| classification of ralstonia pickettii biovar 3/'thomasii' strains (pickett 1994) and of new isolates related to nosocomial recurrent meningitis as ralstonia mannitolytica sp. nov. | strains isolated independently from two patients could be recognized as ralstonia pickettii biovar 3/'thomasii'. the 16s rdna sequences of these strains and two other strains of r. pickettii biovar 3/'thomasii' clustered at less than 98% similarity versus all other described ralstonia species and at less than 97 % versus the two other r. pickettii biovars. the separate species status of r. pickettii biovar 3/'thomasii' was confirmed by dna-dna hybridization, indicating less than 60% dna homology ... | 2001 | 11321101 |
| one case each of recurrent meningitis and hemoperitoneum infection with ralstonia mannitolilytica. | two clinical cases of infection with ralstonia mannitolilytica are described: a recurrent meningitis on an implanted intraventricular catheter and an infected hemoperitoneum as a complication of a cholangiocarcinoma. the strains were first misidentified as pseudomonas fluorescens and burkholderia cepacia. further testing lead to the identification as ralstonia pickettii biovar 3/"thomasii," which was recently shown to represent a separate species, r. mannitolilytica (list editor n. weiss, int. j ... | 2001 | 11724893 |
| characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of inquilinus limosus gen. nov., sp. nov. | using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16s ribosomal dna sequencing, and dna-dna hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (cf) patients. our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from cf patients. these taxa include acinetobacter sp., bordetella hinzii, burkholderia fungorum, comamonas testost ... | 2002 | 12037065 |
| use of 16s rrna gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center. | during 1999, we used partial 16s rrna gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. these 46 isolates were genotypically identified as pseudomonas aeruginosa (19 isolates, 12 patients), achromobacter xylosoxidans (10 isolates, 8 ... | 2002 | 12354883 |
| use of amplified ribosomal dna restriction analysis for identification of ralstonia and pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis. | the recovery of ralstonia and pandoraea species from respiratory tract cultures of patients with cystic fibrosis has recently been reported. these species are difficult to identify, and especially to differentiate from burkholderia cepacia complex organisms, with classical methods. the discriminatory power of amplified ribosomal dna restriction analysis (ardra) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six en ... | 2003 | 12843108 |
| [phenotypic and genotypic characteristics of non fermenting atypical strains recovered from cystic fibrosis patients]. | we used partial 16s rrna gene (16s dna) sequencing for the prospective identification of nonfermenting gram-negative bacilli recovered from patients attending our cystic fibrosis center (hôpital necker-enfants malades), which gave problematic results with conventional phenotypic tests. during 1999, we recovered 1093 isolates of nonfermenting gram-negative bacilli from 702 sputum sampled from 148 patients. forty-six of these isolates (27 patients) were not identified satisfactorily in routine lab ... | 2003 | 12948761 |
| wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including ralstonia eutropha and related species, and proposal of ralstonia [pseudomonas] syzygii (roberts et al. 1990) comb. nov. | comparative 16s rdna sequence analysis indicates that two distinct sublineages, with a sequence dissimilarity of >4 % (bootstrap value, 100 %), exist within the genus ralstonia: the ralstonia eutropha lineage, which comprises ralstonia basilensis, ralstonia campinensis, r. eutropha, ralstonia gilardii, ralstonia metallidurans, ralstonia oxalatica, ralstonia paucula, ralstonia respiraculi and ralstonia taiwanensis; and the ralstonia pickettii lineage, which comprises ralstonia insidiosa, ralstoni ... | 2004 | 15023939 |
| characterization of clinically isolated ralstonia mannitolilytica strains using random amplification of polymorphic dna (rapd) typing and antimicrobial sensitivity, and comparison of the classification efficacy of phenotypic and genotypic assays. | ralstonia mannitolilytica strains isolated between february 2002 and march 2004 from 30 episodes of infection in 26 patients at vienna university hospital were characterized. twenty-four of the episodes occurred within a 7 month period, suggesting they were outbreak-related, although no common source of infection was identified. the isolates were assayed using pcr to confirm species identification. random amplification of polymorphic dna (rapd) typing classified the r. mannitolilytica isolates i ... | 2005 | 15591256 |
| use of pcr analyses to define the distribution of ralstonia species recovered from patients with cystic fibrosis. | two new pcr assays (for ralstonia species and ralstonia respiraculi), together with previously published pcr assays, were used to assess ralstonia isolates recovered from 111 cystic fibrosis patients. ralstonia mannitolilytica accounted for 46% of isolates, while r. respiraculi and ralstonia pickettii accounted for 19% and 18%, respectively. ralstonia basilensis and ralstonia metallidurans, species not previously recovered from human samples, were also identified. | 2005 | 16000479 |
| [use of 16s rrna gene sequencing for identification of "pseudomonas-like" isolates from sputum of patients with cystic fibrosis]. | since nonfermenting, gram negative bacilli recovered from patients with cystic fibrosis could be misidentified with phenotypic procedures, we used partial 16s ribosomal rna gene (16s gene) sequencing to identify these "pseudomonas-like" isolates. 473 isolates were recovered from 66 patients in 2003. sequencing was used to identify 29 (from 24 patients) of the 473 isolates, showing unclear results with routine tests. pcr with specific primers was carried out to amplify a 995 bp fragment, which wa ... | 2005 | 16081224 |
| coselection for microbial resistance to metals and antibiotics in freshwater microcosms. | bacterial resistances to diverse metals and antibiotics are often genetically linked, suggesting that exposure to toxic metals may select for strains resistant to antibiotics and vice versa. to test the hypothesis that resistances to metals and antibiotics are coselected for in environmental microbial assemblages, we investigated the frequency of diverse resistances in freshwater microcosms amended with cd, ni, ampicillin or tetracycline. we found that all four toxicants significantly increased ... | 2006 | 16913911 |
| a national outbreak of ralstonia mannitolilytica associated with use of a contaminated oxygen-delivery device among pediatric patients. | in august 2005, the centers for disease control and prevention was notified of a ralstonia species outbreak among pediatric patients receiving supplemental oxygen therapy with the vapotherm 2000i (vapotherm, inc, stevensville, md). the vapotherm 2000i is a reusable medical device that was used in >900 hospitals in the united states in 2005. ralstonia are waterborne bacilli that have been implicated in hospital-acquired infections. we initiated an investigation to determine the source of the outb ... | 2007 | 17545371 |
| providing a safety net for children: investigating a multistate outbreak of ralstonia mannitolilytica related to a contaminated reusable device. | | 2007 | 17545391 |
| ralstonia mannitolilytica infection in renal transplant recipient: first report. | ralstonia mannitolilytica is being increasingly identified as an opportunist pathogen in immunocompromised patients. we report the first case of post renal transplant infection by r. mannitolilytica, in a 14-year-old recipient. the graft and the patient were saved with prompt microbiological identification, sensitivity testing and subsequent administration of appropriate antibiotic. | 2003 | 17643046 |
| monoclonal outbreak of catheter-related bacteraemia by ralstonia mannitolilytica on two haemato-oncology wards. | ralstonia mannitolilytica is a non-fermentative, gram-negative bacterium isolated infrequently from clinical samples. however, within a period of 11 weeks five inpatients of the tertiary care hospital of the university of tübingen developed clinical signs of infection and r. mannitolilytica was cultivated from blood samples of all patients suggesting an outbreak. | 2007 | 17881058 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients. | the identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (cf) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. these techniques remain time-consuming, expensive, and technically demanding. we used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof-ms) for the identification of these bacteria. a set of reference strains belonging to 58 species of clinically relevan ... | 2008 | 18685005 |
| strains of internal biofilm in aerobic granular membrane bioreactors. | this study isolated strains in suspended liquor, the surface fouling layer, and biofilm inside hollow-fiber membranes of a membrane bioreactor (mbr); analyzed their distributions, sizes, surface charges, and growth behaviors; and determined the quantities of extracellular polymeric substances (eps) secreted by these strains under different organic loadings. three strains, which may penetrate the microfiltration membranes, were close relatives of the ralstonia mannitolilytica strain sdv (genbank ... | 2010 | 20306189 |
| identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal rna gene sequence analysis. | clin microbiol infect 2011; 17: 776-779 abstract: dna sequence-based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16s and internal transcribed spacer (its) ribosomal rna gene sequence analysis. pcr was positive for 24 of 99 samples tested. both culture and 16s rdna sequence analysis identified pseudomonas aeruginosa, streptococci and enterobacteriaceae. isolates misidentified as burkholderia cepacia by biochemical tes ... | 2011 | 21521414 |
| ralstonia mannitolilytica and copd: a case report. | | 2011 | 22130767 |
| [the first experience of application of pcr techniques in real-time mode to diagnose bacteriemia during postoperational period in cardiosurgery patients]. | the results comparison is made between pcr polymerase chain reaction techniques in real-time mode and cultural techniques of analysis of blood samples in patients with suspicion of bacteriemia during early postoperational period. from september to december 2009 43 blood samples from 37 adult patients aged 59+9.2 years were analyzed on suspicion of bacteriemia. the blood samples for pcr analysis (septifast technique roche diagnostics, switzerland) were taken parallel to the inoculation. among 43 ... | 2011 | 22164420 |
| ralstonia mannitolilytica in cystic fibrosis: a new predictor of worse outcomes. | patients with cystic fibrosis are subject to repeated respiratory tract infections, with recent increasing isolation of unusual pathogens. ralstonia species have lately been isolated at our institution, an organism historically frequently misidentified as burkholderia or pseudomonas. the prevalence of ralstonia spp. in cystic fibrosis populations has yet to be determined, along with its clinical implications. | 2016 | 27995056 |
| genome sequence of a carbapenem-resistant strain of ralstonia mannitolilytica. | ralstonia mannitolilytica, a gram-negative aerobic bacterium, is an opportunistic human pathogen that is becoming more common in cases of nosocomial infections. we report for the first time the whole-genome sequence analysis of r. mannitolilytica strain mry14-0246, which carries the intrinsic oxa-443/oxa-22-like and oxa-444/oxa-60-like β-lactamase genes and is resistant to meropenem. | 2015 | 25953190 |
| ralstonia picketti neonatal sepsis: a case report. | ralstonia genus are gram negative bacillus and includes four bacteria namely ralstonia picketti, ralstonia solanacearum, ralstonia insidiosa and ralstonia mannitolilytica. these are opportunistic pathogens and cause infections in immunocompromised host. the sources of infection are usually contaminated solutions and water. the majority of the reported cases are caused by r. picketti. it is very rare cause of neonatal sepsis with less than twenty cases reported in literature till date. | 2017 | 28061799 |
| development of an in vitro assay, based on the biofilm ring test(®), for rapid profiling of biofilm-growing bacteria. | microbial biofilm represents a major virulence factor associated with chronic and recurrent infections. pathogenic bacteria embedded in biofilms are highly resistant to environmental and chemical agents, including antibiotics and therefore difficult to eradicate. thus, reliable tests to assess biofilm formation by bacterial strains as well as the impact of chemicals or antibiotics on biofilm formation represent desirable tools for a most effective therapeutic management and microbiological risk ... | 2016 | 27708625 |
| ralstonia mannitolilytica infections in an oncologic day ward: description of a cluster among high-risk patients. | ralstonia spp, an environmental microorganism, has been occasionally associated with healthcare infections. the aim of this study was to investigate an outbreak caused by ralstonia mannitolilytica in oncology patients. | 2017 | 28191308 |
| ralstonia mannitolilytica-induced septicemia and homology analysis in infected patients: 3 case reports. | ralstonia mannitolilytica is an emerging opportunistic pathogen. hospital outbreaks of ralstonia spp. are mainly associated with contaminated treatment water or auxiliary instruments. | 2016 | 27679705 |
| ralstonia spp.: emerging global opportunistic pathogens. | the bacterial genus ralstonia (gram-negative non-fermenters) is becoming more prevalent in cases of infection with three bacterial species, ralstonia pickettii, ralstonia insidiosa and ralstonia mannitolilytica, making up all cases reported (in the literature) to date. these organisms are prevalent in many different types of water supplies (including hospital water supplies), being well adapted to survive in low-nutrient conditions. they have been shown to cause infections, sometimes serious, su ... | 2014 | 24057141 |
| déjà vu: ralstonia mannitolilytica infection associated with a humidifying respiratory therapy device, israel, june to july 2011. | following a bloodstream infection in june 2011 with ralstonia mannitolilytica in a premature infant treated with a humidifying respiratory therapy device, an investigation was initiated at the hadassah medical centres in jerusalem. the device delivers a warmed and humidified mixture of air and oxygen to patients by nasal cannula. the investigation revealed colonisation with r. mannitolilytica of two of 15 patients and contamination of components of five of six devices deployed in the premature u ... | 2013 | 23725776 |
| peritonitis due to ralstonia mannitolilytica in a pediatric peritoneal dialysis patient. | ralstonia mannitolilytica constitutes a rare isolate in clinical specimens and to date very few infections with this gramnegative bacillus have been reported. the first case of peritonitis in a pediatric patient due to r. mannitolilytica in the setting of peritoneal dialysis is described. it is very important to view this organism as a pathogen rather than contaminant when isolated in children with peritonitis. | 2012 | 23109020 |