| anaerostipes caccae gen. nov., sp. nov., a new saccharolytic, acetate-utilising, butyrate-producing bacterium from human faeces. | two strains of a previously undescribed eubacterium-like bacterium were isolated from human faeces. the strains are gram-variable, obligately anaerobic, catalase negative, asporogenous rod-shaped cells which produced acetate, butyrate and lactate as the end products of glucose metabolism. the two isolates displayed 99.9% 16s rrna gene sequence similarity to each other and treeing analysis demonstrated the faecal isolates are far removed from eubacterium sensu stricto and that they represent a ne ... | 2002 | 12086188 |
| lactate-utilizing bacteria, isolated from human feces, that produce butyrate as a major fermentation product. | the microbial community of the human colon contains many bacteria that produce lactic acid, but lactate is normally detected only at low concentrations (<5 mm) in feces from healthy individuals. it is not clear, however, which bacteria are mainly responsible for lactate utilization in the human colon. here, bacteria able to utilize lactate and produce butyrate were identified among isolates obtained from 10(-8) dilutions of fecal samples from five different subjects. out of nine such strains ide ... | 2004 | 15466518 |
| two routes of metabolic cross-feeding between bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut. | dietary carbohydrates have the potential to influence diverse functional groups of bacteria within the human large intestine. of 12 bifidobacterium strains of human gut origin from seven species tested, four grew in pure culture on starch and nine on fructo-oligosaccharides. the potential for metabolic cross-feeding between bifidobacterium adolescentis and lactate-utilizing, butyrate-producing firmicute bacteria related to eubacterium hallii and anaerostipes caccae was investigated in vitro. e. ... | 2006 | 16672507 |
| cross-feeding between bifidobacterium longum bb536 and acetate-converting, butyrate-producing colon bacteria during growth on oligofructose. | in vitro coculture fermentations of bifidobacterium longum bb536 and two acetate-converting, butyrate-producing colon bacteria, anaerostipes caccae dsm 14662 and roseburia intestinalis dsm 14610, with oligofructose as the sole energy source, were performed to study interspecies interactions. two clearly distinct types of cross-feeding were identified. a. caccae dsm 14662 was not able to degrade oligofructose but could grow on the fructose released by b. longum bb536 during oligofructose breakdow ... | 2006 | 17056678 |
| isolation of lactate-utilizing butyrate-producing bacteria from human feces and in vivo administration of anaerostipes caccae strain l2 and galacto-oligosaccharides in a rat model. | lactate-utilizing butyrate-producers were isolated from human feces and identified based on the sequences of 16s rrna gene. anaerostipes caccae strain l2, one of the seven human fecal isolates, was administered to rats with galacto-oligosaccharides (gos) as bifidogenic carbohydrates for stimulating lactate formation in the hindgut. ingestion of gos alone increased concentrations of cecal lactate and butyrate compared with control rats (p<0.05). additional administration of strain l2 on gos tende ... | 2008 | 18554304 |
| in vitro kinetics of prebiotic inulin-type fructan fermentation by butyrate-producing colon bacteria: implementation of online gas chromatography for quantitative analysis of carbon dioxide and hydrogen gas production. | kinetic analyses of bacterial growth, carbohydrate consumption, and metabolite production of five butyrate-producing clostridial cluster xiva colon bacteria grown on acetate plus fructose, oligofructose, inulin, or lactate were performed. a gas chromatography method was set up to assess h2 and co2 production online and to ensure complete coverage of all metabolites produced. method accuracy was confirmed through the calculation of electron and carbon recoveries. fermentations with anaerostipes c ... | 2009 | 19633122 |
| anaerostipes butyraticus sp. nov., an anaerobic, butyrate-producing bacterium from clostridium cluster xiva isolated from broiler chicken caecal content, and emended description of the genus anaerostipes. | four butyrate-producing isolates were obtained from the caecal content of a 4-week-old broiler chicken. the 16s rrna gene sequences were determined and confirmed the close relatedness of the four isolates, which suggested that they were derived from a single bacterial clone. phylogenetic analysis based on 16s rrna gene sequences showed that its closest relatives were members of cluster xiva of the clostridium subphylum of gram-positive bacteria and that the closest related type strain was anaero ... | 2010 | 19666785 |
| lactate has the potential to promote hydrogen sulphide formation in the human colon. | high concentrations of sulphide are toxic for the gut epithelium and may contribute to bowel disease. lactate is a favoured cosubstrate for the sulphate-reducing colonic bacterium desulfovibrio piger, as shown here by the stimulation of sulphide formation by d. piger dsm749 by lactate in the presence of sulphate. sulphide formation by d. piger was also stimulated in cocultures with the lactate-producing bacterium bifidobacterium adolescentis l2-32. other lactate-utilizing bacteria such as the bu ... | 2009 | 19732152 |
| na(+)-translocating membrane pyrophosphatases are widespread in the microbial world and evolutionarily precede h(+)-translocating pyrophosphatases. | membrane pyrophosphatases (ppases), divided into k(+)-dependent and k(+)-independent subfamilies, were believed to pump h(+) across cell membranes until a recent demonstration that some k(+)-dependent ppases function as na(+) pumps. here, we have expressed seven evolutionarily important putative ppases in escherichia coli and estimated their hydrolytic, na(+) transport and h(+) transport activities as well as their k(+) and na(+) requirements in inner membrane vesicles. four of these enzymes (fr ... | 2011 | 21527638 |
| human intestinal microbiota: characterization of a simplified and stable gnotobiotic rat model. | the study of host microbe interactions is hampered by the complexity and inter-individual variability of the human gut microbiota. therefore, a simplified human intestinal microbiota (sihumi) consisting of seven bacterial species was introduced into germfree rats. species selection was based on numerical importance and fermentative abilities in the human gut. association of the rats with the sihumi (anaerostipes caccae, bacteroides thetaiotaomicron, bifidobacterium longum, blautia producta, clos ... | 2010 | 21637015 |
| arabinoxylans and inulin differentially modulate the mucosal and luminal gut microbiota and mucin-degradation in humanized rats. | the endogenous gut microbiota affects the host in many ways. prebiotics should favour beneficial intestinal microbes and thus improve host health. in this study, we investigated how a novel class of potential prebiotic long-chain arabinoxylans (lc-ax) and the well-established prebiotic inulin (in) modulate the gut microbiota of humanized rats. six weeks after axenic rats were inoculated with a human faecal microbiota, their colonic microbiota was similar to this inoculum (∼ 70%), whereas their c ... | 2011 | 21883787 |
| lactate- and acetate-based cross-feeding interactions between selected strains of lactobacilli, bifidobacteria and colon bacteria in the presence of inulin-type fructans. | cross-feeding interactions were studied between selected strains of lactobacilli and/or bifidobacteria and butyrate-producing colon bacteria that consume lactate but are not able to degrade inulin-type fructans (itf) in a medium for colon bacteria (supplemented with itf as energy source and acetate when necessary). degradation of oligofructose by lactobacillus acidophilus ibb 801 and inulin by lactobacillus paracasei 8700:2 and bifidobacterium longum lmg 11047 resulted in the release of free fru ... | 2017 | 27810444 |
| bypass graft infection and bacteremia caused by anaerostipes caccae: first report of human infection caused by a recently described gut anaerobe. | we report a case of bypass graft infection and bacteremia caused by anaerostipes caccae. a review of the literature shows no reported human infection caused by this microorganism to date. the patient was initially treated with vancomycin and piperacillin-tazobactam on admission and with amoxicillin-clavulanate upon discharge. the slow-growing organism was subsequently found to be susceptible to metronidazole and ertapenem. | 2016 | 27693544 |
| ethanol production by selected intestinal microorganisms and lactic acid bacteria growing under different nutritional conditions. | to gain some specific insight into the roles microorganisms might play in non-alcoholic fatty liver disease (nafld), some intestinal and lactic acid bacteria and one yeast (anaerostipes caccae, bacteroides thetaiotaomicron, bifidobacterium longum, enterococcus fecalis, escherichia coli, lactobacillus acidophilus, lactobacillus fermentum, lactobacillus plantarum, weissella confusa, saccharomyces cerevisiae) were characterized by high performance liquid chromatography for production of ethanol whe ... | 2016 | 26858714 |
| association of germ-free mice with a simplified human intestinal microbiota results in a shortened intestine. | genetic, nutritional, and gut microbiota-derived factors have been proposed to play a role in the development of the whole intestine that is around 40% longer in prm/alf mice compared with other mouse strains. the prm/alf genotype explains 60% of this length difference. the remaining 40% are due to a maternal effect that could depend on the gut microbiota transmitted by the mother to their pups. germ-free prm/alf mice and c3h/he mice were associated with a simplified human microbiota (sihumi) to ... | 2015 | 24637599 |
| anaerostipes rhamnosivorans sp. nov., a human intestinal, butyrate-forming bacterium. | a novel butyrate-producing bacterium, strain 1y-2(t), was isolated from a stool sample of a 1-year-old, healthy dutch infant. the isolate was obtained by using lactate and acetate as sources of carbon and energy. the strain was gram-variable, strictly anaerobic and spore-forming and formed curly rod-shaped cells that fermented glucose into butyrate, lactate, formate and acetate as main products. the dna g+c content of the strain was 44.5 mol% and its major cellular fatty acids were c12:0, iso-c1 ... | 2014 | 24215821 |
| anaerostipes hadrus comb. nov., a dominant species within the human colonic microbiota; reclassification of eubacterium hadrum moore et al. 1976. | recent molecular analyses suggest that bacteria related to strains ss2/1 and ssc/2, previously reported to be distantly related to anaerostipes caccae ncimb 13811(t), represent one of the ten most abundant phylotypes detected in adult human faecal samples. these two strains were isolated as d-lactate-utilizing bacteria from faecal samples of a healthy individual. we show here that they share >99.9% similarity in 16s rrna gene sequence with a new butyrate-producing isolate recovered from a coloni ... | 2012 | 22982042 |