| highly polymorphic microsatellite loci in the heron reef population of the tropical abalone haliotis asinina. | | 2000 | 10964245 |
| mox homeobox expression in muscle lineage of the gastropod haliotis asinina: evidence for a conserved role in bilaterian myogenesis. | mox homeobox genes are expressed during early vertebrate somitogenesis. here we describe the expression of has-mox, a mox gene from the gastropod haliotis asinina. has-moxis expressed in the trochophore larva in paraxial mesodermal bands. during larval development, has-mox expression remains restricted to mesodermal cells destined to form adult muscle in the foot. this restricted expression of has-mox in haliotis is similar to that observed for vertebrate mox genes, suggesting a conserved role i ... | 2002 | 11976952 |
| ecological regulation of development: induction of marine invertebrate metamorphosis. | in the marine environment a wide range of invertebrates have a pelagobenthic lifecycle that includes planktonic larval and benthic adult phases. transition between these morphologically and ecologically distinct phases typically occurs when the developmentally competent larva comes into contact with a species-specific environmental cue. this cue acts as a morphogenetic signal that induces the completion of the postlarval/juvenile/adult developmental program at metamorphosis. the development of c ... | 2002 | 12141457 |
| pleiotropic developmental expression of haspou-iii, a class iii pou gene, in the gastropod haliotis asinina. | haspou-iii is expressed in multiple cell types during the first 3 days of development of the gastropod haliotis asinina. haspou-iii expression begins in two bilaterally symmetrical sets of cells on the ventral ectodermal surface of the trochophore larva; one set are putative foot mucous cells. after torsion, haspou-iii transcripts transiently appear in the developing ganglia of the central nervous system. at the end of larval morphogenesis, haspou-iii expression is initiated in dorsoposterior ce ... | 2002 | 12175498 |
| developmental expression of a class iv pou gene in the gastropod haliotis asinina supports a conserved role in sensory cell development in bilaterians. | pou-iv genes regulate neuronal development in a number of deuterostomes (chordates) and ecdysozoans (arthropods and nematodes). currently their function and expression in the third bilaterian clade, the lophotrochozoa, comprising molluscs, annelids and their affiliates, is unclear. herein we characterise the developmental expression of haspou-iv in the gastropod mollusc, haliotis asinina. the pou-iv gene is transiently expressed in 11 distinct larval territories during the first 3 days of develo ... | 2002 | 12203096 |
| genetic diversity and molecular markers of the tropical abalone (haliotis asinina) in thailand. | genetic diversity of abalone in thailand, haliotis asinina, h. ovina, and h. varia, was analyzed by polymerase chain reaction (pcr) of 18s and 16s rdnas, with randomly amplified polymorphic dna (rapd) and restriction fragment length polymorphism (rflp). species-specific rapd markers were found in each abalone species. restriction analysis of 18s (nuclear) ribosomal dna with alu i, taq i, and hae iii and 16s (mitochondrial) rdna with bam hi, eco ri, hae iii, and alu i gave 12 and 13 digestion pat ... | 2003 | 12913813 |
| expression of anterior hox genes during larval development of the gastropod haliotis asinina. | we report the spatial expression patterns of five anterior hox genes during larval development of the gastropod mollusc haliotis asinina, an unsegmented spiralian lophotrochozoan. molecular alignments and phylogenetic analysis indicate that these genes are homologues of drosophila hom-c genes labial, proboscipedia, zen, deformed, and sex combs reduced; the abalone genes are named has-hox1, -hox2, -hox3, -hox4, and -hox5. has-hox transcripts are first detected in the free-swimming trochophore lar ... | 2003 | 12950629 |
| expression of pou, sox, and pax genes in the brain ganglia of the tropical abalone haliotis asinina. | in gastropod mollusks, neuroendocrine cells in the anterior ganglia have been shown to regulate growth and reproduction. as a first step toward understanding the molecular mechanisms underlying the regulation of these physiological processes in the tropical abalone haliotis asinina, we have identified sets of pou, sox, and pax transcription factor genes that are expressed in these ganglia. using highly degenerate oligonucleotide primers designed to anneal to conserved codons in each of these gen ... | 2000 | 14961178 |
| microsatellite genotyping of individual abalone larvae: parentage assignment in aquaculture. | in aquaculture, microsatellite dna markers are used to genotype parental broodstock, to assess fertilization success, and to maintain pedigree information for selective breeding. in this study we genotyped individual haliotis asinina larvae by analyzing a suit of polymorphic microsatellite loci. at least 10 loci can be analyzed from a single abalone veliger larva. we assayed 5 polymorphic loci to identify the parents of individual larvae produced in 3 separate crosses. in all cases, the parents ... | 2001 | 14961341 |
| expression of pax258 in the gastropod statocyst: insights into the antiquity of metazoan geosensory organs. | most animals have sensory systems that allow them to balance and orient relative to the pull of gravity. structures responsible for these functions range from very simple statocysts found in many aquatic invertebrates to the complex inner ear of mammals. previous studies suggest that the specialized mechanosensory structures responsible for balance in vertebrates and insects may be homologous based on the requirement and expression of group ii pax genes (i.e., pax-2/5/8 genes). here we report th ... | 2003 | 14984039 |
| species identification of the tropical abalone (haliotis asinina, haliotis ovina, and haliotis varia) in thailand using rapd and scar markers. | a randomly amplified polymorphic dna (rapd) analysis was used to identify the species- and population-specific markers of abalone; haliotis asinina, h. ovina, and h. varia in thai waters. fifteen species-specific and six population-specific rapd markers were identified. in addition, an 1650 bp band (ubc195) that was restricted to h. ovina from the gulf of thailand (east) was also found. all of the specific rapd markers were cloned and sequenced. twenty pairs of primers were designed and specific ... | 2004 | 15469698 |
| basic nuclear protein pattern and chromatin condensation in the male germ cells of a tropical abalone, haliotis asinina. | the basic nuclear proteins (bnps) in spermatozoa of a tropical abalone, haliotis asinina, were composed of a majority of protamine-like (pl) protein and a small amount of histones h1 and h4. abalone h1 and pl proteins exhibited strong immunological cross reactivities among themselves as well as with chick h5 and calf thymus h1. thus, all these proteins may belong to the same family. immunolocalization by indirect immunofluorescence and immunoelectron microscopy indicated that h1 and h4 were pres ... | 2005 | 15570617 |
| population structure of tropical abalone (haliotis asinina) in coastal waters of thailand determined using microsatellite markers. | three partial genomic libraries were constructed from genomic dna of the tropical abalone (haliotis asinina) that was digested with alui, vortexed/sonicated, and digested with mixed enzyme (alui, hincii, and rsai). the libraries yielded 0.02%, 0.42%, and 1.46% positive microsatellite-containing clones, respectively. eleven clones each of perfect, imperfect, and compound microsatellites were isolated. ten primer pairs (cuhas1-cuhas10) were analyzed to evaluate their polymorphic level. the numbers ... | 2004 | 15747095 |
| egg-laying-hormone immunoreactivity in the neural ganglia and ovary of haliotis asinina linnaeus. | immunoreactivity against the abalone egg-laying hormone (aelh) was detected in the fine granules of type 1 and 2 neurosecretory (ns) cells, neurites in the neuropil, and blood sinuses in the connective tissue sheath of the cerebral, pleuropedal, and visceral ganglia of the tropical abalone, haliotis asinina linnaeus. the number of positive ns cells, and the intensity of staining in the ganglia, varied and might be related to the stage of ovarian cycle. at any stage, positive cells were most nume ... | 2005 | 15803317 |
| genetic heterogeneity of the tropical abalone (haliotis asinina) revealed by rapd and microsatellite analyses. | genetic heterogeneity of the tropical abalone, haliotis asinina was examined using randomly amplified polymorphic dna (rapd) and microsatellite analyses. one hundred and thirteen polymorphic rapd fragments were generated. the percentage of polymorphic bands of h. asinina across overall primers was 85.20%. the average genetic distance of natural samples within the gulf of thailand (hacame and hasame) was 0.0219. larger distance was observed when those samples were compared with hatraw from the an ... | 2005 | 15826495 |
| developmental expression of two haliotis asinina hemocyanin isoforms. | hemocyanins are large copper-containing respiratory proteins that play a role in oxygen transport in many molluscs. in some species only one hemocyanin isoform is present while in others two are expressed. the physiological relevance of these isoforms is unclear and the developmental and tissue-specific expression of hemocyanin genes is largely unknown. here we show that two hemocyanin genes in the gastropod haliotis asinina, which encode h. asinina hemocyanin (hah1) and hah2 isoforms, are devel ... | 2005 | 16219038 |
| parasitic castration by the digenian trematode allopodocotyle sp. alters gene expression in the brain of the host mollusc haliotis asinina. | infection of molluscs by digenean trematode parasites typically results in the repression of reproduction -- the so-called parasitic castration. this is known to occur by altering the expression of a range of host neuropeptide genes. here we analyse the expression levels of 10 members of pou, pax, sox and hox transcription factor gene families, along with genes encoding fmrfamide, prohormone convertase and beta-tubulin, in the brain ganglia of actively reproducing (summer), non-reproducing (wint ... | 2006 | 16777099 |
| a rapidly evolving secretome builds and patterns a sea shell. | instructions to fabricate mineralized structures with distinct nanoscale architectures, such as seashells and coral and vertebrate skeletons, are encoded in the genomes of a wide variety of animals. in mollusks, the mantle is responsible for the extracellular production of the shell, directing the ordered biomineralization of caco3 and the deposition of architectural and color patterns. the evolutionary origins of the ability to synthesize calcified structures across various metazoan taxa remain ... | 2006 | 17121673 |
| pleistocene isolation and recent gene flow in haliotis asinina, an indo-pacific vetigastropod with limited dispersal capacity. | haliotis asinina is a broadcast-spawning mollusc that inhabits indo-pacific coral reefs. this tropical abalone develops through a nonfeeding larval stage that is competent to settle on specific species of coralline algae after 3-4 days in the plankton. failure to contact an inductive algae within 10 days of hatching usually results in death. these life cycle characteristics suggest a limited capacity for dispersal and thus gene flow. this makes h. asinina particularly suitable for elucidating ph ... | 2007 | 17217345 |
| developmental expression of hsp90, hsp70 and hsf during morphogenesis in the vetigastropod haliotis asinina. | heat shock proteins (hsps) have dual functions, participating in both the stress response and a broad range of developmental processes. at physiological temperatures, it has been demonstrated in deuterostomes (vertebrates) and ecdysozoans (insects) that hsps are expressed in tissues that are undergoing differentiation and morphogenesis. here we investigate the developmental expression of hsp70, hsp90 and their regulatory transcription factor heat shock transcription factor (hsf) in the marine ga ... | 2007 | 17647016 |
| dynamic expression of ancient and novel molluscan shell genes during ecological transitions. | the mollusca constitute one of the most morphologically and ecologically diverse metazoan phyla, occupying a wide range of marine, terrestrial and freshwater habitats. the evolutionary success of the molluscs can in part be attributed to the evolvability of the external shell. typically, the shell first forms during embryonic and larval development, changing dramatically in shape, colour and mineralogical composition as development and maturation proceeds. major developmental transitions in shel ... | 2007 | 17845714 |
| the role of mapk signaling in patterning and establishing axial symmetry in the gastropod haliotis asinina. | gastropods are members of the spiralia, a diverse group of invertebrates that share a common early developmental program, which includes spiral cleavage and a larval trochophore stage. the spiral cleavage program results in the division of the embryo into four quadrants. specification of the dorsal (d) quadrant is intimately linked with body plan organization and in equally cleaving gastropods occurs when one of the vegetal macromeres makes contact with overlying micromeres and receives an induc ... | 2007 | 17916345 |
| impact of ecologically relevant heat shocks on hsp developmental function in the vetigastropod haliotis asinina. | heat shock proteins (hsps) are essential for cellular maintenance, normal differentiation and morphogenesis, and protection against a range of environmental stresses. it is unknown which of these roles takes precedence when they are required simultaneously. here we examined the impact of thermal stress on the complex developmental expression patterns of hashsp70 and hashsp90a in the vetigastropod haliotis asinina. we find that near-lethal heat shocks do not alter the spatial demarcation of hsp e ... | 2008 | 18421770 |
| articulated coralline algae of the genus amphiroa are highly effective natural inducers of settlement in the tropical abalone haliotis asinina. | the initiation of metamorphosis in marine invertebrates is strongly linked to the environment. planktonic larvae typically are induced to settle and metamorphose by external cues such as coralline algae (corallinaceae, rhodophyta). although coralline algae are globally abundant, invertebrate larvae of many taxa settle in response to a very limited suite of species. this specificity impacts population structure, as only locations with the appropriate coralline species can attract new recruits. ab ... | 2008 | 18723641 |
| isolation and characterization of testis-specific dmrt1 in the tropical abalone (haliotis asinina). | the doublesex male abnormal-3 related transcription factor-1 (dmrt1) gene encodes a protein containing the dna-binding motif called the dm domain, involved in the sexual development of various species. to gain insight into its implications for gonadal differentiation in the tropical abalone (haliotis asinina), a dmrt1 homolog was identified and characterized. the full length cdna of hadmrt1 (1,740 bp with an orf of 732 bp corresponding to a putative polypeptide of 243 amino acids) and its dm dom ... | 2009 | 19067156 |
| widespread transcriptional changes pre-empt the critical pelagic-benthic transition in the vetigastropod haliotis asinina. | larval settlement is a vital transition period for marine invertebrates and can have far-reaching effects on the ecology and evolution of a species. to explore the molecular mechanisms of this critical process in a nonmodel organism, the abalone haliotis asinina, we employed cdna microarrays. by comparing gene expression profiles through mid- to late larval development and metamorphosis, we identified 144 genes as candidates for a role in competence and/or metamorphosis. gene characterization in ... | 2009 | 19207244 |
| identification, characterization, and expression of the genes tektina1 and axonemal protein 66.0 in the tropical abalone haliotis asinina. | the genes tektin a1 and axonemal protein 66.0 were successfully isolated and characterized in the tropical abalone haliotis asinina. the full-length cdnas of ha-teka1 and ha-axp66.0 were 2166 and 2038 bp long, with orfs of 1350 and 1683 bp, respectively. both ha-teka1 and ha-axp66.0 were expressed in the testes but not in the ovaries or hemocytes of h. asinina adults. in addition, haaxp66.0 was not expressed in h. asinina juveniles (2, 3, and 5 months old). a tissue expression analysis showed ha ... | 2009 | 19583503 |
| expression of prohormone convertase 2 and the generation of neuropeptides in the developing nervous system of the gastropod haliotis. | prohormone convertase 2 (pc2) belongs to a family of enzymes involved in the proteolytic maturation of neuropeptide precursors into mature peptides that act as neurotransmitters, neuromodulators or neurohormones. here we show that a gene encoding a pc2-like enzyme (haspc2) is expressed during larval development and in the adult ganglia of the vetigastropod haliotis asinina. haspc2 exhibits high sequence identity to other gastropod pc2s and thus is likely to function in peptide processing. analys ... | 2009 | 19598126 |
| the existence of gonadotropin-releasing hormone-like peptides in the neural ganglia and ovary of the abalone, haliotis asinina l. | gonadotropin-releasing hormone (gnrh) is a neuropeptide that is conserved in both vertebrate and invertebrate species. in this study, we have demonstrated the presence and distribution of two isoforms of gnrh-like peptides in neural ganglia and ovary of reproductively mature female abalone, haliotis asinina, using immunohistochemistry. we found significant immunoreactivities (ir) of anti-lamprey(l) gnrh-iii and anti-tunicate(t) gnrh, but with variation of labeling intensity by each anti-gnrh typ ... | 2010 | 19604545 |
| settlement specifics: effective induction of abalone settlement and metamorphosis corresponds to biomolecular composition of natural cues. | chemical signaling plays a major role in shaping life history processes that drive ecology and evolution in marine systems, notably including habitat selection by marine invertebrate larvae that must settle out of the plankton onto the benthos.1 for larvae, the identification of appropriate habitats in which to settle and undergo metamorphosis to the adult form relies heavily on the recognition of cues indicative of a favorable environment. by documenting settlement responses of larvae of the tr ... | 2009 | 19721887 |
| carry-over effect of larval settlement cue on postlarval gene expression in the marine gastropod haliotis asinina. | the drastic shift from pelagic larvae to benthic adult form that occurs during marine invertebrate metamorphosis is often induced by intimate interactions between settling larvae and their benthic environment. larval experience prior to and during metamorphosis can significantly affect adult fitness, but it is presently unknown whether the exact nature of the inductive cue is an experience that matters, or by what mechanism such carry-over effects are mediated. here we test for carry-over effect ... | 2009 | 19793199 |
| parallel evolution of nacre building gene sets in molluscs. | the capacity to biomineralize is closely linked to the rapid expansion of animal life during the early cambrian, with many skeletonized phyla first appearing in the fossil record at this time. the appearance of disparate molluscan forms during this period leaves open the possibility that shells evolved independently and in parallel in at least some groups. to test this proposition and gain insight into the evolution of structural genes that contribute to shell fabrication, we compared genes expr ... | 2010 | 19915030 |
| identifying the germline in an equally cleaving mollusc: vasa and nanos expression during embryonic and larval development of the vetigastropod haliotis asinina. | members of the vasa and nanos gene families are important for the specification and development of the germline in diverse animals. here, we determine spatial and temporal expression of vasa and nanos to investigate germline development in the vetigastropod haliotis asinina. this is the first time these genes have been examined in an equally cleaving lophotrochozoan species. we find that hasvasa and hasnanos have largely overlapping, but not identical, expression patterns during embryonic and la ... | 2010 | 20095031 |
| genetic diversity of wild and domesticated stocks of thai abalone, haliotis asinina (haliotidae), analyzed by single-strand conformational polymorphism of aflp-derived markers. | amplified fragment length polymorphism (aflp) analysis was carried out on representative individuals of wild haliotis asinina using 64 primer combinations. nine polymorphic aflps were cloned and sequenced. sequence-specific primers were designed from six aflp-derived fragments. three sequence-characterized amplified region (scar) markers (hascar(320), hascar(295), hascar(327)) were selected for genotyping of 8-month-old domesticated stocks of h. asinina cultured separately at sichang marine scie ... | 2010 | 20568059 |
| proteomic analysis of the organic matrix of the abalone haliotis asinina calcified shell. | abstract: | 2010 | 21050442 |
| developmental expression of coe across the metazoa supports a conserved role in neuronal cell-type specification and mesodermal development. | the transcription factor coe (collier/olfactory-1/early b cell factor) is an unusual basic helix-loop-helix transcription factor as it lacks a basic domain and is maintained as a single copy gene in the genomes of all currently analysed non-vertebrate metazoan genomes. given the unique features of the coe gene, its proposed ancestral role in the specification of chemosensory neurons and the wealth of functional data from vertebrates and drosophila, the evolutionary history of the coe gene can be ... | 2010 | 21069538 |
| molecular analysis of two fmrfamide-encoding transcripts expressed during the development of the tropical abalone haliotis asinina. | fmrfamide-related peptides (farps) are involved in numerous neural functions across the animal kingdom and serve as important models for understanding the evolution of neuropeptides. gastropod molluscs have proved to be particularly useful foci for such studies, but the developmental expression of farps and the evolution of specific transcripts for different peptides are unclear within the molluscs. here we show that farps are encoded by two transcripts that appear to be splice variants of a sin ... | 2011 | 21452226 |
| ultrastructure of the mantle of the gastropod haliotis asinina and mechanisms of shell regionalization. | the ability of a biological system to drive the formation of a microstructure as complex and ordered as the molluscan shell is of immense interest to the fields of nanotechnology and biomedicine. although recent studies have greatly expanded our knowledge of the genes involved in shell formation, the mechanism by which matrix proteins are regulated and directed to the appropriate region of the shell, a process critical for microstructure control, is still obscure. the formation of microstructure ... | 2011 | 21525717 |
| proteomic strategy for identifying mollusc shell proteins using mild chemical degradation and trypsin digestion of insoluble organic shell matrix: a pilot study on haliotis tuberculata. | a successful strategy for the identification of shell proteins is based on proteomic analyses where soluble and insoluble fractions isolated from organic shell matrix are digested with trypsin with the aim of generating peptides, which are used to identify novel shell proteins contained in databases. however, using trypsin as a sole degradative agent is limited by the enzyme's cleavage specificity and is dependent upon the occurrence of lysine and arginine in the shell protein sequence. to bypas ... | 2011 | 22160345 |
| characterization of mucus-associated proteins from abalone (haliotis) - candidates for chemical signaling. | living in groups is a widespread phenomenon in the animal kingdom. for free-spawning aquatic animals, such as the abalone (haliotis), being in the close proximity to potential mating partners enhances reproductive success. in this study, we investigated whether chemical cues could be present in abalone mucus that enable species-specific aggregation. a comparative ms analysis of mucus obtained from trailing or fixed stationary haliotis asinina, and from seawater surrounding aggregations, indicate ... | 2011 | 22129240 |