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characterization of a sapstaining fungus, ophiostoma floccosum, isolated from the sapwood of pinus thunbergii in korea.an ophiostoma fungus was isolated from a stump of pinus thunbergii in a forest on the west coast of korea. microscopic analysis using a light microscope, a stereo microscope, and a scanning electron microscope revealed that it had morphological features of pesotum and sporothix synanarmorphs. based on the β-tubulin gene sequence analysis, the fungus was identified as the anamorph of ophiostoma floccosum. mycological properties of the species including its growth properties on different culture m ...200923983499
quorum sensing activity and control of yeast-mycelium dimorphism in ophiostoma floccosum.quorum sensing (qs) activity in ophiostoma fungi has not been described. we have examined the growth conditions on the control of dimorphism in ophiostoma floccosum, an attractive biocontrol agent against blue-stain fungi, and its relationship with qs activity. in a defined culture medium with l-proline as the n source, a high inoculum size (10(7) c.f.u. ml(-1)) was the principal factor that promoted yeast-like growth. inoculum size effect can be explained by the secretion of a qs molecule(s) (q ...201424737073
large shift in symbiont assemblage in the invasive red turpentine beetle.changes in symbiont assemblages can affect the success and impact of invasive species, and may provide knowledge regarding the invasion histories of their vectors. bark beetle symbioses are ideal systems to study changes in symbiont assemblages resulting from invasions. the red turpentine beetle (dendroctonus valens) is a bark beetle species that recently invaded china from its native range in north america. it is associated with ophiostomatalean fungi in both locations, although the fungi have ...201324205124
improvement of the secretion of extracellular proteins and isolation and characterization of the amylase i (amy1) gene from ophiostoma floccosum.uv mutagenesis was applied to improve protein secretion in ophiostoma floccosum. amylase activity was used as an indicator for enhanced protein production after repeated rounds of mutagenic treatment. the amylase activity in the culture supernatant of the best mutant (mq.5.1) was increased by more than 240-fold compared to the initial parental strain. at the same time, the increase in total secreted protein was about six times greater than the parental strain. secreted proteinase and lipase acti ...200616971061
assessing rnai frequency and efficiency in ophiostoma floccosum and o. piceae.ophiostoma species are an economically important group of saprophytic and pathogenic fungi that grow in trees or wood. ophiostoma like o. piceae and o. floccosum produce melanin, a pigment that stains lumber and logs. we used such species as model organisms for characterizing the molecular mechanisms in fungal melanin production. because homologous recombination is rare in the ophiostoma, identifying gene function in this group is challenging. we addressed this by assessing rna interference (rna ...200616859936
a second reductase gene involved in melanin biosynthesis in the sap-staining fungus ophiostoma floccosum.two different dna sequences amplified by pcr from ophiostoma floccosum show high homology to reductase genes of the fungal melanin biosynthetic pathway and suggest that two reductases may participate in this pathway in o. floccosum. previously, one reductase gene, thn1 had been isolated from o. floccosum and proved to be involved in melanin biosynthesis. the second reductase gene, thn2, encodes a protein of 284 amino acids and is the longest fungal melanin reductase so far reported. the protein ...200212172794
cloning and genetic analysis of subtilases in sapstaining fungi.in order to assess the genetic variance of a group of homologous subtilases in sapstaining fungi, molecular techniques were employed. first, pcr screening with degenerate primers and dot-blot analyses were used to screen 31 different isolates, representing nine species, of sapstaining fungi for the presence of subtilase-like sequences. restriction fragment length polymorphism pcr and sequence analysis techniques were then used to determine the inter- and intraspecies variation of these genes. a ...200212111098
a scytalone dehydratase gene from ophiostoma floccosum restores the melanization and pathogenicity phenotypes of a melanin-deficient colletotrichum lagenarium mutant.scytalone dehydratase is involved in the production of fungal dihydroxynaphthalene (dhn) melanin. we have isolated and characterized osd1, a gene encoding scytalone dehydratase from the sap-staining fungus ophiostoma floccosum by pcr-based cloning. sequence analysis suggests that the osd1 gene encodes a protein of 216 amino acids with a molecular weight of 24.2 kda that shows 51-70% sequence identity to other scytalone dehydratases. the cloned osd1 contains two introns of 76 bp and 63 bp in leng ...200111589570
fungal associates of the lodgepole pine beetle, dendroctonus murrayanae.bark beetles are well known vectors of ophiostomatoid fungi including species of ophiostoma, grosmannia and ceratocystis. in this study, the most common ophiostomatoid fungi associated with the lodgepole pine beetle, dendroctonus murrayanae, were characterized. pre-emergent and post-attack adult beetles were collected from lodgepole pines at four sites in british columbia, canada. fungi were isolated from these beetles and identified using a combination of morphology and dna sequence comparisons ...201121553309
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