| bacterial identification, clinical significance, and antimicrobial susceptibilities of acinetobacter ursingii and acinetobacter schindleri, two frequently misidentified opportunistic pathogens. | the species belonging to the acinetobacter genus are currently reported as opportunistic pathogens in hospitalized patients with underlying predispositions. however, except for the acinetobacter calcoaceticus-acinetobacter baumannii complex, the identification of other species is frequently unreliable, especially for acinetobacter ursingii and acinetobacter schindleri, newly described in 2001. thus, the clinical significance, phenotypic features, and antimicrobial susceptibilities of these two m ... | 2006 | 17050816 |
| nodeomics: pathogen detection in vertebrate lymph nodes using meta-transcriptomics. | the ongoing emergence of human infections originating from wildlife highlights the need for better knowledge of the microbial community in wildlife species where traditional diagnostic approaches are limited. here we evaluate the microbial biota in healthy mule deer (odocoileus hemionus) by analyses of lymph node meta-transcriptomes. cdna libraries from five individuals and two pools of samples were prepared from retropharyngeal lymph node rna enriched for polyadenylated rna and sequenced using ... | 2010 | 20976145 |
| detection of new delhi metallo-β-lactamase (encoded by blandm-1) in acinetobacter schindleri during routine surveillance. | a carbapenem-resistant alcaligenes faecalis strain was isolated from a surveillance swab of a service member injured in afghanistan. the isolate was positive for bla(ndm) by real-time pcr. species identification was reevaluated on three identification systems but was inconclusive. genome sequencing indicated that the closest relative was acinetobacter schindleri and that bla(ndm-1) was carried on a plasmid that shared >99% identity with one identified in an acinetobacter lwoffii isolate. the iso ... | 2013 | 23554204 |
| development and evaluation of species-specific pcr for detection of nine acinetobacter species. | molecular methods have the potential to improve the speed and accuracy of acinetobacter species identification in clinical settings. the goal of this study is to develop species-specific pcr assays based on differences in the rna polymerase beta-subunit gene (rpob) to detect nine commonly isolated acinetobacter species including acinetobacter baumannii, acinetobacter calcoaceticus, acinetobacter pittii, acinetobacter nosocomialis, acinetobacter lwoffii, acinetobacter ursingii, acinetobacter bere ... | 2016 | 27312551 |
| the effect of temperature on antibacterial activity of biosynthesized silver nanoparticles. | the purpose of this study was the evaluation of two different temperatures on antibacterial activity of the biosynthesized silver nanoparticles. 38 silver nanoparticles-producing bacteria were isolated from soil and identified. biosynthesis of silver nanoparticles by these bacteria was verified through visible light spectrophotometry. two strains were relatively active for production of silver nanoparticles. these strains were subjected for molecular identification and recognized as bacillus sp. ... | 2013 | 23324852 |
| host plant induced variation in gut bacteria of helicoverpa armigera. | helicoverpa are important polyphagous agricultural insect pests and they have a worldwide distribution. in this study, we report the bacterial community structure in the midgut of fifth instar larvae of helicoverpa armigera, a species prevalent in the india, china, south asia, south east asia, southern & eastern africa and australia. using culturable techniques, we isolated and identified members of bacillus firmus, bacillus niabense, paenibacillus jamilae, cellulomonas variformis, acinetobacter ... | 2012 | 22292034 |
| multiplex pcr to detect the genes encoding naturally occurring oxacillinases in acinetobacter spp. | bacteria of the genus acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. species identification is difficult and there is a need for simple molecular methods to differentiate between the species. naturally occurring oxacillinase genes (blaoxa) have been identified in several acinetobacter species and their detection by pcr can aid in species identification. the aim of this study was to develop a multiplex pcr to identify intrinsic blao ... | 2014 | 24288029 |
| acinetobacter ursingii sp. nov. and acinetobacter schindleri sp. nov., isolated from human clinical specimens. | the taxonomic status of two recently described phenetically distinctive groups within the genus acinetobacter, designated phenon 1 and phenon 2, was investigated further. the study collection included 51 strains, mainly of clinical origin, from different european countries with properties of either phenon 1 (29 strains) or phenon 2 (22 strains). dna-dna hybridization studies and dna polymorphism analysis by aflp revealed that these phenons represented two new genomic species. furthermore, 16s rr ... | 2001 | 11594623 |
| genomic and physiological characterization of a laboratory-isolated acinetobacter schindleri ace strain that quickly and efficiently catabolizes acetate. | an acinetobacter strain, designated ace, was isolated in the laboratory. phylogenetic tests and average nucleotide identity value comparisons suggested that ace belongs to the species acinetobacterschindleri. we report for the first time the complete genome sequence of an a. schindleri strain, which consists of a single circular chromosome of 3 001 209 bp with an overall dna g+c content of 42.9 mol% and six plasmids that account for 266 844 bp of extrachromosomal material. the presence or absenc ... | 2017 | 28671531 |
| molecular characterization of microbial communities in bioaerosols of a coal mine by 454 pyrosequencing and real-time pcr. | microbial diversity and abundance in bioaerosols of a coal mine were analyzed based on 454 pyrosequencing and real-time polymerase chain reaction (pcr). a total of 37,191 high quality sequences were obtained and could be classified into 531, 1730 and 448 operational taxonomic units respectively for archaea, bacteria and fungi at 97% sequence similarity. the shannon diversity index for archaea, bacteria and fungi was respectively 4.71, 6.29 and 3.86, indicating a high diversity in coal mine bioae ... | 2015 | 25872733 |
| acinetobacter gandensis sp. nov. isolated from horse and cattle. | we previously reported the presence of an oxa-23 carbapenemase in an undescribed species of the genus acinetobacter isolated from horse dung at the faculty of veterinary medicine, ghent university, belgium. here we include six strains to corroborate the delineation of this taxon by phenotypic characterization, dna-dna hybridization, 16s rrna gene and rpob sequence analysis, % g+c determination, maldi-tof ms and fatty acid analysis. the nearly complete 16s rrna gene sequence of strain ug 60467(t) ... | 2014 | 25225259 |