| survey of cyclopids (crustacea, copepoda) in brazil and preliminary screening of their potential as dengue vector predators. | cyclopid copepods are known to be good mosquito controllers, specially as regards the larvae of the dengue vectors aedes aegypti and ae. albopictus. | 1997 | 9515258 |
| isolation and characterization of diverse halobenzoate-degrading denitrifying bacteria from soils and sediments. | denitrifying bacteria capable of degrading halobenzoates were isolated from various geographical and ecological sites. the strains were isolated after initial enrichment on one of the monofluoro-, monochloro-, or monobromo-benzoate isomers with nitrate as an electron acceptor, yielding a total of 33 strains isolated from the different halobenzoate-utilizing enrichment cultures. each isolate could grow on the selected halobenzoate with nitrate as the terminal electron acceptor. the isolates obtai ... | 2000 | 10919805 |
| nitrogen-fixing nodules with ensifer adhaerens harboring rhizobium tropici symbiotic plasmids. | ensifer adhaerens is a soil bacterium that attaches to other bacteria and may cause lysis of these other bacteria. based on the sequence of its small-subunit rrna gene, e. adhaerens is related to sinorhizobium spp. e. adhaerens atcc 33499 did not nodulate phaseolus vulgaris (bean) or leucaena leucocephala, but with symbiotic plasmids from rhizobium tropici cfn299 it formed nitrogen-fixing nodules on both hosts. the nodule isolates were identified as e. adhaerens isolates by growth on selective m ... | 2001 | 11425750 |
| sinorhizobium morelense sp. nov., a leucaena leucocephala-associated bacterium that is highly resistant to multiple antibiotics. | sinorhizobium morelense sp. nov. is described to designate a group of bacteria isolated from root nodules of leucaena leucocephala. s. morelense shows 98% 16s rrna gene sequence similarity to some sinorhizobium species and to ensifer adhaerens. this novel species is distinguished from other sinorhizobium species and from e. adhaerens by dna-dna hybridization, 165 rrna gene restriction fragments and sequence and some distinctive phenotypic features. strains of this species are highly resistant to ... | 2002 | 12361275 |
| predatory prokaryotes: an emerging research opportunity. | predatory prokaryotes have evolved a unique strategy of obtaining energy and biosynthetic materials from their surroundings: acquiring them from other living bacterial cells. these types of microbes have been found in a diverse variety of environments, and may play an important role in modulating microbial population structure and dynamics, as has been hypothesized for marine viruses and possibly protists. only one genus of predatory bacterium, bdellovibrio, has been extensively described and st ... | 2002 | 12432957 |
| description of new ensifer strains from nodules and proposal to transfer ensifer adhaerens casida 1982 to sinorhizobium as sinorhizobium adhaerens comb. nov. request for an opinion. | a group of four diverse rhizobial isolates and two soil isolates that are highly related to ensifer adhaerens were characterized by a polyphasic approach. on the basis of dna-dna hybridizations and phenotypic features, these strains cannot be distinguished clearly form ensifer adhaerens, a soil bacterium that was described in 1982, mainly on the basis of phenotypic characteristics. phylogenetically, ensifer and sinorhizobium form a single group in the 16s rdna dendrogram of the alpha-proteobacte ... | 2003 | 12892151 |
| changing concepts in the systematics of bacterial nitrogen-fixing legume symbionts. | as of february 2003, bacteria that form nitrogen-fixing symbiotic associations with legumes have been confirmed in 44 species of 12 genera. phylogenies of these taxa containing legume symbionts based on the comparative analysis of 16s rdna sequences show that they are not clustered in one lineage but are distributed in the classes alphaproteobacteria and betaproteobacteria, and dispersed over the following nine monophyletic groups, being intermingled with other taxa that do not contain legume sy ... | 2003 | 12949698 |
| the genus name ensifer casida 1982 takes priority over sinorhizobium chen et al. 1988, and sinorhizobium morelense wang et al. 2002 is a later synonym of ensifer adhaerens casida 1982. is the combination "sinorhizobium adhaerens" (casida 1982) willems et al. 2003 legitimate? request for an opinion. | the synonymy of the genera ensifer and sinorhizobium was recently reported, but it was proposed that the later-named genus, sinorhizobium, take priority in nomenclature. there is no justification in the international code of nomenclature of bacteria (prokaryotes) for this step; ensifer is the correct name of the genus, with ensifer adhaerens as the type species. species previously allocated to sinorhizobium are here proposed as the new combinations ensifer arboris, ensifer fredii, ensifer kostie ... | 2003 | 14657154 |
| cupin-type phosphoglucose isomerases (cupin-pgis) constitute a novel metal-dependent pgi family representing a convergent line of pgi evolution. | cupin-type phosphoglucose isomerases (cpgis) were identified in some archaeal and bacterial genomes and the respective coding function of cpgi's from the euryarchaeota archaeoglobus fulgidus and methanosarcina mazei, as well as the bacteria salmonella enterica serovar typhimurium and ensifer meliloti, was proven by functional overexpression. these cpgis and the cpgis from pyrococcus and thermococcus spp. represent the cpgi family and were compared with respect to kinetic, inhibitory, thermophili ... | 2005 | 15716432 |
| genetic diversity of benzoyl coenzyme a reductase genes detected in denitrifying isolates and estuarine sediment communities. | benzoyl coenzyme a (benzoyl-coa) reductase is a central enzyme in the anaerobic degradation of organic carbon, which utilizes a common intermediate (benzoyl-coa) in the metabolism of many aromatic compounds. the diversity of benzoyl-coa reductase genes in denitrifying bacterial isolates capable of degrading aromatic compounds and in river and estuarine sediment samples from the arthur kill in new jersey and the chesapeake bay in maryland was investigated. degenerate primers were developed from t ... | 2005 | 15812036 |
| new evidence for bacterial diversity in the ascoma of the ectomycorrhizal fungus tuber borchii vittad. | the microbial community associated with ascocarps of the ectomycorrhizal fungus tuber borchii vittad. was studied by both cultivation and direct extraction of bacterial 16s rrna gene (rdna) sequence approaches. the inner part of six t. borchii ascoma collected in north-central italy was used to establish a bacterial culture collection and to extract the total genomic dna to obtain a library of 16s rdnas representative of the truffle bacterial community. most of the isolates were affiliated to th ... | 2005 | 15927744 |
| ensifer adhaerens predatory activity against other bacteria in soil, as monitored by indirect phage analysis. | an indirect phage analysis procedure was used to detect and follow the activity of the bacterial predator ensifer adhaerens in situ in natural soil. the soil was percolated with an aqueous suspension of washed bacterial host cells so that the e. adhaerens cells naturally present in the soil would multiply in response to the host cells. the natural phage development which ensued against these multiplying e. adhaerens cells in the soil was then monitored by noting plaques which developed when the ... | 1983 | 16346275 |
| study of bacillus subtilis endospores in soil by use of a modified endospore stain. | the schaeffer-fulton endospore stain was modified so that it would stain bacillus subtilis endospores in soil smears. the modified stain differentiated among dormant spores, spores undergoing activation, and spores which had germinated but had not yet shown outgrowth. these differentiations were seen for spores in soil and for pure spore preparations in the laboratory. this stain was used to show reversible b. subtilis spore activation promoted by an ensifer adhaerens-like indigenous bacterium i ... | 1985 | 16346801 |
| genetic diversity of acacia tortilis ssp. raddiana rhizobia in tunisia assessed by 16s and 16s-23s rdna genes analysis. | in order to understand the genetic diversity of acacia tortilis ssp. raddiana-rhizobia in tunisia, isolates from nine geographical locations were obtained and analysed. | 2006 | 16478483 |
| metabolic pathway of xenoestrogenic short ethoxy chain-nonylphenol to nonylphenol by aerobic bacteria, ensifer sp. strain as08 and pseudomonas sp. strain as90. | ensifer sp. strain as08 and pseudomonas sp. strain as90 degrading short ethoxy (eo) chain-nonylphenol (np) [npeo(av2.0) containing np mono- approximately tetraethoxylates (np1eo approximately np4eo); average 2.0 eo units] were isolated by enrichment cultures. both strains grew on np but not on octyl- and nonylphenol polyethoxylates (npeos) (average 10 eo units). growth and degradation of npeo(av2.0) was increased with increased concentrations of yeast extract (0.02-0.5%) in a culture medium. cul ... | 2006 | 16528514 |
| occurrence of rhizobia in the gut of the higher termite nasutitermes nigriceps. | wood-eating termites feed on a diet highly deficient in nitrogen. they must complement their diet with the aid of nitrogen-fixing bacteria. nitrogen fixation in the gut has been demonstrated, but information about nitrogen-fixing bacteria in pure culture is scarce. from the higher termite nasutitermes nigriceps the symbiotic bacterial strain m3a was isolated, which thrives in the hindgut contents. the gram-negative strain exhibited similarities to the species of the genus ensifer (including sino ... | 2007 | 16584862 |
| factors affecting the attachment of rhizospheric bacteria to bean and soybean roots. | the plant rhizosphere is an important soil ecological environment for plant-microorganism interactions, which include colonization by a variety of microorganisms in and around the roots that may result in symbiotic, endophytic, associative, or parasitic relationships within the plant, depending on the type of microorganisms, soil nutrient status, and soil environment. rhizosphere competence may be attributable to the differences in the extent of bacterial attachment to the root surface. we prese ... | 2006 | 16684104 |
| analysis of denitrification genes and comparison of nosz, cnorb and 16s rdna from culturable denitrifying bacteria in potato cropping systems. | bacterial denitrification in agricultural soils is a major source of nitrous oxide, a potent greenhouse gas. this study examined the culturable bacterial population of denitrifiers in arable field soils in potato (solanum tuberosum l.) production and denitrification genes (nir, nor and nos) and 16s rdna in those isolates. enrichments for culturable denitrifiers yielded 31 diverse isolates that were then analysed for denitrification genes. the nitrous oxide reductase (nosz) gene was found in all ... | 2007 | 16793234 |
| xenoestrogenic short ethoxy chain nonylphenol is oxidized by a flavoprotein alcohol dehydrogenase from ensifer sp. strain as08. | the ethoxy chains of short ethoxy chain nonylphenol (npeo(av2.0), containing average 2.0 ethoxy units) were dehydrogenated by cell-free extracts from ensifer sp. strain as08 grown on a basal medium supplemented with npeo(av2.0). the reaction was coupled with the reduction in 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide and phenazine methosulfate. the enzyme (npeo(av2.0) dehydrogenase; npeo-dh) was purified to homogeneity with a yield of 20% and a 56-fold increase in specific activ ... | 2007 | 17131148 |
| sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in qingdao, china. | the taxonomic position of strain lmg 23452(t), which was isolated from coastal sediment from an aquaculture site near qingdao, china, in 2000, was determined. strain lmg 23452(t) comprised gram-negative, non-spore-forming, motile rods and was found to be a halotolerant, aerobic, chemoheterotroph that produces catalase and oxidase. comparative 16s rrna gene sequence analysis revealed that strain lmg 23452(t) shared approximately 89 % sequence similarity with members of the genera devosia, hyphomo ... | 2007 | 17220452 |
| a prototype taxonomic microarray targeting the rpsa housekeeping gene permits species identification within the rhizobial genus ensifer. | to develop a reliable tool for the identification and classification of the different ensifer species, without the need for sequencing, a prototype dna microarray that targets the rpsa housekeeping gene was designed and tested. internal segments of the rpsa gene from 34 reference strains, representing the different ensifer species, were sequenced and the sequences were used to select 44 diagnostic oligonucleotides that served as probes for the identification microarray. both, genomic dna and spe ... | 2007 | 17291704 |
| ensifer mexicanus sp. nov. a new species nodulating acacia angustissima (mill.) kuntze in mexico. | a new lineage of ensifer nodulating the american legume acacia angustissima in the tropical forest of chiapas and morelos, mexico is described. bacteria were identified as ensifer with ssb or nolr specific primers. phylogenetic analysis with partial sequences of the five chromosomal genes gyra, nolr, reca, rpob and rrs revealed that this new lineage is related to african ensifer terangae. the results of total dna-dna hybridization and selected phenotypic tests among the a. angustissima strains a ... | 2007 | 17293074 |
| evidence of horizontal transfer of symbiotic genes from a bradyrhizobium japonicum inoculant strain to indigenous diazotrophs sinorhizobium (ensifer) fredii and bradyrhizobium elkanii in a brazilian savannah soil. | the importance of horizontal gene transfer (hgt) in the evolution and speciation of bacteria has been emphasized; however, most studies have focused on genes clustered in pathogenesis and very few on symbiosis islands. both soybean (glycine max [l.] merrill) and compatible bradyrhizobium japonicum and bradyrhizobium elkanii strains are exotic to brazil and have been massively introduced in the country since the early 1960s, occupying today about 45% of the cropped land. for the past 10 years, ou ... | 2007 | 17308185 |
| multilocus sequence analysis of ensifer and related taxa. | multilocus sequence analysis (mlsa) was performed on representatives of ensifer (including species previously assigned to the genus sinorhizobium) and related taxa. neighbour-joining (nj), maximum-parsimony (mp) and maximum-likelihood (ml) phylogenies of dnak, glta, glna, reca, thrc and 16s rrna genes were compared. the data confirm that the potential for discrimination of ensifer species is greater using mlsa of housekeeping genes than 16s rrna genes. in incongruence-length difference tests, th ... | 2007 | 17329774 |
| molecular diversity of legume root-nodule bacteria in kakadu national park, northern territory, australia. | symbiotic relationships between leguminous plants (family fabaceae) and nodule-forming bacteria in australia native ecosystems remain poorly characterized despite their importance. most studies have focused on temperate parts of the country, where the use of molecular approaches have already revealed the presence of bradyrhizobium, ensifer (formerly sinorhizobium), mesorhizobium and rhizobium genera of legume root-nodule bacteria. we here provide the first molecular characterization of nodulatin ... | 2007 | 17356689 |
| rhizobium cellulosilyticum sp. nov., isolated from sawdust of populus alba. | during a study of polysaccharide-hydrolysing bacteria present in different plant sources, two strains were isolated from pulverized decaying wood of populus alba and classified in the genus rhizobium on basis of their almost complete 16s rrna gene sequences. their closest phylogenetic relatives were rhizobium galegae usda 4128(t) and rhizobium huautlense s02(t), with 98.2 and 98.1 % 16s rrna gene sequence similarity, respectively. reca and atpd sequence analysis showed that these species have le ... | 2007 | 17392217 |
| nitric oxide reductase-targeted real-time pcr quantification of denitrifier populations in soil. | the quantification of denitrifying bacteria is a component in the further understanding of denitrification processes in the environment. real-time pcr primers were designed to target two segments of the denitrifier population (cnorb(p) [pseudomonas mandelii and closely related strains] and cnorb(b) [bosea, bradyrhizobium, and ensifer spp.]) in agricultural soils based on functional cnorb (nitric oxide reductase) gene sequences. total population numbers were measured using 16s rrna gene real-time ... | 2007 | 17449686 |
| phenotypic and genotypic diversity of genista saharae microsymbionts from the infra-arid region of tunisia. | aims: genista saharae, indigenous of sahara, is a spontaneous shrub that plays an important ecological role for the preservation and fertility of poor and eroded soils. this legume has not been examined for its root nodule bacteria. the taxonomic diversity of bacteria from root nodules of g. saharae growing in the infra-arid region of tunisia was investigated. methods and results: a total of 28 bacterial strains isolated from root nodules of g. saharae grown in tunisian soil were characterized u ... | 2007 | 17916130 |
| advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus ensifer (including former sinorhizobium). | there is a need for easy, practical, reliable and robust techniques for the identification and classification of bacterial isolates to the species level as alternatives to 16s rrna gene sequence analysis and dna-dna hybridization. here, we demonstrate that multilocus sequence analysis (mlsa) of housekeeping genes is a valuable alternative technique. an mlsa study of 10 housekeeping genes (atpd, dnak, gap, glna, glta, gyrb, pnp, reca, rpob and thrc) was performed on 34 representatives of the genu ... | 2008 | 18175710 |
| molecular diversity and phylogeny of rhizobia associated with wild legumes native to xinjiang, china. | a total of 111 rhizobial strains were isolated from wild legumes in xinjiang, an isolated region of northwest china. nine genomic species belonging to four genera of rhizobium, mesorhizobium, ensifer, and bradyrhizobium were defined among these strains based on the characterization of amplified 16s ribosomal dna restriction analysis (ardra), restriction fragment length polymorphism (rflp) analysis of 16s-23s rdna intergenic spacers (igs), 16s rrna gene sequencing and multilocus sequence analysis ... | 2008 | 18606521 |
| the genus name sinorhizobium chen et al. 1988 is a later synonym of ensifer casida 1982 and is not conserved over the latter genus name, and the species name 'sinorhizobium adhaerens' is not validly published. opinion 84. | the judicial commission affirms that the genus name sinorhizobium chen et al. 1988 is a later synonym of ensifer casida 1982, and that the former genus name is not conserved over the latter genus name. the species name 'sinorhizobium adhaerens' is not validly published. | 2008 | 18676490 |
| diverse bacteria isolated from root nodules of wild vicia species grown in temperate region of china. | in the present study, a total of 154 bacterial strains isolated from nodules of eighteen vicia species mainly grown in the temperate chinese provinces were characterized by ardra, its pcr-rflp, box-pcr, sequencing of 16s rdna, nodc, nifh, atpd and glnii, and nodulation tests. the results demonstrated that most of the r. leguminosarum strains were effective microsymbionts of the wild vicia species, while genomic species related to rhizobium gallicum, mesorhizobium huakuii, ensifer meliloti and br ... | 2008 | 18704366 |
| diversity of bacteria that nodulate prosopis juliflora in the eastern area of morocco. | a total of 274 bacterial strains were isolated from the root nodules of prosopis juliflora, growing in two arid soils of the eastern area of morocco. a physiological plate screening allowed the selection of 15 strains that could tolerate nacl concentrations between 175 and 500 mm. these were compared with 15 strains chosen from among the ones which did not tolerate high salinity. the diversity of strains was first assessed by rep-pcr amplification fingerprinting using boxa1r and eric primers. an ... | 2008 | 18789623 |
| cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from aminobacter sp. aj110403 and ensifer sp. aj110404 and characterization of the recombinant enzyme. | genes encoding alpha-methylserine hydroxymethyltransferase from aminobacter sp. aj110403 and ensifer sp. aj110404 were cloned and expressed in escherichia coli. the purified enzymes were homodimers with a 46-kda subunit and contained 1 mol/mol-subunit of pyridoxal 5'-phosphate. the v(max) of these enzymes catalyzing the conversion of alpha-methyl-l-serine to d-alanine via tetrahydrofolate was 22.1 u/mg (aj110403) and 15.4 u/mg (aj110404). | 2008 | 18997407 |
| effect of poly-3-hydroxybutyrate synthase mutation on the metabolism of ensifer (formerly sinorhizobium) meliloti. | in order to investigate the effect of poly-3-hydroxybutyrate synthase mutation (phbc) on the synthesis of exopolysaccharides (eps) and glycogen, on the symbiotic properties and on the survival under specific conditions of ensifer meliloti (formerly sinorhizobium), a new stable phbc mutant of ensifer meliloti 41 was isolated and characterized.under poly-3-hydroxybutyrate accumulation conditions, the phbc -minus mutant (strain 41003) accumulates more glycogen and less exopolysaccharides as compare ... | 2009 | 19025879 |
| novel genes related to nodulation, secretion systems, and surface structures revealed by a genome draft of rhizobium tropici strain prf 81. | rhizobium tropici is representative of the diversity of tropical rhizobia, besides comprising strains very effective in fixing n(2) in symbiosis with the common bean (phaseolus vulgaris l.). the genome of a brazilian commercial inoculant r. tropici strain (prf 81, =semia 4088), estimated at 7.85 mb, was analyzed through a total of 9,026 shotgun reads, assembled in 1,668 phrap contigs, and covering approximately 30% of the genome. annotation identified 2,135 coding dna sequences (cds), and only 5 ... | 2009 | 19184146 |
| ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with lotus endemic to the canary islands and shows distinctive symbiotic genotypes and host range. | eleven strains were isolated from root nodules of lotus endemic to the canary islands and they belonged to the genus ensifer, a genus never previously described as a symbiont of lotus. according to their 16s rrna and atpd gene sequences, two isolates represented minority genotypes that could belong to previously undescribed ensifer species, but most of the isolates were classified within the species ensifer meliloti. these isolates nodulated lotus lancerottensis, lotus corniculatus and lotus jap ... | 2009 | 19477097 |
| nodulation of sesbania species by rhizobium (agrobacterium) strain irbg74 and other rhizobia. | concatenated sequence analysis with 16s rrna, rpob and fusa genes identified a bacterial strain (irbg74) isolated from root nodules of the aquatic legume sesbania cannabina as a close relative of the plant pathogen rhizobium radiobacter (syn. agrobacterium tumefaciens). however, dna:dna hybridization with r. radiobacter, r. rubi, r. vitis and r. huautlense gave only 44%, 5%, 8% and 8% similarity respectively, suggesting that irbg74 is potentially a new species. additionally, it contained no vir ... | 2009 | 19555380 |
| metabolic capacity of sinorhizobium (ensifer) meliloti strains as determined by phenotype microarray analysis. | sinorhizobium meliloti is a soil bacterium that fixes atmospheric nitrogen in plant roots. the high genetic diversity of its natural populations has been the subject of extensive analysis. recent genomic studies of several isolates revealed a high content of variable genes, suggesting a correspondingly large phenotypic differentiation among strains of s. meliloti. here, using the phenotype microarray (pm) system, hundreds of different growth conditions were tested in order to compare the metabol ... | 2009 | 19561177 |
| multilocus sequence analysis of root nodule isolates from lotus arabicus (senegal), lotus creticus, argyrolobium uniflorum and medicago sativa (tunisia) and description of ensifer numidicus sp. nov. and ensifer garamanticus sp. nov. | nine isolates from argyrolobium uniflorum, lotus creticus , medicago sativa (tunisia) and lotus arabicus (senegal) were analysed by multilocus sequence analysis (mlsa) of five housekeeping genes (reca, atpd, glna, glta and thrc), the 16s rrna gene and the nodulation gene noda. analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, a, b and c, ... | 2010 | 19656922 |
| symbiotic diversity of ensifer meliloti strains recovered from various legume species in tunisia. | ensifer meliloti (formerly sinorhizobium meliloti) was first considered as a specific microsymbiont of medicago, melilotus and trigonella. however, strains of e. meliloti were recovered from root nodules of various legume species and their symbiotic status still remains unclear. here, we further investigate the specificity of these strains. a collection of 47 e. meliloti strains isolated in tunisia from root nodules of medicago truncatula, medicago sativa, medicago ciliaris, medicago laciniata, ... | 2009 | 19665858 |
| nitrite reductase genes in halobenzoate degrading denitrifying bacteria. | abstract diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. nitrite reductase genes were pcr-amplified with degenerate primers (specific to the two different types of respiratory nitrite reductase, nirs and nirk), cloned and sequenced to determine which type of nitrite reductase was present in each isolate and consortium. halobe ... | 2003 | 19719666 |
| ensifer, phyllobacterium and rhizobium species occupy nodules of medicago sativa (alfalfa) and melilotus alba (sweet clover) grown at a canadian site without a history of cultivation. | phage-resistant and -susceptible bacteria from nodules of alfalfa and sweet clover, grown at a site without a known history of cultivation, were identified as diverse genotypes of ensifer, rhizobium and phyllobacterium species based on sequence analysis of ribosomal (16s and 23s rrna) and protein-encoding (atpd and reca) genes, southern hybridization/rflp and a range of phenotypic characteristics. among phage-resistant bacteria, one genotype of rhizobium sp. predominated on alfalfa (frequency ap ... | 2010 | 19875436 |
| biodegradation of diphenylarsinic acid to arsenic acid by novel soil bacteria isolated from contaminated soil. | microorganisms capable of degrading diphenylarsinic acid (dpaa) were enriched from contaminated soil using the soil-charcoal perfusion method. two novel bacterial strains, l2406 and l2413, that can degrade dpaa in a mineral salt medium supplemented with dpaa as the sole carbon source were isolated. based on comparative morphology, physiology, and comparison of the 16s rrna gene sequences, both were presumed to be species closely related to ensifer adhaerens. as the metabolites, phenylarsonic aci ... | 2010 | 19949836 |
| genetic and phenotypic diversity of parathion-degrading bacteria isolated from rice paddy soils. | three parathion-degrading bacteria and eight pairs of bacteria showing syntrophic metabolism of parathion were isolated from rice field soils, and their genetic and phenotypic characteristics were investigated. the three isolates and eight syntrophic pairs were able to utilize parathion as a sole source of carbon and energy, producing p-nitrophenol as the intermediate metabolite during the complete degradation of parathion. analysis of 16s rrna gene sequence indicated that the isolates were rela ... | 2009 | 20075637 |
| identification of sinorhizobium (ensifer) medicae based on a specific genomic sequence unveiled by m13-pcr fingerprinting. | a collection of nodule isolates from medicago polymorpha obtained from southern and central portugal was evaluated by m13-pcr fingerprinting and hierarchical cluster analysis. several genomic clusters were obtained which, by 16s rrna gene sequencing of selected representatives, were shown to be associated with particular taxonomic groups of rhizobia and other soil bacteria. the method provided a clear separation between rhizobia and co-isolated non-symbiotic soil contaminants. ten m13-pcr groups ... | 2009 | 20112226 |
| arsenite oxidation by a facultative chemolithotrophic bacterium sdb1 isolated from mine tailing. | an arsenite (as[iii])-oxidizing bacterium, sdb1, was isolated from mine tailing collected from the sangdong mine area in korea and showed chemolithotrophic growth on as[iii] and co(2) as the respective electron and carbon sources. sdb1 is gram-negative, rod-shaped, and belongs to the sinorhizobium-ensifer branch of alpha-proteobacteria. growth and as[iii] oxidation was enhanced significantly by the presence of yeast extract (0.005%) in minimal salt medium containing 5 mm as[iii]; decreasing the ... | 2010 | 20127460 |
| genetic characterization of root-nodule bacteria associated with acacia salicina and a. stenophylla (mimosaceae) across south-eastern australia. | symbiotic relationships between legumes and nitrogen-fixing soil micro-organisms are of ecological importance in plant communities worldwide. for example, nutrient-poor australian soils are often dominated by shrubby legumes (e.g. species of acacia). however, relatively few studies have quantified patterns of diversity, host-specificity and effectiveness of these ecologically important plant-microbe interactions. in this study, 16s rrna gene sequence and pcr-rflp analyses were used to examine ba ... | 2011 | 20228207 |
| sinorhizobium versus ensifer: may a taxonomy subcommittee of the icsp contradict the judicial commission? | in response to a request for an opinion, the judicial commission (opinion 84) confirmed the earlier synonym, ensifer, as the correct name for the genus better known as sinorhizobium. the judicial commission considered that this decision would not cause sufficient confusion to justify the conservation of the latter name. the subcommittee on the taxonomy of agrobacterium and rhizobium of the international committee on systematics of prokaryotes (icsp) publicly disagreed with this conclusion. was i ... | 2010 | 20453103 |
| cytogenetic analysis of two coprophanaeus species (scarabaeidae) revealing wide constitutive heterochromatin variability and the largest number of 45s rdna sites among coleoptera. | the coleopterans of scarabaeinae clade presents coprophanaeus (megaphanaeus) ensifer and c. (coprophanaeus) cyanescens (scarabaeidae) when they are studied cytogenetically by different techniques. the species present symmetric karyotypes, diploid number of 2n=20, and meta-submetacentric chromosomes. c. (m.) ensifer present an xy sex-determining mechanism and c. (c.) cyanescens an xy(p) parachute mechanism. analysis of constitutive heterochromatin (ch) in the two species revealed the presence of ... | 2010 | 20675144 |
| ensifer (sinorhizobium) sojae sp. nov., isolated from root nodules of glycine max grown in saline-alkaline soils in hebei province of china. | thirteen bacterial strains isolated from root nodules of soybean grown in saline-alkaline soils in the chinese province of hebei were identified as a unique group in genus ensifer (sinorhizobium) based upon box-pcr patterns, sequencing analyses of 16s rrna and housekeeping genes, and dna-dna hybridization. phenotypically, positive acid production and negative reduction in litmus milk and sensitivity to 50 µg ml(1) of amplicillin, as well as some other features could differentiate the novel group ... | 2010 | 20851917 |
| heterologous expression of anabaena sp. pcc7120 cyanophycin metabolism genes cpha1 and cphb1 in sinorhizobium (ensifer) meliloti 1021. | sinorhizobium meliloti infects leguminous plants resulting in a nitrogen-fixing symbiosis. free living cells accumulate poly(3-hydroxybutyrate) (phb) as carbon and energy source under imbalanced growth conditions. the cpha1 (7120) gene encoding a cyanophycin (cgp) synthetase of anabaena sp. pcc7120 in plasmids pvlt31::cpha1 (7120) and pbbr1mcs-3::cpha1 (7120) was expressed in the wild-type s. meliloti 1021 and in a phbc-negative mutant generated in this study. expression of cpha1 (7120) and accu ... | 2010 | 20938772 |
| effect of transgenic rhizobacteria overexpressing citrobacter braakii appa on phytate-p availability to mung bean plants. | rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-p available to plants. in the present study, citrobacter braakii phytase gene (appa) was over-expressed in rhizobacteria possessing plant growth promoting (pgp) traits for increasing their potential as bioinoculants. appa was cloned under the lac promoter in the broad host-range expression vector pbbr1mcs2. transformation of the recombinant construct pcbappa resulted in high constitutive phytase ... | 2010 | 21124052 |
| why two are not enough: degradation of p-toluenesulfonate by a bacterial community from a pristine site in moorea, french polynesia. | in previous work, only one culture (strain ta12) from a pristine site was reported to utilize the xenobiotic compound p-toluenesulfonate (tsa) as a sole source of carbon and energy for aerobic growth. 'strain ta12' has now been recognized as a community of three bacteria: achromobacter xylosoxidans ta12-a, ensifer adhaerens ta12-b and pseudomonas nitroreducens ta12-c. achromobacter xylosoxidans ta12-a and e. adhaerens ta12-b were identified as the tsa degraders. these two organisms contain sever ... | 2011 | 21204940 |
| complete genome sequence of the medicago microsymbiont ensifer (sinorhizobium) medicae strain wsm419. | ensifer (sinorhizobium) medicae is an effective nitrogen fixing microsymbiont of a diverse range of annual medicago (medic) species. strain wsm419 is an aerobic, motile, non-spore forming, gram-negative rod isolated from a m. murex root nodule collected in sardinia, italy in 1981. wsm419 was manufactured commercially in australia as an inoculant for annual medics during 1985 to 1993 due to its nitrogen fixation, saprophytic competence and acid tolerance properties. here we describe the basic fea ... | 2010 | 21304680 |
| first genomic analysis of the broad-host-range rhizobium sp. lpu83 strain, a member of the low-genetic diversity oregon-like rhizobium sp. group. | alfalfa (medicago sativa) is the most cultivated forage legume for cattle and animal feeding, occupying about 32 million hectares over the world. management of the n(2)-fixing symbiosis of this plant to maximize crop production is therefore an important objective. a fundamental constraint to this aim emerges when a moderately low soil ph hampers the establishment of an effective symbiosis with indigenous and/or inoculated rhizobia. besides the association of alfalfa with ensifer (sinorhizobium) ... | 2011 | 21329739 |
| characterization of root-nodulating bacteria associated to prosopis farcta growing in the arid regions of tunisia. | diversity of 50 bacterial isolates recovered from root nodules of prosopis farcta grown in different arid soils in tunisia, was investigated. characterization of isolates was assessed using a polyphasic approach including phenotypic characteristics, 16s rrna gene pcr--rflp and sequencing, noda gene sequencing and mlsa. it was found that most of isolates are tolerant to high temperature (40°c) and salinity (3%). genetic characterization emphasizes that isolates were assigned to the genus ensifer ... | 2011 | 21359955 |
| stress tolerance and symbiotic and phylogenic features of root nodule bacteria associated with medicago species in different bioclimatic regions of tunisia. | thirty two rhizobial isolates were obtained from different bioclimatic regions of tunisia using as trap plants, medicago sativa, medicago ciliaris, medicago polymorpha and medicago minima. to study their diversity and characterize them in relation to mediterranean conditions, abiotic stress resistance, symbiotic properties and genetic diversity in terms of 16s rrna and noda sequences were assessed. five isolates from m. sativa, three from m. ciliaris and three from m. minima could grow at 45°c. ... | 2011 | 21487201 |
| molecular diversity and phylogeny of rhizobia associated with lablab purpureus (linn.) grown in southern china. | as an introduced plant, lablab purpureus serves as a vegetable, herbal medicine, forage and green manure in china. in order to investigate the diversity of rhizobia associated with this plant, a total of 49 rhizobial strains isolated from ten provinces of southern china were analyzed in the present study with restriction fragment length polymorphism and/or sequence analyses of housekeeping genes (16s rrna, igs, atpd, glnii and reca) and symbiotic genes (nifh and nodc). the results defined the l. ... | 2011 | 21498018 |
| [isolation and degradation characteristics of acetochlor-degrading strain a-3]. | the strain a-3 which could use acetochlor as the sole nitrogen source was isolated from soils contaminated with acetochlor and the sludge of pesticide factory by enrichment culture. the strain was identified as ensifer adhaerens according to the results of morphology, physiology,and the phylogenetical analyses of 16s rrna sequence. it was observed that the degradation rate of acetochlor by strain a-3 was 33.6% after 10 days culture at the concentration of 10 mg/l. the composition of the culture ... | 2011 | 21528581 |
| soil factors exhibit greater influence than bacterial inoculation on alfalfa growth and nitrogen fixation. | in order to study effects of soil factors and bacterial inoculation on alfalfa (medicago sativa), plants were inoculated with ensifer meliloti l33 and azospirillum brasilense sp7 in pot experiments using two different soils separately as well as in a mixture. one soil was contaminated with chemical waste products; the other was an arable soil. soil factors, including availability of macro- and micronutrients as well as carbon and nitrogen contents were found to exhibit a much greater influence o ... | 2011 | 21627671 |
| maldi-tof mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family rhizobiaceae. | family rhizobiaceae includes fast growing bacteria currently arranged into three genera, rhizobium, ensifer and shinella, that contain pathogenic, symbiotic and saprophytic species. the identification of these species is not possible on the basis of physiological or biochemical traits and should be based on sequencing of several genes. therefore alternative methods are necessary for rapid and reliable identification of members from family rhizobiaceae. in this work we evaluated the suitability o ... | 2011 | 21655291 |
| [dehydrogenation by short ethoxy chain nonylphenol dehydrogenase from ensifer sp. as08]. | to study dehydrogenation by short ethoxy chain nonylphenols dehydrogenase (snpeo-dh) from ensifer sp. as08. | 2011 | 21800626 |
| phenotypic and genetic characterization of rhizobia associated with alfalfa in the hokkaido and ishigaki regions of japan. | twenty five rhizobial isolates were obtained from root nodules of medicago sativa inoculated with soil samples collected from the sapporo region and ishigaki island in japan. to study their diversity and characterize them in relation to the climatic conditions of their soils of origin, a polyphasic approach analyzing stress tolerance, symbiotic and genetic properties was used. stress tolerance assays revealed marked variations in salinity, ph and temperature tolerance. isolates originating from ... | 2011 | 21684705 |
| production of phytophthora infestans-resistant potato (solanum tuberosum) utilising ensifer adhaerens ov14. | based on the use of agrobacterium tumefaciens-mediated transformation commodity crop improvement through genetic engineering is the fastest adopted crop technology in the world (james 2010). however, the complexity of the agrobacterium patent landscape remains a challenge for non-patent holders who wish to generate novel varieties for a commercial purpose. the potential of non-agrobacterium strains (transbacter(™)) to modify a plant genome has previously been described. however, they are unlikel ... | 2011 | 21912851 |
| alliinase from ensifer adhaerens and its use for generation of fungicidal activity. | abstract: a bacterium ensifer adhaerens ferm p-19486 with the ability of alliinase production was isolated from a soil sample. the enzyme was purified for characterization of its general properties and evaluation of its application in on-site production of allicin-dependent fungicidal activity. the bacterial alliinase was purified 300-fold from a cell-free extract, giving rise to a homogenous protein band on polyacrylamide gel electrophoresis. the bacterial alliinase (96 kda) consisted of two id ... | 2011 | 21906328 |
| mutualism and adaptive divergence: co-invasion of a heterogeneous grassland by an exotic legume-rhizobium symbiosis. | species interactions play a critical role in biological invasions. for example, exotic plant and microbe mutualists can facilitate each other's spread as they co-invade novel ranges. environmental context may influence the effect of mutualisms on invasions in heterogeneous environments, however these effects are poorly understood. we examined the mutualism between the legume, medicago polymorpha, and the rhizobium, ensifer medicae, which have both invaded california grasslands. many of these inv ... | 2011 | 22174755 |
| characterization of rhizobia from legumes of agronomic interest grown in semi-arid areas of central spain relates genetic differences to soil properties. | a study of symbiotic bacteria from traditional agricultural legumes from central spain was performed to create a collection of rhizobia from soils differing in physicochemical, analytical and/or agroecological properties which could be well-adapted to the environmental conditions of this region, and be used for sustainable agricultural practices. thirty-six isolates were obtained from root-nodules of fifteen legume species (including cicer arietinum, lathyrus sativus, lens culinaris, lupinus spp ... | 2011 | 21953333 |
| mercury-resistant rhizobial bacteria isolated from nodules of leguminous plants growing in high hg-contaminated soils. | a survey of symbiotic bacteria from legumes grown in high mercury-contaminated soils (almadén, spain) was performed to produce a collection of rhizobia which could be well adapted to the environmental conditions of this region and be used for restoration practices. nineteen hg-tolerant rhizobia were isolated from nodules of 11 legume species (of the genera medicago, trifolium, vicia, lupinus, phaseolus, and retama) and characterized. based on their growth on hg-supplemented media, the isolates w ... | 2012 | 22246528 |