| indian citrus ringspot virus: a proposed new species with some affinities to potex-, carla-, fovea- and allexiviruses. | an isolate of indian citrus ringspot virus from kinnow mandarin in northern india had flexuous particles with evident cross-banding and a modal length of 650 nm. it was mechanically transmitted to five herbaceous hosts including phaseolus vulgaris cv saxa, in which it became systemic. in thin sections, virus particles were observed in the cytoplasm of parenchyma cells but no specific inclusions were seen. the virus was purified from infected saxa bean leaves and an antiserum prepared. there was ... | 2000 | 11043949 |
| nucleotide sequence, genome organisation and phylogenetic analysis of indian citrus ringspot virus. brief report. | the sequence of the single-stranded rna genome of indian citrus ringspot virus (icrsv) consists of 7560 nucleotides. it contains six open reading frames (orfs) which encode putative proteins of 187.3, 25, 12, 6.4, 34 and 23 kda respectively. orf1 encodes a polypeptide that contains all the elements of a replicase; orfs 2, 3 and 4 compose a triple-gene block; orf5 encodes the capsid protein; the function of orf6 is unknown. phylogenetic analysis of the complete genome and each orf separately, and ... | 2002 | 12417955 |
| a multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees. | a multiplex polymerase chain reaction (mpcr) assay was developed to detect six rna and one dna citrus virus: citrus leaf rugose virus (clrv), citrus psorosis virus (cpsv), citrus tatter leaf virus (ctlv), citrus tristeza virus (ctv), citrus variegation virus (cvv), citrus yellow mosaic virus (cymv), and indian citrus ringspot virus (icrsv) from citrus plants. these seven viruses are classified in six different virus genera. degenerate primers were designed based on the respective virus isolate s ... | 2005 | 15951030 |
| development of multiplex polymerase chain reaction assay for simultaneous detection of clostero-, badna- and mandari-viruses along with huanglongbing bacterium in citrus trees. | citrus trees harbor a large number of viral and bacterial pathogens. citrus yellow vein clearing virus (cyvcv), indian citrus ringspot virus (icrsv), citrus yellow mosaic virus (cymv), citrus tristeza virus (ctv) and a bacterium, candidatus liberibacter asiaticus (cla) associated with huanglongbing (hlb) disease, the most prevalent pathogens in citrus orchards of different regions in india and are responsible for debilitating citriculture. for detection of these viral and bacterial pathogens a q ... | 2016 | 27208471 |
| the genome sequence of an isolate of indian citrus ringspot virus infecting the sweet orange in india. | whole-genome sequencing of an isolate of mandarivirus infecting the sweet orange [citrus sinensis (l) blanco] in the western part of india (pune) was done. the single-stranded positive-sense rna genome of indian citrus ringspot virus (icrsv) pune has 7,560 nucleotides (nt), excluding a poly(a) tail, comprised of 27.98% (2,115 nt) a, 32.12% (2,428 nt) c, 19.68% (1,488 nt) g, and 20.22% (1,529 nt) t residues. the genome, organized into six open reading frames (orfs), shares 97.7% sequence identity ... | 2012 | 23087111 |
| identification and characterization of citrus yellow vein clearing virus, a putative new member of the genus mandarivirus. | molecular features and genomic organization were determined for citrus yellow vein clearing virus (cyvcv), the putative viral causal agent of yellow vein clearing disease of lemon trees, reported in pakistan, india, and more recently in turkey and china. cyvcv isolate y1 from adana, turkey, was used for deep sequencing analysis of the virus-induced small rna fractions and for mechanical and graft inoculation of herbaceous and citrus indicator plants. a polyclonal antiserum was developed from cyv ... | 2012 | 22913410 |