Publications

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utilisation by sheep of herbage under tree crops in ghana.a study conducted into the utilisation by sheep of herbage under a mango/cashew plantation at kade (ghana) showed the native herb, asystasia gangetica to be the most preferred herbage. centrosema pubescens was preferred to pueraria phaseoloides. the physical condition of the herbage affected their preference but the crude protein content did not have any influence. the mean daily digestible dry matter intake of yearling rams (g/kg w0.75) was 34, 31 and 39 for animals on brachiaria lata/pueraria ...1978625790
genetic transformation of pueraria phaseoloides with agrobacterium rhizogenes and puerarin production in hairy roots.an efficient transformation system for the medicinal plant pueraria phaseoloides was established by using agropine-type agrobacterium rhizogenes atcc15834. hairy roots could be obtained directly from the cut edges of petioles of leaf explants or via callus 10 days after inoculation with the bacteria. the highest frequency of explant transformation by a. rhizogenes atcc15834 was about 70% after infection for 30 days. hairy roots could grow rapidly on solid, growth regulator-free murashige and sko ...200312836005
production and economic potentials of cattle in pasture-based systems of the western amazon region of brazil.our objectives were to evaluate strategies to improve productivity and economic returns from beef and dual-purpose cattle systems based on data collected on one dual-purpose (bos taurus x bos indicus) and two beef (nellore) cattle farms in the western amazon region of brazil. forage chemical composition and digestion rates of carbohydrate fractions of grazed brachiaria decumbens and brachiaria brizantha cv. marandu grasses and pueraria phaseoloides (tropical kudzu) legume were measured monthly d ...200314677847
[culture of hairy roots in pueraria phaseoloides and its puerarin production].an efficient transformation system for genetic transformation of medicinal plant, pueraria phaseoloides was developed, by using agropine-type agrobacterium rhizogenes atcc15834. hairy roots could be obtained via callus from the cut edges of leaf explants of p. phaseoloides 20 days after inoculation with agrobacterium. 35 days after infection, the percentage of rooted leaf explants was about 85%. hairy roots could have an autonomous growth on solid or liquid growth regulator-free ms medium but gr ...200314724930
scale-up production of puerarin from hairy roots of pueraria phaseoloides in an airlift bioreactor.hairy roots of the chinese herb, pueraria phaseoloides, obtained from leaf explants and transformed with the agrobacterium rhizogenes, were cultured in 2.5 l airlift bioreactors for three weeks. puerarin accumulated at 5,570 microg g(-1) dry wt, which is near 200 times as much as in 250 ml flask cultures. in addition, puearin was exuded into the nutrient medium at final concentrations higher than in the hairy roots themselves.200415218379
[induction of hairy roots of pueraria phaseoloides and its culture in liquid and solid medium].an efficient transformation system for genetic transformation of medicinal plant, pueraria phaseoloides, which contains puerarin and daidzein with hypothermic, spasmolytic, hypotensive and anti-arrhythmic activities, by using agropine-type agrobacterium rhizogenes atcc 15834 was developed. hairy roots could be obtained directly from the cut edges of petioles of leaf explants of p. phaseoloides or via callus 20 days after inoculation with agrobacterium. the percentage of rooted leaf explants 35 d ...200315969012
symbiotic n 2 -fixation estimated by the (15) n tracer technique and growth of pueraria phaseoloides (roxb.) benth. inoculated with bradyrhizobium strain in field conditions.this field experiment was established in eastern cameroon to examine the effect of selected rhizobial inoculation on n2-fixation and growth of pueraria phaseoloides. treatments consisted of noninoculated and bradyrhizobium yuanmingense s3-4-inoculated pueraria with three replications each. ipomoea batatas as a non-n2-fixing reference was interspersed in each pueraria plot. all the twelve plots received 2 gn/m(2) of (15)n ammonium sulfate 10% atom excess. at harvest, dry matter yields and the nit ...201626904363
sward characteristics and performance of dairy cows in organic grass-legume pastures shaded by tropical trees.the silvopastoral system (sps) has been suggested to ensure sustainability in animal production systems in tropical ecosystems. the objective of this study was to evaluate pasture characteristics, herbage intake, grazing activity and milk yield of holstein×zebu cows managed in two grazing systems (treatments): sps dominated by a graminaceous forage (brachiaria decumbens) intercropped with different leguminous herbaceous forages (stylosanthes spp., pueraria phaseoloides and calopogonium mucunoide ...201424703358
suitability of pueraria phaseoloides, chromolaena odorata and tithonia diversifolia as in-situ mulch for nematode management in musa cropping systems.mulching with plant organic matter has been shown to reduce nematode population densities in various cropping systems. the level of nematode control is increased when such mulches are incorporated into the soil as organic amendments. chromolaena odorata, tithonia diversifolia and pueraria phaseoloides are common cover crops in west and central africa that produce large quantities of nutrient rich biomass. the aim of this study was to determine, if in-situ mulching of c. odorata, t. diversifolia ...200617390809
soil oribatid mite communities under three species of legumes in an ultisol in brazil.oribatid mite densities in the topsoil and their activity at the soil surface were monitored under three species of perennial legume cover crops namely, arachis pintoi, macroptilium atropupureum and pueraria phaseoloides, grass (panicum maximum) and bare plots on three occasions in 1998 and 1999 in a derived savanna zone in brazil. both densities and activity at the soil surface were higher in the early but cool dry season in april 1998 than in the early wet but warm season in november 1998 and ...200212797403
[effect of sucrose concentration on the growth and production of secondary metabolites in pueraria phaseoloides hairy roots].effects of sucrose concentrations on the growth and production of puerarin and isoflavones compounds in pueraria phaseoloides hairy roots induced by agrobacterium rhizogenes atcc15834 were investigated. changes of sucrose consumption in the medium during liquid culture were also determined. the results showed cultured for 16 days in ms medium with 5%, 4%, 3% and 2% sucrose, the proliferation times of dry weight of hairy roots were 11.7, 11.9, 10.1 and 5.9, respectively. 3% sucrose concentration ...200415636366
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