| aeromonas popoffii sp. nov., a mesophilic bacterium isolated from drinking water production plants and reservoirs. | we examined the taxonomic position of seven aeromonas isolates, recovered from flemish and scottish drinking water production plants and reservoirs, which were previously recognized by numerical analysis of genomic aflp fingerprints as members of an unknown aeromonas taxon that most closely resembled the species aeromonas bestiarum (dna hybridization group [hg] 2). the new phenotypic and dna-dna hybridization data obtained in this study show that the a. bestiarum-like strains constitute a separa ... | 1997 | 9336924 |
| epidemiological relationships between aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping. | ribotyping was used to study the epidemiology of aeromonas associated gastro-enteritis in young children. ribotyping patterns of 29 aeromonas strains (16 aeromonas caviae, 8 aeromonas hydrophila, 3 aeromonas eucrenophila, 1 aeromonas veronii, and 1 aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the gelcompare software with patterns of 104 strains (39 aeromonas eucrenophila, 29 aeromonas caviae, 11 aeromonas encheleia, 10 aeromonas hydrophila, 6 aer ... | 2000 | 10997832 |
| extended method for discrimination of aeromonas spp. by 16s rdna rflp analysis. | a previously described molecular method, based on 16s rdna rflp analysis, for the identification of aeromonas spp. was unable to separate the species aeromonas salmonicida, aeromonas bestiarum and the recently described aeromonas popoffii. in this study, the method has been extended with endonucleases aiwni and psti for the identification of these species. a molecular frame for the identification of all known aeromonas spp. is presented. | 2000 | 11155981 |
| mesophilic aeromonads in wild and aquacultured freshwater fish. | numbers and species of motile aeromonas were determined in freshly caught freshwater fish, in the surrounding environment, and also during iced chilled storage of fish specimens. although no significant differences were observed in water samples, initial levels for skin, gill, and intestines were significantly lower in farmed rainbow trout (oncorhynchus mykiss) than in wild brown trout (salmo trutta) and pike (esox lucius). during storage of wild specimens, naturally occurring aeromonads grew fa ... | 2001 | 11348001 |
| phenotypic characteristics and pathogenicity of aeromonas genomospecies isolated from common carp (cyprinus carpio l.). | to evaluate the relationship between the genomospecies, phenotypic profile and pathogenicity for carp of 37 motile aeromonas strains. | 2002 | 12452960 |
| characterisation of aeromonas spp. isolated from frozen fish intended for human consumption in mexico. | a total of 82 strains of presumptive aeromonas spp. were identified biochemically and genetically (16s rdna-rflp). the strains were isolated from 250 samples of frozen fish (tilapia, oreochromis niloticus niloticus) purchased in local markets in mexico city. in the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may play an important role in bacterial infection. in addition, we studied the antimicrobial patterns of t ... | 2003 | 12781953 |
| influence of temperature on the immune response of carp to lipopolysaccharide and whole cell aeromonas bestiarum vaccines. | immunisation of carp, cyprinus carpio, with four different experimental vaccines for protection against a. bestiarum was tested. carp immunized with different antigens of a. bestiarum p1s, and kept at 18 degrees c responded immunologically by relatively high titers of antibodies. antibody production was inhibited at 12 degrees c. | 2003 | 14509353 |
| the effect of various aeromonas bestiarum vaccines on non-specific immune parameters and protection of carp (cyprinus carpio l.). | aeromonas bestiarum is one of the causal agents of motile aeromonad infection/motile aeromonad septicemia (mai/mas) in fish. infections of the bacterium is an increasing problem in commercial carp (cyprinus carpio l.) farmed in poland. non-specific immune response of the fish, vaccinated with oil-emulsified experimental vaccines containing formalin killed whole cells (wcs), formalin killed whole culture (wct) or crude lps (50 or 1250 microg per fish) were studied on days 7 and 30 after vaccinati ... | 2004 | 15123310 |
| phylogenetic analysis of the genus aeromonas based on two housekeeping genes. | the phylogenetic relationships of all known species of the genus aeromonas, and especially aeromonas bestiarum and aeromonas salmonicida, were investigated on 70 strains using the rpod sequence, which encodes the sigma70 factor. this analysis was complemented with the sequence of gyrb, which has already proven useful for determining the phylogenetic relationships in the genus. nucleotide sequences of rpod and gyrb showed that both genes had similar substitution rates (< 2 %) and a similar number ... | 2004 | 15388703 |
| phenotypic, genotypic, and phylogenetic discrepancies to differentiate aeromonas salmonicida from aeromonas bestiarum. | the taxonomy of the "aeromonas hydrophila" complex (comprising the species a. hydrophila, a. bestiarum, a. salmonicida, and a. popoffii) has been controversial, particularly the relationship between the two relevant fish pathogens a. salmonicida and a. bestiarum. in fact, none of the biochemical tests evaluated in the present study were able to separate these two species. one hundred and sixteen strains belonging to the four species of this complex were identified by 16s rdna restriction fragmen ... | 2005 | 16562378 |
| complete sequence of the flor-carrying multiresistance plasmid pab5s9 from freshwater aeromonas bestiarum. | a multiresistant aeromonas bestiarum strain, shown to be persistent and spreading in a freshwater stream, was investigated for the presence, location and organization of antimicrobial resistance genes. | 2008 | 18413319 |
| efficacy of in-feed probiotics against aeromonas bestiarum and ichthyophthirius multifiliis skin infections in rainbow trout (oncorhynchus mykiss, walbaum). | the aim of this study was to assess the efficacy of in-feed probiotics as a preventive measure against skin infections caused by aeromonas bestiarum and ichthyophthirius multifiliis (ich) in rainbow trout. | 2008 | 18422955 |
| characterization of aeromonas encheleia strains isolated from aquatic environments in the czech republic. | characterization and identification of aeromonas strains isolated from surface and underground waters using phenotypic and genotyping methods. | 2009 | 19055626 |
| aeromonas piscicola sp. nov., isolated from diseased fish. | four aeromonas strains (s1.2(t), eo-0505, tc1 and ti 1.1) isolated from moribund fish in spain showed a restriction fragment length polymorphism (rflp) pattern related to strains of aeromonas salmonicida and aeromonas bestiarum but their specific taxonomic position was unclear. multilocus sequence analysis (mlsa) of housekeeping genes rpod, gyrb, reca and dnaj confirmed the allocation of these isolates to an unknown genetic lineage within the genus aeromonas with a. salmonicida, a. bestiarum and ... | 2009 | 19570633 |
| identification and pathogenicity to rainbow trout, oncorhynchus mykiss (walbaum), of some aeromonads. | twelve strains of fish pathogenic aeromonads were identified by 16s rrna sequencing as aeromonas bestiarum, a. hydrophila, a. hydrophila subsp. dhakensis, a. salmonicida subsp. salmonicida, a. sobria biovar sobria and a. veronii biovar sobria. following intramuscular injection, a. hydrophila subsp. dhakensis caused dark liquefying, raised furuncle-like lesions in rainbow trout within 48 h. extracellular products of all cultures contained gelatinase and lecithinase, and most revealed lipase. cong ... | 2009 | 19602093 |
| the structure of the o-specific polysaccharide from the lipopolysaccharide of aeromonas bestiarum strain 207. | the o-specific polysaccharide obtained by mild-acid degradation of aeromonas bestiarum 207 lipopolysaccharide was studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy. the sequence of the sugar residues was determined by roesy and hmbc experiments. it is concluded that the o-polysaccharide is composed of branched pentasaccharide repeating units of the following structure: [structure: see the text] | 2010 | 20080230 |
| structural characterization of the o-specific polysaccharide from the lipopolysaccharide of the fish pathogen aeromonas bestiarum strain p1s. | the o-specific polysaccharide obtained by mild-acid degradation of lipopolysaccharide of aeromonas bestiarum p1s was studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy. the sequence of the sugar residues was determined using (1)h,(1)h noesy and (1)h,(13)c hmbc experiments. the o-specific polysaccharide was found to be a high-molecular-mass polysaccharide composed of tetrasaccharide repeating units of the structure [formula in text]. since small amounts of a term ... | 2011 | 21377659 |
| structural and immunochemical studies of the lipopolysaccharide from the fish pathogen, aeromonas bestiarum strain k296, serotype o18. | chemical analyses and mass spectrometry were used to study the structure of the lipopolysaccharide (lps) isolated from aeromonas bestiarum strain k296, serotype o18. esi-ms revealed that the most abundant a. bestiarum lps glycoforms have a hexa-acylated or tetra-acylated lipid a with conserved architecture of the backbone, consisting of a 1,4'-bisphosphorylated β-(1→6)-linked d-glcn disaccharide with an aran residue as a non-stoichiometric substituent and a core oligosaccharide composed of kdo1h ... | 2013 | 23595053 |
| detection and diversity of aeromonads from treated wastewater and fish inhabiting effluent and downstream waters. | a two-season investigation of the wastewater treatment plant (wwtp) effluent, of related waters, sludge and fish across a wide area and 11 stations, with emphasis on aeromonas spp. was conducted. aeromonas veronii was the prevailing aeromonad isolated by maldi tof ms in the summer period. a rise of aeromonas hydrophila was observed in summer in raw sewage, treated wastewater and effluent-carrying canal. the ratio of aeromonad species retrieved from fish tissues did not correspond with the water ... | 2015 | 26092555 |
| the improved pcr of the fsta (ferric siderophore receptor) gene differentiates the fish pathogen aeromonas salmonicida from other aeromonas species. | the members of the genus aeromonas are autochthonous of aquatic ecosystems and several species have been associated to septicaemia, ulcerative and haemorrhagic diseases in fish, causing significant mortality in both wild and farmed, freshwater and marine fish species. the species aeromonas salmonicida is generally recognized as the most important fish pathogen responsible for epidemic outbreaks of furunculosis in salmonids, also being able to produce infections in other cultured fish such as tur ... | 2013 | 23890674 |
| impact of stress on aeromonas diversity in tambaqui (colossoma macropomum) and lectin level change towards a bacterial challenge. | tambaqui (colossoma macropomum) is among the most cultivated fish species in tropical countries. stress is the main cause of disease in fish farms. the genus aeromonas is a common causative agent of fish diseases. this work reports the identification of aeromonas species colonizing gills of c. macropomum submitted or not to a confinement stress. we also evaluated changes in serum levels of lectins (carbohydrate-binding proteins that are components of fish immune system) in tambaqui submitted to ... | 2016 | 27046480 |
| genotypic and phenotypic identification of aeromonas species and cpha-mediated carbapenem resistance in queensland, australia. | infection caused by aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. carbapenem resistance due to metallo-beta-lactamase, cpha encoded by the cpha gene, is a significant problem. this study defines aeromonas spp. causing clinical disease in queensland, australia. phenotypic tests for carbapenemase detection were assessed. one hundred aeromonas isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2), and sputum (1) were characterized by ... | 2016 | 26971634 |
| direct evidence of recombination in the reca gene of aeromonas bestiarum. | two hundred and twenty-one strains representative of all aeromonas species were characterized using the reca gene sequence, assessing its potential as a molecular marker for the genus aeromonas. the inter-species distance values obtained demonstrated that reca has a high discriminatory power. phylogenetic analysis, based on full-length gene nucleotide sequences, revealed a robust topology with clearly separated clusters for each species. the maximum likelihood tree showed the aeromonas bestiarum ... | 2016 | 26934994 |
| succession of lignocellulolytic bacterial consortia bred anaerobically from lake sediment. | anaerobic bacteria degrade lignocellulose in various anoxic and organically rich environments, often in a syntrophic process. anaerobic enrichments of bacterial communities on a recalcitrant lignocellulose source were studied combining polymerase chain reaction-denaturing gradient gel electrophoresis, amplicon sequencing of the 16s rrna gene and culturing. three consortia were constructed using the microbiota of lake sediment as the starting inoculum and untreated switchgrass (panicum virgatum) ... | 2016 | 26875750 |
| antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia). | antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ... | 2015 | 25803299 |
| phylogenetic diversity, antibiotic resistance and virulence traits of aeromonas spp. from untreated waters for human consumption. | it is well known that water constitutes an important contamination route for microorganisms. this is especially true for aeromonas which are widespread in untreated and treated waters. in this study, portuguese untreated waters not regularly monitored were screened for the presence and diversity of aeromonads. a total of 206 isolates were discriminated by rapd-pcr and 80 distinct strains were identified by gyrb based phylogenetic analysis. the most frequently detected species were aeromonas hydr ... | 2012 | 23107502 |
| using phenotype microarrays in the assessment of the antibiotic susceptibility profile of bacteria isolated from wastewater in on-site treatment facilities. | the scope of the study was to apply phenotype biolog microarray (pm) technology to test the antibiotic sensitivity of the bacterial strains isolated from on-site wastewater treatment facilities. in the first step of the study, the percentage values of resistant bacteria from total heterotrophic bacteria growing on solid media supplemented with various antibiotics were determined. in the untreated wastewater, the average shares of kanamycin-, streptomycin-, and tetracycline-resistant bacteria wer ... | 2017 | 28451946 |
| synthesis of the repeating unit of o-specific polysaccharide isolated from the water-borne bacteria aeromonas bestiarum 207. | aeromonas bestiarum 207 is a bacterial pathogen with severe impact on aquaculture. in a recent study, the structure of ops antigens from aeromonas bestiarum was identified as pentasaccharide repeating units. synthesis of the pentasaccharide repeating unit and its derivative are reported. stereo- and regio-specific synthesis was achieved under schmidt glycosylation conditions employing appropriately protected l-rhamopyranosyl and d-glucopyranosylamine building blocks. the pentasaccharide synthesi ... | 2017 | 29245137 |