| molecular characterization of a new hypovirus infecting a phytopathogenic fungus, valsa ceratosperma. | a double-stranded (ds) rna, approximately 9.5kb in size; was identified in the mvc86 isolate of valsa ceratosperma. complete sequence of the dsrna revealed a 9543-bp segment (excluding the 3' poly-a tail) that is predicted to encode a single large protein (p330). p330 has 63%, 49%, and 55% amino acid sequence identities to the proteins encoded by hypoviruses cryphonectria hypovirus 3 (chv3), chv4, and sclerotinia sclerotiorum hypovirus 1 (sshv1), respectively. like polyproteins encoded by chv3, ... | 2012 | 22366520 |
| satellite and defective rnas of cryphonectria hypovirus 3-grand haven 2, a virus species in the family hypoviridae with a single open reading frame. | cryphonectria parasitica hypovirus 3-grand haven 2 (chv3-gh2) is the most recently characterized member of the hypoviridae family of viruses associated with hypovirulence of the chestnut blight fungus. isolates of chv3-gh2 contain either three or four double-stranded (ds) rnas that are visible on ethidium bromide-stained agarose or polyacrylamide gels. only the largest dsrna appears to be required for virus infectivity, and was characterized previously (c. d. smart et al., 1999, virology 265, 66 ... | 2000 | 11022006 |
| cryphonectria hypovirus 3, a virus species in the family hypoviridae with a single open reading frame. | isolate grand haven (gh) 2 is a naturally occurring isolate of the chestnut blight fungus, cryphonectria parasitica, that is greatly reduced in virulence due to the presence of a double-stranded rna virus. unlike many other virus-infected, hypovirulent isolates, gh2 is not substantially reduced in pigmentation, conidiation, or laccase expression compared to its virus-free counterpart. the dsrna genome of the gh2 virus was cloned, sequenced, and compared to hypovirulence-associated viruses of the ... | 1999 | 10603318 |
| in vitro translational analysis of genomic, defective, and satellite rnas of cryphonectria hypovirus 3-gh2. | cryphonectria hypovirus 3-gh2 (chv3-gh2) is a member of the fungal virus family hypoviridae that differs from previously characterized members in having a single large open reading frame with the potential to encode a protein of 326 kda from its 9.8-kb genome. the n-terminal portion of the orf contains sequence motifs that are somewhat similar to papain-like proteinases identified in other hypoviruses. translation of the orf is predicted to release autocatalytically a 32.5-kda protein. a defecti ... | 2001 | 11222102 |
| diversity of hypoviruses and other double-stranded rnas in cryphonectria parasitica in north america. | abstract double-stranded (ds) rnas in cryphonectria parasitica were randomly sampled from nine subpopulations in north america using an antibody-based detection system for dsrna. dsrna was detected in 166 (28%) of a total of 595 c. parasitica isolates sampled by immunoblotting. incidence of dsrna infection within subpopulations ranged from 0% in samples from new hampshire and ontario to 100% in county line, mi. most of the dsrnas sampled were approximately 9 to 13 kb in size. dsrnas from 72 isol ... | 1997 | 18945036 |
| cytological and electrophoretic karyotyping of the chestnut blight fungus cryphonectria parasitica. | the karyotypes of nine strains including three transformants of the chestnut blight fungus cryphonectria parasitica were analyzed by pulsed-field gel electrophoresis (pfge) and cytology using a fluorescence microscope. cytology of the mitotic metaphase showed n=9 for both standard strain ep155 and field strain gh2 infected by cryphonectria hypovirus 3. chromosomes were morphologically characterized by size, heterochromatic segment, and constriction. pfge resolved 5 or 6 chromosomal dna bands ran ... | 2009 | 19570503 |