| production and characterization of a thermostable alpha-amylase from nocardiopsis sp. endophyte of yam bean. | thermostable amylolytic enzymes have been currently investigated to improve industrial processes of starch degradation. studies on production of alpha-amylase by nocardiopsis sp., an endophytic actinomycete isolated from yam bean (pachyrhizus erosus l. urban), showed that higher enzyme levels were obtained at the end of the logarithmic growth phase after incubation for 72 h at ph 8.6. maximum activity of alpha-amylase was obtained at ph 5.0 and 70 degrees c. the isolated enzyme exhibited thermos ... | 2001 | 11131797 |
| ingestion of insoluble dietary fibre increased zinc and iron absorption and restored growth rate and zinc absorption suppressed by dietary phytate in rats. | we examined the effects of ingestion of five types of insoluble fibre on growth and zn absorption in rats fed a marginally zn-deficient diet (6.75 mg (0.103 mmol) zn/kg diet) with or without added sodium phytate (12.6 mmol/kg diet). the types of insoluble fibre tested were corn husks, watermelon skin, yam-bean root (pachyrhizus erosus) and pineapple core, and cellulose was used as a control (100 g/kg diet). body-weight gain in the cellulose groups was suppressed by 57 % by feeding phytate. body- ... | 2001 | 11591231 |
| symbiotic root nodule bacteria isolated from yam bean (pachyrhizus erosus). | a total of 25 isolates from root nodules of yam bean (pachyrhizus erosus l. urban), a tuber-producing leguminous plant, were characterized. all isolates formed effective nodules mainly on lateral roots while edible tubers were developed on the taproot. the root nodules formed were identified as the typical determinate type. by an analysis of the partial sequences of the 16s rrna gene (approximately 300 bp) of 10 strains which were selected randomly, the isolated root nodule bacteria of yam bean ... | 2002 | 12469317 |
| agronomic effectiveness of biofertilizers with phosphate rock, sulphur and acidithiobacillus for yam bean grown on a brazilian tableland acidic soil. | phosphate rocks have low available p and soluble p fertilizers have been preferably used in plant crop production, although economic and effective p sources are needed. experiments were carried out on a brazilian typic fragiudult soil with low available p to evaluate the agronomic effectiveness of phosphate rock (pr) compared with soluble phosphate fertilizer. yam bean (pachyrhizus erosus) inoculated with rhizobia (strains nfb 747 and nfb 748) or not inoculated was the test crop. biofertilizers ... | 2007 | 16815009 |
| isolation, characterization and cloning of a cdna encoding a new antifungal defensin from phaseolus vulgaris l. seeds. | the pvd1 defensin was purified from phaseolus vulgaris (cv. pérola) seeds, basically as described by terras et al. [terras frg, schoofs hme, de bolle mfc, van leuven f, ress sb, vanderleyden j, cammue bpa, broekaer twf. analysis of two novel classes of plant antifungal proteins from radish (raphanus sativus l.) seeds. j biol chem 1992;267(22):15301-9], with some modifications. a deae-sepharose, equilibrated with 20mm tris-hcl, ph 8.0, was initially utilized for the separation of peptides after a ... | 2008 | 18786582 |
| bradyrhizobium pachyrhizi sp. nov. and bradyrhizobium jicamae sp. nov., isolated from effective nodules of pachyrhizus erosus. | several strains isolated from the legume pachyrhizus erosus were characterized on the basis of diverse genetic, phenotypic and symbiotic approaches. these novel strains formed two groups closely related to bradyrhizobium elkanii according to their 16s rrna gene sequences. strains pac48t and pac68t, designated as the type strains of these two groups, presented 99.8 and 99.1% similarity, respectively, in their 16s rrna gene sequences with respect to b. elkanii usda 76t. in spite of these high simi ... | 2009 | 19567584 |
| physico-chemical characteristics and functional properties of chitin and chitosan produced by mucor circinelloides using yam bean as substrate. | microbiological processes were used for chitin and chitosan production by mucor circinelloides (ucp 050) grown in yam bean (pachyrhizus erosus l. urban) medium. the polysaccharides were extracted by alkali-acid treatment and structural investigations by x-ray diffraction, fourier transform ir analysis, viscosity and thermal analysis by tg, dtg, and dta were done. the highest biomass yield (20.7 g/l) was obtained at 96 hours. the highest levels of chitosan (64 mg/g) and chitin (500 mg/g) were pro ... | 2011 | 21862956 |
| genome sequence of bradyrhizobium pachyrhizi strain pac48t, a nitrogen-fixing symbiont of pachyrhizus erosus (l.) urb. | bradyrhizobium pachyrhizi pac48(t) has been isolated from a jicama nodule in costa rica. the draft genome indicates high similarity with that of bradyrhizobium elkanii. several coding sequences (cdss) of the stress response might help in survival in the tropics. pac48(t) carries nodd1 and nodk, similar to bradyrhizobium (parasponia) anu 289 and a particular nodd2 gene. | 2015 | 26383651 |
| influence of bacterial n-acyl-homoserine lactones on growth parameters, pigments, antioxidative capacities and the xenobiotic phase ii detoxification enzymes in barley and yam bean. | bacteria are able to communicate with each other and sense their environment in a population density dependent mechanism known as quorum sensing (qs). n-acyl-homoserine lactones (ahls) are the qs signaling compounds of gram-negative bacteria which are frequent colonizers of rhizospheres. while cross-kingdom signaling and ahl-dependent gene expression in plants has been confirmed, the responses of enzyme activities in the eukaryotic host upon ahls are unknown. since ahl are thought to be used as ... | 2015 | 25914699 |
| interspecies variation of kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media. | an endophytic actinomycete isolated from tubers of yam beam (pachyrhizus erosus l. urban) was classified as a novel species nominated kitasatospora recifensis based in phenotypic and genotypic analysis (16s rdna gene sequence). monosporic culture using specific isp2 media revealed three interspecies, which were identified by dna southern hybridization (wild strain 13817 w, aerial mycelium strain 13817 am and vegetative mycelium strain 13817 vm). the strains were tested for the production of amyl ... | 2007 | 27517827 |
| potential of chitosan from mucor rouxxi ucp064 as alternative natural compound to inhibit listeria monocytogenes. | listeria monocytogenes is widely distributed in nature and the infection listeriosis is recognized as a potential threat for human health because of its mortality rate. the objective of this study was to evaluate the growth profile and chitosan production by mucor rouxxi ucp 064 grown in yam bean (pachyrhizus erosus l. urban) medium. it was also to assess the anti-l. monocytogenes efficacy of the obtained chitosan. higher values of biomass of m. rouxxi (16.9 g.l(-1)) and best yield of chitosan ( ... | 2009 | 24031403 |
| purification and characterization of a novel type i ribosome inactivating protein, pachyerosin, from pachyrhizus erosus seeds, and preparation of its immunotoxin against human hepatoma cells. | pachyrhizus erosus seeds have a high protein content and are used in china due to their cytotoxic effect. here we report the biological and pharmacological activity of the protein extracts from p. erosus seeds. a novel ribosome-inactivating protein, pachyerosin, from p. erosus seeds was successively purified to homogeneity using ammonium sulfate precipitation, deae-sepharose ff, and sephacryl s-200. pachyerosin showed to be a type i ribosome-inactivating protein with a molecular mass of 29 kda a ... | 2014 | 25029173 |
| botanical origin of dietary supplements labeled as "kwao keur", a folk medicine from thailand. | in the course of our study on the quality of dietary supplements in japan, both the internal transcribed spacer (its) sequence of nrdna and the rps16 intron sequence of cpdna of products labeled as "kwao keur" were investigated. as a result, the dna sequence of pueraria candollei var. mirifica, which is the source plant of kwao keur, was observed in only about half of the products. inferred from the determined sequences, source plants in the other products included medicago sativa, glycyrrhiza u ... | 2014 | 23677774 |