| 3'-terminal sequence analysis of the rna genome of the indian isolate of cardamom mosaic virus: a new member of genus macluravirus of potyviridae. | cardamom mosaic virus, a possible member of the family potyviridae has been associated with the mosaic disease (katte disease) of small cardamom in india. a virus isolated from the symptomatic cardamom leaves was positive in elisa only with antiserum to the guatemalan isolate of cardamom mosaic virus and not with a number of other potyviruses. the size of the viral rna (8.5 kb) and the molecular weight of the coat protein (cp) (38 kda) were determined. a 1.8-kb product containing the partial nuc ... | 2001 | 11556406 |
| expression, purification and molecular modeling of the nia protease of cardamom mosaic virus. | the nia protease of potyviridae is the major viral protease that processes potyviral polyproteins. the nia protease coding region of cardamom mosaic virus (cdmv) is amplified from the viral cdna, cloned and expressed in escherichia coli. nia protease forms inclusion bodies in e.coli. the inclusion bodies are solubilized with 8 m urea, refolded and purified by nickel-nitrilotriacetic acid affinity chromatography. three-dimensional modeling of the cdmv nia protease is achieved by threading approac ... | 2013 | 22888800 |
| expression and immunological characterization of cardamom mosaic virus coat protein displaying hiv gp41 epitopes. | the coat protein of cardamom mosaic virus (cdmv), a member of the genus macluravirus, assembles into virus-like particles when expressed in an escherichia coli expression system. the n and c-termini of the coat protein were engineered with the kennedy peptide and the 2f5 and 4e10 epitopes of gp41 of hiv. the chimeric proteins reacted with sera from hiv positive persons and also stimulated secretion of cytokines by peripheral blood mononuclear cells from these persons. thus, a system based on the ... | 2013 | 23668610 |
| highly efficient immunodiagnosis of large cardamom chirke virus using the polyclonal antiserum against escherichia coli expressed recombinant coat protein. | large cardamom chirke virus (lccv), genus macluravirus, family potyviridae is an important constrain in large cardamom production in india. purification of lccv from large cardamom tissues is difficult and therefore immunodiagnostic reagents are not available. in the present study, we have successfully expressed coat protein (cp) gene of lccv in escherichia coli. the purification of expressed protein by ni-nta affinity chromatography was inefficient due to precipitation of protein during renatur ... | 2013 | 24426280 |
| molecular modeling and in-silico engineering of cardamom mosaic virus coat protein for the presentation of immunogenic epitopes of leptospira lipl32. | expression of cardamom mosaic virus (cdmv) coat protein (cp) in e. coli forms virus-like particles. in this study, the structure of cdmv cp was predicted and used as a platform to display epitopes of the most abundant surface-associated protein, lipl32 of leptospira at c, n, and both the termini of cdmv cp. in silico, we have mapped sequential and conformational b-cell epitopes from the crystal structure of lipl32 of leptospira interrogans serovar copenhageni str. fiocruz l1-130 using iedb elipr ... | 2016 | 25692534 |
| detection of cardamom mosaic virus and banana bract mosaic virus in cardamom using sybr green based reverse transcription-quantitative pcr. | cardamom being perennial, propagated vegetatively, detecting viruses in planting material is important to check the spread of viruses through infected material. thus development of effective and sensitive assay for detection of viruses is need of the time. in this view, assay for the detection of cardamom mosaic virus (cdmv) and banana bract mosaic virus (bbrmv), infecting cardamom was developed using sybr green one step reverse transcription-quantitative pcr (rt-qpcr). the rt-qpcr assay amplifi ... | 2014 | 24426323 |
| survey and rt-pcr based detection of cardamom mosaic virus affecting small cardamom in india. | mosaic or marble or katte disease caused by cardamom mosaic virus (cdmv) is an important production constraint in all cardamom growing regions of the world. in the present study, 84 cardamom plantations in 44 locations of karnataka and kerala were surveyed. the incidence of the disease ranged from 0 to 85%. the incidence was highest in madikeri (karnataka) while no incidence was recorded in peermade (kerala). in general, incidence and severity of the disease was higher in cardamom plantations of ... | 2011 | 23637495 |
| molecular modeling and conformational analysis of native and refolded viral genome-linked protein of cardamom mosaic virus. | the viral genome-linked protein (vpg) of potyviruses is covalently attached to the 5' end of the genomic rna. towards biophysical characterization, the vpg coding region of cardamom mosaic virus (cdmv) was amplified from the cdna and expressed in e. coli. most of the expressed vpg aggregated as inclusion bodies that were solubilized with urea and refolded with l-arginine hydrochloride. the various forms of cdmv vpg (native, denatured and refolded) were purified and the conformational variations ... | 2011 | 22165292 |
| optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus. | all rna viruses encode an rna-dependent rna polymerase (rdrp) that is required for replication of the viral genome. nuclear inclusion b (nib) gene codes for the rdrp in potyviridae viruses. in this study, expression, solubilization and purification of nib protein of cardamom mosaic virus (cdmv) is reported. the objective of the present study was to express and purify the nib protein of cdmv on a large scale for structural characterization, as the structure of the rdrp from a plant virus is yet t ... | 2008 | 21086722 |
| high genetic diversity in the coat protein and 3 untranslated regions among geographical isolates of cardamom mosaic virus from south india. | a survey was conducted to study the biological and genetic diversity of cardamom mosaic virus (cdmv) that causes the most widespread disease in the cardamom growing area in the western ghats of south india. six distinct subgroups were derived based on their symptomatology and host range from the sixty isolates collected. the serological variability between the virus isolates was analysed by elisa and western blotting. the 3 terminal region consisting of the coat protein (cp) coding sequence and ... | 2003 | 14517362 |
| molecular evidence that aphid-transmitted alpinia mosaic virus is a tentative member of the genus macluravirus. | alpinia mosaic virus (alpmv), once assigned to the genus potyvirus, infects primarily plants of the ginger family. to seek molecular evidence for correct classification of this virus, a cdna clone corresponding to the 3' portion of the alpmv genome was obtained by reverse transcriptase-pcr and ta cloning. the authenticity of the cdna clone was confirmed by expression of the coat protein (cp) in e. coli followed by immunoblot analysis. sequence analysis indicated that, in contrast to its low iden ... | 2003 | 12756625 |
| expression of cardamom mosaic virus coat protein in escherichia coli and its assembly into filamentous aggregates. | cardamom mosaic virus (cdmv), a member of the genus macluravirus of potyviridae, causes a mosaic disease in cardamom. a polyclonal antiserum was raised against the purified virus and igg was prepared. electron microscopic studies on the purified virus showed flexuous filamentous particles of approximately 800 nm in length, typical of members of potyviridae. the coat protein (cp) encoding sequence of the virus was expressed in escherichia coli and the protein purified by affinity chromatography u ... | 2002 | 12076837 |