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arcanobacterium pluranimalium sp. nov., isolated from porpoise and deer.two strains of a previously undescribed arcanobacterium-like bacterium were isolated from a dead harbour porpoise and a dead sallow deer. biochemical testing and page analysis of whole-cell proteins indicated that the strains were phenotypically closely related to each other and distinct from previously described actinomyces and arcanobacterium species. comparative 16s rrna gene sequencing studies showed the bacterium to be a hitherto unknown subline within the genus arcanobacterium. based on ph ...200111211273
arcanobacterium hippocoleae sp. nov., from the vagina of a horse.a polyphasic taxonomic study was performed on a previously unidentified gram-positive, facultatively anaerobic, diphtheroid-shaped organism isolated from a vaginal discharge of a horse. comparative 16s rrna gene sequencing demonstrated that the strain was a member of the genus arcanobacterium, but sequence divergence values of >4% with described species of this genus (viz: arcanobacterium haemolyticum, arcanobacterium bernardiae, arcanobacterium phocae, arcanobacterium pluranimalium and arcanoba ...200211931175
identification of arcanobacterium pluranimalium isolated from a dog by phenotypic properties and by pcr mediated characterization of various molecular targets. 201019913362
distribution of arcanobacterium pluranimalium in animals examined in veterinary laboratories in the united kingdom.arcanobacterium pluranimalium was first reported in 2001 for 2 isolates, from a harbor porpoise (phocoena phocoena) and a fallow deer (dama dama). the same organism was subsequently reported from ovine material. a review of material submitted to veterinary laboratories in the united kingdom from a range of domestic and wild animals during the following years revealed that a. pluranimalium had been recovered from ovine specimens on 33 occasions. twenty ovine isolates came from abortion material, ...201121908355
phenotypical and genotypical properties of an arcanobacterium pluranimalium strain isolated from a juvenile giraffe (giraffa camelopardalis reticulata).the present study was designed to characterize phenotypically and genotypically an arcanobacterium pluranimalium strain (a. pluranimalium 4868) following necropsy from a juvenile giraffe. the species identity could be confirmed by phenotypical investigations and by maldi-tof ms analysis, by sequencing the 16s rdna, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to a. pluranimalium reference strain dsm 13483(t) of 99 ...201426464930
isolation of actinomyces hyovaginalis from sheep and comparison with isolates obtained from pigs.actinomyces hyovaginalis, an organism initially described from pigs, was recovered from nine sheep and a moufflon. further strains of a. hyovaginalis were recovered from five samples from pigs over the same period. 16s rrna sequencing and extensive phenotyping demonstrated high similarity between the ovine and porcine isolates; however differences with respect to erythritol, adonitol and l-arabitol fermentation were detected. ovine isolates were made from various sample sites including abscesses ...201222266161
phenotypic and genotypic approach to characterize arcanobacterium pluranimalium isolated from bovine milk samples.in the present study, three arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) analysis and genotypically by sequencing the molecular targets 16s rdna, 16s-23s rdna intergenic spacer region (isr), the β subunit of bacterial rna polymerase encoding gene ...201626883140
development of a loop-mediated isothermal amplification (lamp) assay for rapid and sensitive identification of arcanobacterium pluranimalium.in the present study 28 arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (lamp) assay based on gene pla encoding pluranimaliumlysin. no comparable reaction could be observed with control strains representing five species of genus arcanobacterium and five species of genus trueperella. the presented pla lamp assay might allow a reliable and low cost identification of this bacterial path ...201526093093
identification of arcanobacterium pluranimalium by matrix-assisted laser desorption ionization-time of flight mass spectrometry and, as novel target, by sequencing pluranimaliumlysin encoding gene pla.in the present study 13 arcanobacterium pluranimalium strains isolated from various animal origin could successfully be identified phenotypically by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) and genotypically by sequencing 16s rdna and the pluranimaliumlysin encoding gene pla. the detection of mass spectra by maldi-tof ms and the novel genotypic approach using gene pla might help to identify a. pluranimalium in future and might elucidate the role ...201424345409
arcanobacterium pluranimalium leading to a bovine mastitis: species identification by a newly developed pla gene based pcr.we are describing a clinical case of bovine mastitis due to arcanobacterium pluranimalium in a holstein-friesian heifer, delivering bloody milk on the left hindquarter. moreover, we report on the development and evaluation of pcr primers based on the pluranimaliumlysin (pla) gene for the identification of this species. with the primer pair plaf/plar the a. pluranimalium type strain as well as the mastitis isolate 704 revealed a correctly sized amplification product (458 bp), whereas no amplifica ...201323732384
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