| description of cellulophaga baltica gen. nov., sp. nov. and cellulophaga fucicola gen. nov., sp. nov. and reclassification of [cytophaga] lytica to cellulophaga lytica gen. nov., comb. nov. | phenotypic data indicate that gliding, yellow/orange-pigmented, agar-digesting bacterial strains were members of the cytophaga-flavobacterium-bacteroides (cfb) group. the strains were isolated from the surface of the marine benthic macroalga fucus serratus l. and the surrounding seawater at three localities in danish waters. the bacteria were gram-negative, flexirubin-negative, aerobic, catalase-positive and oxidase-negative and were psychrophilic and halophilic. all strains utilized d-fructose, ... | 1999 | 10425785 |
| description of cellulophaga algicola sp. nov., isolated from the surfaces of antarctic algae, and reclassification of cytophaga uliginosa (zobell and upham 1944) reichenbach 1989 as cellulophaga uliginosa comb. nov. | a group of strains with potent extracellular enzymic activity were isolated from the surfaces of the chain-forming sea-ice diatom melosira and from an unidentified macrophyte collected from the eastern antarctic coastal zone. 16s rdna sequence analysis indicated that the strains belonged to the genus cellulophaga and showed greatest similarity to the species cellulophaga baltica (sequence similarity 97%). phenotypic characteristics, dna base composition and dna-dna hybridization values clearly s ... | 2000 | 11034497 |
| polyamine distribution profiles in newly validated genera and species within the flavobacterium-flexibacter-cytophaga-sphingobacterium complex. | cellular polyamines of 58 strains belonging to the flavobacterium-flexibacter-cytophaga-sphingobacterium complex were analysed by hplc. homospermidine was found in all species of flavobacterium, chryseobacterium, empedobacter, myroides, cellulophaga, salegentibacter, psychroserpens and gelidibacter of the family flavobacteriaceae. flavobacterium ferrugineum located outside of this family also contained homospermidine. cytophaga fermentans and c. xylanolytica belonging to the family bacteroidacea ... | 2001 | 11548199 |
| cellulophaga pacifica sp. nov. | three marine, heterotrophic, aerobic, agarolytic, pigmented and gliding bacteria were isolated in june 2000 from a sea water sample that was collected in the gulf of peter the great, sea of japan, and analysed in a polyphasic taxonomic study. 16s rdna sequence analysis indicated that strains kmm 3664(t), kmm 3669 and kmm 3915 were members of the family flavobacteriaceae. based on phenotypic, chemotaxonomic, genotypic and phylogenetic data, the isolates were classified in the genus cellulophaga a ... | 2004 | 15023983 |
| distribution of two triamines, spermidine and homospermidine, and an aromatic amine, 2-phenylethylamine, within the phylum bacteroidetes. | cellular polyamines of the newly additional 19 species belonging to the class bacteroides of the phylum bacteroidetes were analyzed by hplc to display polyamine distribution as a chemotaxonomic marker within the total 41 species. three profiles, the presence of spermidine, the presence of homospermidine and the absence of both triamines, corresponded to their phylogenetical positions within the four families of the class. the occurrence of an aromatic amine, 2-phenylethylamine, extracted into ce ... | 2004 | 15747230 |
| dokdonia donghaensis gen. nov., sp. nov., isolated from sea water. | two gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strains, dsw-1t and dsw-21, were isolated from sea water and subjected to a polyphasic taxonomic study. they grew optimally at 30 degrees c and in the presence of 2 % (w/v) nacl. strains dsw-1t and dsw-21 were characterized chemotaxonomically as containing mk-6 as the predominant menaquinone and iso-c15 : 0, iso-c17 : 0 3-oh and iso-c15 : 1 as the major fatty acids. their dna g+c content was 38 mol%. strains dsw-1t a ... | 2005 | 16280490 |
| krokinobacter gen. nov., with three novel species, in the family flavobacteriaceae. | five strains belonging to the family flavobacteriaceae were isolated from marine-sediment samples collected in sagami and tokyo bays on the pacific coastline of japan. the five isolates formed a coherent and novel genus-level lineage within the family flavobacteriaceae. the most closely related species with a validly published name was cellulophaga lytica. the five isolates were rod-shaped, gram-negative, aerobic, catalase- and oxidase-positive, flexirubin-negative and yellow-pigmented. the domi ... | 2006 | 16449433 |
| predominance of roseobacter, sulfitobacter, glaciecola and psychrobacter in seawater collected off ushuaia, argentina, sub-antarctica. | bacterial diversity in sub-antarctic seawater, collected off ushuaia, argentina, was examined using a culture independent approach. the composition of the 16s rrna gene libraries from seawater and seawater contaminated with the water soluble fraction of crude oil was statistically different (p value 0.001). in both libraries, clones representing the alphaproteobacteria, gammaproteobacteria, the cytophaga-flavobacterium-bacteroidetes group and unculturable bacteria were dominant. clones associate ... | 2007 | 17026513 |
| [structure of acidic o-specific polysaccharide from the marine bacterium cellulophaga baltica]. | the structure of an acidic o-specific polysaccharide from the marine bacterium cellulophaga baltica was established by chemical methods of analysis and nmr spectroscopy. the polysaccharide was shown to consist of repeating tetrasaccharide units containing two mannose residues, one n-acetyl-d-glucosamine residue, and one d-glucuronic acid residue. an o-acetyl group was also found in the polysaccharide in nonstoichiometric amount. thus, this polysaccharide had the following structure: [carbohydrat ... | 2007 | 17375664 |
| a pseudoaminic acid-containing o-specific polysaccharide from a marine bacterium cellulophaga fucicola. | the o-polysaccharide was isolated from the lipopolysaccharide of cellulophaga fucicola and studied by chemical analyses along with (1)h and (13)c nmr spectroscopy. the following new structure of the o-polysaccharide of c. fucicola containing 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid residue (pseudoaminic acid, psep) was elucidated as the following: | 2007 | 17482585 |
| large variabilities in host strain susceptibility and phage host range govern interactions between lytic marine phages and their flavobacterium hosts. | phages are a main mortality factor for marine bacterioplankton and are thought to regulate bacterial community composition through host-specific infection and lysis. in the present study we demonstrate for a marine phage-host assemblage that interactions are complex and that specificity and efficiency of infection and lysis are highly variable among phages infectious to strains of the same bacterial species. twenty-three bacteroidetes strains and 46 phages from swedish and danish coastal waters ... | 2007 | 17766444 |
| low densities of epiphytic bacteria from the marine alga ulva australis inhibit settlement of fouling organisms. | bacteria that produce inhibitory compounds on the surface of marine algae are thought to contribute to the defense of the host plant against colonization of fouling organisms. however, the number of bacterial cells necessary to defend against fouling on the plant surface is not known. pseudoalteromonas tunicata and phaeobacter sp. strain 2.10 (formerly roseobacter gallaeciensis) are marine bacteria often found in association with the alga ulva australis and produce a range of extracellular inhib ... | 2007 | 17965210 |
| elevated lytic phage production as a consequence of particle colonization by a marine flavobacterium (cellulophaga sp.). | bacteria growing on marine particles generally have higher densities and cell-specific activities than free-living bacteria. since rapidity of phage adsorption is dependent on host density, while infection productivity is a function of host physiological status, we hypothesized that marine particles are sites of elevated phage production. in the present study, organic-matter-rich agarose beads and a marine phage-host pair (cellulophaga sp., phis(m)) were used as a model system to examine whether ... | 2008 | 18347846 |
| combinatorial materials research applied to the development of new surface coatings ix: an investigation of novel antifouling/fouling-release coatings containing quaternary ammonium salt groups. | polysiloxane coatings containing chemically-bound ("tethered") quaternary ammonium salt (qas) moieties were investigated for potential application as environmental-friendly coatings to control marine biofouling. a combinatorial/high-throughput approach was applied to the investigation to enable multiple variables to be probed simultaneously and efficiently. the variables investigated for the moisture-curable coatings included qas composition, ie alkyl chain length, and concentration as well as s ... | 2008 | 18368587 |
| molecular structure of endotoxins from gram-negative marine bacteria: an update. | marine bacteria are microrganisms that have adapted, through millions of years, to survival in environments often characterized by one or more extreme physical or chemical parameters, namely pressure, temperature and salinity. the main interest in the research on marine bacteria is due to their ability to produce several biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents. nonetheless, lipopolysaccharides (lpss), or their portions, from g ... | 2007 | 18463721 |
| cellulophaga tyrosinoxydans sp. nov., a tyrosinase-producing bacterium isolated from seawater. | an aerobic, gliding, yellow-pigmented bacterium lacking flagella and showing strong tyrosinase activity, designated strain em41(t), was isolated from seawater on the eastern coast of jeju island in korea. growth was observed at 15-35 degrees c (optimum, 25-30 degrees c) and at ph 6.5-9.0 (optimum, ph 7.0-8.5). cells were gram-negative, negative for flexirubin pigments and catalase- and oxidase-positive. the g+c content of the genomic dna was 33.5 mol% and the major respiratory quinone was menaqu ... | 2009 | 19329582 |
| bacteriophages drive strain diversification in a marine flavobacterium: implications for phage resistance and physiological properties. | genetic, structural and physiological differences between strains of the marine bacterium cellulophaga baltica mm#3 (flavobacteriaceae) developing in response to the activity of two virulent bacteriophages, phi s(m) and phi s(t), was investigated during 3 weeks incubation in chemostat cultures. a distinct strain succession towards increased phage resistance and a diversification of the metabolic properties was observed. during the incubation the bacterial population diversified from a single str ... | 2009 | 19508553 |
| lutaonella thermophila gen. nov., sp. nov., a moderately thermophilic member of the family flavobacteriaceae isolated from a coastal hot spring. | a yellow-pigmented, gram-staining-negative, aerobic, non-motile, moderately thermophilic, rod-shaped bacterium, cc-mhsw-2t, was isolated from a coastal hot spring of green island (lutao), located off taituang, taiwan. the 16s rrna gene sequence analysis demonstrated that it shared <93.2% sequence similarity with aquimarina species. the organism was unable to produce acid from carbohydrates, but it could utilize a number of organic acids and amino acids. menaquinone 6 (mk-6) was the major respira ... | 2009 | 19605713 |
| evaluation of the production of paralytic shellfish poisoning toxins by extracellular bacteria isolated from the toxic dinoflagellate alexandrium minutum. | the purpose of the study was to determine if paralytic shellfish poisoning (psp) toxins are present in extracellular bacteria isolated from a toxic strain of the dinoflagellate alexandrium minutum. a quantitative analysis was carried out of viable culturable bacteria attached to the surface of dinoflagellates and of bacteria present in dinoflagellate culture medium. a numerical taxonomy study was undertaken for presumptive identification of bacteria attached to the surface of dinoflagellates. me ... | 2009 | 19898534 |
| complete genome sequence of cellulophaga algicola type strain (ic166). | cellulophaga algicola bowman 2000 belongs to the family flavobacteriaceae within the phylum 'bacteroidetes' and was isolated from melosira collected from the eastern antarctic coastal zone. the species is of interest because its members produce a wide range of extracellular enzymes capable of degrading proteins and polysaccharides with temperature optima of 20-30°c. this is the first completed genome sequence of a member of the genus cellulophaga. the 4,888,353 bp long genome with its 4,285 prot ... | 2011 | 21475589 |
| combinatorial materials research applied to the development of new surface coatings xv: an investigation of polysiloxane anti-fouling/fouling-release coatings containing tethered quaternary ammonium salt groups. | as part of ongoing efforts aimed at the development of extensive structure-property relationships for moisture-curable polysiloxane coatings containing tethered quaternary ammonium salt (qas) moieties for potential application as environmental friendly coatings to combat marine biofouling, a combinatorial/high-throughput (c/ht) study was conducted that was focused on four different compositional variables. the coatings that were investigated were derived from solution blends of a silanol-termina ... | 2011 | 21480666 |
| composition and variability of biofouling organisms in seawater reverse osmosis desalination plants. | seawater reverse osmosis (swro) membrane biofouling remains a common challenge in desalination industry. however, the marine bacterial community that causes membrane fouling is poorly understood. microbial communities at different stage of treatment processes (intake, cartridge filtration and swro) of a desalination pilot plant were examined by both culture-based and culture-independent approaches. bacterial isolates were identified to match genus shewanella, alteromonas, vibrio and cellulophaga ... | 2011 | 21551282 |
| complete genome sequence of cellulophaga lytica type strain (lim-21). | cellulophaga lytica (lewin 1969) johansen et al. 1999 is the type species of the genus cellulophaga, which belongs to the family flavobacteriaceae within the phylum 'bacteroidetes' and was isolated from marine beach mud in limon, costa rica. the species is of biotechnological interest because its members produce a wide range of extracellular enzymes capable of degrading proteins and polysaccharides. after the genome sequence of cellulophaga algicola this is the second completed genome sequence o ... | 2011 | 21677859 |
| cellulophaga geojensis sp. nov., a member of the family flavobacteriaceae isolated from a marine sand. | a gram-staining-negative, aerobic, non-flagellated, non-spore-forming motile bacterial strain by gliding, designated m-m6t, was isolated from marine sand of geoje, an island of korea. strain m-m6t grew optimally at 25 -¦c, at ph 7.0-8.0 and in the presence of 2 % (w/v) nacl. phylogenetic analyses based on 16s rrna gene sequences revealed that strain m-m6t fell within the clade comprising cellulophaga species, forming a coherent cluster with cellulophaga lytica atcc 23178t and cellulophaga fucico ... | 2011 | 21828016 |
| the structural diversity of carbohydrate antigens of selected gram-negative marine bacteria. | marine microorganisms have evolved for millions of years to survive in the environments characterized by one or more extreme physical or chemical parameters, e.g., high pressure, low temperature or high salinity. marine bacteria have the ability to produce a range of biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents, and as a result, they have been a topic of research interest for many years. among these biologically active molecules, t ... | 2011 | 22073003 |