and macaques in singapore.singapore reported its first locally acquired human plasmodium knowlesi infection in 2007, involving a soldier who had undergone training in a forested area where long-tailed macaques are frequently seen. comprehensive disease surveillance and monitoring system that was set up after the initial case detected four additional human p. knowlesi cases in 2007 and one in 2008. all involved military personnel who had undergone training in the forested area, and none had traveled out of singapore 1 mon ...201120586605
vectors and malaria transmission in deforested, rural communities in north-central vietnam.malaria is still prevalent in rural communities of central vietnam even though, due to deforestation, the primary vector anopheles dirus is uncommon. in these situations little is known about the secondary vectors which are responsible for maintaining transmission. basic information on the identification of the species in these rural communities is required so that transmission parameters, such as ecology, behaviour and vectorial status can be assigned to the appropriate species.201020846447
permanent genetic resources added to molecular ecology resources database 1 october 2009-30 november 2009.this article documents the addition of 411 microsatellite marker loci and 15 pairs of single nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: acanthopagrus schlegeli, anopheles lesteri, aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus oryzae, aspergillus terreus, branchiostoma japonicum, branchiostoma belcheri, colias behrii, coryphopterus personatus, cynogolssus semilaevis, cy ...201021565039
the dominant anopheles vectors of human malaria in the asia-pacific region: occurrence data, distribution maps and bionomic precis.abstract: background: the final article in a series of three publications examining the global distribution of 41 dominant vector species (dvs) of malaria is presented here. the first publication examined the dvs from the americas, with the second covering those species present in africa, europe and the middle east. here we discuss the 19 dvs of the asian-pacific region. this region experiences a high diversity of vector species, many occurring sympatrically, which, combined with the occurrence ...201121612587
high morphological and genetic variabilities of ochlerotatus scapularis, a potential vector of filarias and arboviruses.ochlerotatus scapularis is a potential vector of filarias and arboviruses in the neotropics. this species was once typically associated with sylvatic environments; however, cases of synanthropy and urbanization of this species have been increasingly reported in southeast brazil. despite the medical relevance of oc. scapularis, its populational variability is not yet known. to our knowledge, this is the first report describing the morphological and genetic variabilities of this species.201525885902
effects of microclimate condition changes due to land use and land cover changes on the survivorship of malaria vectors in china-myanmar border region.in the past decade, developing countries have been experiencing rapid land use and land cover changes, including deforestation and cultivation of previously forested land. however, little is known about the impact of deforestation and land-use changes on the life history of malaria vectors and their effects on malaria transmission. this study examined the effects of deforestation and crop cultivation on the adult survivorship of major malaria mosquitoes, anopheles sinensis and an. minimus in the ...201627171475
distribution of malaria vectors and incidence of vivax malaria at korean army installations near the demilitarized zone, republic of korea.as a result of the reintroduction of malaria in the republic of korea (rok) in 1993 and the threat to military and civilian populations, the korea military national defense (mnd) increased emphasis on vector control in 2012 at rok army (roka) installations located near the dmz, while decreasing chemoprophylaxis, fearing potential drug resistance. mosquito surveillance demonstrated a need for continuous monitoring of disease patterns among roka soldiers and vector malaria infection rates to ensur ...201627150110
diversification of the genus anopheles and a neotropical clade from the late cretaceous.the anopheles genus is a member of the culicidae family and consists of approximately 460 recognized species. the genus is composed of 7 subgenera with diverse geographical distributions. despite its huge medical importance, a consensus has not been reached on the phylogenetic relationships among anopheles subgenera. we assembled a comprehensive dataset comprising the coi, coii and 5.8s rrna genes and used maximum likelihood and bayesian inference to estimate the phylogeny and divergence times o ...201526244561
knockdown resistance of anopheles sinensis in henan province, china.vivax malaria was historically epidemic in henan province of china and anopheles sinensis was the main vectors and poor farming communities bare the greatest burden of disease. knockdown resistance in an. sinensis is one of the mechanisms of resistance against pyrethroids. in the present study, the frequency of mutations from an. sinensis was examined in henan province, china.201525890038
historical survey of the kdr mutations in the populations of anopheles sinensis in china in 1996-2014.anopheles sinensis has become an important malaria vector in china. the long-term extensive utilization of pyrethroids for itns and irs for mosquito control in the last three decades has resulted in the occurrence of resistant an. sinensis populations in many regions. knockdown resistance (kdr), caused by point mutations in the vgsc gene, is one of the mechanisms that confer resistance to ddt and pyrethroids. recently, several investigations revealed the kdr occurrence in some an. sinensis popul ...201525888824
malaria incidence from 2005-2013 and its associations with meteorological factors in guangdong, china.the temporal variation of malaria incidence has been linked to meteorological factors in many studies, but key factors observed and corresponding effect estimates were not consistent. furthermore, the potential effect modification by individual characteristics is not well documented. this study intends to examine the delayed effects of meteorological factors and the sub-population's susceptibility in guangdong, china.201525881185
mutations in the voltage-gated sodium channel gene of anophelines and their association with resistance to pyrethroids - a review.constant and extensive use of chemical insecticides has created a selection pressure and favored resistance development in many insect species worldwide. one of the most important pyrethroid resistance mechanisms is classified as target site insensitivity, due to conformational changes in the target site that impair a proper binding of the insecticide molecule. the voltage-gated sodium channel (nav) is the target of pyrethroids and ddt insecticides, used to control insects of medical, agricultur ...201425292318
decreasing incidence of plasmodium vivax in the republic of korea during 2010-2012.after the re-emergence of plasmodium vivax in 1993, a total of 31,254 cases of vivax malaria were reported between 1993-2012 in the republic of korea (rok). the purpose of this study was to review korea centers for disease control and prevention records to investigate the transmission of malaria from 2010-2012.201324010412
a potential threat to malaria elimination: extensive deltamethrin and ddt resistance to anopheles sinensis from the malaria-endemic areas in china.insecticide resistance in malaria vectors is a growing concern in many countries and requires immediate attention because of the limited chemical arsenal available for vector control. there is lack of systematic and standard monitoring data of malaria vector resistance in the endemic areas, which is essential for the ambitious goal of malaria elimination programme of china.201323683359
structural divergence of chromosomes between malaria vectors anopheles lesteri and anopheles sinensis.anopheles lesteri and anopheles sinensis are two major malaria vectors in china and southeast asia. they are dramatically different in terms of geographical distribution, host preference, resting habitats, and other traits associated with ecological adaptation and malaria transmission. both species belong to the anopheles hyrcanus group, but the extent of genetic differences between them is not well understood. to provide an effective way to differentiate between species and to find useful marke ...201627887641
spatial and space-time distribution of plasmodium vivax and plasmodium falciparum malaria in china, 2005-2014.despite the declining burden of malaria in china, the disease remains a significant public health problem with periodic outbreaks and spatial variation across the country. a better understanding of the spatial and temporal characteristics of malaria is essential for consolidating the disease control and elimination programme. this study aims to understand the spatial and spatiotemporal distribution of plasmodium vivax and plasmodium falciparum malaria in china during 2005-2009.201627993171
scanning electron microscopy of antennal sensilla of the eight anopheles species of the hyrcanus group (diptera: culicidae) in thailand.antennal sensilla were first investigated in the eight medically and veterinary important anopheles mosquito species (anopheles argyropus, anopheles crawfordi, anopheles nigerrimus, anopheles nitidus, anopheles paraliae (= anopheles lesteri), anopheles peditaeniatus, anopheles pursati, and anopheles sinensis) of the hyrcanus group in thailand, using scanning electron microscopy (sem). four types of sensilla, including sensilla chaetica (large and small), sensilla trichodea (sharp- and blunt-tipp ...201727752768
key knowledge gaps for plasmodium vivax control and elimination.there is inadequate understanding of the biology, pathology, transmission, and control of plasmodium vivax, the geographically most widespread cause of human malaria. during the last decades, study of this species was neglected, in part due to the erroneous belief that it is intrinsically benign. in addition, many technical challenges in culturing the parasite also hampered understanding its fundamental biology and molecular and cellular responses to chemotherapeutics. research on vivax malaria ...201627430544
spatiotemporal epidemic characteristics and risk factor analysis of malaria in yunnan province, china.malaria remains an important public health concern in china and is particularly serious in yunnan, a china's provincial region of high malaria burden with an incidence of 1.79/10(5) in 2012. this study aims to examine the epidemiologic profile and spatiotemporal aspects of epidemics of malaria, and to examine risk factors which may influence malaria epidemics in yunnan province.201728077125
genetic compatibility between anopheles lesteri from korea and anopheles paraliae from thailand.to assess differentiation and relationships between anopheles lesteri and anopheles paraliae we established three and five iso-female lines of an. lesteri from korea and an. paraliae from thailand, respectively. these isolines were used to investigate the genetic relationships between the two taxa by crossing experiments and by comparing dna sequences of ribosomal dna second internal transcribed spacer (its2) and mitochondrial dna cytochrome c oxidase subunit i (coi) and subunit ii (coii). resul ...023778670
Mosquito species composition and Plasmodium vivax infection rates on Baengnyeong-do (island), Republic of Korea.Vivax malaria is a significant military and civilian health threat in the north of the Republic of Korea (ROK). The island of Baengnyeong-do is the westernmost point of the ROK and is located close to the southwestern coast of the Democratic People's Republic of Korea (DPRK). Mosquitoes were collected using a black light trap on Baengnyeong-do, and Anopheles spp. were assayed by PCR, to identify the species, and screened for sporozoites of Plasmodium vivax. Of a subsample of 257 mosquitoes, Anop ...201122072836
the abundance and host-seeking behavior of culicine species (diptera: culicidae) and anopheles sinensis in yongcheng city, people's republic of china.abstract: background: the knowledge of mosquito species diversity and the level of anthropophily exhibited by each species in a region are of great importance to the integrated vector control. culicine species are the primary vectors of japanese encephalitis (je) virus and filariasis in china. anopheles sinensis plays a major role in the maintenance of plasmodium vivax malaria transmission in china. the goal of this study was to compare the abundance and host-seeking behavior of culicine specie ...201122115320
mitochondrial genetic differentiation across populations of the malaria vector anopheles lesteri from china (diptera: culicidae).abstract: background: anopheles lesteri is a primary vector of plasmodium spp. in central china. a complete understanding of vector population structure and the processes responsible for the differentiation is important to the vector-based malaria control programmes and for identifying heterogeneity in disease transmission as a result of discrete vector populations. there is no adequate an. lesteri population genetic data available. methods: polymorphism of sequence variations in mitochondrial c ...201121810272
population dynamics of five anopheles species of the hyrcanus group in northern gyeonggi-do, korea.to investigate the population densities of potential malaria vectors, anopheles species were collected by light traps in malaria endemic areas, paju and gimpo, gyeonggi-do of korea. five anopheles hyrcanus sibling species (an. sinensis, an. pullus, an. lesteri, an. kleini, and an. belenrae) were identified by pcr. the predominant species, an. pullus was collected during the late spring and mid-summer, while higher population consists of an. sinensis were collected from late summer to early autum ...201021234242
malaria vector surveillance in ganghwa-do, a malaria-endemic area in the republic of korea.we investigated the seasonality of anopheles mosquitoes, including its species composition, density, parity, and population densities of mosquitoes infected with the parasite in ganghwa-do (island), a vivax malaria endemic area in the republic of korea. mosquitoes were collected periodically with a dry-ice-tent trap and a blacklight trap during the mosquito season (april-october) in 2008. anopheles sinensis (94.9%) was the most abundant species collected, followed by anopheles belenrae (3.8%), a ...201020333283
the susceptibility of anopheles lesteri to infection with korean strain of plasmodium vivax.following its recent re-emergence, malaria has gained renewed attention as a serious infectious disease in korea. three species of the hyrcanusgroup, anopheles lesteri, anopheles sinensis and anopheles pullus, have long been suspected malaria vectors. however, opinions about their vector ability are controversial. the present study was designed with the aim of determining the susceptibility of these mosquitoes to a korean isolate of plasmodium vivax. also, an. sinensis is primarily suspected to ...200919284634
revisited its2 phylogeny of anopheles (anopheles) hyrcanus group mosquitoes: reexamination of unidentified and misidentified its2 sequences.the anopheles (anopheles) hyrcanus group of mosquitoes is very important for human health because some are malarial vector mosquitoes. despite their pathological importance, some unidentified internal transcribed spacer 2 (its2) sequences have been reported from members of this group, and their phylogenetic relationships have been rarely understood. in the present study, 84 its2 sequences for the hyrcanus group members were retrieved from genbank. the detailed sequence comparison unambiguously r ...200717514381
identification and characterization of larval and adult anopheline mosquito habitats in the republic of korea: potential use of remotely sensed data to estimate mosquito distributions.plasmodium vivax malaria reemerged in the republic of korea in 1993, with more than 2,000 cases reported in the northwestern part of the country over the last 10 years. to better assess the risk of malaria transmission we conducted a surveillance study to identify and characterize the habitats that produce potential anopheles vector mosquitoes. immature and adult mosquito collection data were incorporated into a geographic information system (gis) along with remotely sensed satellite imagery, an ...200516011809
[on molecular identification and taxonomic status of anopheles lesteri and anopheles anthropophagus in china (diptera: culicidae)].to clarify the taxonomic status of anopheles lesteri and an. anthropophagus in china.200012567602
late season commercial mosquito trap and host seeking activity evaluation against mosquitoes in a malarious area of the republic of korea.field trials evaluating selected commercially available mosquito traps variously baited with light, carbon dioxide, and/or octenol were conducted from 18-27 september 2000 in a malarious area near paekyeon-ri (tongil-chon) and camp greaves in paju county, kyonggi province, republic of korea. the host-seeking activity for common mosquito species, including the primary vector of japanese encephalitis, culex tritaeniorhynchus giles, was determined using hourly aspirator collections from a human and ...200211949213
vector competence of anopheles lesteri baisas and hu (diptera: culicidae) to plasmodium vivax in korea.three anopheline mosquitoes in korea were studied for their abilities as vectors for plasmodium vivax. the female mosquitoes of anopheles lesteri, an. pullus and an. sinensis were allowed to suck malaria patient blood until fully fed, and they were then bred for 2 weeks to develop from malaria parasites to sporozoites. the result from the above confirmed the sporozoites in one an. lesteri of one individual and five an. sinensis of six individuals. we also confirmed that an. sinensis was the main ...200211949212
[geographical distribution of anopheles lesteri anthropophagus and its role in malaria transmission in guangxi]. 19873652409
[vectorial capacity of anopheles lesteri anthropophagus in transmitting filariasis malayi in leshan prefecture, sichuan province]. 19873447779
a mark-release-recapture experiment with anopheles lesteri paraliae in northwest peninsular malaysia.in a coastal village in northwest malaysia, 3231 fed anopheles females of eight to 10 species were collected, marked with fluorescent dust, and released on three consecutive nights. in collections made on the 10 nights after the first release, 58 mosquitoes of three species, an. lesteri paraliae, an. subpictus and an. vagus, were recaptured; the recapture rates were 3.42%, 1.19% and 0.97%, respectively. the data for an. subpictus and an. vagus were insufficient for further analysis. those for an ...19921463364
species composition, larval habitats, seasonal occurrence and distribution of potential malaria vectors and associated species of anopheles (diptera: culicidae) from the republic of korea.larval mosquito habitats of potential malaria vectors and related species of anopheles from three provinces (gyeonggi, gyeongsangbuk, chungcheongbuk provinces) of the republic of korea were surveyed in 2007. this study aimed to determine the species composition, seasonal occurrence and distributions of anopheles mosquitoes. satellite derived normalized difference vegetation index data (ndvi) was also used to study the seasonal abundance patterns of anopheles mosquitoes.201020163728
random repeated cross sectional study on breeding site characterization of anopheles sinensis larvae in distinct villages of yongcheng city, people's republic of china.characterizing the breeding site of anopheles sinensis is of major importance for the transition from malaria control to elimination in china. however, little information is available especially regarding the characteristics and influencing factors of breeding sites of an. sinensis in yongcheng city, a representative region of unstable malaria transmission in the huang-huai river region of central china. the aims of this study were to determine the breeding site characteristics of an. sinensis a ...201222444032
the polymorphism and the geographical distribution of the knockdown resistance (kdr) of anopheles sinensis in the republic of korea.in the republic of korea (rok), six sibling species of the anopheles sinensis complex are considered the vector species of malaria, but data on their susceptibilities to malaria and vector capacities have been controversial. the intensive use of insecticides has contributed to the rapid development and spread of insecticide resistance in the an. sinensis complex. knockdown resistance (kdr) to pyrethroids and ddt in the an. sinensis complex is associated with a mutation in codon 1014 of the volta ...201222554130
the effects of urbanization on global plasmodium vivax malaria transmission.many recent studies have examined the impact of urbanization on plasmodium falciparum malaria endemicity and found a general trend of reduced transmission in urban areas. however, none has examined the effect of urbanization on plasmodium vivax malaria, which is the most widely distributed malaria species and can also cause severe clinical syndromes in humans. in this study, a set of 10,003 community-based p. vivax parasite rate (pvpr) surveys are used to explore the relationships between pvpr i ...201223217010
mosquito species composition and plasmodium vivax infection rates for korean army bases near the demilitarized zone in the republic of korea, 2011.vivax malaria is a significant military and civilian health threat in northern republic of korea (rok). mosquito collections were performed at two rok army installations, paju near the demilitarized zone (dmz) using black light traps in 2011. the dmz, a 4 km wide border, is the northernmost point of the rok and separates the rok from the democratic people's republic of korea (dprk). anopheles spp. were identified by polymerase chain reaction and screened for plasmodium vivax sporozoites. of 4,35 ...201223243112
susceptibility and resistance of field populations of anopheles sinensis (diptera: culicidae) collected from paju to 13 insecticides.over 20% of all malaria cases reported annually in the republic of korea (rok) occur in paju, gyeonggi province. vector control for malaria management is essential, but the insecticide resistance of the vector, anopheles mosquitoes, has been a major obstacle in implementing effective control. in this study, the insecticide resistance of the vector mosquitoes was evaluated and compared with that of vector mosquitoes collected from the same locality in 2001 and 2009.024159535
susceptibility of five species members of the korean hyrcanus group to brugia malayi, and hybridization between b. malayi-susceptible and -refractory anopheles sinensis strains.five species members of the korean hyrcanus group: anopheles pullus, anopheles sinensis, anopheles kleini, anopheles belenrae, and anopheles lesteri were tested for susceptibility to brugia malayi. they were allowed to feed artificially on blood containing b. malayi microfilariae and dissected 14 days after feeding. the susceptibility rates were 60%, 65%, 90%, 100% and 100% in an. pullus, an. sinensis, an. kleini, an. belenrae, and an. lesteri, respectively. as determined by levels of susceptibi ...201424974643
mosquitoes and other aquatic insects in fallow field biotopes and rice paddy fields.fallow field biotopes that develop from abandoned rice fields are man-made wetlands that provide new habitats for various aquatic animals. although consideration of such biotopes generally focuses on their positive aspects, this study evaluated the negative aspects of establishing fallow field biotopes with regard to mosquito breeding sites. to determine whether fallow field biotopes become breeding habitats for vector mosquitoes, we evaluated mosquito fauna in fallow field biotopes and adjacent ...201323167444
anopheles vectors in mainland china while approaching malaria elimination.china is approaching malaria elimination; however, well-documented information on malaria vectors is still missing, which could hinder the development of appropriate surveillance strategies and who certification. this review summarizes the nationwide distribution of malaria vectors, their bionomic characteristics, control measures, and related studies. after several years of effort, the area of distribution of the principal malaria vectors was reduced, in particular for anopheles lesteri (synony ...201728734898
immune responses of aedes togoi, anopheles paraliae and anopheles lesteri against nocturnally subperiodic brugia malayi microfilariae during migration from the midgut to the site of development.lymphatic filariasis is a mosquito-borne disease caused by filarioid nematodes. a comparative understanding of parasite biology and host-parasite interactions can provide information necessary for developing intervention programmes for vector control. here, to understand such interactions, we choose highly susceptible filariasis vectors (aedes togoi and anopheles lesteri) as well as anopheles paraliae, which has lower susceptibility, infected them with nocturnally subperiodic (nsp) brugia malayi ...201830261926
effects of cross-mating on susceptibility of synonymous mosquitoes, anopheles paraliae and anopheles lesteri to infection with nocturnally subperiodic brugia malayi.in southeast asia, anopheles lesteri (recently synonymized with an. paraliae) is a competent vector for plasmodium parasites, but its ability to transmit parasites that cause lymphatic filariasis has yet to be determined. in this study, the susceptibility of an. lesteri and an. paraliae to brugia malayi parasites was determined by comparing with the control mosquito, aedes togoi. we found that the infection prevalence per infected mosquito in an. paraliae was significantly lower than that in ae. ...201830055175
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