| systematic study of the genus acetobacter with descriptions of acetobacter indonesiensis sp. nov., acetobacter tropicalis sp. nov., acetobacter orleanensis (henneberg 1906) comb. nov., acetobacter lovaniensis (frateur 1950) comb. nov., and acetobacter estunensis (carr 1958) comb. nov. | thirty-one acetobacter strains obtained from culture collections and 45 acetobacter strains isolated from indonesian sources were investigated for their phenotypic characteristics, ubiquinone systems, dna base compositions, and levels of dna-dna relatedness. of 31 reference strains, six showed the presence of ubiquinone 10 (q-10). these strains were eliminated from the genus acetobacter. the other 25 reference strains and 45 indonesian isolates were subjected to a systematic study and separated ... | 2000 | 12483588 |
| increased internal and external bacterial load during drosophila aging without life-span trade-off. | the role of microbial load during aging of the adult fruit fly drosophila melanogaster is incompletely understood. here we show dramatic increases in aerobic and anaerobic bacterial load during aging, both inside the body and on the surface. scanning electron microscopy and cell staining analyses of the surface of aged flies detected structures resembling abundant small bacteria and bacterial biofilms. bacteria cultured from laboratory flies included aerobic species acetobacter aceti, acetobacte ... | 2007 | 17681150 |
| Global analysis of the genes involved in the thermotolerance mechanism of thermotolerant Acetobacter tropicalis SKU1100. | Acetobacter tropicalis SKU1100 is a thermotolerant acetic acid bacterium that grows even at 42 °C, a much higher temperature than the limit for the growth of mesophilic strains. To elucidate the mechanism underlying the thermotolerance of this strain, we attempted to identify the genes essential for growth at high temperature by transposon (Tn10) mutagenesis followed by gene or genome analysis. Among the 4,000 Tn10-inserted mutants obtained, 32 exhibited a growth phenotype comparable to that of ... | 2011 | 21979075 |
| dynamics and biodiversity of populations of lactic acid bacteria and acetic acid bacteria involved in spontaneous heap fermentation of cocoa beans in ghana. | the ghanaian cocoa bean heap fermentation process was studied through a multiphasic approach, encompassing both microbiological and metabolite target analyses. a culture-dependent (plating and incubation, followed by repetitive-sequence-based pcr analyses of picked-up colonies) and culture-independent (denaturing gradient gel electrophoresis [dgge] of 16s rrna gene amplicons, pcr-dgge) approach revealed a limited biodiversity and targeted population dynamics of both lactic acid bacteria (lab) an ... | 2007 | 17277227 |
| the microbiology of ghanaian cocoa fermentations analysed using culture-dependent and culture-independent methods. | export of cocoa beans is of great economic importance in ghana and several other tropical countries. raw cocoa has an astringent unpleasant taste and a spontaneous fermentation is the first step in a process leading to cocoa beans with the characteristic cocoa flavour and taste. the microbiology of ghanaian cocoa fermentations was investigated using culture-dependent and culture-independent methods. samples were collected at 12 hour intervals during 96-144 hour tray and traditional heap fermenta ... | 2007 | 17161485 |
| conversion of capsular polysaccharide, involved in pellicle formation, to extracellular polysaccharide by gale deletion in acetobacter tropicalis. | acetobacter tropicalis sku1100 produces a pellicle-forming capsular polysaccharide (cps), consisting of galactose, glucose, and rhamnose. we cloned the gale gene, a udp-galactose synthesis gene, from a. tropicalis sku1100 by pcr. a gale-disruptant was prepared and found not to produce cps and thus not to form a pellicle under the static condition. instead, the deltagale mutant secreted an extracellular polysaccharide (eps), which was purified and found to have a unique character, different from ... | 2006 | 17031049 |
| characterization and spontaneous mutation of a novel gene, pole, involved in pellicle formation in acetobacter tropicalis sku1100. | acetobacter tropicalis sku1100 produces a pellicle polysaccharide, consisting of galactose, glucose and rhamnose, which attaches to the cell surface. this strain forms two types of colony on agar plates: a rough-surfaced colony (r strain) and a mucoid smooth-surfaced colony (s strain). the r strain forms a pellicle, allowing it to float on the medium surface in static culture, while the s strain does not. the pellicle is an assemblage of cells which are tightly associated with capsular polysacch ... | 2005 | 16339956 |
| re-examination of the genus acetobacter, with descriptions of acetobacter cerevisiae sp. nov. and acetobacter malorum sp. nov. | thirty-four acetobacter strains, representing acetobacter aceti, acetobacter pasteurianus, acetobacter pomorum, acetobacter peroxydans, acetobacter lovaniensis, acetobacter estunensis, acetobacter orleanensis, acetobacter indonesiensis and acetobacter tropicalis, were subjected to a polyphasic study that included dna-dna hybridizations, dna base ratio determinations, 16s rdna sequence analysis and phenotypic characterization. two novel species are proposed, acetobacter cerevisiae sp. nov. and ac ... | 2002 | 12361257 |
| characterization and fibrinolytic activity of acetobacter sp. fp1 isolated from fermented pine needle extract. | the strain kctc 11629bp, isolated from spontaneously fermented pine needle extract (fpe), showed fibrinolysis activity. the isolated strain was analyzed in physiological and biochemical experiments. based on 16s rdna sequencing and phylogenic tree analysis, the strain was identified to be a part of the genus acetobacter, with acetobacter senegalensis and acetobacter tropicalis as the closest phylogenetic neighbors. based on genotypic and phenotypic results, it was proposed that bacterial strain ... | 2012 | 22370351 |
| draft genome sequence of acetobacter tropicalis type strain nbrc16470, a producer of optically pure d-glyceric acid. | here we report the 3.7-mb draft genome sequence of acetobacter tropicalis nbrc16470(t), which can produce optically pure d-glyceric acid (d-ga; 99% enantiomeric excess) from raw glycerol feedstock derived from biodiesel fuel production processes. | 2014 | 25523780 |
| a novel na(+)(k(+))/h(+) antiporter plays an important role in the growth of acetobacter tropicalis sku1100 at high temperatures via regulation of cation and ph homeostasis. | a gene encoding a putative na(+)/h(+) antiporter was previously proposed to be involved in the thermotolerance mechanism of acetobacter tropicalis sku 1100. the results of this study show that disruption of this antiporter gene impaired growth at high temperatures with an external ph>6.5. the growth impairment at high temperatures was much more severe in the absence of na(+) (with only the presence of k(+)); under these conditions, cells failed to grow even at 30°c and neutral to alkaline ph val ... | 2015 | 26100236 |
| consumption of dietary sugar by gut bacteria determines drosophila lipid content. | gut microorganisms are essential for the nutritional health of many animals, but the underlying mechanisms are poorly understood. this study investigated how lipid accumulation by adult drosophila melanogaster is reduced in flies associated with the bacterium acetobacter tropicalis which displays oral-faecal cycling between the gut and food. we demonstrate that the lower lipid content of a. tropicalis-colonized flies relative to bacteria-free flies is linked with a parallel bacterial-mediated re ... | 2015 | 26382071 |
| host genetic control of the microbiota mediates the drosophila nutritional phenotype. | a wealth of studies has demonstrated that resident microorganisms (microbiota) influence the pattern of nutrient allocation to animal protein and energy stores, but it is unclear how the effects of the microbiota interact with other determinants of animal nutrition, including animal genetic factors and diet. here, we demonstrate that members of the gut microbiota in drosophila melanogaster mediate the effect of certain animal genetic determinants on an important nutritional trait, triglyceride ( ... | 2015 | 26567306 |
| bacterial diversity of floor drain biofilms and drain waters in a listeria monocytogenes contaminated food processing environment. | sanitation protocols are applied on a daily basis in food processing facilities to prevent the risk of cross-contamination with spoilage organisms. floor drain water serves along with product-associated samples (slicer dust, brine or cheese smear) as an important hygiene indicator in monitoring listeria monocytogenes in food processing facilities. microbial communities of floor drains are representative for each processing area and are influenced to a large degree by food residues, liquid efflue ... | 2016 | 26881738 |
| microbiological characterization of traditional dough fermentation starter (jiaozi) for steamed bread making by culture-dependent and culture-independent methods. | in this study, the microbial composition of two types of jiaozi (a dough fermentation starter in making steamed bread) was investigated using both culture-dependent and culture-independent (pcr-dgge) methods. the numbers of the cultivable bacteria on mrs at 30°c and yeast on ypd at 28°c in the maize flour jiaozi (mfj) were 9.21±0.16 log cfu/g and 9.18±0.05 log cfu/g, respectively, which were higher than that in the rice flour jiaozi (rfj) (p<0.05). a total of 140 bacteria and 124 yeasts were iso ... | 2016 | 27351835 |
| the microbiology of bandji, palm wine of borassus akeassii from burkina faso: identification and genotypic diversity of yeasts, lactic acid and acetic acid bacteria. | to investigate physicochemical characteristics and especially genotypic diversity of the main culturable micro-organisms involved in fermentation of sap from borassus akeassii, a newly identified palm tree from west africa. | 2012 | 22979949 |
| microbiological and physicochemical characterization of small-scale cocoa fermentations and screening of yeast and bacterial strains to develop a defined starter culture. | spontaneous cocoa bean fermentations performed under bench- and pilot-scale conditions were studied using an integrated microbiological approach with culture-dependent and culture-independent techniques, as well as analyses of target metabolites from both cocoa pulp and cotyledons. both fermentation ecosystems reached equilibrium through a two-phase process, starting with the simultaneous growth of the yeasts (with saccharomyces cerevisiae as the dominant species) and lactic acid bacteria (lab) ... | 2012 | 22636007 |
| complete genome sequencing and comparative genomic analysis of the thermotolerant acetic acid bacterium, acetobacter pasteurianus sku1108, provide a new insight into thermotolerance. | acetobacter pasteurianus sku1108 is a typical thermotolerant acetic acid bacterium. in this study, the complete genome sequence of the sku1108 strain was elucidated, and information on genomic modifications due to the thermal adaptation of sku1108 was updated. in order to obtain a clearer understanding of the genetic background responsible for thermotolerance, the sku1108 genome was compared with those of two closely related complete genome strains, thermotolerant a. pasteurianus 386b and mesoph ... | 2016 | 27667143 |
| spontaneous cocoa bean fermentation carried out in a novel-design stainless steel tank: influence on the dynamics of microbial populations and physical-chemical properties. | spontaneous cocoa bean fermentations carried out in a novel-design 40-kg-capacity stainless steel tank (sst) was studied in parallel to traditional brazilian methods of fermentation in wooden boxes (40-kg-capacity wooden boxes (wb1) and 600-kg-capacity wooden boxes (wb2)) using a multiphasic approach that entailed culture-dependent and -independent microbiological analyses of fermenting cocoa bean pulp samples and target metabolite analyses of both cocoa pulp and cotyledons. both microbiological ... | 2012 | 23279821 |
| membrane-bound alcohol dehydrogenase is essential for glyceric acid production in acetobacter tropicalis. | acetobacter tropicalis nbrc16470 can produce highly enantiomerically pure d-glyceric acid (d-ga; >99 % enantiomeric excess) from glycerol. to investigate whether membrane-bound alcohol dehydrogenase (madh) is involved in ga production in a. tropicalis, we amplified part of the gene encoding madh subunit i (adha) using polymerase chain reaction and constructed an adha-disrupted mutant of a. tropicalis (δadha). because δadha did not produce ga, we confirmed that madh is essential for the conversio ... | 2011 | 21852749 |
| diversity of acetic acid bacteria present in healthy grapes from the canary islands. | the identification of acetic acid bacteria (aab) from sound grapes from the canary islands is reported in the present study. no direct recovery of bacteria was possible in the most commonly used medium, so microvinifications were performed on grapes from tenerife, la palma and lanzarote islands. up to 396 aab were isolated from those microvinifications and identified by 16s rrna gene sequencing and phylogenetic analysis. with this method, acetobacter pasteurianus, acetobacter tropicalis, glucono ... | 2011 | 21903289 |
| increased number of arginine-based salt bridges contributes to the thermotolerance of thermotolerant acetic acid bacteria, acetobacter tropicalis sku1100. | thermotolerant acetic acid bacteria (aab), acetobacter tropicalis sku1100, can grow above 40°c. to investigate the basis of its thermotolerance, we compared the genome of a. tropicalis sku1100 with that of mesophilic aab strain acetobacter pasteurianus ifo3283-01. the comparative genomic study showed that amino acid substitutions from large to small residue and lys to arg occur in many orthologous genes. furthermore, comparative modeling study was carried out with the orthologous proteins betwee ... | 2011 | 21554859 |
| two-stage electrodialytic concentration of glyceric acid from fermentation broth. | the aim of this research was the application of a two-stage electrodialysis (ed) method for glyceric acid (ga) recovery from fermentation broth. first, by desalting ed, glycerate solutions (counterpart is na+) were concentrated using ion-exchange membranes, and the glycerate recovery and energy consumption became more efficient with increasing the initial glycerate concentration (30 to 130 g/l). second, by water-splitting ed, the concentrated glycerate was electroconverted to ga using bipolar me ... | 2010 | 20674487 |
| microbial production of glyceric acid, an organic acid that can be mass produced from glycerol. | glyceric acid (ga), an unfamiliar biotechnological product, is currently produced as a small by-product of dihydroxyacetone production from glycerol by gluconobacter oxydans. we developed a method for the efficient biotechnological production of ga as a target compound for new surplus glycerol applications in the biodiesel and oleochemical industries. we investigated the ability of 162 acetic acid bacterial strains to produce ga from glycerol and found that the patterns of productivity and enant ... | 2009 | 19837846 |
| application of electrodialysis to glycerate recovery from a glycerol containing model solution and culture broth. | glyceric acid is produced by the conversion of glycerol via bioprocesses. the glycerate recovery from model solutions and from real culture broth was demonstrated by a desalting electrodialysis (ed) method. the addition of several impurities in glycerate model solutions, such as polypepton or yeast extract, did not have significant adverse effects on the whole ed process, and more than 93% of the glycerol added in the model solutions (50-150 g/l) was excluded. using culture broth of acetobacter ... | 2009 | 19332303 |
| acetobacter tropicalis is a major symbiont of the olive fruit fly (bactrocera oleae). | following cultivation-dependent and -independent techniques, we investigated the microbiota associated with bactrocera oleae, one of the major agricultural pests in olive-producing countries. bacterial 16s rrna gene libraries and ultrastructural analyses revealed the presence of several bacterial taxa associated with this insect, among which acetobacter tropicalis was predominant. the recent increased detection of acetic acid bacteria as symbionts of other insect model organisms, such as anophel ... | 2009 | 19304818 |
| biotransformation of glycerol to d-glyceric acid by acetobacter tropicalis. | bacterial strains capable of converting glycerol to glyceric acid (ga) were screened among the genera acetobacter and gluconacetobacter. most of the tested acetobacter and gluconacetobacter strains could produce 1.8 to 9.3 g/l ga from 10% (v/v) glycerol when intact cells were used as the enzyme source. acetobacter tropicalis nbrc16470 was the best ga producer and was therefore further investigated. based on the results of high-performance liquid chromatography analysis and specific rotation, the ... | 2009 | 18853153 |