Publications

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a laboratory technique for culturing filipievimermis leipsandra, a nematode parasite of diabrotica balteata larvae (insecta: coleoptera). 198119300750
identification of female-produced sex pheromone from banded cucumber beetle,diabrotica balteata leconte (coleoptera: chrysomelidae).a sex pheromone produced by female banded cucumber beetle adults,diabrotica balteata leconte, was isolated from volatiles trapped on porapak q and identified as 6,12-dimethylpentadecan-2-one. the structure was elucidated by spectroscopic analyses and confirmed by synthesis. the synthesized racemic compound was equal to the purified natural pheromone in eliciting responses by banded cucumber beetle males to field traps. a doseresponse characteristic was demonstrated for the racemic material formu ...198724302330
effect of temperature on development and survival of the mermithid filipjevimermis leipsandra.the development and survival of egg, juvenile (parasitic), preovipositing, and ovipositing stages of the parasitic mermithid filipjevimermis leipsandra were determined at eight temperatures. infection of the host, diabrotica balteata, peaked at 30 c and fell off sharply at warmer or cooler temperatures. no development, oviposition, or infection occurred at < 20 c. fecundity was highest at 25 c, and 50% of the eggs were laid in the first 4-7 days of oviposition.198919287629
overwintering and cold tolerance of the mermithid filipjevimermis leipsandra.overwintering survival of filipjevimermis leipsandra postparasitic adults ranged from 12 to 15% over 2 years. nematodes recovered from biweekly samples oviposited within a week when exposed to warm temperatures. more nematodes were recovered from the lower portions of soil cores than from the upper. in laboratory tests of cold tolerance, the ld at -5 c was 5.5 hours, whereas exposure to -2 c for as long as 96 hours resulted in no mortality.199119283133
identification, quantification, spatiotemporal distribution and genetic variation of major latex secondary metabolites in the common dandelion (taraxacum officinale agg.).the secondary metabolites in the roots, leaves and flowers of the common dandelion (taraxacum officinale agg.) have been studied in detail. however, little is known about the specific constituents of the plant's highly specialized laticifer cells. using a combination of liquid and gas chromatography, mass spectrometry and nuclear magnetic resonance spectrometry, we identified and quantified the major secondary metabolites in the latex of different organs across different growth stages in three g ...201525682510
specificity of induced defenses, growth, and reproduction in lima bean (phaseolus lunatus) in response to multispecies herbivory.201526290553
synthesis of (6r,12r)-6,12-dimethylpentadecan-2-one, the female-produced sex pheromone from banded cucumber beetle diabrotica balteata, based on a chiron approach.herein we describe a synthesis of (6r,12r)-6,12-dimethylpentadecan-2-one (5), the female produced sex pheromone of banded cucumber beetle diabrotica balteata le conte, from (r)-4-methyl-5-valerolactone, a methyl-branched chiron.201526882689
a comparison, by sweep sampling, of the insect fauna from corn and sweet potato monocultures and dicultures in costa rica.the insect fauna of 80 day-old plots of corn and sweet potato monocultures and dicultures in costa rica were compared using sweep sampling. six hundred sweeps were taken in each of the three habitats. there were 15% more total species in the diculture than either monoculture but approximately the same total number of individuals. there were 75% more species and approximately 100% more individuals of parasitic hymenoptera in the diculture than the monocultures. the ratio of numbers of phytophagou ...197928309660
selinene volatiles are essential precursors for maize defense promoting fungal pathogen resistance.to ensure food security, maize (zea mays) is a model crop for understanding useful traits underlying stress resistance. in contrast to foliar biochemicals, root defenses limiting the spread of disease remain poorly described. to better understand belowground defenses in the field, we performed root metabolomic profiling and uncovered unexpectedly high levels of the sesquiterpene volatile β-selinene and the corresponding nonvolatile antibiotic derivative β-costic acid. the application of metaboli ...201728931629
enzyme induction as a possible mechanism for latex-mediated insect resistance in romaine lettuce.plant latex is a known storehouse of various secondary metabolites with demonstrated negative impact on insect fitness. a romaine lettuce cultivar, "valmaine", possesses a high level of latex-mediated resistance against the banded cucumber beetle, diabrotica balteata leconte (coleoptera: chrysomelidae), compared to a closely related cultivar "tall guzmaine". latex from damaged valmaine plants was much more deterrent to adult d. balteata feeding than latex from undamaged plants when applied to th ...200919184224
resistance to adult banded cucumber beetle (coleoptera: chrysomelidae) in romaine lettuce.four cultivars of lettuce, lactuca sativa l., were evaluated for their resistance to the adult banded cucumber beetle, diabrotica balteata leconte, under laboratory conditions. when paired with each of the other three cultivars, leaf consumption in all possible combinations of short-term (48 h) two-choice tests among the cultivars was significantly reduced only for 'valmaine'. however, in a 48-h no-choice situation, beetles fed valmaine or 'short guzmaine' had similarly low leaf consumption, fol ...200212216830
polycyclic spiro lignans and biphenyl tetrahydrofuranone lignans from gymnotheca involucrata.four rare polycyclic spiro lignans (1-4) and four new biphenyl tetrahydrofuranone lignans (5-8) were isolated from the whole plant of gymnotheca involucrata. the structures of the new compounds were elucidated on the basis of detailed spectroscopic analysis and the absolute configuration of 1 was confirmed by single crystal x-ray diffraction. bioassay results showed that compounds 2 and 6 exhibited weak antifungal activity against uromyces viciae-fabae at 100 ppm in leaf-disc assays, while compo ...201626872322
heterorhabditis sp. (nematoda: heterorhabditidae): a nematode parasite isolated from the banded cucumber beetle diabrotica balteata.a nematode identified as heterorhabditis sp. was discovered in june 1982 in larval cadavers of the banded cucumber beetle, diabrotica balteata, in soil on wooded land. effective beetle control (over 95%) was obtained when larvae were exposed to potted soil containing infective stage nematode juveniles or infected larval cadavers. the nematode was propagated in vivo on larvae of d. balteata, diaphania nitidalis (the pickleworm), and galleria mellonella (the greater wax moth). this heterorhabditis ...198519294074
a specialist root herbivore exploits defensive metabolites to locate nutritious tissues.the most valuable organs of plants are often particularly rich in essential elements, but also very well defended. this creates a dilemma for herbivores that need to maximise energy intake while minimising intoxication. we investigated how the specialist root herbivore diabrotica virgifera solves this conundrum when feeding on wild and cultivated maize plants. we found that crown roots of maize seedlings were vital for plant development and, in accordance, were rich in nutritious primary metabol ...201222070646
resistance of sweetpotato genotypes to adult diabrotica beetles.production of sweetpotatoes, ipomoea batatas (l.) lam. (convolvulaceae), is limited by several insect pests, including diabrotica spp. (coleoptera: chrysomelidae), and new integrated pest management (ipm) techniques for this crop are needed. host plant resistance is one attractive approach that fits well into ipm programs. a host plant resistance research program typically depends on reliable bioassay procedures to streamline evaluation of germplasm. thus, a bioassay technique was developed for ...200717461084
partitioning yield loss on yellow squash into nematode and insect components.the effect of a contplex of several insect and nematode pests on yield of yellow squash (cucurbita pepo l.) was examined in two field tests in southern florida. applications of permethrin for insect control and oxamyl primarily for nematode control plus some insect control were made alone and in combination to achieve differential reduction of various insect and nematode components contributing to yield loss. the effect of these components on yield was further analyzed by multiple regression. yi ...198219295683
monitoring cucumber beetles in sweetpotato and cucurbits with kairomone-baited traps.seven kairomone formulations (trécé, inc., salinas, ca) were evaluated for their effectiveness as attractants for luring three species of cucumber beetles into pherocon crw traps (trécé, inc.) in cucurbit and sweetpotato fields. the spotted cucumber beetle, diabrotica undecimpunctata howardi barber; the banded cucumber beetle, diabrotica balteata leconte; and the striped cucumber beetle, acalymma vittatum (f.), were captured in this study. tre8276 (tic mixture: 500 mg of 1,2,4-trimethoxybenzene, ...200515765678
accumulation of terpenoid phytoalexins in maize roots is associated with drought tolerance.maize (zea mays) production, which is of global agro-economic importance, is largely limited by herbivore pests, pathogens and environmental conditions, such as drought. zealexins and kauralexins belong to two recently identified families of acidic terpenoid phytoalexins in maize that mediate defence against both pathogen and insect attacks in aboveground tissues. however, little is known about their function in belowground organs and their potential to counter abiotic stress. in this study, we ...201525392907
in vitro cultivation of the entomogenous nematode filipjevimermis leipsandra.the mermithid nematode, filipjevimermis leipsandra, was successfully cultivated to the preadult stage in schneider's drosophila medium supplemented with 20% fetal bovine serum. upon transfer to a solid substrate the preadults continued to develop into ovipositing adult females. four molts were observed. the first molt occurred in the egg. the second occurred after 6-8 days in culture during which the very thin cuticle was shed completely. the third molt occurred after 18-20 days in culture; the ...198219295686
induced jasmonate signaling leads to contrasting effects on root damage and herbivore performance.induced defenses play a key role in plant resistance against leaf feeders. however, very little is known about the signals that are involved in defending plants against root feeders and how they are influenced by abiotic factors. we investigated these aspects for the interaction between rice (oryza sativa) and two root-feeding insects: the generalist cucumber beetle (diabrotica balteata) and the more specialized rice water weevil (lissorhoptrus oryzophilus). rice plants responded to root attack ...201525627217
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