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bovine arboviruses in culicoides biting midges and sentinel cattle in southern japan from 2003 to 2013.epizootic congenital abnormalities, encephalomyelitis and febrile illnesses in cattle caused by arthropod-borne viruses (arboviruses) are prevalent in japan. causative viruses including orthobunyaviruses, orbiviruses and rhabdovirus are thought to be transmitted by culicoides biting midges. recently, the incursions of several arboviruses, potentially culicoides-borne, were newly confirmed in japan. however, their spread pattern and exact vector species are currently uncertain. attempts to isolat ...201625597441
isolation and preliminary characterization of an orbivirus of the palyam serogroup from biting midge culicoides oxystoma in japan.an orbivirus of the palyam serogroup was isolated from culicoides oxystoma collected in a cowshed in kagoshima, southern kyushu island, japan. this is the first isolation of an orbivirus of the palyam serogroup in japan. the virus was a spherical non-enveloped rna virus, approximately 60 nm in diameter. the virus was resistant to ethyl ether, sodium deoxycholate and freezing-thawing, but readily inactivated by trypsin. the virus was not stabilized by 1 m mgcl2, was labile at ph 3.0 and was not p ...19892646823
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