| molecular cloning and characterization of a cellulase gene from a symbiotic protist of the lower termite, coptotermes formosanus. | the endo-beta-1,4-glucanase gene was cloned from a cdna library constructed from the mixed population of symbiotic protists in the hindgut of the lower termite, coptotermes formosanus, using the lambda zap ii vector. the recombinant phage library was screened for cellulolytic activity by the congo red staining procedure. the nucleotide sequence comprised 941 nucleotides including a polya tail sequence and showed high sequence similarity with endoglucanase genes belonging to glycosyl hydrolase fa ... | 2005 | 15777663 |
| testing protozoacidal activity of ligand-lytic peptides against termite gut protozoa in vitro (protozoa culture) and in vivo (microinjection into termite hindgut). | we are developing a novel approach to subterranean termite control that would lead to reduced reliance on the use of chemical pesticides. subterranean termites are dependent on protozoa in the hindguts of workers to efficiently digest wood. lytic peptides have been shown to kill a variety of protozoan parasites (mutwiri et al. 2000) and also protozoa in the gut of the formosan subterranean termite, coptotermes formosanus (husseneder and collier 2009). lytic peptides are part of the nonspecific i ... | 2010 | 21248688 |
| identification of endosymbiotic methanogen and ectosymbiotic spirochetes of gut protists of the termite coptotermes formosanus. | prokaryotic associations with gut protists of the termite coptotermes formosanus were investigated based on 16s rrna gene sequences. an endosymbiotic methanogen of spirotrichonympha leidyi phylogenetically grouped with endosymbionts of other gut protists in the genus methanobrevibacter, seemed to be unrelated to the host protist phylogeny. three different lineages of ectosymbiotic spirochetes in the genus treponema were identified in single cells of holomastigotoides mirabile, indicating their s ... | 2008 | 21558694 |
| phylogenetic identification of hypermastigotes, pseudotrichonympha, spirotrichonympha, holomastigotoides, and parabasalian symbionts in the hindgut of termites. | the phylogenetic diversity of parabasalian flagellates was examined based on the sequences of small subunit ribosomal rna genes amplified directly from the mixed population of flagellates in the hindgut of lower termites. in total, 33 representative sequences of parabasalids were recovered from eight termite species. fluorescent-labeled oligonucleotide probes specific for certain sequences were designed and used for the in situ identification of parabasalian species by whole-cell hybridization. ... | 2009 | 10847341 |
| influence of the diet components on the symbiotic microorganisms community in hindgut of coptotermes formosanus shiraki. | artificial diet was developed for rearing of lower termites (workers) coptotermes formosanus. c. formosanus was fed with either wood powder of japanese red pine, cellulose, cellobiose, or glucose for 30 days. the effect of carbon sources in the diet on the structure and function of the symbiotic intestinal microbial community and on the physiological activity of c. formosanus was studied. three symbiont protozoa, pseudotrichonympha grassi, holomastigotoides hartmanni, and spirotrichonympha leidy ... | 2006 | 16520926 |