characterization of rhizobia that nodulate legume species of the genus lespedeza and description of bradyrhizobium yuanmingense sp. nov.legume species belonging to the genus lespedeza are annual or perennial herb or shrub plants that grow in the northern hemisphere. they are known for the formation of root nodules, but little information is available about their microsymbionts. in this study, 58 root-nodule isolates from lespedeza spp., obtained from china and the usa, were characterized using numerical taxonomic analysis of phenotypic features, sds-page analysis of whole-cell proteins, dna-dna hybridization, 16s rrna gene seque ...200212508891
bradyrhizobium betae sp. nov., isolated from roots of beta vulgaris affected by tumour-like deformations.some varieties of sugar beet, beta vulgaris, cultivated in northern spain have large deformations that resemble the tumours produced by agrobacterium species. in an attempt to isolate the agent responsible for these deformations, several endophytic slow-growing bacterial strains were isolated, the macroscopic morphology of which resembled that of bradyrhizobium species. these strains were not able to produce tumours in nicotiana tabacum plants and, based on phylogenetic analysis of their 16s rrn ...200415280302
molecular diversity of native bradyrhizobia isolated from lima bean (phaseolus lunatus l.) in peru.the diversity of a collection of 21 bradyrhizobial isolates from lima bean (phaseolus lunatus l.) was assayed by molecular methods. moderately high to high genetic diversity was revealed by multilocus enzyme electrophoresis (mlee) analysis of seven enzyme loci and genomic fingerprints with eric and box primers. two groups with differences in growth rate were found among the isolates and their differentiation as two divergent bradyrhizobial lineages was supported by pcr-rflp of the rpob gene and ...200616564961
genetic diversity of rhizobia associated with desmodium species grown in china.desmodia are leguminous plants used as important forage and herbal medicine in china. little information is available about the nodule bacteria of desmodium species. to understand the genetic diversity of rhizobia associated with desmodium species grown in china, isolates from temperate and subtropical regions were obtained and analysed.200717309506
bradyrhizobium elkanii, bradyrhizobium yuanmingense and bradyrhizobium japonicum are the main rhizobia associated with vigna unguiculata and vigna radiata in the subtropical region of china.cowpea (vigna unguiculata) and mung bean (vigna radiata) are important legume crops yet their rhizobia have not been well characterized. in the present study, 62 rhizobial strains isolated from the root nodules of these plants grown in the subtropical region of china were analyzed via a polyphasic approach. the results showed that 90% of the analyzed strains belonged to or were related to bradyrhizobium japonicum, bradyrhizobium liaoningense, bradyrhizobium yuanmingense and bradyrhizobium elkani ...200818564340
evidence from internally transcribed spacer sequence analysis of soybean strains that extant bradyrhizobium spp. are likely the products of reticulate evolutionary events.the internally transcribed spacer (its) sequences of several members within each of 17 soybean bradyrhizobial serogroups were determined to establish whether the regions within all members of each serogroup were identical. the rationale was to provide a sequence-based alternative to serology. the objective also was to link the extensive older literature on soybean symbiosis based on serology with its sequence data for more recent isolates from both soybean and other legumes nodulated by rhizobia ...200919011081
genetic diversity and distribution of bradyrhizobium and azorhizobium strains associated with the herb legume zornia glochidiata sampled from across senegal.herb legumes have great potential for rehabilitation of semi-arid degraded soils in sahelian ecosystems as they establish mutualistic symbiosis with n(2)-fixing rhizobia. a phylogenetic analysis was performed for 78 root nodule bacteria associated with the common sahelian herb legume zornia glochidiata reichb ex dc in senegal. based on its (rdna16s-23s) and reca sequences, these strains were shown to belong to the two genera bradyrhizobium and azorhizobium. strains of this latter, although frequ ...200919493641
vigna mungo, v. radiata and v. unguiculata plants sampled in different agronomical-ecological-climatic regions of india are nodulated by bradyrhizobium yuanmingense.vigna mungo, vigna radiata and vigna unguiculata are important legume crops cultivated in india, but little is known about the genetic resources in native rhizobia that nodulate these species. to identify these bacteria, a core collection of 76 slow-growing isolates was built from root nodules of v. mungo, v. radiata and v. unguiculata plants grown at different sites within three agro-ecological-climatic regions of india. the genetic diversity of the bacterial collection was assessed by restrict ...200919541444
genetic diversity of native soybean bradyrhizobia from different topographical regions along the southern slopes of the himalayan mountains in nepal.soybean-nodulating bradyrhizobia are genetically diverse and are classified into different species. in this study, the genetic diversity of native soybean bradyrhizobia isolated from different topographical regions along the southern slopes of the himalayan mountains in nepal was explored. soil samples were collected from three different topographical regions with contrasting climates. a local soybean cultivar, cobb, was used as a trap plant to isolate bradyrhizobia. a total of 24 isolates selec ...201020851547
genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (glycine soja sieb. & zucc.) in different ecoregions of china.a total of 99 bacterial isolates that originated from root nodules of glycine soja were characterized with restriction analyses of amplified 16s ribosomal dna and 16s-23s rdna intergenic spacers (its), and sequence analyses of 16s rrna, rpob, atpd, reca and nodc genes. when tested for nodulation of g. soja, 72 of the isolates were effective symbionts, and these belonged to five species: bradyrhizobium japonicum, bradyrhizobium elkanii, bradyrhizobium yuanmingense, bradyrhizobium liaoningense and ...201121303397
diversity and biogeography of rhizobia isolated from root nodules of glycine max grown in hebei province, china.a total of 215 rhizobial strains were isolated and analyzed with 16s rrna gene, 16s-23s intergenic spacer, housekeeping genes atpd, reca, and glnii, and symbiotic genes nifh and nodc to understand the genetic diversity of soybean rhizobia in hebei province, china. all the strains except one were symbiotic bacteria classified into nine genospecies in the genera of bradyrhizobium and sinorhizobium. surveys on the distribution of these rhizobia in different regions showed that bradyrhizobium japoni ...201121340735
phylogeny of bradyrhizobia from chinese cowpea miscellany inferred from 16s rrna, atpd, glnii, and 16s-23s intergenic spacer sequences.the cowpea (vigna unguiculata l.), peanut (arachis hypogaea l.), and mung bean (vigna radiata l.) belong to a group of plants known as the "cowpea miscellany" plants, which are widely cultivated throughout the tropic and subtropical zones of africa and asia. however, the phylogeny of the rhizobial strains that nodulate these plants is poorly understood. previous studies have isolated a diversity of rhizobial strains from cowpea miscellany hosts and have suggested that, phylogenetically, they are ...201121491983
characterization of cyclic ╬▓-glucans of bradyrhizobium by maldi-tof mass spectrometry.periplasmic, cyclic ╬▓-glucans isolated from bradyrhizobium elkanii, bradyrhizobium liaoningense, and bradyrhizobium yuanmingense strains have been investigated by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms), 1d and 2d nuclear magnetic resonance (nmr), as well as standard chemical methods. these compounds are built of 10-13 d-glucose residues. the main fractions contain molecules assembled of 12 hexose units (m(w)=1945.363da). glucose mono ...201121665197
genotypic characterization of phage-typed indigenous soybean bradyrhizobia and their host range symbiotic effectiveness.analysis of genetic diversity among indigenous rhizobia and its symbiotic effectiveness with soybean cultivar is important for development of knowledge about rhizobial ecology. in india, little is known about the genetic resources and diversity of rhizobia nodulating soybean. indigenous bradyrhizobia isolated from root nodules of soybean plants, collected from traditional cultivating regions of two states (madhya pradesh and uttar pradesh) of india, were screened for bacteriophage sensitivity to ...201121984348
[genetic diversity and phylogeny of bradyrhizobia associated with desmodium spp].genetic diversity and phylogeny of bradyrhizobial strains associated with desmodium spp. in subtropic and temperate regions of china were analyzed.201122233051
rhizobia indigenous to the okavango region in sub-saharan africa: diversity, adaptations, and host specificity.the rhizobial community indigenous to the okavango region has not yet been characterized. the isolation of indigenous rhizobia can provide a basis for the formulation of a rhizobial inoculant. moreover, their identification and characterization contribute to the general understanding of species distribution and ecology. isolates were obtained from nodules of local varieties of the pulses cowpea, bambara groundnut, peanut, hyacinth bean, and common bean. ninety-one of them were identified by box ...201425239908
genetic divergence of bradyrhizobium strains nodulating soybeans as revealed by multilocus sequence analysis of genes inside and outside the symbiosis island.the genus bradyrhizobium has been considered to be a taxonomically difficult group. in this study, phylogenetics and evolutionary genetics analyses were used to investigate divergence levels among bradyrhizobium strains nodulating soybeans in china. eleven genospecies were identified by sequence analysis of three phylogenetic and taxonomic markers (smc00019, thra, and trua). this was also supported by analyses of eight genes outside the symbiosis island ("off-island" genes; smc00019, thra, trua, ...201424632260
bradyrhizobium daqingense sp. nov., isolated from soybean nodules.thirteen slow-growing rhizobial strains isolated from root nodules of soybean (glycine max l.) grown in daqing city in china were classified in the genus bradyrhizobium based on 16s rrna gene sequence analysis. multilocus sequence analysis of igs, atpd, glnii and reca genes revealed that the isolates represented a novel clade in this genus. dna-dna relatedness lower than 42.5 % between the representative strain ccbau 15774(t) and the type strains of the closely related species bradyrhizobium lia ...201322544787
genetic diversity and evolution of bradyrhizobium populations nodulating erythrophleum fordii, an evergreen tree indigenous to the southern subtropical region of china.the nodulation of erythrophleum fordii has been recorded recently, but its microsymbionts have never been studied. to investigate the diversity and biogeography of rhizobia associated with this leguminous evergreen tree, root nodules were collected from the southern subtropical region of china. a total of 166 bacterial isolates were obtained from the nodules and characterized. in a pcr-based restriction fragment length polymorphism (rflp) analysis of ribosomal intergenic sequences, the isolates ...201425085491
wild peanut arachis duranensis are nodulated by diverse and novel bradyrhizobium species in acid soils.aiming at learning the microsymbionts of arachis duranensis, a diploid ancestor of cultivated peanut, genetic and symbiotic characterization of 32 isolates from root nodules of this plant grown in its new habitat guangzhou was performed. based upon the phylogeny of 16s rrna, atpd and reca genes, diverse bacteria belonging to bradyrhizobium yuanmingense, bradyrhizobium elkanii, bradyrhizobium iriomotense and four new lineages of bradyrhizobium (19 isolates), rhizobium/agrobacterium (9 isolates), ...201424985193
bradyrhizobium arachidis sp. nov., isolated from effective nodules of arachis hypogaea grown in china.twenty-three bacterial strains isolated from root nodules of arachis hypogaea and lablab purpureus grown in five provinces of china were classified as a novel group within the genus bradyrhizobium by analyses of pcr-based rflp of the 16s rrna gene and 16s-23s igs. to determine their taxonomic position, four representative strains were further characterized. the comparative sequence analyses of 16s rrna and six housekeeping genes clustered the four strains into a distinctive group closely related ...201323295123
symbiotic n 2 -fixation estimated by the (15) n tracer technique and growth of pueraria phaseoloides (roxb.) benth. inoculated with bradyrhizobium strain in field conditions.this field experiment was established in eastern cameroon to examine the effect of selected rhizobial inoculation on n2-fixation and growth of pueraria phaseoloides. treatments consisted of noninoculated and bradyrhizobium yuanmingense s3-4-inoculated pueraria with three replications each. ipomoea batatas as a non-n2-fixing reference was interspersed in each pueraria plot. all the twelve plots received 2 gn/m(2) of (15)n ammonium sulfate 10% atom excess. at harvest, dry matter yields and the nit ...201626904363
bradyrhizobium vignae sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of vigna and arachis.twenty one strains of symbiotic bacteria from root nodules of local races of cowpea (vigna unguiculata), bambara groundnut (vigna subterranea) and peanuts (arachis hypogaea) grown on subsistence farmers' fields in the kavango region of namibia, were previously characterized as a novel group within the genus bradyrhizobium. to verify their taxonomic position, the strains were further analysed using a polyphasic approach. 16s rrna gene sequences were most similar to bradyrhizobium manausense br 33 ...201626463703
bradyrhizobium guangdongense sp. nov. and bradyrhizobium guangxiense sp. nov., isolated from effective nodules of slow-growing rhizobia isolated from effective nodules of arachis hypogaea were assigned to the genus bradyrhizobium based on sharing 96.3-99.9 % 16s rrna gene sequence similarity with the type strains of recognized bradyrhizobium species. multilocus sequence analysis of glnii, reca, gyrb and dnak genes indicated that the seven strains belonged to two novel species represented by ccbau 51649t and ccbau 53363t. strain ccbau 51649t shared 94, 93.4, 92.3 and 94.9 % and ccbau 53363t shared 91.4 ...201526409482
bradyrhizobium subterraneum sp. nov., a symbiotic nitrogen-fixing bacterium from root nodules of strains of symbiotic bacteria from root nodules of local races of bambara groundnut (vigna subterranea) and peanuts (arachis hypogaea) grown on subsistence farmers' fields in the kavango region, namibia, were previously characterized and identified as a novel group within the genus bradyrhizobium. to corroborate their taxonomic status, these strains were further characterized using a polyphasic approach. all strains possessed identical 16s rrna gene sequences with bradyrhizobium yuanmingen ...201526198108
vigna unguiculata is nodulated in spain by endosymbionts of genisteae legumes and by a new symbiovar (vignae) of the genus bradyrhizobium.vigna unguiculata was introduced into europe from its distribution centre in africa, and it is currently being cultivated in mediterranean regions with adequate edapho-climatic conditions where the slow growing rhizobia nodulating this legume have not yet been studied. previous studies based on rrs gene and its region analyses have shown that bradyrhizobium yuanmingense and b. elkanii nodulated v. unguiculata in africa, but these two species were not found in this study. using the same phylogene ...201424867807
surviving and thriving in terms of symbiotic performance of antibiotic and phage-resistant mutants of bradyrhizobium of soybean [glycine max (l.) merrill].rhizobial inoculation plays an important role in yielding enhancement of soybean, but it is frequently disturbed by competition with bacterial population present in the soil. identification of potential indigenous rhizobia as competitive inoculants for efficient nodulation and n(2)-fixation of soybean was assessed under laboratory and field conditions. two indigenous bradyrhizobial isolates (mpsr033 and mpsr220) and its derived different antibiotic (streptomycin and gentamicin) and phage (rt5 an ...201222735983
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