| novel derivatives of chitosan and their antifungal activities in vitro. | three kinds of schiff bases of carboxymethyl chitosan (cmcts) were prepared, and their antifungal activities were assessed according to jasso de rodríguez's method. the results indicated that 2-(2-hydroxybenzylideneamino)-6-carboxymethylchitosan (hncmcts) and 2-(5-chloro-2-hydroxybenzylideneamino)-6-carboxymethylchitosan (hccmcts) had better inhibitory effects than those of chitosan or cmcts against fusarium oxysporium f. sp. vasinfectum, alternaria solani, and valsa mali. | 2006 | 16343460 |
| a novel and exploitable antifungal peptide from kale (brassica alboglabra) seeds. | the aim of this study was to purify and characterize antifungal peptides from kale seeds in view of the paucity of information on antifungal peptides from the family brassicaceae, and to compare its characteristics with those of published brassica antifungal peptides. a 5907-da antifungal peptide was isolated from kale seeds. the isolation procedure comprised affinity chromatography on affi-gel blue gel, ion exchange chromatography on sp-sepharose and mono s, and gel filtration on superdex pepti ... | 2008 | 18597893 |
| isolation and characterization of a lectin with potentially exploitable activities from caper (capparis spinosa) seeds. | a dimeric 62-kda lectin exhibiting a novel n-terminal amino acid sequence was purified from caper (capparis spinosa) seeds. the purification protocol involved anion-exchange chromatography, cation-exchange chromatography and, finally, gel filtration by fplc on superdex 75. approx. 100-fold purification was achieved. the haemagglutinating activity of the lectin, which was stable in the ph range 1-12 and up to 40 degrees c, could be inhibited by d(+) galactose, alpha-lactose, raffinose and rhamnos ... | 2009 | 18847434 |
| a protein with antiproliferative, antifungal and hiv-1 reverse transcriptase inhibitory activities from caper (capparis spinosa) seeds. | a protein exhibiting an n-terminal amino acid sequence with some similarity to imidazoleglycerol phosphate synthase was purified from fresh capparis spinosa melon seeds. the purification protocol entailed anion exchange chromatography on deae-cellulose, cation exchange chromatography on sp-sepharose, and finally gel filtration by fast protein liquid chromatography on superdex 75. the protein was adsorbed using 20 mm tris-hcl buffer (ph 7.4) and desorbed using 1 m nacl in the starting buffer from ... | 2009 | 19019643 |
| brassiparin, an antifungal peptide from brassica parachinensis seeds. | to isolate and characterize an antifungal peptide from the seeds of brassica parachinensis l.h.bailey. | 2009 | 19200321 |
| isolation and characterization of a french bean hemagglutinin with antitumor, antifungal, and anti-hiv-1 reverse transcriptase activities and an exceptionally high yield. | a dimeric 64-kda hemagglutinin was isolated with a high yield from dried phaseolus vulgaris cultivar "french bean number 35" seeds using a chromatographic protocol that involved blue-sepharose, q-sepharose, and superdex 75. the yield was exceptionally high (1.1g hemagglutinin per 100g seed), which is around 10-85 times higher than other phaseolus cultivars. its n-terminal sequence resembled those of other phaseolus hemagglutinins. the hemagglutinating activity of the hemagglutinin was stable in ... | 2010 | 19740639 |
| acaconin, a chitinase-like antifungal protein with cytotoxic and anti-hiv-1 reverse transcriptase activities from acacia confusa seeds. | from the seeds of acacia confusa, a chitinase-like antifungal protein designated as acaconin that demonstrated antifungal activity toward rhizoctonia solani with an ic₅₀ of 30±4 µm was isolated. acaconin demonstrated an n-terminal sequence with pronounced similarity to chitinases and a molecular mass of 32 kda. it was isolated by chromatography on q-sepharose, sp-sepharose and superdex 75 and was not bound by either ion exchanger. acaconin was devoid of chitinase activity. the antifungal activit ... | 2010 | 20725649 |
| cordymin, an antifungal peptide from the medicinal fungus cordyceps militaris. | cordymin, an antifungal peptide with a molecular mass of 10,906 da and an n-terminal amino acid sequence distinct from those of previously reported proteins, was purified from the medicinal mushroom cordyceps militaris. the isolation protocol comprised ion exchange chromatography of the aqueous extract on sp-sepharose and mono s and gel filtration on superdex 75 by a fast protein liquid chromatography system. cordymin was adsorbed on both cation exchangers. the peptide inhibited mycelial growth ... | 2011 | 20739167 |
| purification and modes of antifungal action by vicia faba cv. egypt trypsin inhibitor. | a new 15 kda bowman-birk type trypsin inhibitor (termed vfti-e1) from fava beans (vicia faba cv. egypt 1) was isolated using liquid chromatography. though it exhibited substantial homology in n-terminal amino acid sequence to other protease inhibitors, vfti-e1 showed antiproteolytic activity against trypsin (k(i) 11.9 × 10(-9) m) but hardly any activity against chymotrypsin. it demonstrated antifungal activity toward the filamentous fungus valsa mali with an ic(50) of 20 μm. the mechanism of its ... | 2010 | 20836498 |
| first report of an anti-tumor, anti-fungal, anti-yeast and anti-bacterial hemolysin from albizia lebbeck seeds. | a monomeric 5.5-kda protein with hemolytic activity toward rabbit erythrocytes was isolated from seeds of albizia lebbeck by using a protocol that involved ion-exchange chromatography on q-sepharose and sp-sepharose, hydrophobic interaction chromatography on phenyl-sepharose, and gel filtration on superdex 75. it was unadsorbed on both q-sepharose and sp-sepharose, but adsorbed on phenyl-sepharose. its hemolytic activity was fully preserved in the ph range 0-14 and in the temperature range 0-100 ... | 2011 | 20850957 |
| phytotoxic and antifungal metabolites from curvularia sp. fh01 isolated from the gut of atractomorpha sinensis. | two main phytotoxic and antifungal phthalic acid butyl isobutyl ester (1) and radicinin (2) were isolated from the culture of curvularia sp. fh01, a fungus residing in the atractomorpha sinensis gut. the structures of isolated metabolites were established on the basis of spectral analysis. metabolites 1 and 2 exhibited significant phytotoxic activity against the radical growth of echinochloa crusgalli with their ic(50) values of 61.9 and 5.9 μg/ml, respectively, which were comparable to that 2,4 ... | 2010 | 21035325 |
| purification and characterization of a cktlp protein from cynanchum komarovii seeds that confers antifungal activity. | cynanchum komarovii al iljinski is a desert plant that has been used as analgesic, anthelminthic and antidiarrheal, but also as a herbal medicine to treat cholecystitis in people. we have found that the protein extractions from c. komarovii seeds have strong antifungal activity. there is strong interest to develop protein medication and antifungal pesticides from c. komarovii for pharmacological or other uses. | 2011 | 21364945 |
| re-evaluation of pathogens causing valsa canker on apple in china. | valsa canker is a destructive disease on apple that causes serious economic losses in eastern asia. in the present study fungal isolates from cankered apple and pear bark were examined and compared with morphology and rdna-its sequences. valsa mali was confirmed to be an independent species and a causal pathogen of valsa canker on apple and pear in china. it was the predominant species (96.7% of isolates) on apple and was complemented by v. malicola (3.3% of isolates). significant intraspecific ... | 2011 | 21415290 |
| [development of genetic transformation system of valsa mali of apple mediated by peg]. | the genetic transformation of valsa mali var. mali was developed by peg-mediated protoplasts transformation. | 2011 | 22126074 |