| heterologous dna uptake in cultured symbiodinium spp. aided by agrobacterium tumefaciens. | plant-targeted pcb302 plasmids containing sequences encoding gfp fusions with a microtubule-binding domain; gfp with the fimbrin actin-binding domain 2; and gfp with atrack1c from arabidopsis thaliana, all harbored in agrobacterium tumefaciens, were used to assay heterologous expression on three different clades of the photosynthetic dinoflagellate, symbiodinium. accessibility to the resistant cell wall and through the plasma membrane of these dinoflagellates was gained after brief but vigorous ... | 2015 | 26167858 |
| the psbo homolog from symbiodinium kawagutii (dinophyceae) characterized using biochemical and molecular methods. | a photosystem ii component, the psbo protein is essential for maximum rates of oxygen production during photosynthesis, and has been extensively characterized in plants and cyanobacteria but not in symbiotic dinoflagellates. its close interaction with d1 protein has important environmental implications since d1 has been identified as the primary site of damage in endosymbiotic dinoflagellates after thermal stress. we identified and biochemically characterized the psbo homolog from symbiodinium k ... | 2013 | 23708979 |
| proof that dinoflagellate spliced leader (dinosl) is a useful hook for fishing dinoflagellate transcripts from mixed microbial samples: symbiodinium kawagutii as a case study. | the ability to analyze dinoflagellate lineage-specific transcriptomes in the natural environment would be powerful for gaining understanding on how these organisms thrive in diverse environments and how they form harmful algal blooms and produce biotoxins. this can be made possible by lineage-specific mrna markers such as the dinoflagellate-specific trans-spliced leader (dinosl). by constructing and sequencing a 5'-cap selective full-length cdna library for a monoculture of the coral reef endosy ... | 2013 | 23773861 |
| the actin cytoskeleton organization and disorganization properties of the photosynthetic dinoflagellate symbiodinium kawagutii in culture. | the actin cytoskeleton organization in symbiotic marine dinoflagellates is largely undescribed; most likely, due to their intense pigment autofluorescence and cell walls that block fluorescent probe access. using a freeze-fracture and fixation procedure, we observed the actin cytoskeleton of symbiodinium kawagutii cultured in vitro with fluorescently labeled phalloidin and by indirect immunofluorescence with monoclonal antibodies specific for actin. the cytoskeleton appeared as an organized netw ... | 2014 | 25372347 |
| the symbiodinium kawagutii genome illuminates dinoflagellate gene expression and coral symbiosis. | dinoflagellates are important components of marine ecosystems and essential coral symbionts, yet little is known about their genomes. we report here on the analysis of a high-quality assembly from the 1180-megabase genome of symbiodinium kawagutii. we annotated protein-coding genes and identified symbiodinium-specific gene families. no whole-genome duplication was observed, but instead we found active (retro)transposition and gene family expansion, especially in processes important for successfu ... | 2015 | 26542574 |
| effects of trace metal concentrations on the growth of the coral endosymbiont symbiodinium kawagutii. | symbiodinium is an indispensable endosymbiont in corals and the most important primary producer in coral reef ecosystems. during the past decades, coral bleaching attributed to the disruption of the symbiosis has frequently occurred resulting in reduction of coral reef coverage globally. growth and proliferation of corals require some specific trace metals that are essential components of pertinent biochemical processes, such as in photosynthetic systems and electron transport chains. in additio ... | 2016 | 26903964 |
| genomes of coral dinoflagellate symbionts highlight evolutionary adaptations conducive to a symbiotic lifestyle. | despite half a century of research, the biology of dinoflagellates remains enigmatic: they defy many functional and genetic traits attributed to typical eukaryotic cells. genomic approaches to study dinoflagellates are often stymied due to their large, multi-gigabase genomes. members of the genus symbiodinium are photosynthetic endosymbionts of stony corals that provide the foundation of coral reef ecosystems. their smaller genome sizes provide an opportunity to interrogate evolution and functio ... | 2016 | 28004835 |