| new endo-beta-1,4-glucanases from the parabasalian symbionts, pseudotrichonympha grassii and holomastigotoides mirabile of coptotermes termites. | abstract. an endo-beta-1,4-glucanase (eg) was purified from the hindgut of an australian mound-building termite, coptotermes lacteus. the hindgut extract had a peak separate from those for extracts obtained from the salivary glands and the midgut based on sephacryl s-200 gel chromatography, and also demonstrated an origin different from the endogenous egs of the termite itself. the recovery was further purified by sds-page, and its n-terminal amino acid sequence analyzed. this showed high homolo ... | 2002 | 12530528 |
| identification of endosymbiotic methanogen and ectosymbiotic spirochetes of gut protists of the termite coptotermes formosanus. | prokaryotic associations with gut protists of the termite coptotermes formosanus were investigated based on 16s rrna gene sequences. an endosymbiotic methanogen of spirotrichonympha leidyi phylogenetically grouped with endosymbionts of other gut protists in the genus methanobrevibacter, seemed to be unrelated to the host protist phylogeny. three different lineages of ectosymbiotic spirochetes in the genus treponema were identified in single cells of holomastigotoides mirabile, indicating their s ... | 2008 | 21558694 |
| purification and molecular cloning of xylanases from the wood-feeding termite, coptotermes formosanus shiraki. | coptotermes formosanus is one of the most destructive termites in the southern part of japan as well as in the united states. hemicellulose is a noncellulosic polysaccharide found in plant cell walls, and xylan is the major constituent of hemicellulose. since hemicellulose prevents access of cellulolytic enzymes to cellulose, enzymatic hydrolysis of hemicellulose is beneficial for cellulose digestion. we purified three functional xylanases to homogeneity from c. formosanus for the first time. el ... | 2009 | 19270398 |
| phylogenetic identification of hypermastigotes, pseudotrichonympha, spirotrichonympha, holomastigotoides, and parabasalian symbionts in the hindgut of termites. | the phylogenetic diversity of parabasalian flagellates was examined based on the sequences of small subunit ribosomal rna genes amplified directly from the mixed population of flagellates in the hindgut of lower termites. in total, 33 representative sequences of parabasalids were recovered from eight termite species. fluorescent-labeled oligonucleotide probes specific for certain sequences were designed and used for the in situ identification of parabasalian species by whole-cell hybridization. ... | 2009 | 10847341 |