| [cloning of zfy /zfx gene of the tufted deer and identification of its sex]. | according to the human sex differentiation related zfy and zfx genes, a pair of primers were designed , and fragments were amplified from the genomic dna of male or female tufted deer. subsequently the amplified fragments were cloned into the vector pmd18t and were sequenced. it is found that the sequences of zfy gene and zfx gene have 91% homology. based on the different nucleotides, restriction site of ava ii was found to be specific to zfx gene. the results show that the combination of pcr wi ... | 2004 | 15640041 |
| phylogenomics of several deer species revealed by comparative chromosome painting with chinese muntjac paints. | a set of chinese muntjac (muntiacus reevesi) chromosome-specific paints has been hybridized onto the metaphases of sika deer (cervus nippon, cni, 2n = 66), red deer (cervus elaphus, cel, 2n = 62) and tufted deer (elaphodus cephalophus, ece, 2n = 47). thirty-three homologous autosomal segments were detected in genomes of sika deer and red deer, while 31 autosomal homologous segments were delineated in genome of tufted deer. the chinese muntjac chromosome x probe painted to the whole x chromosome, ... | 2006 | 16850210 |
| polymorphic karyotypes and sex chromosomes in the tufted deer (elaphodus cephalophus): cytogenetic studies and analyses of sex chromosome-linked genes. | different diploid chromosome numbers have been reported for the tufted deer elaphodus cephalophus (female, 2n = 46/47; male, 2n = 47/48) in earlier reports. in the present study, chromosomal analysis of seven tufted deer (5 male symbol, 2 female symbol) revealed that the karyotype of these animals contains 48 chromosomes, including a pair of large heteromorphic chromosomes in the male. c-banding revealed these chromosomes to be very rich in constitutive heterochromatin. chromosome banding and pc ... | 2005 | 15905647 |
| complete mitochondrial genome of northern indian red muntjac (muntiacus vaginalis) and its phylogenetic analysis. | we report complete mitochondrial genome of northern indian red muntjac, muntiacus vaginalis, and its phylogenetic inferences. mitogenome composition was 16,352 bp in length and its overall base composition in the circular genome was a = 33.2%, t = 29.0%, c = 24.50% and g = 13.30%. it exhibited a typical mitogenome structure, including 22 transfer rna genes, 13 protein-coding genes, two ribosomal rna genes and a major non-coding control region (d-loop region). all the genes except nd6 and eight t ... | 2019 | 30456740 |
| diversity and activity patterns of sympatric animals among four types of forest habitat in guanyinshan nature reserve in the qinling mountains, china. | environmental heterogeneity contributes to various habitats and may influence the diversity and activity patterns of wildlife among habitats. we used camera traps to assess wildlife habitat use in guanyinshan nature reserve from 2009 to 2012. we focused on four types of habitat including open areas with gentle slope (<15°) (type1), low elevation areas (about 1500-1700 m) with high bamboo coverage (type2), high elevation areas (about 2100-2300 m) with high canopy coverage (type3), and wildlife mi ... | 2017 | 28551745 |
| the chromosomes of tufted deer (elaphodus cephalophus). | mitotic and meiotic chromosome preparations of the tufted deer (elaphodus cephalophus) were studied to elucidate the sex-chromosomal polymorphism evidenced by this species. females had 2n = 46 or 47 chromosomes, whereas males had 2n = 47 or 48 chromosomes. an x;autosome translocation was identified by synaptonemal complex analysis of spermatocytes at pachytene and confirmed by the presence of a trivalent at diakinesis/metaphase i. the present work, in combination with earlier observations by oth ... | 1991 | 2055116 |
| [phylogenetic relationship between tufted deer(elaphodus cephalophus) and muntiacus deer is revealed by the exon and intron of k+ channel gene]. | in this study, partial fragments of potassium ion channel gene were amplified using the genomic dna of muntjak, reevesi, crinifrons, and elaphodus cephalophus. the pcr products were ligated to the plasmid of pmd18-t vector by the method of direct t-a cloning. the positive clones were identified by colony pcr. the sequences of the recombinant clones were determined using m13-47/rv-m universal primers and aligned by the software clustalw. the nucleotide divergences of exon were 0.90%-1.44% among t ... | 2005 | 15730968 |
| identification of complete mitochondrial genome of the tufted deer. | the tufted deer elaphodus cephalophus are endangered animals in the world and little is understood about their mitochondrial (mt) genome. in our study, the mt genome of the tufted deer is identified--which is about 16 kb in length and contains 13 protein-coding genes, two ribosomal rna genes, 22 transfer rna genes and a non-coding sequence (control region). the distinguishing feature is that gtg is the start codon of the nadh4l gene and the cyt b gene has a subterminal aaa followed by the stop c ... | 2008 | 19462515 |
| reproductive seasonality and sperm cryopreservation in the male tufted deer (elaphodus cephalophus). | the tufted deer is a small deer, listed as near threatened on the international union for conservation of nature red list, and there is no information on the fundamental reproductive biology of this species. in this study, we report for the first time, characterization of male reproductive traits and cryopreservation of semen in this species. males were subjected to electroejaculation during each season (autumn, winter, spring, and summer), and ejaculates were assessed for motility and quality t ... | 2016 | 27125695 |
| molecular detection of anaplasma phagocytophilum in wild cervids and hares in china. | anaplasma phagocytophilum is an emerging tick-borne pathogen of global veterinary and public health significance. wild ungulates are suggested to serve as reservoirs for this agent in both the us and europe. we investigated the occurrence of a. phagocytophilum in sika deer ( cervus nippon ), reeves' muntjac ( muntiacus reevesi ), tufted deer ( elaphodus cephalophus ), and chinese hare ( lepus sinensis ) in china. we detected a. phagocytophilum in nine (53%) free-ranging wild reeves' muntjac. no ... | 2017 | 28151084 |
| yangtze river, an insignificant genetic boundary in tufted deer (elaphodus cephalophus): the evidence from a first population genetics study. | great rivers were generally looked at as the geographical barrier to gene flow for many taxonomic groups. the yangtze river is the third largest river in the world, and flows across south china and into the east china sea. up until now, few studies have been carried out to evaluate its effect as a geographical barrier. in this study, we attempted to determine the barrier effect of the yangtze river on the tufted deer (elaphodus cephalophus) using the molecular ecology approach. using mitochondri ... | 2016 | 27843712 |
| isolation and characterization of the cetn1 gene from tufted deer (elaphodus cephalophus). | the tufted deer, a species found only in china, has polymorphic sex chromosomes (2n = 46, 47, 48). centrins are centrosome components in species from yeast to humans. here, the cetn1 gene was isolated from the testis cdna library of tufted deer, and its expression pattern, number of gene copies, and gene structure were studied. to date, for unknown reasons, satisfactory genomic dna of the tufted deer has not been obtained. in the present study, we extracted high molecular weight genomic dna from ... | 2008 | 18751886 |