| phylogenetic analysis of acinetobacter strains based on the nucleotide sequences of gyrb genes and on the amino acid sequences of their products. | partial nucleotide sequences of the gyrb genes (dna gyrase b subunit genes) of 15 acinetobacter strains, including the type and reference strains of genomic species 1 to 12 (a. calcoaceticus [genomic species 1], a. baumannii [genomic species 2], acinetobacter genomic species 3, a. haemolyticus [genomic species 4], a. junii [genomic species 5], acinetobacter genomic species 6, a. johnsonii [genomic species 7], a. lwoffii [genomic species 8], acinetobacter genomic species 9, acinetobacter genomic ... | 1996 | 8934907 |
| acinetobacter bereziniae sp. nov. and acinetobacter guillouiae sp. nov., to accommodate acinetobacter genomic species 10 and 11, respectively. | acinetobacter genospecies (genomic species) 10 and 11 were described by bouvet and grimont in 1986 on the basis of dna-dna reassociation studies and comprehensive phenotypic analysis. in the present study, the names acinetobacter bereziniae sp. nov. and acinetobacter guillouiae sp. nov., respectively, are proposed for these genomic species based on the congruence of results of polyphasic analysis of 33 strains (16 and 17 strains of genomic species 10 and 11, respectively). all strains were inves ... | 2010 | 19661501 |
| bacteremia due to acinetobacter genomic species 10. | six patients with acinetobacter genomic species 10 bacteremia were identified. the clinical features of the patients, phenotypic and genotypic identifications, antimicrobial susceptibilities, and genes flanking isaba1 of the bacteria were described. the results revealed that this bacterium is a potentially lethal pathogen that can cause health care-associated infections in debilitated patients. | 2010 | 19955266 |
| characteristics of clinical isolates of acinetobacter genomospecies 10 carrying two different metallo-beta-lactamases. | acquired metallo-beta-lactamase (mbl) production is an important mechanism of carbapenem resistance. to our knowledge, carriage of two different mbls has not been described previously in acinetobacter spp. we present the characteristics of five acinetobacter isolates carrying two different mbl genes. the species of all five acinetobacter isolates with two different mbl genes were acinetobacter genomospecies 10, and bla(imp-1), bla(vim-2) and bla(sim-1) may coexist in this species. minimum inhibi ... | 2010 | 20599361 |
| Characteristics of carbapenem-resistant Acinetobacter spp. other than Acinetobacter baumannii in South Korea. | Although many studies have been performed on carbapenem-resistant Acinetobacter baumannii, only a few studies have addressed carbapenem resistance in Acinetobacter spp. other than A. baumannii (non-baumannii Acinetobacter). Amongst 287 Acinetobacter spp. isolates from patients with bacteraemia in a South Korean hospital collected between 2003 and 2010, 160 (55.7%) were non-baumannii Acinetobacter spp. Antimicrobial susceptibility testing was performed and the effect of efflux pump inhibitors was ... | 2012 | 21996405 |
| characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in india and pakistan. | the blandm-1 gene is associated with extensive drug resistance in gram-negative bacteria. this probably spread to enterobacteriaceae from acinetobacter spp., and we characterized plasmids associated with blandm-1 in acinetobacter spp. to gain insight into their role in this dissemination. four clinical ndm-1-producing acinetobacter species strains from india and pakistan were investigated. a plasmid harboring blandm-1, pndm-40-1, was characterized by whole-genome sequencing of acinetobacter bere ... | 2015 | 25421466 |
| development and evaluation of species-specific pcr for detection of nine acinetobacter species. | molecular methods have the potential to improve the speed and accuracy of acinetobacter species identification in clinical settings. the goal of this study is to develop species-specific pcr assays based on differences in the rna polymerase beta-subunit gene (rpob) to detect nine commonly isolated acinetobacter species including acinetobacter baumannii, acinetobacter calcoaceticus, acinetobacter pittii, acinetobacter nosocomialis, acinetobacter lwoffii, acinetobacter ursingii, acinetobacter bere ... | 2016 | 27312551 |
| antimicrobial resistance and resistance genes in aerobic bacteria isolated from pork at slaughter. | the aim of this study was to investigate the phenotypic and genotypic antimicrobial resistance, integrons, and transferability of resistance markers in 243 aerobic bacteria recovered from pork at slaughter in the people's republic of china. the organisms belonged to 22 genera of gram-negative bacteria (92.2%) and gram-positive bacteria (7.8%). high levels of resistance were detected to tetracycline, trimethoprim-sulfamethoxazole, and ampicillin (36.2 to 54.3%), and lower levels were detected to ... | 2016 | 27052863 |
| imported and intensive care unit-born acinetobacter baumannii clonal complexes: one-year prospective cohort study in intensive care patients. | the main objective of this study was to assess the frequency and possible sources of colonization and infection by acinetobacter in the intensive care unit (icu) of a university hospital in rio de janeiro, brazil, and characterize the isolates for relatedness to internationally and locally disseminated lineages. patients consecutively admitted to the icu from april 2007 to april 2008 were screened for colonization and infection. species were identified by rpob sequencing. the presence of acquire ... | 2013 | 23336529 |
| unusual features of the sequences of copies of the 16s-23s rrna internal transcribed spacer regions of acinetobacter bereziniae, acinetobacter guillouiae and acinetobacter baylyi arise from horizontal gene transfer events. | the highly variable nature of the internal transcribed spacer region (its) has been claimed to represent an ideal target for designing species-specific probes/primers capable of differentiating between closely related acinetobacter species. however, several acinetobacter species contain multiple its copies of variable lengths, and these include acinetobacter bereziniae, acinetobacter guillouiae and acinetobacter baylyi. this study shows these length variations result from inter-genomic insertion ... | 2015 | 25505188 |
| acinetobacter larvae sp. nov., isolated from the larval gut of omphisa fuscidentalis. | a gram-stain-negative, non-spore-forming, non-motile, and aerobic coccobacilli shaped strain, designated brtc-1t, was isolated from the gut of omphisa fuscidentalis hampson, which is a larva of a moth and was collected from xishuangbanna dai autonomous prefecture in china. the isolate was found to grow at nacl concentrations of 0-5 % (w/v) (optimum: 0 %), 10-45 ℃ (optimum: 30-35 ℃) and ph 5.0-9.0 (optimum: 6.0) on tsa. analysis of the 16s rrna gene sequence indicated that the isolate belongs to ... | 2016 | 27902257 |
| draft genome sequence of acinetobacter bereziniae hpc229, a carbapenem-resistant clinical strain from argentina harboring blandm-1. | we report here the draft genome sequence of an ndm-1-producing acinetobacter bereziniae clinical strain, hpc229. this strain harbors both plasmid and chromosomal resistance determinants toward different β-lactams and aminoglycosides as well as several types of multidrug efflux pumps, most likely representing an adaptation strategy for survival under different environments. | 2016 | 26966220 |
| extending the reservoir of bla imp-5: the emerging pathogen acinetobacter bereziniae. | acinetobacter bereziniae clinical relevance is starting to be recognized; however, very few descriptions of its carbapenem resistance currently exist. here we characterize two carbapenem-resistant a. bereziniae isolates. | 2015 | 26439605 |
| complete sequence of a bla(ndm-1)-harboring plasmid in an acinetobacter bereziniae clinical strain isolated in argentina. | | 2015 | 26248354 |
| detection of an ndm-1-producing acinetobacter bereziniae strain in brazil. | | 2015 | 27873666 |
| effects of psychrotrophic bacteria, serratia liquefaciens and acinetobacter genomospecies 10 on yogurt quality. | the aim of this study was to evaluate the effect of proteolytic (serratia liquefaciens, match %: 99.39) or lipolytic (acinetobacter genomospecies 10, match %: 99.90) psychrotrophic bacteria (bacterial counts, analysis of free fatty acids (ffa) and analysis of free amino acids) on the microbial and chemical properties (yogurt composition), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) of yogurt during storage. yogurts were prepared with raw milk preinoculated with each ... | 2014 | 26761293 |
| multiplex pcr to detect the genes encoding naturally occurring oxacillinases in acinetobacter spp. | bacteria of the genus acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. species identification is difficult and there is a need for simple molecular methods to differentiate between the species. naturally occurring oxacillinase genes (blaoxa) have been identified in several acinetobacter species and their detection by pcr can aid in species identification. the aim of this study was to develop a multiplex pcr to identify intrinsic blao ... | 2014 | 24288029 |
| insertion sequence is18 mediates overexpression of blaoxa-257 in a carbapenem-resistant acinetobacter bereziniae isolate. | | 2014 | 23934740 |
| regional dissemination of acinetobacter species harbouring metallo-β-lactamase genes in japan. | metallo-β-lactamase (mbl) producers have been reported among the various acinetobacter species worldwide. in this study, the epidemiology and molecular characteristics of carbapenemase-encoding genes and mobile elements were studied to analyse the regional dissemination of mbl genes in acinetobacter species. from january 2001 to december 2006, 48 acinetobacter isolates harbouring mbl genes identified from five hospitals in kyoto and shiga prefecture, japan were collected and analysed. the partia ... | 2013 | 23009350 |
| biochemical and genetic characterization of carbapenem-hydrolyzing β-lactamase oxa-229 from acinetobacter bereziniae. | acinetobacter bereziniae (formerly acinetobacter genomospecies 10) isolate nec was recovered from a skin sample of a patient hospitalized in paris, france. it was resistant to penicillins, penicillin-inhibitor combinations, and carbapenems. cloning and expression in escherichia coli identified the carbapenem-hydrolyzing class d β-lactamase oxa-229, which is weakly related to other oxacillinases (66% amino acid identity with the closest oxacillinase, oxa-58). it hydrolyzed penicillins, oxacillin, ... | 2012 | 22508298 |
| mechanistic insights into dimethylsulfoniopropionate lyase dddy, a new member of the cupin superfamily. | the marine osmolyte dimethylsulfoniopropionate (dmsp) is one of earth's most abundant organosulfur molecules. bacterial dmsp lyases cleave dmsp, producing acrylate and dimethyl sulfide (dms), a climate-active gas with roles in global sulfur cycling and atmospheric chemistry. dddy is the only known periplasmic dmsp lyase and is present in β-, γ-, δ- and ε-proteobacteria. unlike other known dmsp lyases, dddy has not been classified into a protein superfamily, and its structure and catalytic mechan ... | 2017 | 29106934 |