| biotransformation of the trichoderma metabolite 6-n-pentyl-2h-pyran-2-one (6pap) by selected fungal isolates | a variety of fungi were tested for their ability to transform the antifungal trichoderma metabolite 6-n-pentyl-2h-pyran-2-one (6pap) (1). three penicillium isolates, a sclerotinia isolate, and a fusarium isolate were all able to rapidly metabolize 1 and gave mixtures of isomers of monohydroxylated 1 and, in some cases, products resulting from further oxidation to carboxylic acids. among these products were four previously unidentified metabolites (6, 7, 8, and 9) which were isolated and characte ... | 1999 | 10346944 |
| functional expression of the gene cu, encoding the phytotoxic hydrophobin cerato-ulmin, enables ophiostoma quercus, a nonpathogen on elm, to cause symptoms of dutch elm disease. | we studied the involvement of the phytotoxic hydrophobin cerato-ulmin (cu) in pathogenesis and virulence of dutch elm disease (ded) by expressing its encoding gene (cu) in ophiostoma quercus, a nonpathogenic species on elm closely related to the ded pathogens o. ulmi and o. novo-ulmi. the production of the toxin was quantitatively determined in culture filtrates and in mycelial extracts of the transformants. production of cu in vitro was associated with the ability to cause typical ded symptoms, ... | 2000 | 10656584 |
| isolation of the orthologue of the cerato-ulmin gene in ophiostoma quercus and characterization of the purified protein. | ophiostoma quercus is an ophiostomatoid fungus strictly related to the ophiostoma's (o. ulmi, o. novo-ulmi, and o. himal-ulmi) that cause dutch elm disease (ded). o. quercus has a number of morphological characteristics in common with the ded pathogens, and is a well-known and economically important sapstaining fungus occurring worldwide on hardwoods and commercially produced pines, and causes typical cankers on oak stems. in elm trees o. quercus can survive for months without causing any diseas ... | 2008 | 18783930 |
| ophiostoma tsotsi sp. nov., a wound-infesting fungus of hardwood trees in africa. | polymorphic sequence-characterised marker assays from a recent diversity study on the ascomycete fungus ophiostoma quercus reported that some isolates from africa were genetically distinct from o. quercus. in the present study, these african isolates were compared with authentic o. quercus isolates by evaluating morphological characters, growth in culture, mating compatibility and dna sequence data. the isolates from africa were morphologically similar to o. quercus, presenting pesotum and sporo ... | 2010 | 20054655 |
| development and characterization of polymorphic markers for the sap-stain fungus ophiostoma quercus. | eight polymorphic markers were developed from south african isolates of ophiostoma quercus. the genome was screened for repeat regions using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol and 20 de novo primer pairs flanking putative microsatellite regions were designed. eight loci were optimized and their polymorphisms evaluated by sequencing. the repeat and flanking regions were highly polymorphic containing both indels and base-pair subst ... | 2009 | 21564661 |
| temperature-dependent changes in the microbial storage flora of birch and spruce sawdust. | sawdust can be used to make pellets (biofuel) and particle boards and as a potential lignocellulose feedstock in bioethanol production. microbial activity can affect sawdust quality; hence, we monitored the microbial population in birch- and spruce sawdust after 3 months' storage at various temperatures. species composition was similar on both materials but was strongly influenced by temperature. bacteria were present on all materials at all conditions: on birch, 2.8 × 10(8) , 1.1 × 10(8) , and ... | 2014 | 24527731 |
| both mating types in the heterothallic fungus ophiostoma quercus contain mat1-1 and mat1-2 genes. | in heterothallic ascomycota, two opposite but distinct mating types control all sexual processes. using mating crosses, mating types were assigned to ten isolates of the heterothallic fungal species ophiostoma quercus. primers were subsequently designed to target the mat1-1-1, mat1-1-3 (of the mating type 1 idiomorph), and mat1-2-1 (of the mating type 2 idiomorph) genes in these isolates. results showed that all isolates contained the full gene sequence for the mat1-2-1 gene. in addition, fragme ... | 2012 | 22385624 |