| genotypic and phenotypic characterization of the acinetobacter calcoaceticus-acinetobacter baumannii complex with the proposal of acinetobacter pittii sp. nov. (formerly acinetobacter genomic species 3) and acinetobacter nosocomialis sp. nov. (formerly acinetobacter genomic species 13tu). | acinetobacter genomic species (gen. sp.) 3 and gen. sp. 13tu are increasingly recognized as clinically important taxa within the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex. to define the taxonomic position of these genomic species, we investigated 80 strains representing the known diversity of the acb complex. all strains were characterized by aflp analysis, amplified rdna restriction analysis and nutritional or physiological testing, while selected strains were studied by ... | 2011 | 21320596 |
| changes in antimicrobial susceptibility and major clones of acinetobacter calcoaceticus-baumannii complex isolates from a single hospital in korea over 7 years. | acinetobacter species have emerged as opportunistic nosocomial pathogens in intensive care units. epidemic spread and outbreaks of multidrug-resistant or carbapenem-resistant acinetobacter baumannii infections have been described worldwide. species distribution, antimicrobial resistance and genotypes were investigated for acinetobacter species isolates collected from a single institution in korea over 7 years. two hundred and eighty-seven acinetobacter species isolates were collected from patien ... | 2012 | 21852525 |
| acinetobacter infection - an emerging threat to human health. | the genus acinetobacter comprises a complex and heterogeneous group of bacteria, many of which are capable of causing a range of opportunistic, often catheter-related, infections in humans. however, acinetobacter baumannii, as well as its close relatives belonging to genomic species 3 ("acinetobacter pittii") and 13tu ("acinetobacter nosocomialis"), are important nosocomial pathogens, often associated with epidemic outbreaks of infection, that are only rarely found outside of a clinical setting. ... | 2011 | 22006724 |
| emergence of carbapenem-resistant non-baumannii species of acinetobacter harboring a blaoxa-51-like gene that is intrinsic to a. baumannii. | the bla(oxa-51)-like gene, originally intrinsic to acinetobacter baumannii, had been detected in two clones of acinetobacter nosocomialis and one clone of acinetobacter genomic species "close to 13tu." these bla(oxa-51)-like genes, all preceded by isaba1, were located on plasmids that might have originated with a. baumannii. the plasmid-borne isaba1--bla(oxa-51)-like confers a high level of carbapenem resistance and affects the accuracy of using bla(oxa-51)-like detection as a tool for different ... | 2011 | 22083478 |
| Characteristics of carbapenem-resistant Acinetobacter spp. other than Acinetobacter baumannii in South Korea. | Although many studies have been performed on carbapenem-resistant Acinetobacter baumannii, only a few studies have addressed carbapenem resistance in Acinetobacter spp. other than A. baumannii (non-baumannii Acinetobacter). Amongst 287 Acinetobacter spp. isolates from patients with bacteraemia in a South Korean hospital collected between 2003 and 2010, 160 (55.7%) were non-baumannii Acinetobacter spp. Antimicrobial susceptibility testing was performed and the effect of efflux pump inhibitors was ... | 2012 | 21996405 |
| Nosocomial bloodstream infections due to Acinetobacter baumannii, 'Acinetobacterpittii' and 'Acinetobacternosocomialis' in the United States. | OBJECTIVES: To compare the clinical features and antimicrobial susceptibilities of the clinically most important Acinetobacter species Acinetobacterbaumannii, 'Acinetobacterpittii' (formerly Acinetobacter genomic species 3) and 'Acinetobacternosocomialis' (formerly Acinetobacter genomic species 13TU). METHODS: 295 Acinetobacter isolates collected prospectively from patients with bloodstream infections (BSI) in 52 US hospitals were identified to species level. Clinical and microbiological feature ... | 2011 | 22209744 |
| signature motifs identify an acinetobacter cif virulence factor with epoxide hydrolase activity. | endocytic recycling of the cystic fibrosis transmembrane conductance regulator (cftr) is blocked by the cftr inhibitory factor (cif). originally discovered in pseudomonas aeruginosa, cif is a secreted epoxide hydrolase that is transcriptionally regulated by cifr, an epoxide-sensitive repressor. in this report, we investigate a homologous protein found in strains of the emerging nosocomial pathogens acinetobacter nosocomialis and acinetobacter baumannii ("acif"). like cif, acif is an epoxide hydr ... | 2014 | 24474692 |
| medically relevant acinetobacter species require a type ii secretion system and specific membrane-associated chaperones for the export of multiple substrates and full virulence. | acinetobacter baumannii, a. nosocomialis, and a. pittii have recently emerged as opportunistic human pathogens capable of causing severe human disease; however, the molecular mechanisms employed by acinetobacter to cause disease remain poorly understood. many pathogenic members of the genus acinetobacter contain genes predicted to encode proteins required for the biogenesis of a type ii secretion system (t2ss), which have been shown to mediate virulence in many gram-negative organisms. here we d ... | 2016 | 26764912 |
| molecular epidemiology of acinetobacter baumannii bloodstream isolates obtained in the united states from 1995 to 2004 using rep-pcr and multilocus sequence typing. | using a repetitive-sequence-based (rep)-pcr (diversilab), we have molecularly typed acinetobacter nosocomial bloodstream isolates (acinetobacter baumannii [n = 187], acinetobacter pittii [n = 23], and acinetobacter nosocomialis [n = 61]) obtained from patients hospitalized in u.s. hospitals over a 10-year period (1995-2004) during a nationwide surveillance study (surveillance and control of pathogens of epidemiological importance [scope]). patterns of a. baumannii rep-pcr were compared to those ... | 2012 | 22895032 |
| abar4-type resistance island including the blaoxa-23 gene in acinetobacter nosocomialis isolates. | this study reports for the first time the abar4-type resistance island with the bla(oxa-23) gene in two carbapenem-resistant a. nosocomialis isolates from south korea and thailand. | 2012 | 22687501 |
| expression of the resistance-nodulation-cell division pump adeijk in acinetobacter baumannii is regulated by aden, a tetr-type regulator. | resistance-nodulation-division efflux system adeijk contributes to intrinsic resistance to various drug classes in acinetobacter baumannii. by whole-genome sequencing, we have identified in clinical isolate bm4587 the aden gene, located 813 kbp upstream from adeijk, which encodes a tetr transcriptional regulator. in one-step mutant bm4666 overexpressing adeijk, the deletion of cytosine 582 (c(582)) in the 3' portion of this gene was responsible for a frameshift mutation resulting in the deletion ... | 2012 | 22371895 |
| draft genome sequences of multidrug-resistant acinetobacter sp. strains from colombian hospitals. | the draft genome sequences of the strains acinetobacter baumannii 107m, acinetobacter nosocomialis 28f, and acinetobacter pittii 42f, isolated from colombian hospitals, are reported here. these isolates are causative of nosocomial infections and are classified as multidrug resistant, as they showed resistance to four different antibiotic groups. | 2013 | 24285656 |
| draft genome sequence of the clinical isolate acinetobacter nosocomialis strain m2. | we report the 3.78-mbp high-quality draft assembly of the genome from a clinical isolate of acinetobacter nosocomialis called strain m2 (previously known as acinetobacter baumannii strain m2). | 2013 | 24201195 |
| first report of carbapenem-resistant acinetobacter nosocomialis isolates harboring isaba1-blaoxa-23 genes in latin america. | in recent years, different resistance genes have been found in acinetobacter spp., especially in the species a. baumannii. we describe two isolates of carbapenem-resistant a. nosocomialis harboring isaba1-blaoxa-23 and blaoxa-51 found in patients from the city of porto alegre, southern brazil. to the best of the authors' knowledge, this is the first report of carbapenem-resistant a. nosocomialis in latin america. | 2013 | 23740725 |
| comparison between bacteremia caused by carbapenem resistant acinetobacter baumannii and acinetobacter nosocomialis. | it is unknown whether there are differences between bacteremia caused by carbapenem resistant acinetobacter baumannii (crab) and carbapenem resistant acinetobacter nosocomialis (cran). this study aims to investigate the differences, especially in clinical outcomes, between patients with bacteremia caused by crab or cran. | 2013 | 23841753 |
| pathogenic acinetobacter: from the cell surface to infinity and beyond. | the genus acinetobacter encompasses multiple nosocomial opportunistic pathogens that are of increasing worldwide relevance because of their ability to survive exposure to various antimicrobial and sterilization agents. among these, acinetobacter baumannii, acinetobacter nosocomialis, and acinetobacter pittii are the most frequently isolated in hospitals around the world. despite the growing incidence of multidrug-resistant acinetobacter spp., little is known about the factors that contribute to ... | 2016 | 26712938 |
| initial assessment of the molecular epidemiology of blandm-1 in colombia. | we report complete genome sequences of four blandm-1-harboring gram-negative multidrug-resistant (mdr) isolates from colombia. the blandm-1 genes were located on 193-kb inc fia, 178-kb inc a/c2, and 47-kb (unknown inc type) plasmids. multilocus sequence typing (mlst) revealed that these isolates belong to sequence type 10 (st10) (escherichia coli), st392 (klebsiella pneumoniae), and st322 and st464 (acinetobacter baumannii and acinetobacter nosocomialis, respectively). our analysis identified th ... | 2016 | 27067339 |
| structure and gene cluster of the k93 capsular polysaccharide of acinetobacter baumannii b11911 containing 5-n-acetyl-7-n-[(r)-3-hydroxybutanoyl]pseudaminic acid. | capsular polysaccharide (cps) assigned to the k93 type was isolated from the bacterium acinetobacter baumannii b11911 and studied by sugar analysis along with one- and two-dimensional (1)h and (13)c nmr spectroscopy. the cps was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. genes in the kl93 capsule biosynthesis locus were annotated and found to be consistent with the cps structure established. the k93 cps has ... | 2017 | 28371606 |
| lettuce and fruits as a source of multidrug resistant acinetobacter spp. | the role of ready-to-eat products as a reservoir of pathogenic species of acinetobacter remains unclear. the objective of the present study was to evaluate the presence of acinetobacter species in lettuces and fruits marketed in portugal, and their susceptibility to antimicrobials. acinetobacter spp. were isolated from 77.9% of the samples and these microorganisms were also found as endophytes (i.e. present within the plant tissue) in 12 of 20 samples of lettuces analysed. among 253 isolates tha ... | 2017 | 28213015 |
| genetic characterisation of carbapenem-resistant gram-negative bacteria isolated from the university hospital mohamed boudiaf in ouargla, southern algeria. | the aim of this study was to investigate the molecular epidemiology and the genetic support of carbapenem-resistant enterobacteriaceae and acinetobacter spp. isolates collected in the university hospital of ouargla, southern algeria. | 2017 | 28007519 |
| prevalence and antimicrobial susceptibility of acinetobacter spp. isolated from meat. | the prevalence and antibiotic resistance of acinetobacter spp. from fifty samples of meat (chicken, turkey, beef and pork) were evaluated. acinetobacter spp. was recovered from all samples and the clonal relatedness of 223 isolates identified to belong to the genus acinetobacter was established by pfge. a high genetic diversity was observed and 166 isolates from different samples, 141 representing different pfge profiles, were further identified to the species level by rpob gene sequencing. thir ... | 2017 | 27992816 |
| development and evaluation of species-specific pcr for detection of nine acinetobacter species. | molecular methods have the potential to improve the speed and accuracy of acinetobacter species identification in clinical settings. the goal of this study is to develop species-specific pcr assays based on differences in the rna polymerase beta-subunit gene (rpob) to detect nine commonly isolated acinetobacter species including acinetobacter baumannii, acinetobacter calcoaceticus, acinetobacter pittii, acinetobacter nosocomialis, acinetobacter lwoffii, acinetobacter ursingii, acinetobacter bere ... | 2016 | 27312551 |
| blood stream infections caused by acinetobacter baumannii group in japan - epidemiological and clinical investigation. | acinetobacter calcoaceticus-acinetobacter baumannii complex, especially a. baumannii, acinetobacter pittii and acinetobacter nosocomialis, constitutes an important group of nosocomial pathogens; however, epidemiological or clinical characteristics and prognosis is limited in japan. from 2009 to 2013, 47 blood stream infection cases resulting from a. baumannii group were reviewed at the national defense medical college, an 800-bed tertiary hospital. to determine the genospecies, further comparati ... | 2016 | 26993173 |
| katg and kate confer acinetobacter resistance to hydrogen peroxide but sensitize bacteria to killing by phagocytic respiratory burst. | catalase catalyzes the degradation of h2o2. acinetobacter species have four predicted catalase genes, kata, kate, katg, and katx. the aims of the present study seek to determine which catalase(s) plays a predominant role in determining the resistance to h2o2, and to assess the role of catalase in acinetobacter virulence. | 2016 | 26860891 |
| characterization of carbapenem-resistant acinetobacter calcoaceticus-baumannii complex isolates from nosocomial bloodstream infections in southern iran. | acinetobacter baumannii is an important opportunistic bacterial pathogen responsible for serious infections in hospitalized patients. from a total of 78 consecutive non-repetitive acinetobacter spp. isolates from patients with blood infections, 61 were carbapenem resistant, which were positive for blaoxa-51-like (96.7%), blaoxa-23-like (77 %), blaoxa-58-like (8.1%) and blaoxa-40-like genes (32.8%) by multiplex pcr. the isolates were identified as a. baumannii (n = 59) and acinetobacter nosocomia ... | 2016 | 26747061 |
| species identification within acinetobacter calcoaceticus-baumannii complex using maldi-tof ms. | acinetobacter baumannii, one of the more clinically relevant species in the acinetobacter genus is well known to be multi-drug resistant and associated with bacteremia, urinary tract infection, pneumonia, wound infection and meningitis. however, it cannot be differentiated from closely related species such as acinetobacter calcoaceticus, acinetobacter pittii and acinetobacter nosocomialis by most phenotypic tests and can only be differentiated by specific, time consuming genotypic tests with ver ... | 2015 | 26381662 |
| in vitro activity of aminoglycosides against clinical isolates of acinetobacter baumannii complex and other nonfermentative gram-negative bacilli causing healthcare-associated bloodstream infections in taiwan. | aminoglycosides possess in vitro activity against aerobic and facultative gram-negative bacilli. however, nationwide surveillance on susceptibility data of acinetobacter baumannii complex and pseudomonas aeruginosa to aminoglycosides was limited, and aminoglycoside resistance has emerged in the past decade. we study the in vitro susceptibility of a. baumannii complex and other nonfermentative gram-negative bacilli (nfgnb) to aminoglycosides. | 2016 | 26364729 |
| loss of lps is involved in the virulence and resistance to colistin of colistin-resistant acinetobacter nosocomialis mutants selected in vitro. | acinetobacter nosocomialis has increasingly been reported as an opportunistic pathogen causing nosocomial infections. although it is more susceptible to all antimicrobial agents than acinetobacter baumannii, mdr clinical isolates have also been described. in addition, several studies have shown a high percentage of resistance to colistin. therefore, in the present study we investigated the mechanism of resistance to colistin in this microorganism. | 2015 | 26311838 |
| using vitek maldi-tof mass spectrometry to identify species belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex: a relevant alternative to molecular biology? | acinetobacter baumannii belongs to the acinetobacter calcoaceticus-baumannii complex (acb) containing 2 other pathogenic species: acinetobacter pittii and acinetobacter nosocomialis. identification of these bacteria remains problematic despite the use of matrix-assisted laser ionization time-of-flight mass spectrometry (maldi-tof ms). here, we enriched the saramis™ database of the vitek ms® plus mass spectrometer to improve the identification of species of the acb complex. for each species, we i ... | 2015 | 26198329 |
| abar-type genomic islands in non-baumannii acinetobacter species isolates from south korea. | to investigate the presence and structure of abar-type genomic islands (gis) in non-acinetobacter baumannii isolates, a total of 155 non-baumannii acinetobacter isolates from a south korean hospital were analyzed. gis were found in three acinetobacter nosocomialis and two acinetobacter seifertii isolates. their structures were similar to those in a. baumannii isolates from asian countries, including south korea. the existence of abar-type gis in non-baumannii acinetobacter isolates is believed t ... | 2015 | 26100696 |
| risk factors and outcome for colistin-resistant acinetobacter nosocomialis bacteraemia in patients without previous colistin exposure. | the clinical characteristics of patients with colistin-resistant acinetobacter baumannii bacteraemia have been documented, but those of patients with bacteraemia caused by other acinetobacter species remain unknown. previous exposure to colistin has been shown to be associated with the emergence of colistin resistance, but may be not the only predisposing factor. in the current study, we highlight the risk and outcome of patients without previous exposure to colistin who acquired colistin-resist ... | 2015 | 25980356 |
| a distinct alleles and genetic recombination of pmrcab operon in species of acinetobacter baumannii complex isolates. | to investigate pmrcab sequence divergence in 5 species of acinetobacter baumannii complex, a total of 80 isolates from a korean hospital were explored. we evaluated nucleotide and amino acid polymorphisms of pmrcab operon, and phylogenetic trees were constructed for each gene of prmcab operon. colistin and polymyxin b susceptibility was determined for all isolates, and multilocus sequence typing was also performed for a. baumannii isolates. our results showed that each species of a. baumannii co ... | 2015 | 25934155 |
| comparison between bacteremia caused by acinetobacter pittii and acinetobacter nosocomialis. | patients with acinetobacter pittii and acinetobacter nosocomialis bacteremia have lower mortality rates than those with acinetobacter baumannii bacteremia. however, it is unknown whether these organisms differ in outcomes of bacteremic patients. we conducted this study to answer this question. | 2017 | 25735795 |
| dissemination of blaoxa-23 in acinetobacter spp. in china: main roles of conjugative plasmid pazj221 and transposon tn2009. | production of the oxa-23 carbapenemase is the most common reason for the increasing carbapenem resistance in acinetobacter spp. this study was conducted to reveal the genetic basis of blaoxa-23 dissemination in acinetobacter spp. in china. a total of 63 carbapenem-resistant oxa-23-producing acinetobacter sp. isolates, representing different backgrounds, were selected from 28 hospitals in 18 provinces for this study. generally, two patterns of plasmids carrying blaoxa-23 were detected according t ... | 2015 | 25605357 |
| [frequency and antimicrobial resistance of acinetobacter species in a university hospital of buenos aires city]. | two-hundred acinetobacter isolates belonging to 200 patients admitted to hospital de clínicas josé de san martín during the period march 2013-june 2014 were analyzed. the identification was performed by mass spectrometry and was confirmed by molecular methods. susceptibility to antimicrobials was studied by the vitek-2 system. a 94% correlation of both identification methods was found. multidrug resistant acinetobacter baumannii was the predominant genomic species (92.6%) in hospital-acquired in ... | 2015 | 25576416 |
| substitutions of ser83leu in gyra and ser80leu in parc associated with quinolone resistance in acinetobacter pittii. | to investigate the prevalence and the mechanism of quinolone-resistant acinetobacter pittii, 634 acinetobacter calcoaceticus-acinetobacter baumannii complex isolates were collected throughout zhejiang province. identification of isolates was conducted by matrix-assisted laser desorption ionization/time of flight mass spectrometry (maldi-tof ms), blaoxa-51-like gene, and partial rna polymerase β-subunit (rpob) amplification. twenty-seven isolates of a. pittii were identified. among the 634 isolat ... | 2015 | 25514581 |
| association of class 1 and 2 integrons with multidrug-resistant acinetobacter baumannii international clones and acinetobacter nosocomialis isolates. | the acinetobacter baumannii clonal complex 113/79 (cc113/79) and class 2 integrons predominate in latin america; a relationship between these characteristics was explored. the presence of integrases was determined in successive hospital acinetobacter isolates (163 a. baumannii isolates and 72 acinetobacter nosocomialis isolates). most isolates had integrons, but class 1 and 2 integrons were present significantly more often in cc109/1 and cc113/79, respectively. the high prevalence of cc113/79 in ... | 2015 | 25348522 |
| isolation and characterization of two plasmids in a clinical acinetobacter nosocomialis strain. | acinetobacter species are recognised as important nosocomial pathogens that have become a major cause of invasive opportunistic infections in hospitalised patients. their clinical significance is largely due to the rapid development of antimicrobial resistance among strains. the development of antibiotic resistance among bacterial strains occurs frequently by the acquisition of resistance genes by gene transfer systems such as bacterial plasmids. | 2014 | 25326196 |
| influence of acb complex genospecies on clinical outcomes in a u.s. hospital with high rates of multidrug resistance. | bacteria within the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex commonly cause nosocomial infection and are often multidrug resistant. advances in genospecies typing allow for speciation within the acb complex; however, little is known about the effect of genospecies on patient outcomes. | 2015 | 25246361 |
| rapid identification of acinetobacter baumannii, acinetobacter nosocomialis and acinetobacter pittii with a multiplex pcr assay. | acinetobacter baumannii, acinetobacter nosocomialis and acinetobacter pittii are clinically relevant members of the acinetobacter calcoaceticus-a. baumannii (acb) complex and important nosocomial pathogens. these three species are genetically closely related and phenotypically similar; however, they differ in their epidemiology, antibiotic resistance and pathogenicity. in this study, we investigated the use of a multiplex pcr-based assay designed to detect internal fragments of the 16s-23s rrna ... | 2014 | 24965800 |
| a 16-year prospective study of community-onset bacteremic acinetobacter pneumonia: low mortality with appropriate initial empirical antibiotic protocols. | the genus acinetobacter, well known as a nosocomial pathogen, can also cause severe community-onset pneumonia. previous small case series have suggested fulminant disease and a pooled hospital mortality of > 60%. | 2014 | 24901292 |
| maldi-tof ms and chemometric based identification of the acinetobacter calcoaceticus-acinetobacter baumannii complex species. | maldi-tof ms is becoming the technique of choice for rapid bacterial identification at species level in routine diagnostics. however, some drawbacks concerning the identification of closely related species such as those belonging to the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex lead to high rates of misidentifications. in this work we successfully developed an approach that combines maldi-tof ms and chemometric tools to discriminate the six acb complex species (a. baumann ... | 2014 | 24877727 |
| comparative in vitro activity of sitafloxacin against bacteremic isolates of carbapenem resistant acinetobacter baumannii complex. | the emergence of carbapenem-resistant acinetobacter baumannii (crab) complex has posed a great challenge to clinicians worldwide. sitafloxacin has been shown to have in vitro activity against pathogens resistant to other fluoroquinolones. however, data comparing the anti-crab activity of sitafloxacin with that of other antimicrobial agents are limited. | 2015 | 24662017 |
| worldwide dissemination of acquired carbapenem-hydrolysing class d β-lactamases in acinetobacter spp. other than acinetobacter baumannii. | the aim of this study was to identify acquired oxa-type carbapenemases in acinetobacter spp. other than acinetobacter baumannii. from a total of 453 carbapenem-susceptible and -resistant acinetobacter isolates collected worldwide, 23 were positive for blaoxa genes by multiplex pcr. these isolates were identified as acinetobacter pittii (n=18), acinetobacter nosocomialis (n=2), acinetobacter junii (n=1) and acinetobacter genomic species 14tu/13bj (n=2). the blaoxa genes and associated insertion s ... | 2014 | 24612983 |
| characterization of a novel plasmid type and various genetic contexts of bla oxa-58 in acinetobacter spp. from multiple cities in china. | several studies have described the epidemiological distribution of blaoxa-58-harboring acinetobacter baumannii in china. however, there is limited data concerning the replicon types of blaoxa-58-carrying plasmids and the genetic context surrounding blaoxa-58 in acinetobacter spp. in china. | 2014 | 24400107 |
| improvement of maldi-tof ms profiling for the differentiation of species within the acinetobacter calcoaceticus-acinetobacter baumannii complex. | maldi-tof ms is currently becoming the method of choice for rapid identification of bacterial species in routine diagnostics. yet, this method suffers from the inability to differentiate reliably between some closely related bacterial species including those of the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex, namely a. baumannii and acinetobacter nosocomialis. in the present study, we evaluated a protocol which was different from that used in the bruker daltonics identifica ... | 2013 | 24054697 |
| increasing prevalence of blaoxa-23-carrying acinetobacter baumannii and the emergence of blaoxa-182-carrying acinetobacter nosocomialis in korea. | carbapenem-resistant acinetobacter spp. have been increasingly reported worldwide with the production of oxa-type carbapenemases as the main mechanism of carbapenem resistance. the prevalent bla(oxa) genes are known to vary significantly depending on time and place of isolation. we investigated the prevalence of bla(oxa) genes by pcr in acinetobacter spp. isolated in korea. among a total of 336 isolates collected from hospital a from 2002 to 2011, the overall proportion of bla(oxa)-23-like, isab ... | 2013 | 23891219 |
| molecular genotyping of acinetobacter spp. isolated in arizona, usa, using multilocus pcr and mass spectrometry. | acinetobacter spp. are a diverse group of gram-negative bacteria frequently implicated in nosocomial infections. genotypic methods have been instrumental in studying acinetobacter, but few offer high resolution, rapid turnaround time, technical ease and high inter-laboratory reproducibility, which has hampered understanding of disease incidence, transmission patterns and diversity within this genus. here, we further evaluated multilocus pcr electrospray ionization/mass spectrometry (pcr/esi-ms), ... | 2013 | 23741021 |
| risk factors for imipenem-nonsusceptible acinetobacter nosocomialis bloodstream infection. | the emergence of imipenem-nonsusceptible (ins) acinetobacter baumannii complex has had a great impact on healthcare systems worldwide. understanding the risk factors related to ins infection is useful for infection control. the risk factors for ins a. baumannii have been well documented; however, the risk factors related to ins acinetobacter nosocomialis infection lack documentation. the purpose of this study was to identify the risk factors associated with ins a. nosocomialis bacteremia. | 2014 | 23642729 |
| imported and intensive care unit-born acinetobacter baumannii clonal complexes: one-year prospective cohort study in intensive care patients. | the main objective of this study was to assess the frequency and possible sources of colonization and infection by acinetobacter in the intensive care unit (icu) of a university hospital in rio de janeiro, brazil, and characterize the isolates for relatedness to internationally and locally disseminated lineages. patients consecutively admitted to the icu from april 2007 to april 2008 were screened for colonization and infection. species were identified by rpob sequencing. the presence of acquire ... | 2013 | 23336529 |
| therapeutic options for acinetobacter baumannii infections: an update. | although the clinical importance of acinetobacter pittii and acinetobacter nosocomialis has increased in the nosocomial setting in the last decade, infections caused by acinetobacter baumannii still have the highest clinical relevance. the most important features of this latter species are the ability to persist in the hospital environment and the multi-drug, extended-drug or pandrug resistance they may present which compromises the treatment of infections caused by this microorganism. | 2012 | 23035697 |
| molecular epidemiology of acinetobacter baumannii and acinetobacter nosocomialis in germany over a 5-year period (2005-2009). | to investigate the species distribution within the acinetobacter calcoaceticus-acinetobacter baumannii complex and the molecular epidemiology of a. baumannii and acinetobacter nosocomialis, 376 acinetobacter isolates were collected prospectively from hospitalized patients at 15 medical centres in germany during three surveillance studies conducted over a 5-year period. species identification was performed by molecular methods. imipenem minimum inhibitory concentrations (mic) were determined by b ... | 2013 | 23034071 |
| bacteremic nosocomial pneumonia caused by acinetobacter baumannii and acinetobacter nosocomialis: a single or two distinct clinical entities? | the phenotypically indistinguishable acinetobacter baumannii and acinetobacter nosocomialis have become leading pathogens causing nosocomial pneumonia in critically ill patients. a. baumannii and a. nosocomialis nosocomial pneumonias were grouped as a single clinical entity previously. this study aimed to determine whether they are the same or a different clinical entity. a total of 121 patients with a. baumannii and 131 with a. nosocomialis bacteremic nosocomial pneumonia were included during a ... | 2013 | 22967204 |
| evaluation of the effect of appropriate antimicrobial therapy on mortality associated with acinetobacter nosocomialis bacteraemia. | appropriate antimicrobial therapy is effective for severe infections caused by acinetobacter baumannii, but efficacy for other acinetobacter species remains to be established. the current study was designed to determine whether appropriate antimicrobial therapy reduces the mortality of patients with acinetobacter nosocomialis bacteraemia. a 9-year retrospective study of 266 patients with monomicrobial a. nosocomialis bacteraemia was conducted at a large teaching hospital in taiwan. multivariable ... | 2013 | 22776466 |
| molecular characterization of carbapenem-non-susceptible acinetobacter spp. in japan: predominance of multidrug-resistant acinetobacter baumannii clonal complex 92 and imp-type metallo-β-lactamase-producing non-baumannii acinetobacter species. | we conducted an epidemiological study concerning carbapenem-non-susceptible clinical isolates of acinetobacter spp. in japan by molecular procedures including carbapenemase gene identification and amplified ribosomal dna restriction analysis. among 598 clinically isolated acinetobacter spp. in 2007, 27 (4.5%) were non-susceptible to carbapenems. most carbapenem-non-susceptible acinetobacter baumannii (13/14) belonged to clonal complex (cc) 92, harbored bla (oxa-51-like) genes, including novel bl ... | 2012 | 22327516 |
| efficacy of six frog skin-derived antimicrobial peptides against colistin-resistant strains of the acinetobacter baumannii group. | the emergence of acinetobacter sp. strains resistant to all antibacterial agents including colistin necessitates the development of new types of antimicrobial agents. six cationic α-helical frog skin-derived peptides (cpf-am1, pgla-am1, b2rp-era, [e4k]alyteserin-1c, [d4k]b2rp and [g4k]xt-7) were selected for this study on the basis of potent growth-inhibitory activity against gram-negative bacteria and low haemolytic activity against human erythrocytes. all peptides were active against a range o ... | 2012 | 22326566 |
| regional dissemination of acinetobacter species harbouring metallo-β-lactamase genes in japan. | metallo-β-lactamase (mbl) producers have been reported among the various acinetobacter species worldwide. in this study, the epidemiology and molecular characteristics of carbapenemase-encoding genes and mobile elements were studied to analyse the regional dissemination of mbl genes in acinetobacter species. from january 2001 to december 2006, 48 acinetobacter isolates harbouring mbl genes identified from five hospitals in kyoto and shiga prefecture, japan were collected and analysed. the partia ... | 2013 | 23009350 |
| acinetobacter pittii and acinetobacter nosocomialis among clinical isolates of the acinetobacter calcoaceticus-baumannii complex in sichuan, china. | among 82 clinical isolates of the acinetobacter calcoaceticus-baumannii complex recovered in 13 hospitals of sichuan, china, in 2011, 13 were acinetobacter pittii and 2 were acinetobacter nosocomialis. multilocus sequence typing revealed a novel sequence type (st) of a. nosocomialis and 7 novel sts of a. pittii. most isolates were hospital-acquired and colonized in the respiratory tract, while 6 cases with pneumonia due to a. pittii were identified. this study provided a snapshot of the local in ... | 2013 | 23639796 |
| emergence of carbapenemase-producing gram-negative bacteria in saint petersburg, russia. | the emergence and spread of carbapenemase-producing gram-negative bacteria represents a serious public health concern. here we show that of 477 gram-negative isolates collected from 18 hospitals between november 2011 and february 2013 in saint petersburg (russia), minimum inhibitory concentrations (mics) were greater than the european committee on antimicrobial susceptibility testing (eucast) epidemiological cut-off value of at least one carbapenem antibiotic in 101 isolates (21.2%). the bla(ndm ... | 2014 | 25059447 |
| characterization of tet(y)-carrying lowgc plasmids exogenously captured from cow manure at a conventional dairy farm. | manure from dairy farms has been shown to contain diverse tetracycline resistance genes that are transferable to soil. here, we focus on conjugative plasmids that may spread tetracycline resistance at a conventional dairy farm. we performed exogenous plasmid isolation from cattle feces using chlortetracycline for transconjugant selection. the transconjugants obtained harbored lowgc-type plasmids and tet(y). a representative plasmid (pfk2-7) was fully sequenced and this was compared with previous ... | 2016 | 27083193 |
| clinical outcomes of hospital-acquired infection with acinetobacter nosocomialis and acinetobacter pittii. | the role of acinetobacter nosocomialis and acinetobacter pittii, which belong to the a. calcoaceticus-a. baumannii complex, in hospital-acquired infections is increasingly recognized. here we describe a retrospective cohort study of hospital-acquired a. calcoaceticus-a. baumannii complex infections at a university hospital in thailand. a total of 222 unique cases were identified between january 2010 and december 2011. the genomospecies of the a. calcoaceticus-a. baumannii complex isolates were c ... | 2014 | 24820079 |
| molecular characterisation of carbapenemases in urban pigeon droppings in france and algeria. | the main objective of this study was to detect the presence of carbapenemase-encoding genes in stool samples of urban pigeons. | 2017 | 28487209 |
| sensitivity and specificity of matrix-associated laser desorption/ionization - time of flight mass spectrometry (maldi-tof ms) in discrimination at species level for acinetobacter bacteremia. | we aimed to establish the role of maldi-tof ms on species discrimination of phenotypically indistinguishable a. baumannii, a. pittii and a. nosocomialis. compared to multiplex pcr, the gold standard, maldi-tof ms yielded a high sensitivity for a. baumannii (97.9%) and specificity for a. pittii (98.9%) and a. nosocomialis (100%). | 2017 | 28669800 |
| development and evaluation of a core genome multilocus typing scheme for whole-genome sequence-based typing of acinetobacter baumannii. | we have employed whole genome sequencing to define and evaluate a core genome multilocus sequence typing (cgmlst) scheme for acinetobacter baumannii. to define a core genome we downloaded a total of 1,573 putative a. baumannii genomes from ncbi as well as representative isolates belonging to the eight previously described international a. baumannii clonal lineages. the core genome was then employed against a total of fifty-three carbapenem-resistant a. baumannii isolates that were previously typ ... | 2017 | 28594944 |
| pathogenic acinetobacter species have a functional type i secretion system and contact-dependent inhibition systems. | pathogenic acinetobacter species, including acinetobacter baumannii and acinetobacter nosocomialis, are opportunistic human pathogens of increasing relevance worldwide. although their mechanisms of drug resistance are well studied, the virulence factors that govern acinetobacter pathogenesis are incompletely characterized. here we define the complete secretome of a. nosocomialis strain m2 in minimal medium and demonstrate that pathogenic acinetobacter species produce both a functional type i sec ... | 2017 | 28373284 |
| seasonal and temperature-associated increase in community-onset acinetobacter baumannii complex colonization or infection. | identifying the trends in community-onset acinetobacter baumannii complex isolation and diversity according to temperature could help provide insight into the behavior of the a. baumannii complex. we performed a retrospective analysis of a. baumannii complex (acinetobacter baumannii, acinetobacter nosocomialis, acinetobacter pittii, and acinetobacter calcoaceticus) isolates obtained from patients at a korean community hospital from 2006 to 2015 with reference to seasonal temperatures. the incide ... | 2018 | 29401563 |
| genomic and biochemical characterization of acinetobacter podophage petty reveals a novel lysis mechanism and tail-associated depolymerase activity. | the increased prevalence of drug-resistant, nosocomial acinetobacter infections, particularly from pathogenic members of the acinetobacter calcoaceticus-baumannii complex, necessitates the exploration of novel treatments such as phage therapy. in the present study, we characterize phage petty, a novel podophage that infects multidrug-resistant acinetobacter nosocomialis and acinetobacter baumannii genome analysis reveals that phage petty is a 40,431bp ϕkmv-like phage, with a coding density of 92 ... | 2018 | 29298884 |
| defining the interaction of the protease cpaa with its type ii secretion chaperone cpab and its contribution to virulence in acinetobacter species. | acinetobacter baumannii, acinetobacter nosocomialis, and acinetobacter pittii are a frequent cause of multidrug-resistant, healthcare-associated infections. our previous work demonstrated that a. nosocomialis m2 possesses a functional type ii secretion system (t2ss) that is required for full virulence. further, we identified the metallo-endopeptidase cpaa, which has been shown previously to cleave human factor v and deregulate blood coagulation, as the most abundant type ii secreted effector pro ... | 2017 | 28982978 |
| structural and functional investigation of fdhc from acinetobacter nosocomialis: a sugar n-acyltransferase belonging to the gnat superfamily. | enzymes belonging to the gnat superfamily are widely distributed in nature where they play key roles in the transfer of acyl groups from acyl-coas to primary amine acceptors. the amine acceptors run the gamut from histones to aminoglycoside antibiotics to small molecules such as serotonin. whereas those family members that function on histones have been extensively studied, the gnat enzymes that employ nucleotide-linked sugars as their substrates have not been well characterized. indeed, though ... | 2016 | 27404806 |
| outer membrane protein a plays a role in pathogenesis of acinetobacter nosocomialis. | acinetobacter nosocomialis is an important nosocomial pathogen that causes a variety of human infections. however, the specific virulence factors of this microorganism have not yet been determined. we investigated the role of outer membrane protein a (ompa) in the pathogenesis of a. nosocomialis. a δompa mutant of the a. nosocomialis atcc 17903(t) strain was constructed using markerless gene deletion. the δompa mutant displayed reduced biofilm formation in polystyrene tubes and reduced adherence ... | 2016 | 26759990 |
| role of luxir homologue anoir in acinetobacter nosocomialis and the effect of virstatin on the expression of anor gene. | quorum sensing is a process of cell-to-cell communication in which bacteria produce autoinducers as signaling molecules to sense cell density and coordinate gene expression. in the present study, a luxi-type synthase, anoi, and a luxr-type regulator, anor, were identified in acinetobacter nosocomialis, an important nosocomial pathogen, by sequence analysis of the bacterial genome. we found that n-(3-hydroxy-dodecanoyl)-l-homoserine lactone (oh-ddhl) is a quorum-sensing signal in a. nosocomialis. ... | 2015 | 25975610 |
| acinetobacter nosocomialis secretes outer membrane vesicles that induce epithelial cell death and host inflammatory responses. | acinetobacter nosocomialis is an important nosocomial pathogen that causes a variety of opportunistic infections; however, pathogenesis of this microorganism has not yet been characterized. the aim of this study was to investigate the secretion of outer membrane vesicles (omvs) from a. nosocomialis and to determine their cytotoxic effects and their ability to induce inflammatory responses both in vitro and in vivo by using human epithelial hep-2 cells and a mouse model, respectively. a. nosocomi ... | 2015 | 25778390 |
| molecular epidemiology of carbapenem non-susceptible acinetobacter nosocomialis in a medical center in taiwan. | the mechanism by which carbapenem non-susceptible acinetobacter nosocomialis (cnsan) is disseminated is rarely described in the literature. in this study, we delineated the molecular epidemiology of cnsan isolated from patients in a medical center in taiwan. fifty-four non-duplicate bloodstream isolates of cnsan were collected at the taipei veterans general hospital between 2001 and 2007. pulsed-field gel electrophoresis (pfge) was performed to determine their clonal relationship. carbapenem-res ... | 2015 | 25724091 |
| risk factors and clinical outcome of sulbactam nonsusceptibility in monomicrobial acinetobacter nosocomialis bacteremia. | sulbactam is an effective antimicrobial agent against multidrug-resistant acinetobacter spp. this retrospective study evaluated the risk factors of sulbactam nonsusceptibility (sns) in monomicrobial acinetobacter nosocomialis bacteremia and its related outcome. | 2016 | 25081987 |
| dissemination of acinetobacter nosocomialis clone among critically ill patients and the environment. | we examined the environmental dissemination of acinetobacter nosocomialis multilocus sequence typing clonal complex 260/71 in rio de janeiro, brazil, including water from a dam and food samples. the increasing use of sequence based methods has demonstrated a large, previously unpredicted, dissemination of bacteria that may serve as opportunistic pathogens. | 2013 | 23698521 |
| clinical characteristics and prognostic factors of acinetobacter nosocomialis bacteraemia in patients with solid tumours. | the clinical characteristics and risk factors for 28-day mortality in 120 patients with solid tumours with acinetobacter nosocomialis bacteraemia were retrospectively analysed. eighty-one patients (67.5%) had advanced-stage cancer. most of the bacteraemia (37.5%) did not have an identified source. the bacteraemia episodes developed at a median of 15 days after hospitalization, and most during a non-neutropenic period (97.5%). although only half of the patients received appropriate antimicrobial ... | 2012 | 22712825 |