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parasite-related lesions in a bearded seal, erignathus barbatus.a free-ranging adult male bearded seal (erignathus barbatus) killed by polar bears was salvaged and examined at necropsy. significant findings included diffuse intrahepatic bile duct fibrosis and chronic cholangitis; multiple nodules of chronic fibrosing pancreatitis; and gastric ulcers. trematode eggs (family campulidae) were found in the pancreatic nodules. these eggs and the trematodes that produced them probably caused the pancreatic and liver lesions. phocanema decipiens and contracaecum os ...1979113564
helminths of the spotted seal, phoca largha, from the bering sea.fifty-five spotted seals, phoca largha, from the seasonal pack ice of the bering sea were examined for helminth parasites. twelve species of helminths were found: anophryocephalus ochotensis, diplogonoporus tetrapterus, diphyllobothrium cordatum, phocanema decipiens, contracaecum osculatum, dipetalonema spirocauda, phocitrema fusiforme, corynosoma semerme, c. strumosum, c. validum, c. villosum, and bolbosoma sp. six species are new host records (a. ochotensis, d. tetrapterus, d. cordatum, d. spi ...19827097871
[occurrence of parasites, bacteria, viruses and fungi in fish that are pathogenic to men and fish].parasitological examinations comprised above 20,000 fish which were searched for parasitic nematoda of anisakidae. it was evidenced that herring were infected with anisakid larvae in 8%, cod and flatfish in 4% and the eelpouts in as many as in 52%. the species that prevails in these fish were: anisakis simplex, contracaecum osculatum c, hysterothylacium auctum and pseudoterranova decipiens, respectively. in direction of the bacteria pathogenic to man 765 fish were examined; in 38% there were fou ...19957571631
in vitro development of the nematode contracaecum osculatum rudolphi 1802 (nematoda: anisakinae). 19744451901
[twin species in contracaecum osculatum (ascaridida, anisakidae): reproductive isolation and diagnostic features at the electrophoretic level]. 19863463233
contracaecum osculatum (nematoda:anisakidae) in fish and seals in bothnian bay (northeastern baltic sea). 19883397219
patterns of caudal papillae in contracaecum osculatum (nematoda) and some related species from different regions of the world. 19883220645
incubation in rats of a nematodal larva from cod to establish its specific identity: contracaecum osculatum, (rudolphi).infective, third-stage larvae of the genus contracaecum from fish were cultured in vitro and fed to laboratory animals in order to obtain later developmental stages to permit their specific identification. the in vitro incubation was unsuccessful. in orally infected laboratory rats and hamsters, fourth-stage larvae were obtained from the stomach at 2-5 days postinfection (p.i.), but no adult worms developed. larvae introduced surgically into the body cavity of laboratory rats yielded some adult ...19883205872
the helminth fauna from the common seal (phoca vitulina vitulina, linné, 1758) of the wadden sea in lower saxony. part 2: nematodes.during the seal epidemic in 1988 and the beginning of 1989, 115 common seals found dead on the shores of the wadden sea of lower saxony were investigated for the presence of nematodes. the lungworm otostrongylus circumlitus railliet 1899 was found in 26.1% of the seals, the lungworm parafilaroides gymnurus railliet 1899 in 26.9% and the heartworm dipetalonema spirocauda leidy 1858 in 32.2% of the seals. in the digestive tract, two anisakid species were found, pseudoterranova decipiens mozgovoi 1 ...19911789022
[finding of contracaecum osculatum (nematoda, ascaridata) larvae in fishes of the white sea]. 1979159732
comparison of a manual and an automated method to estimate the number of uterine eggs in anisakid nematodes: to coulter or not to coulter. is that the question?studies reporting numbers of eggs in vagina and utero in nematodes often give little information of the technique used for the estimations. this situation hampers comparison among studies, because, so far, differences in estimations provided by different techniques have not been assessed. this note examines whether a manual method based on visual counts in aliquots and an automated method using a coulter counter yield equivalent estimations of egg numbers in vagina and utero of 3 anisakid nemato ...200717539430
pathological findings in harbour porpoises (phocoena phocoena) from norwegian and icelandic waters.a study of 37 by-caught harbour porpoises from icelandic and norwegian waters showed that most were in good or moderate nutritional condition and none was severely emaciated. mild infection with lungworms (halocercus invaginatus, pseudalius inflexus, torynurus convolutus) was found in 84% of the icelandic and 91% of the norwegian animals, usually associated with bronchopneumonia which was rarely severe. most (91%) of the animals had parasites in the stomach and intestine (anisakis simplex, contr ...200616527299
prevalence of anisakin nematodes in fish from southern baltic sea.nematodes from the superfamily ascaridoidea (families anisakidae and raphidascarididae) are worldwide distributed parasites. their live cycles include many species of water invertebrates and teleostean fish as intermediate hosts, and fish, sea mammals or fish-eating birds being definitive hosts. humans can be infected with some of these parasites after consumption of raw or wrongly processed fish. the parasitological investigations of fish (herring, cod and flatfish) from southern baltic (ices 2 ...200516457379
identification of anisakid nematodes with zoonotic potential from europe and china by single-strand conformation polymorphism analysis of nuclear ribosomal dna.using genetic markers defined previously in the second internal transcribed spacer (its-2) of nuclear ribosomal dna (rdna), isotopic, and non-isotopic polymerase-chain-reaction-coupled single-strand conformation polymorphism (sscp) were utilized to identify each of three anisakid species [anisakis simplex (s.l.), contracaecum osculatum (s.l.), and hysterothylacium aduncum] from different host species and geographical locations in poland and sweden. while subtle microheterogeneity was observed wi ...200717694403
identification of anisakid nematodes from the southern baltic sea using pcr-based methods.four common species of baltic anisakids anisakis simplex, hysterothylacium auctum, contracaecum osculatum from fish (the last one also from the grey seal) and c. rudolphii from cormorants were examined using pcr-rflp technique for working out the method allowing their identification and differentiation. a fragment of nuclear dna containing intergenic regions (its-1 and its-2) of ribosomal dna together with adjacent sequences of genes coding 18s and 28s rrna (in total; approximately 1500 bp) was ...200212030761
an sem study of phocascaris cystophorae berland, 1964 (nematoda: anisakidae), a parasite of the hooded seal cystophora cristata.scanning electron microscopy was used to study the surface morphological characteristics (excretory pore, interlabia knobs, lips and adjacent structures, caudal papillae and papilla-like structures) of the nematode phocascaris cystophorae, a parasite from the stomach of the hooded seal cystophora cristata. a comparative morphological analysis was made between species of phocascaris and contracaecum osculatum (sensu lato), which are all parasitic in phocid seals.200211912341
mutation scanning for sequence variation in three mitochondrial dna regions for members of the contracaecum osculatum (nematoda: ascaridoidea) complex.anisakid nematodes of seals from different geographical origins, previously identified by multilocus enzyme electrophoresis as contracaecum osculatum a (coa), c. osculatum b (cob), c. osculatum c (coc), c. osculatum d (cod), c. osculatum e (coe) and c. osculatum baicalensis (cob), were characterised genetically using a mutation scanning approach, in order to define genetic markers for their specific identification and differentiation. three mitochondrial dna (mtdna) regions, namely cytochrome c ...200111358127
phylogenetic relationships among species of contracaecum railliet & henry, 1912 and phocascaris høst, 1932 (nematoda:ascaridoidea) based on nuclear rdna sequence data.nuclear-encoded large-subunit ribosomal dna sequences were used to infer a phylogenetic hypothesis for 17 taxa (16 nominal species) of the genera contracaecum and phocascaris. phylogenetic trees based on these data have been used to assess the validity of the taxonomic distinction between these genera, which was based on the presence or absence of certain structural features, rather than on explicit hypotheses of evolutionary history. phylogenetic hypotheses based on parsimony, likelihood, and n ...200011072909
assessing sequence variation in the internal transcribed spacers of ribosomal dna within and among members of the contracaecum osculatum complex (nematoda: ascaridoidea: anisakidae).the anisakid nematodes of seals from different geographical origins, previously identified as contracaecum osculatum a, c. osculatum b, c. osculatum c, c. osculatum d, c. osculatulm e and c. osculatum baicalensis by multilocus enzyme electrophoresis, were characterised using a dna approach. the first and second internal transcribed spacers (its-1, its-2) of ribosomal dna (rdna) were individually amplified by polymerase chain reaction (pcr) and analysed by single-strand conformation polymorphism ...200010952269
characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal dna sequences.larvae of three species of anisakid nematode from fish, anisakis simplex, hysterothylacium aduncum and contracaecum osculatum, were characterised genetically using a molecular approach. the nuclear ribosomal dna region spanning the first internal transcribed spacer, the 5.8s gene and the second internal transcribed spacer was amplified and sequenced. the lengths of the first and second internal transcribed spacer sequences of the three species ranged from 392 to 449 bp and 262 to 347 bp, respect ...19989925272
parasites and related pathological lesions in the gastrointestinal tract of a seal (phoka vitulina linnaeus).an immature seal, about one-year-old, died a natural death. at autopsy, a large number of contracaecum osculatum and pseudoterranova decipiens, as well as some of anisakis simplex were found in the stomach together with numerous corynosoma strumosum in the small intestine and colon. histopathological examination revealed epithelial desquamation, congestion and hemorrhage in the mucosa. there were cellular infiltration and fibroplasia around the nematodes, collapsed nematodes and molted nematode ...19989795905
fecundity of sealworm (pseudoterranova decipiens) infecting grey seals (halichoerus grypus) in the gulf of st. lawrence, canada: lack of density-dependent effects.uterine egg counts were determined by means of an electronic particle counter in individual female sealworm, pseudoterranova decipiens (nematoda: ascaridoidea: anisakidae) collected from grey seals (halichoerus grypus) from anticosti island, northern gulf of st. lawrence, in august and september 1992. mean number of eggs per female was 54,916.9 (+/- 51,866.4), with a maximum of 300,000. egg counts were strongly correlated with worm length, and less so with host sex and age, sealworm sex ratio, a ...19979421731
the life cycle of contracaecum osculatum (rudolphi, 1802) sensu stricto (nematoda, ascaridoidea, anisakidae) in view of experimental infections.hatched, ensheathed third-stage larvae of contracaecum osculatum, 300-320 microns long, were shown to be infective to copepods, to nauplius larvae of balanus and to small specimens of fishes (sticklebacks, o-group eelpout). other fishes such as gobies and small flatfishes became infected by ingesting infected crustaceans. cod were infected by being given infected small fishes. in the crustacean paratenic hosts, little growth of the larvae occurred, if any. in the liver sinusoids of sticklebacks ...19957567906
third-stage larvae emerge from eggs of contracaecum osculatum (nematoda, anisakidae).in contracaecum osculatum rudolphi, 1802 (nematoda, anisakidae) the first and the second ecdyses occur inside the egg. larvae forced out from the eggs by coverslip pressure are surrounded by 2 cuticles, revealing 2 previous ecdyses. it is thus the third-stage larva that emerges from the hatched egg and not the second-stage larva as previously believed. the cuticle of the first-stage larva is thin, smooth, and remains in the egg, whereas the cuticle of the second-stage larva is thick, striated, a ...19938410554
two new members in the contracaecum osculatum complex (nematoda, ascaridoidea) from the antarctic.the genetic structure of adults and larvae of contracaecum osculatum (sensu lato) from the antarctic is analyzed on the basis of 24 enzyme loci. significant deviations of genotype frequencies from the hardy-weinberg equilibrium were found, even in samples recovered from the same host. these data indicate that two distinct, reproductively isolated species coexist in c. osculatum (sensu lato) samples from the antarctic. they were provisionally designated c. osculatum d and e, as they do not corres ...19948070954
ascaridoidea: a simple dna assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds.eleven species belonging to superfamily ascaridoidea, which infect marine and freshwater fish, mammals, and fish-eating birds, were analyzed using a pcr-rflp method. the following species were investigated: anisakis pegreffi, a. physeteris, and a. simplex (parasites of fish and mammals), contracaecum osculatum, c. radiatum, and c. rudolphi (parasites of mammals and fish-eating birds), hysterothylacium aduncum (a parasite of fish), porrocaecum angusticolle, p. crassum, p. depressum, and p. ensica ...200212243736
[anisakidosis a marine parasitic zoonosis: unknown or emerging in peru?].the purpose of this review is to show the experimental studies carried out on the biological cycle, culture, pathogenicity of the anisakidae nematode larvae and to disseminate the information regarding current epidemy and the probable emergence of anisakidosis in peru, and in addition, to propose measures of prevention and control, as well as the perspective and need for investigation. the studies of experimental pathogenicity in cats, dos, and hamsters are incomplete. eight cases of acute human ...200415614302
[helminth fauna of bufo raddei in transbaikalia].helminth fauna of bufo raddei from transbaikalia has been studied in 2003-2007. eleven species of parasites nematoda were found in this host, namely rhabdias bufonis (schrank, 1788), oswaldocruzia filiformis (goeze, 1782), oswaldocruzia yezoensis morishita, 1926, contracaecum osculatum (rudolphi, 1802), raphidascaris acus, larvae, aplectana acuminata (schrank, 1788), aplectana multipapillosa ivanitzky, 1940, cosmocerca commutata (diesing, 1851), cosmocerca ornata (dujardin, 1845), cosmocercoids ...201020536007
three sibling species within contracaecum osculatum (nematoda, ascaridida, ascaridoidea) from the atlantic arctic-boreal region: reproductive isolation and host preferences.genetic variation within and between population samples from 22 locations of the atlantic arctic-boreal region, including 1657 specimens morphologically assigned to contracaecum osculatum, was electrophoretically analysed at 17 loci. highly significant deviations from the hardy-weinberg equilibrium were found at various loci in several samples, owing to the existence of three distinct gene pools within c. osculatum (sensu lato) from the study area. these gene pools correspond to three biological ...19938468125
parasitic infection of an endemic fish (blicca bjoerkna) and an exotic fish (hemiculter beucisculus) in anzali lagoon, caspian sea, iran.in anzali lagoon, there are some endemic and exotic fishes. the present study was conducted to compare the parasitic fauna of blicca bjeorkna, as an endemic fish and hemiculter leucisculus, as an introduced fish to the lagoon.201122347299
occurrence of zoonotic nematodes pseudoterranova decipiens, contracaecum osculatum and anisakis simplex in cod (gadus morhua) from the baltic sea.baltic cod gadus morhua (a total of total 224 specimens) captured east of the island of bornholm in the southern baltic sea were subjected to a parasitological investigation between march 2013 and april 2014. full artificial digestion of fillets from 188 cod and additional investigation of livers from 36 cod were performed. cod or seal worm pseudoterranova decipiens was recorded in musculature (prevalences up to 55% and intensities up to 56 worms per fish) associated with a negative correlation ...201425224792
anisakids (nematoda: anisakidae) from stomachs of northern fur seals (callorhinus ursinus) on st. paul island, alaska: parasitological and pathological analysis.anisakid nematodes parasitize the alimentary tracts of aquatic vertebrates, including northern fur seals (callorhinus ursinus) (nfs). the main purpose of this study was to estimate the prevalence, intensity, and species composition of anisakids in nfss on st. paul island, alaska, and to analyze changes in nfs infection with these nematodes during the last decades. the study was carried out on st. paul island, alaska, in july-august 2011-2013. stomachs of 443 humanely harvested nfs males were col ...201425216781
anisakids of seals found on the southern coast of baltic sea.in the present study 5 grey seals (halichoerus grypus), 3 common seals (phoca vitulina) and 1 ringed seal (pusa hispida) bycaught or stranded on the polish baltic sea coast in years 2000-2006 were investigated for the infestation of parasitic anisakid nematodes. 749 of anisakids were found. the most common were: contracaecum osculatum (59.3%) and pseudoterranova decipiens (31.0%). there were also small numbers of anisakis simplex (0.8%). after performing rflp three sibling species were found. c. ...201424570064
mitochondrial genomes of anisakis simplex and contracaecum osculatum (sensu stricto)--comparisons with selected nematodes.anisakid nematodes parasitize mainly fish, marine mammals and/or fish-eating birds, and can be transmitted to a range of fish-eating mammals, including humans, where they can cause gastrointestinal disease linked to larval infection or allergic responses. in spite of the animal and human health significance of these parasites, there are still gaps in our understanding of the systematics, biology, epidemiology and ecology of anisakids. mitochondrial (mt) dna provides useful genetic markers for in ...201424211683
comparative infectivity of three larval nematode species in three different salmonids.host specificity of parasites may depend both on ecological and physiological factors. basic descriptions of the susceptibility/resistance of fish to specific nematodes are needed in order to reveal mechanisms in the host-parasite relation. rainbow trout (oncorhynchus mykiss), brown trout (salmo trutta), and atlantic salmon (salmo salar) were orally infected with larval stages of three different anisakid nematodes-hysterothylacium aduncum, contracaecum osculatum, and anisakis simplex-and parasit ...201323733234
anisakid nematodes of greenland halibut reinhardtius hippoglossoides from the barents sea.parasitic nematodes from the body cavity of 94 greenland halibut (reinhardtius hippoglossoides) caught in the barents sea were examined. nematode species were identified using both morphological and molecular methods. four morphospecies were found: anisakis simplex s.l., contracaecum osculatum s.l., pseudoterranova decipiens s.l., and hysterothylacium aduncum. the most prevalent were c. osculatum s.l. (89.4%) and a. simplex s.l. (86.2%). mean intensities for these 2 species were 7.8 and 45.2, re ...201323536987
[infestation rates of the main commercial fish species with larvae of contracaecum osculatum (rudolphi, 1802) (nematoda: anisakidae) in russian waters of the south baltic in 2000-2012].the infestation dynamic of the sprat (sprattus sprattus balticus), the baltic herring (clupea harengus membras), and the baltic cod (gadus morhua callaris) with nematodes contracaecum osculatum 1 (anisakidae) from russian waters of the south baltic were studied in 2000-2012. peculiarities of larval growth in different hosts were analyzed. a total of 6233 fish specimens were investigated, including 1420, 3867, and 946 specimens of sprat, herring, and cod, respectively. the prevalence and mean int ...201525693327
third-stage nematode larvae of contracaecum osculatum from baltic cod (gadus morhua) elicit eosinophilic granulomatous reactions when penetrating the stomach mucosa of pigs.third-stage larvae of the anisakid nematode contracaecum osculatum were recovered from livers of atlantic cod (gadus morhua) caught in the baltic sea (june 2014) and used for experimental infection of two pigs (one male and one female). each pig received 215 larvae by oral infection (feeding with minced cod liver containing live nematode larvae). pigs were euthanized after 5 days, necropsied, and subjected to parasitological investigation. a total of 12 larvae were found penetrating the mucosa o ...201525563618
sealworm (pseudoterranova decipiens) infection in grey seals (halichoerus grypus), cod (gadus morhua) and shorthorn sculpin (myoxocephalus scorpius) in the baltic sea.the anisakid nematode pseudoterranova decipiens, known as the sealworm or cod worm, can infect the flesh of several fish species. the parasite causes cosmetic problems for the fish industry and can cause abdominal discomfort if consumed by humans. there are only scattered studies on the abundance or distribution of the sealworm in fish and seals in the baltic sea. to remedy this situation, the extent of sealworm infection was investigated in cod (gadus morhua) and shorthorn sculpin (myoxocephalu ...201525367209
increased contracaecum osculatum infection in baltic cod (gadus morhua) livers (1982-2012) associated with increasing grey seal (halichoerus gryphus) populations.grey seals (halichoerus gryphus), the main final host of the gastric parasitic nematode contracaecum osculatum in the baltic, have recently recolonized the southwestern baltic sea. this colonization could lead to an increase in prevalence and intensity of third-stage larvae of c. osculatum in livers of baltic cod (gadus morhua), which serve as transport host for this helminth. we performed a parasitologic study of cod in spring 2012 and compared the results with previously unpublished data from ...201424779467
parasite communities of icefish (chionodraco hamatus) in the ross sea (antarctica): influence of the host sex on the helminth infracommunity structure.parasite communities of chionodraco hamatus were investigated from terra nova bay (ross sea, antarctica) during host spawning time. special attention was given to helminth infracommunities and effect of host sex on its structure. a total of 21 taxa including 5 ecto-parasites and 16 endo-parasites were identified. the number of ecto and endo-parasite species per individual host ranged from 1 to 3 and 3 to 10, respectively, while the mean numbers of parasite specimens per individual host were 4.7 ...201424558440
parasitic infection by larval helminths in antarctic fishes: pathological changes and impact on the host body condition index.we examined pathological changes and relationship between body condition index (bci) and parasitic infection in 5 species of fish, including 42 icefish chionodraco hamatus (channichtyidae), 2 dragonfish cygnodraco mawsoni (bathydraconidae), 30 emerald rock cod trematomus bernacchii, 46 striped rock cod t. hansoni and 9 dusty rock cod t. newnesi (nototheniidae) from the ross sea, antarctica. all parasites were identified by a combination of morphology and mtdna cytochrome-oxidase-2 sequence (mtdn ...201323872857
pathological changes of stomach in ringed seal (pusa hispida) from arviat (north canada) caused by anisakid nematodes.this is a report of lesions associated with the nematodes (anisakidae) from the stomach of ringed seals (pusa hispida). on thirty one examined ringed seals from arviat, thirteen were infected by 1 to 24 anisakids. identification of nematodes from their stomachs showed two species; contracaecum osculatum (sensu lato) (79 specimens) and pseudoterranova decipiens (sensu lato) (11 specimens). in the gastric sections with parasites, larvae and adults of nematodes were present mainly in the fundic por ...201323691577
multiplex pcr for the identification of anisakis simplex sensu stricto, anisakis pegreffii and the other anisakid nematodes.a multiplex pcr method was established for the rapid identification of anisakis simplex sensu stricto, a. pegreffii, a. physeteris, pseudoterranova decipiens, contracaecum osculatum and hysterothylacium aduncum. the sequence alignment of the internal transcribed spacer 1 region (its-1) between a. simplex s. str. and a. pegreffii showed a high degree of similarity, but only two c-t transitions were observed. to differentiate a. simplex s. str. from a. pegreffii, an intentional mismatch primer wit ...200817962067
application of the pcr-sequence-specific primers for the discrimination among larval anisakis simplex complex.the fish-borne zoonotic nematode, anisakis simplex, is not a single specie but a complex composed of three sibling species, a. simplex sensu stricto, anisakis pegreffii, and a. simplex c. discrimination among these sibling species have been performed by polymerase chain reaction (pcr)-restriction fragment length polymorphism or sequencing analysis of the internal transcribed spacer (its) region of ribosomal dna (rdna). in the present study, pcr-sequence-specific primers were applied for the disc ...200818239942
great sandeel (hyperoplus lanceolatus) as a putative transmitter of parasite contracaecum osculatum (nematoda: anisakidae).in the baltic sea, the great sandeel (hyperoplus lanceolatus), a common fish species that inhabits sandy substrates, is an important element in the diet of marine mammals, sea birds, and piscivorous fish. it can also act as a transmitter of parasites to its predators, but parasitological studies on this species are limited. we sampled great sandeels in april 2016 in two areas of the southern baltic sea (sb-south of bornholm and gg-the gulf of gdansk) and found parasitic anisakid nematodes in bot ...201728516228
contracaecum osculatum and other anisakid nematodes in grey seals and cod in the baltic sea: molecular and ecological links.populations of grey seals (halichoerus grypus), sprats (sprattus sprattus) and cod (gadus morhua) in the baltic sea are relatively stationary. the present work, applying classical and molecular helminthological techniques, documents that seals and cod also share a common parasite, the anisakid nematode contracaecum osculatum, which uses seals as the final host and fish as transport hosts. sequencing mitochondrial genes (cox1 and cox2) in adult worms from seals and third-stage larvae from livers ...201728124629
species composition and infection dynamics of ascaridoid nematodes in barents sea capelin (mallotus villosus) reflecting trophic position of fish host.capelin (mallotus villosus) is among the most abundant fish species in the barents sea, and represents a critical food source for many predators in the area including atlantic cod and harp seal. in norway, the fish is of economic importance since whole capelin and roe are valuable export products. despite its economic and ecological importance, the parasites of barents sea capelin are poorly known. however, the presence of parasites in the edible parts may adversely affect product quality and co ...201627473836
anisakidae in beluga whales delphinapterus leucas from hudson bay and hudson strait.a total of 190 nematodes was isolated from the stomachs of 13 beluga whales delphinapterus leucas from the arctic part of hudson bay and hudson strait. infection intensity ranged from 1 to 57 specimens and prevalence was 84.62%. morphological examination of the nematodes revealed the presence of 3 species: pseudoterranova decipiens sensu lato, contracaecum osculatum s.l., and anisakis simplex s.l. molecular analysis by pcr-based restriction fragment length polymorphism (pcr-rflp) resulted in the ...201526119295
host size-dependent anisakid infection in baltic cod gadus morhua associated with differential food preferences.a significant increase in the infection level of baltic cod gadus morhua with the anisakid nematode larvae contracaecum osculatum and pseudoterranova decipiens has been recorded during recent years due to the expanding local population of grey seals halichoerus grypus, which act as final hosts for these parasites. here, we report from an investigation of 368 cod (total length [tl] 6-49 cm; caught in ices subdivision 25) that the infection level of juvenile cod (tl 6-30 cm) with larvae of c. oscu ...201627304871
temporal stability of parasite distribution and genetic variability values of contracaecum osculatum sp. d and c. osculatum sp. e (nematoda: anisakidae) from fish of the ross sea (antarctica).the ross sea, eastern antarctica, is considered a "pristine ecosystem" and a biodiversity "hotspot" scarcely impacted by humans. the sibling species contracaecum osculatum sp. d and c. osculatum sp. e are anisakid parasites embedded in the natural antarctic marine ecosystem. aims of this study were to: identify the larvae of c. osculatum (s.l.) recovered in fish hosts during the xxvii italian expedition to antarctica (2011-2012); perform a comparative analysis of the contemporary parasitic load ...201526767164
molecular studies on larvae of pseudoterranova parasite of trichiurus lepturus linnaeus, 1758 and pomatomus saltatrix (linnaeus, 1766) off brazilian waters.pseudoterranova larvae parasitizing cutlassfish trichiurus lepturus and bluefish pomatomus saltatrix from southwest atlantic coast of brazil were studied in this work by morphological, ultrastructural and molecular approaches. the genetic analysis were performed for the its2 intergenic region specific for pseudoterranova decipiens, the partial 28s (lsu) of ribosomal dna and the mtdna cox-1 region. we obtained results for the 28s region and mtdna cox-1 that was amplified using the polymerase chai ...201526408586
selected mitochondrial genes as species markers of the arctic contracaecum osculatum complex.this study, aimed at testing the hypothesis that some mitochondrial genes can serve as species-specific markers, involved a comparison of the sequence variance of selected mitochondrial dna genes of the arctic contracaecum osculatum species (c. osculatum a, c. osculatum b and c. osculatum c). we compared differences between five complete (nd2, cytb, nd3, nd4l and nd6) and three partial (co1, co3 and nd5) protein-coding genes. the total length of the sequence of each of the 13 specimens was 4830  ...201121749741
extrusion of contracaecum osculatum nematode larvae from the liver of cod (gadus morhua).baltic cod livers have during recent years been found increasingly and heavily infected with third-stage larvae of contracaecum osculatum. the infections are associated with an increasing population of grey seals which are final hosts for the parasite. heavy worm burdens challenge utilization and safety of the fish liver products, and technological solutions for removal of worms are highly needed. we investigated the attachment of the worm larvae in liver tissue by use of histochemical technique ...201728795224
immunomodulatory effects of excretory/secretory compounds from contracaecum osculatum larvae in a zebrafish inflammation model.excretory/secretory (es) compounds isolated from third-stage larvae of the anisakid nematode contracaecum osculatum parasitizing liver of baltic cod were investigated for effects on immune gene expression in a zebrafish lps-induced inflammation model. es products containing a series of proteins, of which some had enzymatic activity, were injected solely or with lps. es proteins alone induced up-regulation of a number of immune-related genes, but generally to a lower degree compared to lps. when ...201728742146
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