| susceptibility of rickettsia monacensis and rickettsia peacockii to cecropin a, ceratotoxin a, and lysozyme. | ticks host obligate intracellular bacteria that range from benign symbiotes to virulent human pathogens. the effects on those bacteria of antimicrobial peptides (amps) involved in arthropod innate immunity to microbial infections are largely unknown. we evaluated effects of amps and a c-type lysozyme on host cell-free suspensions of the tick symbiotes rickettsia monacensis and rickettsia peacockii with stain-based infectivity and viability assays. cecropin a at a concentration of 8 mum: had a le ... | 2005 | 16132458 |
| detection of borrelia lusitaniae, rickettsia sp. irs3, rickettsia monacensis, and anaplasma phagocytophilum in ixodes ricinus collected in madeira island, portugal. | a total of 300 ixodes ricinus ticks were tested by polymerase chain reaction (pcr) for the presence of borrelia spp., rickettsia spp., and anaplasma phagocytophilum. sequence analysis demonstrated 8 (2.7%) ticks infected with b. lusitaniae, 60 (20%) with rickettsia spp., and 1 (0.3%) with a. phagocytophilum. seven (2.3%) ticks were coinfected with b. lusitaniae and rickettsia spp., 2 (0.6%) with r. monacensis, and 5 (1.7%) with rickettsia sp. irs3. the results of this study suggest simultaneous ... | 2008 | 18454598 |
| role of birds in dispersal of etiologic agents of tick-borne zoonoses, spain, 2009. | we amplified gene sequences from anaplasma phagocytophilum, borrelia garinii, b. valaisiana, b. turdi, rickettsia monacensis, r. helvetica, r. sibirica sibirica, and rickettsia spp. (including candidatus rickettsia vini) in ticks removed from birds in spain. the findings support the role of passerine birds as possible dispersers of these tick-borne pathogens. | 2012 | 22709801 |
| role of the lizard teira dugesii as a potential host for ixodes ricinus tick-borne pathogens. | pcr screening of ticks and tissue samples collected from 151 teira dugesii lizards seems to indicate a potential role of this lizard species in the maintenance and transmission cycle of some ixodes ricinus tick-borne agents, such as rickettsia monacensis, rickettsia helvetica, and borrelia lusitaniae, that are circulating on madeira island. | 2012 | 22407681 |
| vector competence of the tick ixodes sinensis (acari: ixodidae) for rickettsia monacensis. | cases of mediterranean spotted fever like rickettsioses, caused by rickettsia monacensis, have become more common in the last 10 years. in china, natural infection of r. monacensis in various tick species has been confirmed but the vector(s) of r. monacensis have not been recorded. | 2014 | 25406413 |
| infections and coinfections of questing ixodes ricinus ticks by emerging zoonotic pathogens in western switzerland. | in europe, ixodes ricinus is the vector of many pathogens of medical and veterinary relevance, among them borrelia burgdorferi sensu lato and tick-borne encephalitis virus, which have been the subject of numerous investigations. less is known about the occurrence of emerging tick-borne pathogens like rickettsia spp., babesia spp., "candidatus neoehrlichia mikurensis," and anaplasma phagocytophilum in questing ticks. in this study, questing nymph and adult i. ricinus ticks were collected at 11 si ... | 2012 | 22522688 |
| development of shuttle vectors for transformation of diverse rickettsia species. | plasmids have been identified in most species of rickettsia examined, with some species maintaining multiple different plasmids. three distinct plasmids were demonstrated in rickettsia amblyommii aar/sc by southern analysis using plasmid specific probes. copy numbers of pram18, pram23 and pram32 per chromosome in aar/sc were estimated by real-time pcr to be 2.0, 1.9 and 1.3 respectively. cloning and sequencing of r. amblyommii aar/sc plasmids provided an opportunity to develop shuttle vectors fo ... | 2011 | 22216299 |
| first detection of spotted fever group rickettsiae in ticks in serbia. | ticks can transmit multiple pathogenic bacteria responsible for diseases in animals and humans such as borrelia burgdorferi sensu lato, anaplasma phagocytophilum, and spotted fever group rickettsia sp. the current study aimed to investigate the presence of rickettsiae in ticks collected from seven localities in serbia. one hundred thirty-one (131) questing ticks belonging to 5 tick species (dermacentor marginatus, dermacentor reticulatus, haemaphysalis punctata, haemaphysalis concinna, and ixode ... | 2010 | 21142961 |
| established and emerging pathogens in ixodes ricinus ticks collected from birds on a conservation island in the baltic sea. | tick-borne pathogens such as lyme borreliosis spirochaetes, anaplasma phagocytophilum, rickettsia spp. and babesia spp. cause a great variety of diseases in animals and humans. although their importance with respect to emerging human diseases is increasing, many issues about their ecology are still unclear. in spring 2007, 191 ixodes ricinus (acari: ixodidae) ticks were collected from 99 birds of 11 species on a bird conservation island in the baltic sea in order to test them for borrelia spp., ... | 2010 | 20868431 |
| co-circulation of emerging tick-borne pathogens in middle germany. | abstract from may until october 2007, a total of 658 ixodes ricinus ticks were collected off birds (189), rodents (273), and vegetation (196) in a certain area of middle germany and investigated for infection with babesia spp., anaplasma phagocytophilum, and rickettsia spp. overall, 13.1% (86/658) of the ticks were infected with at least one pathogen; co-infections occurred in 0.6% (4/658). babesia spp. specific dna was detected in 9.7% (64/658) of the ticks, 1.4% (9/658) were infected with a. p ... | 2011 | 20846013 |
| detection of babesia sp. eu1 and members of spotted fever group rickettsiae in ticks collected from migratory birds at curonian spit, north-western russia. | to reveal the prevalence of spotted fever group (sfg) rickettsiae and babesia sp. in ixodes ricinus (l.) ticks from migratory birds, 236 specimens represented 8 species of passeriformes and were collected at curonian spit in kaliningrad enclave of north-western russia. the ticks (total 126) being detached from four bird species, turdus philomelos, fringilla coelebs, parus major, and sturnus vulgaris, were investigated by pcr using the primers rp cs.877p/rp cs.1258n for the detection of rickettsi ... | 2011 | 20553110 |
| detection of rickettsia and anaplasma from hard ticks in thailand. | we collected a total of 169 adult hard ticks and 120 nymphs from under the leaves of plants located along tourist nature trails in ten localities. the results present data examining the vector competence of ticks of different genera and the presence of rickettsia and anaplasma species. the ticks belonged to three genera, amblyomma, dermacentor, and haemaphysalis, comprising 11 species. rickettsia bacteria were detected at three collection sites, while anaplasma bacteria were detected at only one ... | 2015 | 26611960 |
| molecular evidence of spotted fever group rickettsiae and anaplasmataceae from ticks and stray dogs in bangladesh. | emerging tick-borne diseases (tbds) are important foci for human and animal health worldwide. however, these diseases are sometimes over looked, especially in countries with limited resources to perform molecular-based surveys. the aim of this study was to detect and characterize spotted fever group (sfg) rickettsiae and anaplasmataceae in bangladesh, which are important tick-borne pathogens for humans and animals worldwide. a total of 50 canine blood samples, 15 ticks collected from dogs, and 1 ... | 2016 | 26573516 |
| dissemination of spotted fever rickettsia agents in europe by migrating birds. | migratory birds are known to play a role as long-distance vectors for many microorganisms. to investigate whether this is true of rickettsial agents as well, we characterized tick infestation and gathered ticks from 13,260 migratory passerine birds in sweden. a total of 1127 ixodes spp. ticks were removed from these birds and the extracted dna from 957 of them was available for analyses. the dna was assayed for detection of rickettsia spp. using real-time pcr, followed by dna sequencing for spec ... | 2010 | 20052286 |
| typhus and other rickettsioses: emerging infections in germany. | rickettsioses are diseases caused by rickettsiae, obligate intracellular bacteria that are transmitted by arthropods to humans. they cause various types of spotted fever and typhus. | 2009 | 19547738 |
| first description of rickettsia monacensis in ixodes ricinus in algeria. | | 2009 | 19486071 |
| molecular investigations of rickettsia helvetica infection in dogs, foxes, humans, and ixodes ticks. | rickettsia helvetica, a tick-borne member of the spotted-fever-group rickettsiae, is a suspected pathogen in humans; however, its role in animals is unknown. the aims of this study were to establish a r. helvetica-specific real-time taqman pcr assay and apply it to the analysis of tick vectors (to determine potential exposure risk) and blood samples from canidae and humans (to determine prevalence of infection). the newly designed 23s rrna gene assay for r. helvetica was more sensitive than a pu ... | 2009 | 19329665 |
| first report of rickettsia monacensis and rickettsia helvetica from tunisia. | | 2008 | 18782496 |
| rickettsia monacensis and human disease, spain. | we identified rickettsia monacensis as a cause of acute tickborne rickettsiosis in 2 humans. its pathogenic role was assessed by culture and detection of the organism in patients' blood samples. this finding increases the number of recognized human rickettsial pathogens and expands the known geographic distribution of mediterranean spotted fever-like cases. | 2007 | 18252123 |
| transposon insertion reveals prm, a plasmid of rickettsia monacensis. | until the recent discovery of prf in rickettsia felis, the obligate intracellular bacteria of the genus rickettsia (rickettsiales: rickettsiaceae) were thought not to possess plasmids. we describe prm, a plasmid from rickettsia monacensis, which was detected by pulsed-field gel electrophoresis and southern blot analyses of dna from two independent r. monacensis populations transformed by transposon-mediated insertion of coupled green fluorescent protein and chloramphenicol acetyltransferase mark ... | 2007 | 17575002 |
| infection of ixodes scapularis ticks with rickettsia monacensis expressing green fluorescent protein: a model system. | ticks (acari: ixodidae) are ubiquitous hosts of rickettsiae (rickettsiaceae: rickettsia), obligate intracellular bacteria that occur as a continuum from nonpathogenic arthropod endosymbionts to virulent pathogens of both arthropod vectors and vertebrates. visualization of rickettsiae in hosts has traditionally been limited to techniques utilizing fixed tissues. we report epifluorescence microscopy observations of unfixed tick tissues infected with a spotted fever group endosymbiont, rickettsia m ... | 2007 | 17125789 |
| are apodemus spp. mice and myodes glareolus reservoirs for borrelia miyamotoi, candidatus neoehrlichia mikurensis, rickettsia helvetica, r. monacensis and anaplasma phagocytophilum? | in europe, in addition to borrelia burgdorferi sensu lato and tick-borne encephalitis (tbe) virus, other zoonotic pathogens, like b. miyamotoi, a species related to the relapsing fever spirochaetes, candidatus neoehrlichia mikurensis (n. mikurensis), rickettsia helvetica, rickettsia monacensis, and anaplasma phagocytophilum have been reported in the ixodid tick ixodes ricinus. no study was conducted to identify reservoir hosts for these pathogens. here, we investigated the role played by wild ro ... | 2014 | 24582511 |
| rickettsiae of the spotted-fever group in ixodid ticks from hungary: identification of a new genotype ('candidatus rickettsia kotlanii'). | three common european 'anthrophilic' ticks, ixodes ricinus, haemaphysalis concinna and dermacentor reticulatus, were collected in hungary and tested, in assays based on nested pcr, for rickettsiae of the spotted-fever group. low percentages of i. ricinus (2.7%) and h. concinna (1.0%) and a high percentage of d. reticulatus (26.8%) were found to be infected. the rickettsiae in the ticks were then identified, by sequencing of the genes coding for 16s ribosomal rna (16s rdna), citrate synthase (glt ... | 2006 | 16630380 |
| analysis of fluorescent protein expression in transformants of rickettsia monacensis, an obligate intracellular tick symbiont. | we developed and applied transposon-based transformation vectors for molecular manipulation and analysis of spotted fever group rickettsiae, which are obligate intracellular bacteria that infect ticks and, in some cases, mammals. using the epicentre ez::tn transposon system, we designed transposons for simultaneous expression of a reporter gene and a chloramphenicol acetyltransferase (cat) resistance marker. transposomes (transposon-transposase complexes) were electroporated into rickettsia mona ... | 2005 | 15812043 |
| study on ticks and tick-borne zoonoses in public parks in italy. | a survey on tick density and on tick-borne zoonoses was carried out in four public parks in the outskirts of imola (northern italy) from june to october 2006. all stages of ixodes ricinus and only larvae of riphicephalus sanguineus were recovered by dragging, performed on 100-m transects. almost all ticks (99%) were harvested in one park. i. ricinus density (nymphs/100 m(2) ) ranged from 0 in park l to 6.3 in park f. nymphs and adults of i. ricinus were subjected to pcr for anaplasma phagocytoph ... | 2012 | 22551055 |
| rickettsia monacensis sp. nov., a spotted fever group rickettsia, from ticks (ixodes ricinus) collected in a european city park. | we describe the isolation and characterization of rickettsia monacensis sp. nov. (type strain, irr/munich(t)) from an ixodes ricinus tick collected in a city park, the english garden in munich, germany. rickettsiae were propagated in vitro with ixodes scapularis cell line ise6. blast analysis of the 16s rrna, the citrate synthase, and the partial 190-kda rickettsial outer membrane protein a (rompa) gene sequences demonstrated that the isolate was a spotted fever group (sfg) rickettsia closely re ... | 2002 | 12200314 |
| rickettsia helvetica and r. monacensis infections in immature ixodes ricinus ticks derived from sylvatic passerine birds in west-central poland. | the aim of this study was to assess the importance of forest passerine birds in spreading ixodid ticks infected with rickettsiae of spotted fever group (sfg) in sylvatic habitats in western poland. in total, 834 immature ixodes ricinus ticks were found on 64 birds of 11 species which were captured during the tick-questing season between may and september of 2006. ground-foraging passerines hosted most of the ticks compared with arboreal species, and therefore, only the former group was included ... | 2016 | 27164834 |
| molecular detection of tick-borne pathogens in humans with tick bites and erythema migrans, in the netherlands. | tick-borne diseases are the most prevalent vector-borne diseases in europe. knowledge on the incidence and clinical presentation of other tick-borne diseases than lyme borreliosis and tick-borne encephalitis is minimal, despite the high human exposure to these pathogens through tick bites. using molecular detection techniques, the frequency of tick-borne infections after exposure through tick bites was estimated. | 2016 | 27706159 |
| investigation of tick-borne bacteria (rickettsia spp., anaplasma spp., ehrlichia spp. and borrelia spp.) in ticks collected from andean tapirs, cattle and vegetation from a protected area in ecuador. | ixodid ticks play an important role in the transmission and ecology of infectious diseases. information about the circulation of tick-borne bacteria in ticks is lacking in ecuador. our aims were to investigate the tick species that parasitize andean tapirs and cattle, and those present in the vegetation from the buffer zone of the antisana ecological reserve and cayambe-coca national park (ecuador), and to investigate the presence of tick-borne bacteria. | 2015 | 25616567 |
| avian migrants facilitate invasions of neotropical ticks and tick-borne pathogens into the united states. | migratory birds have the potential to transport exotic vectors and pathogens of human and animal health importance across vast distances. we systematically examined birds that recently migrated to the united states from the neotropics for ticks. we screened both ticks and birds for tick-borne pathogens, including rickettsia species and borrelia burgdorferi. over two spring seasons (2013 and 2014), 3.56% of birds (n = 3,844) representing 42.35% of the species examined (n = 85) were infested by ti ... | 2015 | 26431964 |
| broad-range survey of tick-borne pathogens in southern germany reveals a high prevalence of babesia microti and a diversity of other tick-borne pathogens. | abstract ticks harbor numerous pathogens of significance to human and animal health. a better understanding of the pathogens carried by ticks in a given geographic area can alert health care providers of specific health risks leading to better diagnosis and treatments. in this study, we tested 226 ixodes ricinis ticks from southern germany using a broad-range pcr and electrospray ionization mass spectrometry assay (pcr/esi-ms) designed to identify tick-borne bacterial and protozoan pathogens in ... | 2014 | 25072989 |
| borrelia garinii and rickettsia monacensis in ixodes ricinus ticks, algeria. | | 2014 | 25272139 |
| pathogens in ticks collected from dogs in berlin/brandenburg, germany. | tick-borne diseases are a major health risk for humans and dogs. in addition to collection and analysis of questing ticks, analysis of host-associated ticks for the presence of pathogens is a valuable method to gain insight into transmission patterns of tick-borne diseases. | 2014 | 25441762 |
| co-infection of borrelia burgdorferi sensu lato and rickettsia species in ticks and in an erythema migrans patient. | lyme borreliosis is the most prevalent tick-borne disease in europe. ixodes ricinus also carries other pathogenic bacteria, but corresponding human diseases are rarely reported. here, we compared the exposure to rickettsia helvetica and rickettsia monacensis with that to lyme borreliosis spirochetes. we assumed that their exposure corresponds to their infection rate in questing i. ricinus. | 2013 | 24326096 |
| detection of spotted fever group rickettsiae in ticks from zhejiang province, china. | tick species distribution and prevalence of spotted fever group rickettsiae (sfgr) in ticks were investigated in zhejiang province, china in 2010 and 2011. pcr was used to detect sfgr and positive amplicons were sequenced, compared to published sequences and phylogenic analysis was performed using mega 4.0. a total of 292 adult ticks of ten species were captured and 7.5 % (22/292) of the ticks were pcr-positive for sfg rickettsia. the pcr-positive rates were 5.5 % (6/110) for haemaphysalis longi ... | 2015 | 25633265 |
| arsenophonus nasoniae and rickettsiae infection of ixodes ricinus due to parasitic wasp ixodiphagus hookeri. | arsenophonus nasoniae, a male-killing endosymbiont of chalcid wasps, was recently detected in several hard tick species. following the hypothesis that its presence in ticks may not be linked to the direct occurrence of bacteria in tick's organs, we identified a. nasoniae in wasps emerging from parasitised nymphs. we confirmed that 28.1% of ixodiphagus hookeri wasps parasitizing ixodes ricinus ticks were infected by a. nasoniae. moreover, in examined i. ricinus nymphs, a. nasoniae was detected on ... | 2016 | 26901622 |
| rickettsia species in ticks removed from humans in istanbul, turkey. | a total of 167 ticks collected from humans in istanbul (turkey) in 2006 were screened for rickettsia species, and nested pcrs targeting glta and ompa rickettsial fragment genes were carried out. rickettsia monacensis (51), r. aeschlimannii (8), r. conorii subsp. conorii (3), r. helvetica (2), r. raoultii (1), r. africae (1), r. felis (1), and other rickettsia spp. (2), were detected. to our knowledge, these rickettsia species (except r. conorii) had never been reported in ticks removed from huma ... | 2012 | 22925016 |
| rickettsia monacensis as cause of mediterranean spotted fever-like illness, italy. | | 0 | 22469314 |
| motility characteristics are altered for rickettsia bellii transformed to overexpress a heterologous ricka gene. | the rickettsial protein ricka activates host cell factors associated with the eukaryotic actin cytoskeleton and is likely involved with rickettsial host cell binding and infection and the actin-based motility of spotted fever group rickettsiae. the ricka gene sequence and protein vary substantially between rickettsia species, as do observed motility-associated phenotypes. to help elucidate the function of ricka and determine the effects of species-specific ricka variations, we compared extracell ... | 2013 | 24296498 |
| urban breeding corvids as disseminators of ticks and emerging tick-borne pathogens. | crows (corvidae) are common city dwellers worldwide and are increasingly important subjects of epidemiology studies. although their importance as hosts and transmitters of a number of zoonotic parasites and pathogens is well known, there are no studies on their importance as tick hosts. after mosquitoes, ticks are the most important vectors of zoonotic pathogens, especially for those causing emerging zoonotic diseases. pathogenic bacteria, especially borrelia spp., rickettsia spp., and anaplasma ... | 2017 | 27922802 |
| bacterial pathogens and endosymbionts in ticks. | ticks collected from goats in northern greece were tested for the presence of tick-borne bacteria. among adult ticks, 37 (57.8%) were rhipicephalus bursa, 11 (17.2%) dermacentor marginatus, 10 (15.6%) ixodes ricinus, 3 (4.7%) rhipicephalus sanguineus sensu lato and 2 (3.1%) haemaphysalis parva; one (1.6%) rhipicephalus spp. tick was nymph. rickettsia monacensis, rickettsia massilae, anaplasma phagocytophilum and anaplasma platys were detected in i. ricinus and rh. bursa ticks. a variety of coxie ... | 2017 | 27686386 |
| molecular detection and identification of rickettsiales pathogens in dog ticks from costa rica. | although vector-borne diseases are globally widespread with considerable impact on animal production and on public health, few reports document their presence in central america. this study focuses on the detection and molecular identification of species belonging to selected bacterial genera (ehrlichia, anaplasma and rickettsia) in ticks sampled from dogs in costa rica by targeting several genes: 16s rrna/dsb genes for ehrlichia; 16s rrna/groel genes for anaplasma, and ompa/glta/groel genes for ... | 2016 | 27480409 |
| molecular detection of anaplasma platys, ehrlichia canis, hepatozoon canis and rickettsia monacensis in dogs from maio island of cape verde archipelago. | tick-borne diseases are emerging worldwide and have an important zoonotic relevance. dogs play an important role in the epidemiology of several zoonotic tick-borne pathogens acting as sentinels and/or reservoirs. this study focused on the molecular identification of tick-borne pathogens in blood samples of 153 autochthonous asymptomatic dogs in maio island, cape verde archipelago. eighty-four (54.9%) dogs were positive for one or more pathogens. fifty-five (35.9%) dogs were infected with hepatoz ... | 2016 | 27177475 |
| first molecular evidence of zoonotic bacteria in ticks in bosnia and herzegovina. | in bosnia and herzegovina, the tick fauna is very diverse, but data on the occurrence of zoonotic tick-borne bacteria are lacking. thus, the aim of this study was to investigate the presence of borrelia burgdorferi sensu lato, anaplasma phagocytophilum, 'candidatus neoehrlichia mikurensis', spotted fever group (sfg) rickettsiae and francisella tularensis in questing ticks. in 19 (21.8%) of 87 ticks (ixodes ricinus, n = 30; dermacentor reticulatus, n = 54; d. marginatus, n = 3) collected by flagg ... | 2016 | 26799474 |
| genetic characterization of candidatus rickettsia vini, a new rickettsia amplified in ticks from la rioja, spain. | a total of 222 ticks removed from birds in la rioja (spain) were screened for spotted fever group rickettsia species using ompa pcr assays. rickettsia monacensis (n=1) and r. sibirica (n=1) were detected. apart from that, 27 out of 29 ixodes spp. dna extracts that tested positive for ompa did not match with any validated spotted fever group rickettsia. multilocus sequence typing for 16s rrna, glta, ompb, sca4, and 17-kda antigen genes was performed, and r. heilongjiangensis was found to be the n ... | 2012 | 23140892 |
| infection of immature ixodes scapularis (acari: ixodidae) by membrane feeding. | a reduction in the use of animals in infectious disease research is desirable for animal welfare as well as for simplification and standardization of experiments. an artificial silicone-based membrane-feeding system was adapted for complete engorgement of adult and nymphal ixodes scapularis say (acari: ixodidae), and for infecting nymphs with pathogenic, tick-borne bacteria. six wild-type and genetically transformed strains of four species of bacteria were inoculated into sterile bovine blood an ... | 2016 | 26721866 |
| molecular detection of emerging tick-borne pathogens in vojvodina, serbia. | ticks play an important role in disease transmission globally due to their capability to serve as vectors for human and animal pathogens. the republic of serbia is an endemic area for a large number of tick-borne diseases. however, current knowledge on these diseases in serbia is limited. the aim of this study was to investigate the presence of new emerging tick-borne pathogens in ticks collected from dogs and the vegetation from different parts of vojvodina, serbia. a total of 187 ticks, includ ... | 2016 | 26565929 |
| tick-borne pathogens in ticks collected from breeding and migratory birds in switzerland. | from 2007 to 2010, 4558 migrating and breeding birds of 71 species were caught and examined for ticks in switzerland. a total of 1205 specimens were collected; all were ixodes ricinus ticks except one ixodes frontalis female, which was found on a common chaffinch (fringilla coelebs) for the first time in switzerland. each tick was analysed individually for the presence of borrelia spp., rickettsia spp., anaplasma phagocytophilum and tick-borne encephalitis virus (tbev). altogether, 11.4% of bird ... | 2014 | 25113989 |
| rickettsiae in questing ixodes ricinus ticks in the czech republic. | tick-borne rickettsiae are an important topic in the field of emerging infectious diseases. in the study, we screened a total of 1473 field-collected ixodes ricinus ticks (1294 nymphs, 99 males, and 80 females) for the presence of human pathogenic rickettsiae (rickettsia helvetica, r. monacensis, 'candidatus neoehrlichia mikurensis', and anaplasma phagocytophilum) in natural and urban ecosystems using molecular techniques. the minimum infection rate (mir) for rickettsia spp. was found to be 2.9% ... | 2014 | 24252265 |
| molecular evidence for bacterial and protozoan pathogens in hard ticks from romania. | the aim of the present study was to provide a preliminary insight into the diversity of tick-borne pathogens circulating at the domestic host-tick interface in romania. for this, feeding and questing ticks were analyzed by real-time polymerase chain reaction (pcr) for the presence of anaplasma phagocytophilum, anaplasma platys, ehrlichia canis, borrelia burgdorferi sensu latu, and by pcr and subsequent sequencing for rickettsia spp., babesia spp. and theileria spp. a total of 382 ticks, encompas ... | 2013 | 23428204 |
| sympatric occurrence of ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks and rickettsia and babesia species in slovakia. | vojka nad dunajom in the south-west of the slovak republic is a locality with sympatric occurrence of 3 species of ticks. this study investigated the spatial distribution of dermacentor reticulatus, ixodes ricinus, and haemaphysalis concinna ticks in this area and determined the prevalence of babesia and rickettsia species in questing adults of these tick species considered as potential risk for humans and animals. ticks were collected by blanket dragging over the vegetation from september 2011 ... | 2014 | 24973275 |
| serological and molecular evidence for spotted fever group rickettsia and borrelia burgdorferi sensu lato co-infections in the netherlands. | only a few reported cases indicate that rickettsia helvetica and rickettsia monacensis can cause disease in humans. exposure to these two spotted fever group (sfg) rickettsiae occurs through bites of ixodes ricinus, also the primary vector of lyme borreliosis in europe. to date, it is unclear how often exposure to these two microorganisms results in infection or disease. we show that of all the borrelia burgdorferi s.l.-positive ticks, 25% were co-infected with rickettsiae. predominantly r. helv ... | 2016 | 26739030 |
| molecular detection and characterization of spotted fever group rickettsiae in ticks from central italy. | the aim of this study was to investigate the presence of rickettsial pathogens in ticks from central italy. a total of 113 ticks hailed from latium and tuscany regions were identified and tested by pcr to detect glta, ompa, ompb genes of rickettsia. positive amplicons were sequenced and identified at species level. ticks were analyzed individually or in pools. the percentage of positivity for sfg rickettsiae was 12.4%, expressed as minimum infection rate (mir) assuming that one tick was positive ... | 2016 | 27365155 |
| rickettsioses in europe. | bacteria of the genera rickettsia and orientia (family rickettsiaceae, order rickettsiales) cause rickettsioses worldwide, and are transmitted by lice, fleas, ticks and mites. in europe, only rickettsia spp. cause rickettsioses. with improvement of hygiene, the risk of louse-borne rickettsiosis (epidemic typhus) is low in europe. nevertheless, recrudescent form of rickettsia prowazekii infection persists. there could be an epidemic typhus outbreak if a body lice epidemic occurs under unfavorable ... | 2016 | 26384814 |
| tick-borne rickettsioses in europe. | rickettsioses are caused by obligate intracellular bacteria within the genus rickettsia, mainly transmitted by arthropods. until recently, mediterranean spotted fever (msf) caused by rickettsia conorii was considered the only tick-borne rickettsiosis in europe. however, 'new' tbr have been described in europe during last years. for instance, other subspecies such as r. conorii caspia and r. conorii israelensis have been involved in msf. dermacentor-borne necrosis erythema and lymphadenopathy/tic ... | 2012 | 23177355 |
| microorganisms in ticks (acari: ixodidae) collected on marsupials and rodents from santa catarina, paraná and mato grosso do sul states, brazil. | information about tick fauna and monitoring of pathogen prevalences in ticks (acari: ixodidae) in various habitat types can enhance knowledge about the epidemiology of tick-borne pathogens in brazil. this work shows the results of a study of tick parasitism of wild rodents and marsupials collected in seven localities in the southern part of brazil, within atlantic forest and cerrado biomes. a total of 61 ticks were collected from small mammals, and after identification to the species level, the ... | 2017 | 27769655 |
| molecular detection of rickettsia species in amblyomma ticks collected from snakes in thailand. | some reptile ticks are potential vectors of pathogens such as spotted fever group (sfg) rickettsiae. here, we report for the first time in detail the molecular evidence, dna sequences and phylogenetic studies, for the presence of rickettsia spp. in amblyomma ticks (amblyomma helvolum and amblyomma varanense) from snakes in thailand. a total of 24 tick samples was collected from 4 snake species and identified. a phylogenetic analysis inferred from the partial sequences of the glta gene indicated ... | 2014 | 25027232 |
| the role of particular tick developmental stages in the circulation of tick-borne pathogens affecting humans in central europe. 3. rickettsiae. | ixodes ricinus, dermacentor reticulatus and d. marginatus ticks are the most important vector for rickettsia spp. in central europe. ticks sustain rickettsial transmission cycles transovarially and transstadially, it makes enable the rickettsial circulation in the tick population in the absence of vertebrate competent reservoir. rickettsia helvetica is transmitted by i. ricinus tick; the highest rates of infection are noted in adult females, lower in males and in nymphs. all tick developmental s ... | 2017 | 27614472 |
| prevalence and diversity of human pathogenic rickettsiae in urban versus rural habitats, hungary. | tick-borne rickettsioses belong to the important emerging infectious diseases worldwide. we investigated the potential human exposure to rickettsiae by determining their presence in questing ticks collected in an urban park of budapest and a popular hunting and recreational forest area in southern hungary. differences were found in the infectious risk between the two habitats. rickettsia monacensis and rickettsia helvetica were identified with sequencing in questing ixodes ricinus, the only tick ... | 2016 | 26613759 |
| spotted fever group rickettsiae detected in immature stages of ticks parasitizing on iberian endemic lizard lacerta schreiberi bedriaga, 1878. | spotted fever rickettsioses are tick-borne diseases of growing public health concern. the prevalence of rickettsia-infected ticks and their ability to parasitize humans significantly influence the risk of human infection. altogether 466 ixodes ricinus ticks (428 nymphs and 38 larvae) collected from 73 lacerta schreiberi lizards were examined by pcr targeting the citrate synthetase gene glta for the presence of rickettsia spp. rickettsial dna was detected in 47% of nymphs and 31.6% of larvae. the ... | 2015 | 26141751 |
| seasonal analysis of rickettsia species in ticks in an agricultural site of slovakia. | many rickettsiae of the spotted fever group are emerging pathogens causing serious diseases associated with vertebrate hosts. ixodidae ticks are known as their vectors. investigation of the relative abundance of questing ixodes ricinus and their infection with rickettsia spp. in an agricultural site comprising a game reserve in slovakia was the aim of this study. in total, 2198 i. ricinus (492 larvae, 1503 nymphs and 203 adults) were collected by flagging the vegetation along 100 m(2) transects ... | 2016 | 26119003 |
| detection and identification of rickettsia species in ixodes tick populations from estonia. | a total of 1640 ticks collected in different geographical parts of estonia were screened for the presence of rickettsia species dna by real-time pcr. dna of rickettsia was detected in 83 out of 1640 questing ticks with an overall prevalence of 5.1%. the majority of the ticks infected by rickettsiae were ixodes ricinus (74 of 83), while 9 of the 83 positive ticks were ixodes persulcatus. for rickettsial species identification, a part of the citrate synthase glta gene was sequenced. the majority o ... | 2015 | 26096853 |
| first detections of rickettsia helvetica and r. monacensis in ectoparasitic mites (laelapidae and trombiculidae) infesting rodents in south-western slovakia. | information on circulation of rickettsiae between small mammals and their ectoparasitic mites is scarce. in this study, we investigated infestation rates of rodents with mites in some areas of sw slovakia and the role of mites as possible vectors of pathogenic rickettsiae. a total of 615 rodents of five species were caught during 2010-2012. all individuals were examined for ectoparasites which resulted in 2821 mites belonging to three species of laelapidae and six species of trombiculidae. the m ... | 2015 | 25843569 |
| rickettsial infection in ixodes ricinus ticks in urban and natural habitats of slovakia. | a total of 1810 ixodes ricinus ticks was collected from the vegetation from 2 different habitat types: urban and natural. urban habitats were represented by cemeteries and public parks in the following towns: bratislava, malacky, and martin at 150 m and 400 m above sea level. natural habitats were selected in the mountain forest of the martinské hole mts. in central slovakia at 3 different altitudinal levels, i.e. 600 m, 800 m and 1000 ma.s.l. all ticks were tested for the presence of spotted fe ... | 2014 | 24342052 |
| use of dna sequences for rickettsia identification in ixodes ricinus ticks: the first detection of rickettsia monacensis in poland. | the cosmopolitan tick ixodes ricinus inhabiting europe, including poland, is a vector for many pathogens, such as various rickettsia species, which spread to new territories. they are present mainly in the mediterranean countries, but have also been found in central europe at increasing frequency. in the present study, the glta gene, encoding citrate synthase, and an internal transcribed spacer (its) were employed to detect the dna and identify the species of tick-borne pathogens of the ricketts ... | 2013 | 23178758 |
| detection of rickettsia monacensis from ixodes nipponensis collected from rodents in gyeonggi and gangwon provinces, republic of korea. | a total of 1,305 ticks were collected from wild rodents captured monthly, except july and august, during 2008 at three us-rok operated military training sites and three us military installations in gyeonggi and gangwon provinces, the republic of korea (rok). ixodes nipponensis was the most frequently collected tick (n = 1,299, 99.5 %), followed by ixodes pomerantzevi (n = 6, 0.5 %). the ticks were pooled (1-15/sample) and tested by nested polymerase chain reaction (npcr) for spotted fever group ... | 2013 | 23624621 |
| spotted fever group rickettsia closely related to rickettsia monacensis isolated from ticks in south jeolla province, korea. | rickettsia monacensis, a spotted fever group rickettsia, was isolated from ixodes nipponensis ticks collected from live-captured small mammals in south jeolla province, korea in 2006. homogenates of tick tissues were inoculated into l929 and vero cell monolayers using shell vial assays. after several passages, giemsa staining revealed rickettsia-like organisms in the inoculated vero cells, but not the l929 cells. sequencing analysis revealed that the ompa-small part (25-614 bp region), ompa-larg ... | 2013 | 23621111 |
| first isolation of rickettsia monacensis from a patient in south korea. | a rickettsia sp. was isolated from the blood of a patient with an acute febrile illness using the shell vial technique; the isolate was named cn45kr and was identified by molecular assay as rickettsia monacensis, which was first recognized as a pathogen in spain. sequencing analysis showed that the glta sequence of the isolate was identical to that of rickettsia sp. irs3. the ompa-5mp fragment sequence showed 100% identity to those of r. monacensis and rickettsia sp. in56 and ompa-3pa in56 and 1 ... | 2017 | 28593634 |
| etiological [corrected] agents of rickettsiosis and anaplasmosis in ticks collected in emilia-romagna region (italy) during 2008 and 2009. | ticks are the main vectors of rickettsiae of the spotted fever group, as well as of a variety of other rickettsiales, including bacteria of the genus anaplasma, that might cause diseases in humans and animals. here we present the result of a survey for ticks and for tick-associated rickettsiales in the emilia romagna region (northern italy). the study was focused on ticks collected from wild-hunted animals. out of 392 ticks collected from these animals, 282 (72%) were identified as ixodes ricinu ... | 2012 | 22402940 |
| molecular detection of rickettsia conorii and other zoonotic spotted fever group rickettsiae in ticks, romania. | the diverse tick fauna as well as the abundance of tick populations in romania represent potential risks for both human and animal health. spotted fever group (sfg) rickettsiae are recognized as important agents of emerging human tick-borne diseases worldwide. however, the epidemiology of rickettsial diseases has been poorly investigated in romania. in urban habitats, companion animals which are frequently exposed to tick infestation, play a role in maintenance of tick populations and as reservo ... | 2016 | 26507182 |